Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Reverse (-)

67532670 .. 67542035

Gene Structure 9366 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00216900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGCGTCGCTCTCGTCGTCGGCGATCTCCAATCACAGCCTCTCCCCCAATGCCTTTGTGCCTAAGAAGATCTGCTTCTTCTCTAATCAATGCCGCGCATTACTTCTCCCTCAGCGCTCTCCTATTTGCAGGATGGGCTTTGTCTCTGGGCTGGAAAGGAAAAAGACTTCTAAAACCAACATCTTTGCATCAGTCACAACAGACACTTCAGTAGGAACATCCCCTGAGAAAGTCCAGCTTCCAAAAGGTGACAGTTGGTCTGTTCATAAATTTGGGGGCACTTGCGTGGGAAGTTCGGAAAGAATTAATAATGTTGCAAAGATAATTCTTAGTGATGAGTCGGAGAGGAAGTTTGTAGTTGTCTCTGCAATGTCTAAGGTAACAGATATGATGTACGATCTCATCTTAAAGGCTCAATCACGAGACGAAACCTATTTGGCTGCATTAGATGCTGTTTTAGTAAAACACAGATCAACAGCTGTCGATTTACTTGATGGGAATGAACTTGGTAGCTTCTTATCAGCATTAAATCAGGACATCAGTAACCTGAAAGCTATGCTTCGTGCAATATATATTGCTGGTCATGCAACAGAATCTTTCACAGATTTTGTGGTAGGACATGGAGAGTTATGGTCTGCTCAGTTGCTGGCATCTGTTGTTAGAAAGAATGGAGTGGATTGCAAGTGGATGGATACAAGGGAAGTCCTTATTGTTAACCCTACTAGCTCTGATCAAGTCGATCCTGATTTCGAGAAATCTGAAAAGAGACTTGAGAAATGGTACTCAAGAAATCCATCAAAAACAATCATAGCTACTGGTTTTATTGCTAGTACACCCCAAGACATTCCTACTACTTTGAAGAGAGATGGAAGTGACTTCTCTGCAGCTATCATGGGTGCTCTATTCAAGGCTGGTCAAGTTACAATTTGGACAGATGTTGATGGTGTCTATAGTGCGGACCCCAGGAAAGTTAGTGAAGCAGTGATTCTGAAGACATTATCTTATCAAGAGGCCTGGGAAATGTCTTATTTTGGGGCAAATGTTCTGCATCCACGTACCATCATTCCAGTAATGCGATATGACATCCCAATTATCATAAGGAACGTTTTCAACCTCTCTGCACCTGGAACAAAAATTTGCCGCGCTTCTACTGAGGATGAAGTGGACCAGAGTTTGGAATCCTTCGTCAGAGGGTTTGCGACAATAGACAACTTAGCCCTTGTAAATGTTGAAGGAACTGGAATGGCTGGTGTTCCTGGTACAGCTAGTGCTATATTTTCTGCCGTTAAAGATGTGGGGGCTAATGTTATTATGATATCTCAGGCTAGTAGCGAACATTCTGTATGCTTTGCTGTCCCGGAGAAGGAAGTAAAAGCTGTTTCTAAGGCACTGCAATCTAGGTTCCGTGAAGCTTTGAATGCTGGGCGTCTTTCTCAGGTTCAAGTCATTCCAAACTGTAGTATATTGGCAGCGGTTGGCCAGAAAATGGCAAGTACTCCTGGAGTTAGTGCAACTCTTTTCAACGCCCTGGCAAAGGCAAATATTAACGTGCGTGCTATAGCTCAAGGTTGTTCTGAGTACAACATTACAGTAGTATTAAAGCGGGAGGATTGTATTAGGGCCCTGAGAGCGGTTCACTCTAGATTTTATCTTTCAAGAACCACTATAGCAATGGGCATTGTTGGACCAGGACTTATTGGTGCTACATTACTTGATCAACTGAGAGATCAGGCTGCAACTCTTAAGGAAGAGTTTAACATTGACTTGCGTGTAATGGGAATTACTGGCTCAAGGACAATGCTTTTGAGTGAGGCGGGTGTTGACTTATCTAGATGGAGAGAACTTCAACAAGAAAAAGGAGAACGGGCAGATATGGAAAAATTTGTGCAGCATGTACATGGGAATCATTTTATTCCAAATACTGTTCTAGTTGACTGCACAGCTGACTCTGTCATTGCAAGCCATTACTATGGCTGGTTGCGGAAAGGAATACATGTAGTCACCCCCAACAAAAAGGCGAATTCAGGGCCTCTTGATCAGTATTTGAAATTAAGAGCTCTTCAACGGCAATCATATACACATTACTTCTATGAAGCCACTGTTGGAGCTGGCCTCCCAATTATCAATACATTACAAGGGCTCCTAGAAACAGGGGACAAAATATTGCGTATTGAGGGCATCTTCAGTGGGACTTTGAGTTATATCTTCAACAACTTAGTAGGAAGACGAACCTTTAGTGAGGTGGTGGCTGAGGCAAAGCAGGCAGGGTTTACTGAGCCAGACCCAAGGGACGATCTATCTGGAACAGATGTTTGTAGGAAGGTAATAATTCTTGCAAGGGAATCTGGTCTTAAGCTGGAACTCTCTGACATCCCTGTTGAAAGCCTCGTGCCAGAACCATTGAAGGATAGTGCATCAGCTGAGGAATTCATGCAGAAACTTCCACAATTCGATCATGACTTGGCTAAGAAAAGACAGATCGCCGAGGATGCAGGGGAAGTCCTGAGATACGTTGGGGTGGTTGATGTACTCAACCAGAAGGGGGTAGTGAAGCTGCAAGCATACAAAAACGATCACCCATTTGCTCAATTATCAGGGGCAGATAATATCATTGCTTTCACAACAACAAGGTACAAAGATCAGCCGCTAATAGTCCGCGGACCAGGAGCTGGTGCTGAAGTTACAGCTGGTGGAGTCTTCAGTGACATACTGAGGCTTGCCTCATATCTTGGTGCCCCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

914

Amino Acids

99.71

Weight (kDa)

7.56

Isoelectric Point (pI)

34.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	87 - 368	3e-43	Amino acid kinase family
ACT_7	PF13840	403 - 465	5.6e-06	ACT domain
ACT_9	PF22468	408 - 468	4.7e-18	ACT domain
ACT_7	PF13840	483 - 547	2.4e-09	ACT domain
ACT_9	PF22468	490 - 549	1.4e-21	ACT domain
ACT	PF01842	496 - 536	4e-06	ACT domain
NAD_binding_3	PF03447	562 - 698	2e-21	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	706 - 904	4.1e-52	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2732
AccB7I	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
AccBSI	CCGCTC	1 cut(s)	1631
AccII	CGCG	3 cut(s)	96, 1143, 2658
AclI	AACGTT	1 cut(s)	1104
AclWI	GGATC	1 cut(s)	734
AcoI	YGGCCR	1 cut(s)	1477
AcsI	RAATTY	5 cut(s)	269, 1134, 1880, 2020, 2426
AcuI	CTGAAG	5 cut(s)	192, 1010, 2167, 2683, 2697
AcyI	GRCGYC	2 cut(s)	5, 1426
AfeI	AGCGCT	1 cut(s)	116
AfiI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
AflII	CTTAAG	2 cut(s)	1742, 2351
AflIII	ACRYGT	1 cut(s)	1993
AhdI	GACNNNNNGTC	1 cut(s)	256
AjnI	CCWGG	8 cut(s)	962, 1013, 1123, 1255, 1498, 1527, 1687, 2662
AjuI	GAANNNNNNNTTGG	2 cut(s)	2085, 2117
AleI	CACNNNNGTG	1 cut(s)	284
Alw21I	GWGCWC	2 cut(s)	901, 2059
Alw26I	GTCTC	4 cut(s)	148, 367, 417, 760
AlwI	GGATC	1 cut(s)	734
AlwNI	CAGNNNCTG	2 cut(s)	646, 2669
Aor51HI	AGCGCT	1 cut(s)	116
AoxI	GGCC	5 cut(s)	1011, 1477, 1620, 2027, 2110
ApaI	GGGCCC	1 cut(s)	1624
ApeKI	GCWGC	6 cut(s)	440, 884, 1469, 1733, 1888, 2554
ApoI	RAATTY	5 cut(s)	269, 1134, 1880, 2020, 2426
ArsI	GACNNNNNNTTYG	2 cut(s)	1786, 1818
AseI	ATTAAT	1 cut(s)	306
Asp700I	GAANNNNTTC	1 cut(s)	1106
AspLEI	GCGC	3 cut(s)	98, 117, 1145
AspS9I	GGNCC	7 cut(s)	958, 1165, 1620, 1621, 1685, 2027, 2660
AsuC2I	CCSGG	1 cut(s)	1358
AsuHPI	GGTGA	3 cut(s)	260, 1993, 2567
AvaII	GGWCC	4 cut(s)	958, 1165, 1685, 2660
BaeGI	GKGCMC	3 cut(s)	281, 1624, 2737
BalI	TGGCCA	1 cut(s)	1479
BanI	GGYRCC	1 cut(s)	2732
BanII	GRGCYC	3 cut(s)	1624, 2059, 2142
BauI	CACGAG	2 cut(s)	420, 2387
BbsI	GAAGAC	3 cut(s)	998, 2230, 2689
Bbv12I	GWGCWC	2 cut(s)	901, 2059
BbvCI	CCTCAGC	3 cut(s)	111, 2250, 2421
BbvI	GCAGC	6 cut(s)	427, 896, 1481, 1720, 1900, 2541
BccI	CCATC	8 cut(s)	127, 488, 682, 802, 860, 935, 1067, 1827
