Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Forward (+)

1245002 .. 1254033

Gene Structure 9032 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1003800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2757 bp

ATGGCGTCGCTCGCCTGGGTGATCTCCAGTAATCCCGGCCACGCGCTCTCCCCCAACATATTGGCTCACGACGCCAAGCCCAGGAAGATCTGCTACTCTCAATGCTCTACATTCTTTCTCCCTCCTCACCGCTCTCCCATTTGCAGAATGGGTTTTGTTTCTGGGCTGGAAAGGAAAAGGTCACTCAAAACCCGTATCTTTGCGTCAGTCACAGTAGATACTCCAGTAAATGCATACCCAGAGAAAGTTCAGCTTCCTAAAGGTGACTGTTGGTCCGTCCATAAATTTGGTGGCACCTGTGTGGGGAGTTCAGAAAGAATTAAGAATGTTGCAAAAATAATTCTTAGTGATGACTCAGAGAGGAAGATGGTAGTTGTCTCGGCAATGTCCAAGGTAACGGACATGATGTATGATCTTATCTACAAGGCTCAATCACGAGATGAGGCCTACATATCTGCATTAGATGCCGTTTTAGAAAAGCACAAATCTACAGCTCTTGATTTACTTGATGGGGATGATCTTAGTAGTTTCTTAGCACAATTGCATCACGACATCAGTAACTTGAAAGCGATGCTTCGTGCTATATACATTGCTGGTCATGCGACAGAATCGTTCACAGATTTTGTTGTCGGACATGGAGAGTTATGGTCTGCGCAGATGTTGTCATGTGTTATTAGAAAGAATGGGTTAGATTGCAATTGGATGGATACAAGGGAAGTGCTTATTGTAAATCCTACAAGTTCTAATCAAGTTGATCCTGATTTAAAGGAATCTGAAGACAGACTTGAGAAATGGTATTCCAAAAACCCATCAAAAACAATTGTAGCTGCCGGCTTCATTGCTAGCACACCTCAAAATATTCCTACTACTTTGAAGAGAGATGGAAGTGACTTCTCTGCAGCAATCATGGGTGCCTTATTCAAGGCCGGTCAAGTCACAATTTGGACAGATGTTGATGGAGTCTATAGTGCAGATCCCAGAAAAGTCAGTGAGGCAGTGATTCTGAAGACTCTGTCTTATCAAGAAGCCTGGGAAATGTCTTATTTTGGGGCAAATGTTCTGCACCCACGCACTATCATCCCAGTGATGCAATATGACATTCCAATTATAATAAGGAACGTTTTCAACCTTGCTGCACCTGGAACAAAAATTTGTCGCCCTACTGAGGATGAAACTGATCAGGGTTTGGAGTCGTTTGTCAAAGGATTTGCCACAATAGACAACTTGGCCCTTGTCAATGTGGAAGGAACTGGAATGGCTGGTGTTCCTGGTACAGCAAGTGATATATTCAGTGCTGTAAAGGATGTTGGGGCTAATGTTATTATGATATCTCAGGCTAGTAGTGAGCACTCTGTGTGCTTTGCTGTCCCTGAGAAGGAAGTAAATGCTGTTTCTGAGTTACTGCAGTCTAGATTTCGTGAGGCTTTGAATGCTGGGCGTCTTTCTCAGGTTCAAGTCATTCCAAACTGTAGTATTTTGGCAGCAGTTGGCCAAAAAATGGCCAGTACTCCAGGAGTTAGTGCAACTCTTTTCAATGCACTGGCAAAGGCAAACATTAATGTGCGTGCAATTGCTCAGGGTTGTACTGAGTACAATATTACTGTAGTGATAAAGCGGGAGGATTGTATTAGGGCTCTGAGAGCTGTCCATTCTAGATTCTATCTTTCAAGAACCACAATAGCGGTGGGCATCATCGGACCTGGTCTGATTGGTGCCACATTACTAGATCAATTAAGGGATCAGGCAGCTACCCTCAAGGAAGAGTTTAACATCGATTTGCGTGTTATGGGAATCACTGGCTCACGGACAATGCTTTTGAGTGAGGCGGGTGTTGACTTATCTAGATGGAGAGAACTTCAAAAAGAGAAAGGAGCTGTGGCAGATATGGAAAAGTTTGTGCAGCATGTACATGGGAATCATTTTATTCCAAATACTGTTTTGGTTGACTGCACAGCTGACTCTAGTATTGCAAGCCATTACTATGACTGGTTGCGGAAAGGAATTCACGTAGTCACTCCCAACAAAAAGGCCAATTCAGGGCCGCTTGATCAATATTTAAAATTACGAGCTCTTCAACGGCAATCATATACACATTACTTCTATGAAGCTACTGTTGGAGCTGGTCTGCCAATTATTAATACATTACAAGGTCTCCTCGAAACTGGAGACAAAATATTGCGTATTGAGGGCATCTTCAGTGGTACTCTGAGTTATATCTTCAACAATTTCACTGGAGGACGCACTTTTAGTGAGGTGGTGGCTGAAGCAAAGCAGGCAGGTTATACTGAGCCGGATCCAAGGGATGACTTATCTGGAACAGATGTTTGCAGAAAGGTGATAATTCTTGCTAGAGAATCTGGTCTTAAGCTGGAGCTCTCTGACATCCCTGTTGAAAGCCTTGTGCCGGAACCGTTGAAGGATAGTGCATCAGCCGAGGAATTCATGCAAAAACTTCCACAATTTGATCAAGAGATGAGTAAGAAAAGAGAGATAGCTGAGGATGCAGGAGGAGTCTTGAGATTCGTTGGGGTGGTTGACATGGTCAATCAGAAAGGGGAAGTGAAGCTGCAGACGTACAAGAATGATCATCCATTTGCTCAGCTTTCCGGGGCAGATAATATAATAGCGTTCACAACAACTAGATACAAAGCTCAACCCCTAATAATACGTGGACCTGGTGCTGGTGCTGAAGTCACTGCTGGTGGAGTCTTCAGTGACCTGTTGCGGCTTGCTTCGTACCTTGGTGCCCCGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

919

Amino Acids

100.38

Weight (kDa)

6.29

Isoelectric Point (pI)

35.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	93 - 373	8.4e-43	Amino acid kinase family
ACT_9	PF22468	412 - 472	3.1e-17	ACT domain
ACT_7	PF13840	487 - 551	2e-10	ACT domain
ACT_9	PF22468	494 - 553	1.8e-21	ACT domain
ACT	PF01842	500 - 541	2e-06	ACT domain
NAD_binding_3	PF03447	566 - 702	1.7e-22	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	710 - 908	7.2e-53	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1109
Acc16I	TGCGCA	1 cut(s)	654
Acc36I	ACCTGC	1 cut(s)	2267
AccB1I	GGYRCC	4 cut(s)	293, 911, 1712, 2744
AccB7I	CCANNNNNTGG	1 cut(s)	1498
AccBSI	CCGCTC	1 cut(s)	132
AccII	CGCG	1 cut(s)	44
AciI	CCGC	7 cut(s)	130, 1615, 1682, 1826, 1993, 2042, 2725
AclI	AACGTT	1 cut(s)	1119
AclWI	GGATC	5 cut(s)	749, 968, 1746, 2288, 2301
AcoI	YGGCCR	3 cut(s)	37, 1489, 1500
AcsI	RAATTY	4 cut(s)	284, 1149, 2001, 2438
AcuI	CTGAAG	6 cut(s)	795, 1025, 2179, 2283, 2695, 2709
AcyI	GRCGYC	3 cut(s)	5, 72, 1438
AdeI	CACNNNGTG	1 cut(s)	1354
AfaI	GTAC	8 cut(s)	1273, 1507, 1585, 1592, 1908, 2203, 2576, 2738
AfiI	CCNNNNNNNGG	8 cut(s)	81, 303, 1165, 1375, 1498, 2037, 2404, 2681
AflII	CTTAAG	1 cut(s)	2363
AhdI	GACNNNNNGTC	1 cut(s)	271
AjnI	CCWGG	8 cut(s)	14, 80, 1028, 1138, 1267, 1510, 1699, 2674
AjuI	GAANNNNNNNTTGG	2 cut(s)	2097, 2129
AleI	CACNNNNGTG	1 cut(s)	299
Alw21I	GWGCWC	3 cut(s)	1350, 2071, 2376
Alw26I	GTCTC	3 cut(s)	382, 2156, 2160
AlwI	GGATC	5 cut(s)	749, 968, 1746, 2288, 2301
AoxI	GGCC	8 cut(s)	37, 444, 924, 1227, 1489, 1500, 2028, 2039
ApeKI	GCWGC	7 cut(s)	827, 899, 1133, 1481, 1745, 1900, 2566
ApoI	RAATTY	4 cut(s)	284, 1149, 2001, 2438
ArsI	GACNNNNNNTTYG	2 cut(s)	1798, 1830
AseI	ATTAAT	2 cut(s)	1557, 2136
Asp700I	GAANNNNTTC	1 cut(s)	1121
AspLEI	GCGC	2 cut(s)	46, 655
AspS9I	GGNCC	5 cut(s)	273, 1228, 1697, 2039, 2672
AsuC2I	CCSGG	2 cut(s)	36, 2608
AsuHPI	GGTGA	4 cut(s)	31, 119, 275, 2347
AsuNHI	GCTAGC	1 cut(s)	842
AvaII	GGWCC	3 cut(s)	273, 1697, 2672
BaeGI	GKGCMC	1 cut(s)	2749
BaeI	ACNNNNGTAYC	2 cut(s)	210, 243
BalI	TGGCCA	2 cut(s)	1491, 1502
BamHI	GGATCC	1 cut(s)	2293
BanI	GGYRCC	4 cut(s)	293, 911, 1712, 2744
BanII	GRGCYC	3 cut(s)	1636, 2071, 2376
BarI	GAAGNNNNNNTAC	2 cut(s)	77, 109
BauI	CACGAG	1 cut(s)	435
BbsI	GAAGAC	3 cut(s)	783, 1013, 2701
Bbv12I	GWGCWC	3 cut(s)	1350, 2071, 2376
BbvCI	CCTCAGC	1 cut(s)	2496
BbvI	GCAGC	7 cut(s)	814, 911, 1120, 1493, 1757, 1912, 2553
BccI	CCATC	7 cut(s)	361, 503, 697, 817, 875, 950, 1839
BceAI	ACGGC	2 cut(s)	452, 2093
BciT130I	CCWGG	8 cut(s)	16, 82, 1030, 1140, 1269, 1512, 1701, 2676
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	4 cut(s)	1177, 2047, 2464, 2584
BcnI	CCSGG	2 cut(s)	36, 2608
BcoDI	GTCTC	3 cut(s)	382, 2156, 2160
BfaI	CTAG	9 cut(s)	843, 1338, 1410, 1653, 1724, 1842, 1962, 2349, 2640
BfmI	CTRYAG	7 cut(s)	489, 897, 964, 1403, 1468, 1602, 2567
BfrI	CTTAAG	1 cut(s)	2363
BfuAI	ACCTGC	1 cut(s)	2267
BfuI	GTATCC	1 cut(s)	700
BglII	AGATCT	1 cut(s)	87
BisI	GCNGC	9 cut(s)	828, 900, 1134, 1482, 1746, 1901, 2042, 2567, 2726
BlpI	GCTNAGC	1 cut(s)	2598
BlsI	GCNGC	9 cut(s)	829, 901, 1135, 1483, 1747, 1902, 2043, 2568, 2727
BmcAI	AGTACT	1 cut(s)	1507
Bme18I	GGWCC	3 cut(s)	273, 1697, 2672
BmeRI	GACNNNNNGTC	1 cut(s)	271
BmgT120I	GGNCC	5 cut(s)	273, 1228, 1697, 2039, 2672
BmiI	GGNNCC	6 cut(s)	295, 913, 1714, 2295, 2409, 2746
BmrI	ACTGGG	1 cut(s)	1076
BmsI	GCATC	8 cut(s)	454, 553, 561, 1077, 1698, 2199, 2435, 2491
BmtI	GCTAGC	1 cut(s)	846
BmuI	ACTGGG	1 cut(s)	1076
BpiI	GAAGAC	3 cut(s)	783, 1013, 2701
BpmI	CTGGAG	6 cut(s)	10, 207, 1494, 2184, 2253, 2390
Bpu10I	CCTNAGC	2 cut(s)	1575, 2496
Bpu1102I	GCTNAGC	1 cut(s)	2598
BpuEI	CTTGAG	3 cut(s)	806, 1739, 2536
BpuMI	CCSGG	2 cut(s)	36, 2608
Bsa29I	ATCGAT	1 cut(s)	1773
BsaAI	YACGTR	2 cut(s)	2008, 2669
BsaHI	GRCGYC	3 cut(s)	5, 72, 1438
BsaI	GGTCTC	1 cut(s)	2156
BsaJI	CCNNGG	8 cut(s)	15, 80, 390, 1029, 2297, 2433, 2607, 2740
BsaXI	ACNNNNNCTCC	4 cut(s)	32, 62, 2136, 2166
Bsc4I	CCNNNNNNNGG	8 cut(s)	81, 303, 1165, 1375, 1498, 2037, 2404, 2681
Bse118I	RCCGGY	2 cut(s)	830, 926
Bse3DI	GCAATG	3 cut(s)	390, 588, 837
BseBI	CCWGG	8 cut(s)	16, 82, 1030, 1140, 1269, 1512, 1701, 2676
BseCI	ATCGAT	1 cut(s)	1773
BseDI	CCNNGG	8 cut(s)	15, 80, 390, 1029, 2297, 2433, 2607, 2740
BseGI	GGATG	9 cut(s)	520, 708, 1077, 1174, 1309, 2308, 2382, 2506, 2587
BseLI	CCNNNNNNNGG	8 cut(s)	81, 303, 1165, 1375, 1498, 2037, 2404, 2681
BseMI	GCAATG	3 cut(s)	390, 588, 837
BseRI	GAGGAG	3 cut(s)	114, 2144, 2523
BseSI	GKGCMC	1 cut(s)	2749
BseXI	GCAGC	7 cut(s)	814, 911, 1120, 1493, 1757, 1912, 2553
BseYI	CCCAGC	1 cut(s)	1433
BsgI	GTGCAG	5 cut(s)	990, 1046, 1119, 1919, 1933
Bsh1236I	CGCG	1 cut(s)	44
BshFI	GGCC	8 cut(s)	39, 446, 926, 1229, 1491, 1502, 2030, 2041
BshNI	GGYRCC	4 cut(s)	293, 911, 1712, 2744
BshVI	ATCGAT	1 cut(s)	1773
BsiHKAI	GWGCWC	3 cut(s)	1350, 2071, 2376
BsiSI	CCGG	6 cut(s)	36, 831, 927, 2291, 2405, 2607
BslFI	GGGAC	1 cut(s)	1352
BslI	CCNNNNNNNGG	8 cut(s)	81, 303, 1165, 1375, 1498, 2037, 2404, 2681
BsmAI	GTCTC	3 cut(s)	382, 2156, 2160
BsmFI	GGGAC	1 cut(s)	1352
BsmI	GAATGC	1 cut(s)	1435
BsnI	GGCC	8 cut(s)	39, 446, 926, 1229, 1491, 1502, 2030, 2041
Bso31I	GGTCTC	1 cut(s)	2156
Bsp1286I	GDGCHC	5 cut(s)	1350, 1636, 2071, 2376, 2749
Bsp1407I	TGTACA	1 cut(s)	1906
Bsp1720I	GCTNAGC	1 cut(s)	2598
BspACI	CCGC	7 cut(s)	130, 1615, 1682, 1826, 1993, 2042, 2725
BspANI	GGCC	8 cut(s)	39, 446, 926, 1229, 1491, 1502, 2030, 2041
BspDI	ATCGAT	1 cut(s)	1773
BspFNI	CGCG	1 cut(s)	44
BspLI	GGNNCC	6 cut(s)	295, 913, 1714, 2295, 2409, 2746
BspMAI	CTGCAG	3 cut(s)	901, 1407, 2571
BspMI	ACCTGC	1 cut(s)	2267
BspOI	GCTAGC	1 cut(s)	846
BspPI	GGATC	5 cut(s)	749, 968, 1746, 2288, 2301
BspQI	GCTCTTC	1 cut(s)	2076
BspT107I	GGYRCC	4 cut(s)	293, 911, 1712, 2744
BspTI	CTTAAG	1 cut(s)	2363
BspTNI	GGTCTC	1 cut(s)	2156
BsrBI	CCGCTC	1 cut(s)	132
BsrDI	GCAATG	3 cut(s)	390, 588, 837
BsrFI	RCCGGY	2 cut(s)	830, 926
BsrGI	TGTACA	1 cut(s)	1906
BssAI	RCCGGY	2 cut(s)	830, 926
BssECI	CCNNGG	8 cut(s)	15, 80, 390, 1029, 2297, 2433, 2607, 2740
BssNI	GRCGYC	3 cut(s)	5, 72, 1438
BssSI	CACGAG	1 cut(s)	435
BssT1I	CCWWGG	3 cut(s)	390, 2297, 2740
Bst2BI	CACGAG	1 cut(s)	435
Bst2UI	CCWGG	8 cut(s)	16, 82, 1030, 1140, 1269, 1512, 1701, 2676
Bst4CI	ACNGT	7 cut(s)	214, 269, 1469, 1603, 1936, 2113, 2412
Bst6I	CTCTTC	3 cut(s)	869, 1755, 2076
BstACI	GRCGYC	3 cut(s)	5, 72, 1438
BstAFI	CTTAAG	1 cut(s)	2363
BstAPI	GCANNNNNTGC	1 cut(s)	464
BstAUI	TGTACA	1 cut(s)	1906
BstBAI	YACGTR	2 cut(s)	2008, 2669
BstC8I	GCNNGC	7 cut(s)	12, 832, 844, 1566, 1972, 2274, 2730
BstF5I	GGATG	9 cut(s)	520, 708, 1077, 1174, 1309, 2308, 2382, 2506, 2587
BstFNI	CGCG	1 cut(s)	44
BstHHI	GCGC	2 cut(s)	46, 655
BstMAI	GTCTC	3 cut(s)	382, 2156, 2160
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 71, 464, 599, 1430, 1640, 2273, 2501
BstNI	CCWGG	8 cut(s)	16, 82, 1030, 1140, 1269, 1512, 1701, 2676
BstNSI	RCATGY	1 cut(s)	1907
BstSFI	CTRYAG	7 cut(s)	489, 897, 964, 1403, 1468, 1602, 2567
BstSLI	GKGCMC	1 cut(s)	2749
BstUI	CGCG	1 cut(s)	44
BstV1I	GCAGC	7 cut(s)	814, 911, 1120, 1493, 1757, 1912, 2553
BstV2I	GAAGAC	3 cut(s)	783, 1013, 2701
BstX2I	RGATCY	3 cut(s)	87, 973, 2293
BstXI	CCANNNNNNTGG	2 cut(s)	61, 287
BstYI	RGATCY	3 cut(s)	87, 973, 2293
Bsu15I	ATCGAT	1 cut(s)	1773
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	8 cut(s)	39, 446, 926, 1229, 1491, 1502, 2030, 2041
BsuTUI	ATCGAT	1 cut(s)	1773
BtgZI	GCGATG	1 cut(s)	584
BtsCI	GGATG	9 cut(s)	520, 708, 1077, 1174, 1309, 2308, 2382, 2506, 2587
BtsI	GCAGTG	2 cut(s)	1002, 2694
BveI	ACCTGC	1 cut(s)	2267
Cac8I	GCNNGC	7 cut(s)	12, 832, 844, 1566, 1972, 2274, 2730
CfoI	GCGC	2 cut(s)	46, 655
Cfr10I	RCCGGY	2 cut(s)	830, 926
Cfr13I	GGNCC	5 cut(s)	273, 1228, 1697, 2039, 2672
ClaI	ATCGAT	1 cut(s)	1773
CseI	GACGC	4 cut(s)	80, 192, 1427, 2247
CsiI	ACCWGGT	2 cut(s)	1699, 2674
Csp6I	GTAC	8 cut(s)	1272, 1506, 1584, 1591, 1907, 2202, 2575, 2737
CspCI	CAANNNNNGTGG	2 cut(s)	1665, 1700
CviAII	CATG	9 cut(s)	403, 599, 635, 666, 907, 1904, 1910, 2443, 2539
CviQI	GTAC	8 cut(s)	1272, 1506, 1584, 1591, 1907, 2202, 2575, 2737
DraI	TTTAAA	2 cut(s)	765, 2058
DraIII	CACNNNGTG	1 cut(s)	1354
DriI	GACNNNNNGTC	1 cut(s)	271
EaeI	YGGCCR	3 cut(s)	37, 1489, 1500
Eam1104I	CTCTTC	3 cut(s)	869, 1755, 2076
Eam1105I	GACNNNNNGTC	1 cut(s)	271
EarI	CTCTTC	3 cut(s)	869, 1755, 2076
Ecl136II	GAGCTC	2 cut(s)	2069, 2374
Eco130I	CCWWGG	3 cut(s)	390, 2297, 2740
Eco147I	AGGCCT	1 cut(s)	446
Eco24I	GRGCYC	3 cut(s)	1636, 2071, 2376
Eco31I	GGTCTC	1 cut(s)	2156
Eco32I	GATATC	1 cut(s)	1329
Eco47I	GGWCC	3 cut(s)	273, 1697, 2672
Eco53kI	GAGCTC	2 cut(s)	2069, 2374
Eco57I	CTGAAG	6 cut(s)	795, 1025, 2179, 2283, 2695, 2709
EcoICRI	GAGCTC	2 cut(s)	2069, 2374
EcoRI	GAATTC	2 cut(s)	2001, 2438
EcoRII	CCWGG	8 cut(s)	14, 80, 1028, 1138, 1267, 1510, 1699, 2674
EcoRV	GATATC	1 cut(s)	1329
EcoT14I	CCWWGG	3 cut(s)	390, 2297, 2740
EcoT22I	ATGCAT	1 cut(s)	235
EcoT38I	GRGCYC	3 cut(s)	1636, 2071, 2376
ErhI	CCWWGG	3 cut(s)	390, 2297, 2740
FaeI	CATG	9 cut(s)	406, 602, 638, 669, 910, 1907, 1913, 2446, 2542
FalI	AAGNNNNNCTT	6 cut(s)	237, 269, 558, 590, 768, 800
FaqI	GGGAC	1 cut(s)	1352
FatI	CATG	9 cut(s)	402, 598, 634, 665, 906, 1903, 1909, 2442, 2538
FauI	CCCGC	2 cut(s)	1608, 1819
FbaI	TGATCA	4 cut(s)	1177, 2047, 2464, 2584
Fnu4HI	GCNGC	9 cut(s)	828, 900, 1134, 1482, 1746, 1901, 2042, 2567, 2726
FokI	GGATG	9 cut(s)	527, 715, 1064, 1181, 1316, 2315, 2369, 2513, 2574
FriOI	GRGCYC	3 cut(s)	1636, 2071, 2376
Fsp4HI	GCNGC	9 cut(s)	828, 900, 1134, 1482, 1746, 1901, 2042, 2567, 2726
FspBI	CTAG	9 cut(s)	843, 1338, 1410, 1653, 1724, 1842, 1962, 2349, 2640
FspI	TGCGCA	1 cut(s)	654
GlaI	GCGC	2 cut(s)	45, 654
GluI	GCNGC	9 cut(s)	828, 900, 1134, 1482, 1746, 1901, 2042, 2567, 2726
GsaI	CCCAGC	1 cut(s)	1437
GsuI	CTGGAG	6 cut(s)	10, 207, 1494, 2184, 2253, 2390
HaeIII	GGCC	8 cut(s)	39, 446, 926, 1229, 1491, 1502, 2030, 2041
HapII	CCGG	6 cut(s)	36, 831, 927, 2291, 2405, 2607
HgaI	GACGC	4 cut(s)	80, 192, 1427, 2247
HhaI	GCGC	2 cut(s)	46, 655
Hin1I	GRCGYC	3 cut(s)	5, 72, 1438
Hin1II	CATG	9 cut(s)	406, 602, 638, 669, 910, 1907, 1913, 2446, 2542
Hin6I	GCGC	2 cut(s)	44, 653
HinP1I	GCGC	2 cut(s)	44, 653
HincII	GTYRAC	3 cut(s)	1834, 1945, 2536
HindII	GTYRAC	3 cut(s)	1834, 1945, 2536
HpaII	CCGG	6 cut(s)	36, 831, 927, 2291, 2405, 2607
HphI	GGTGA	4 cut(s)	31, 119, 275, 2347
Hpy166II	GTNNAC	6 cut(s)	615, 1834, 1945, 2536, 2631, 2672
Hpy8I	GTNNAC	6 cut(s)	615, 1834, 1945, 2536, 2631, 2672
Hpy99I	CGWCG	2 cut(s)	10, 74
HpyAV	CCTTC	3 cut(s)	1238, 1369, 2410
HpyCH4III	ACNGT	7 cut(s)	214, 269, 1469, 1603, 1936, 2113, 2412
HpyCH4IV	ACGT	4 cut(s)	1119, 2007, 2573, 2668
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 71, 464, 599, 1430, 1640, 2273, 2501
HpySE526I	ACGT	4 cut(s)	1119, 2007, 2573, 2668
Hsp92I	GRCGYC	3 cut(s)	5, 72, 1438
Hsp92II	CATG	9 cut(s)	406, 602, 638, 669, 910, 1907, 1913, 2446, 2542
HspAI	GCGC	2 cut(s)	44, 653
KroI	GCCGGC	1 cut(s)	830
KroNI	GCCGGC	1 cut(s)	832
Ksp22I	TGATCA	4 cut(s)	1177, 2047, 2464, 2584
LguI	GCTCTTC	1 cut(s)	2076
LmnI	GCTCC	3 cut(s)	1871, 2117, 2371
Lsp1109I	GCAGC	7 cut(s)	814, 911, 1120, 1493, 1757, 1912, 2553
LweI	GCATC	8 cut(s)	454, 553, 561, 1077, 1698, 2199, 2435, 2491
MabI	ACCWGGT	2 cut(s)	1699, 2674
MaeI	CTAG	9 cut(s)	843, 1338, 1410, 1653, 1724, 1842, 1962, 2349, 2640
MaeII	ACGT	4 cut(s)	1119, 2007, 2573, 2668
MbiI	CCGCTC	1 cut(s)	132
MfeI	CAATTG	4 cut(s)	539, 697, 819, 1569
MflI	RGATCY	3 cut(s)	87, 973, 2293
MhlI	GDGCHC	5 cut(s)	1350, 1636, 2071, 2376, 2749
MlsI	TGGCCA	2 cut(s)	1491, 1502
MluNI	TGGCCA	2 cut(s)	1491, 1502
MlyI	GAGTC	7 cut(s)	347, 969, 1003, 1199, 1952, 2520, 2715
MmeI	TCCRAC	2 cut(s)	610, 2095
Mox20I	TGGCCA	2 cut(s)	1491, 1502
Mph1103I	ATGCAT	1 cut(s)	235
MroNI	GCCGGC	1 cut(s)	830
MroXI	GAANNNNTTC	1 cut(s)	1121
MscI	TGGCCA	2 cut(s)	1491, 1502
MseI	TTAA	8 cut(s)	321, 764, 1557, 1733, 1767, 2057, 2136, 2364
MslI	CAYNNNNRTG	5 cut(s)	299, 1082, 1559, 1908, 1980
Msp20I	TGGCCA	2 cut(s)	1491, 1502
MspA1I	CMGCKG	1 cut(s)	1955
MspCI	CTTAAG	1 cut(s)	2363
MspI	CCGG	6 cut(s)	36, 831, 927, 2291, 2405, 2607
MunI	CAATTG	4 cut(s)	539, 697, 819, 1569
Mva1269I	GAATGC	1 cut(s)	1435
MvaI	CCWGG	8 cut(s)	16, 82, 1030, 1140, 1269, 1512, 1701, 2676
MvnI	CGCG	1 cut(s)	44
MwoI	GCNNNNNNNGC	8 cut(s)	11, 71, 464, 599, 1430, 1640, 2273, 2501
NaeI	GCCGGC	1 cut(s)	832
NciI	CCSGG	2 cut(s)	36, 2608
NgoMIV	GCCGGC	1 cut(s)	830
NheI	GCTAGC	1 cut(s)	842
NlaIII	CATG	9 cut(s)	406, 602, 638, 669, 910, 1907, 1913, 2446, 2542
NlaIV	GGNNCC	6 cut(s)	295, 913, 1714, 2295, 2409, 2746
NmeAIII	GCCGAG	2 cut(s)	359, 2458
NmuCI	GTSAC	8 cut(s)	180, 208, 263, 887, 934, 2011, 2692, 2714
NsbI	TGCGCA	1 cut(s)	654
NsiI	ATGCAT	1 cut(s)	235
NspI	RCATGY	1 cut(s)	1907
OliI	CACNNNNGTG	1 cut(s)	299
PceI	AGGCCT	1 cut(s)	446
PciSI	GCTCTTC	1 cut(s)	2076
PctI	GAATGC	1 cut(s)	1435
PdiI	GCCGGC	1 cut(s)	832
PdmI	GAANNNNTTC	1 cut(s)	1121
PfeI	GAWTC	8 cut(s)	608, 770, 1000, 1656, 1791, 1915, 2354, 2520
PflFI	GACNNNGTC	3 cut(s)	1012, 1701, 2540
PflMI	CCANNNNNTGG	1 cut(s)	1498
PfoI	TCCNGGA	1 cut(s)	1510
PkrI	GCNGC	9 cut(s)	829, 901, 1135, 1483, 1747, 1902, 2043, 2568, 2727
PleI	GAGTC	7 cut(s)	347, 968, 1003, 1198, 1952, 2519, 2714
PpsI	GAGTC	7 cut(s)	347, 968, 1003, 1198, 1952, 2519, 2714
Ppu21I	YACGTR	2 cut(s)	2008, 2669
PshBI	ATTAAT	2 cut(s)	1557, 2136
PsiI	TTATAA	1 cut(s)	1109
Psp124BI	GAGCTC	2 cut(s)	2071, 2376
Psp1406I	AACGTT	1 cut(s)	1119
Psp6I	CCWGG	8 cut(s)	14, 80, 1028, 1138, 1267, 1510, 1699, 2674
PspFI	CCCAGC	1 cut(s)	1433
PspGI	CCWGG	8 cut(s)	14, 80, 1028, 1138, 1267, 1510, 1699, 2674
PspN4I	GGNNCC	6 cut(s)	295, 913, 1714, 2295, 2409, 2746
PspPI	GGNCC	5 cut(s)	273, 1228, 1697, 2039, 2672
PstI	CTGCAG	3 cut(s)	901, 1407, 2571
PsuI	RGATCY	3 cut(s)	87, 973, 2293
PsyI	GACNNNGTC	3 cut(s)	1012, 1701, 2540
PvuII	CAGCTG	1 cut(s)	1955
RsaI	GTAC	8 cut(s)	1273, 1507, 1585, 1592, 1908, 2203, 2576, 2738
RsaNI	GTAC	8 cut(s)	1272, 1506, 1584, 1591, 1907, 2202, 2575, 2737
RseI	CAYNNNNRTG	5 cut(s)	299, 1082, 1559, 1908, 1980
SacI	GAGCTC	2 cut(s)	2071, 2376
SapI	GCTCTTC	1 cut(s)	2076
SaqAI	TTAA	8 cut(s)	321, 764, 1557, 1733, 1767, 2057, 2136, 2364
SatI	GCNGC	9 cut(s)	828, 900, 1134, 1482, 1746, 1901, 2042, 2567, 2726
Sau96I	GGNCC	5 cut(s)	273, 1228, 1697, 2039, 2672
ScaI	AGTACT	1 cut(s)	1507
SchI	GAGTC	7 cut(s)	347, 969, 1003, 1199, 1952, 2520, 2715
SduI	GDGCHC	5 cut(s)	1350, 1636, 2071, 2376, 2749
SexAI	ACCWGGT	2 cut(s)	1699, 2674
SfaNI	GCATC	8 cut(s)	454, 553, 561, 1077, 1698, 2199, 2435, 2491
SfcI	CTRYAG	7 cut(s)	489, 897, 964, 1403, 1468, 1602, 2567
SinI	GGWCC	3 cut(s)	273, 1697, 2672
SmiMI	CAYNNNNRTG	5 cut(s)	299, 1082, 1559, 1908, 1980
SmlI	CTYRAG	4 cut(s)	785, 1754, 2363, 2515
SmoI	CTYRAG	4 cut(s)	785, 1754, 2363, 2515
SseBI	AGGCCT	1 cut(s)	446
SsiI	CCGC	7 cut(s)	130, 1615, 1682, 1826, 1993, 2042, 2725
SspI	AATATT	4 cut(s)	859, 1597, 2054, 2175
SspMI	CTAG	9 cut(s)	843, 1338, 1410, 1653, 1724, 1842, 1962, 2349, 2640
SstI	GAGCTC	2 cut(s)	2071, 2376
StuI	AGGCCT	1 cut(s)	446
StyI	CCWWGG	3 cut(s)	390, 2297, 2740
TaaI	ACNGT	7 cut(s)	214, 269, 1469, 1603, 1936, 2113, 2412
TaiI	ACGT	4 cut(s)	1122, 2010, 2576, 2671
TaqI	TCGA	2 cut(s)	1773, 2157
TatI	WGTACW	4 cut(s)	1505, 1583, 1590, 1906
TauI	GCSGC	2 cut(s)	2044, 2728
TfiI	GAWTC	8 cut(s)	608, 770, 1000, 1656, 1791, 1915, 2354, 2520
Tru1I	TTAA	8 cut(s)	321, 764, 1557, 1733, 1767, 2057, 2136, 2364
Tru9I	TTAA	8 cut(s)	321, 764, 1557, 1733, 1767, 2057, 2136, 2364
TseFI	GTSAC	8 cut(s)	180, 208, 263, 887, 934, 2011, 2692, 2714
TseI	GCWGC	7 cut(s)	827, 899, 1133, 1481, 1745, 1900, 2566
Tsp45I	GTSAC	8 cut(s)	180, 208, 263, 887, 934, 2011, 2692, 2714
TspDTI	ATGAA	4 cut(s)	826, 1185, 2118, 2431
TspGWI	ACGGA	3 cut(s)	265, 413, 1819
Tth111I	GACNNNGTC	3 cut(s)	1012, 1701, 2540
Van91I	CCANNNNNTGG	1 cut(s)	1498
Vha464I	CTTAAG	1 cut(s)	2363
VpaK11BI	GGWCC	3 cut(s)	273, 1697, 2672
VspI	ATTAAT	2 cut(s)	1557, 2136
XapI	RAATTY	4 cut(s)	284, 1149, 2001, 2438
XbaI	TCTAGA	3 cut(s)	1409, 1652, 1841
XceI	RCATGY	1 cut(s)	1907
XcmI	CCANNNNNNNNNTGG	3 cut(s)	145, 287, 1681
XmnI	GAANNNNTTC	1 cut(s)	1121
XspI	CTAG	9 cut(s)	843, 1338, 1410, 1653, 1724, 1842, 1962, 2349, 2640
ZrmI	AGTACT	1 cut(s)	1507
Zsp2I	ATGCAT	1 cut(s)	235

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.