BceAI	ACGGC	2 cut(s)	1268, 2081
BciT130I	CCWGG	8 cut(s)	964, 1015, 1125, 1257, 1500, 1529, 1689, 2664
BciVI	GTATCC	1 cut(s)	685
BclI	TGATCA	3 cut(s)	730, 1714, 2035
BcnI	CCSGG	1 cut(s)	1358
BcoDI	GTCTC	4 cut(s)	148, 367, 417, 760
BfaI	CTAG	9 cut(s)	723, 828, 1266, 1326, 1398, 1641, 1830, 1928, 2144
BfmI	CTRYAG	5 cut(s)	882, 949, 1456, 1557, 1665
BfoI	RGCGCY	1 cut(s)	118
BfrI	CTTAAG	2 cut(s)	1742, 2351
BfuI	GTATCC	1 cut(s)	685
BglII	AGATCT	1 cut(s)	69
BisI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1470, 1734, 1889, 2555, 2645
BlsI	GCNGC	9 cut(s)	95, 442, 886, 1142, 1471, 1735, 1890, 2556, 2646
BmcAI	AGTACT	1 cut(s)	1495
Bme1390I	CCNGG	9 cut(s)	964, 1015, 1125, 1257, 1358, 1500, 1529, 1689, 2664
Bme18I	GGWCC	4 cut(s)	958, 1165, 1685, 2660
BmeRI	GACNNNNNGTC	1 cut(s)	256
BmgT120I	GGNCC	7 cut(s)	958, 1165, 1620, 1621, 1685, 2027, 2660
BmiI	GGNNCC	5 cut(s)	960, 1403, 1622, 2141, 2734
BmrFI	CCNGG	9 cut(s)	964, 1015, 1125, 1257, 1358, 1500, 1529, 1689, 2664
BmsI	GCATC	7 cut(s)	197, 439, 659, 1057, 2187, 2423, 2479
BpiI	GAAGAC	3 cut(s)	998, 2230, 2689
BplI	GAGNNNNNCTC	2 cut(s)	2097, 2129
BpmI	CTGGAG	1 cut(s)	1521
Bpu10I	CCTNAGC	3 cut(s)	111, 2250, 2421
BpuEI	CTTGAG	4 cut(s)	769, 791, 1548, 1774
BpuMI	CCSGG	1 cut(s)	1358
BsaAI	YACGTR	1 cut(s)	1055
BsaHI	GRCGYC	2 cut(s)	5, 1426
BsaJI	CCNNGG	6 cut(s)	962, 1014, 1527, 2285, 2484, 2656
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 335, 365, 2124, 2154
Bsc4I	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
Bse1I	ACTGG	4 cut(s)	820, 1067, 1243, 1789
Bse3DI	GCAATG	4 cut(s)	375, 1677, 1953, 2610
BseBI	CCWGG	8 cut(s)	964, 1015, 1125, 1257, 1500, 1529, 1689, 2664
BseDI	CCNNGG	6 cut(s)	962, 1014, 1527, 2285, 2484, 2656
BseGI	GGATG	8 cut(s)	138, 218, 693, 1048, 1083, 1162, 2370, 2494
BseLI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
BseMI	GCAATG	4 cut(s)	375, 1677, 1953, 2610
BseNI	ACTGG	4 cut(s)	820, 1067, 1243, 1789
BseSI	GKGCMC	3 cut(s)	281, 1624, 2737
BseXI	GCAGC	6 cut(s)	427, 896, 1481, 1720, 1900, 2541
BseYI	CCCAGC	1 cut(s)	1421
BsgI	GTGCAG	3 cut(s)	1104, 1907, 1921
Bsh1236I	CGCG	3 cut(s)	96, 1143, 2658
BshFI	GGCC	5 cut(s)	1013, 1479, 1622, 2029, 2112
BshNI	GGYRCC	1 cut(s)	2732
BsiHKAI	GWGCWC	2 cut(s)	901, 2059
BsiSI	CCGG	1 cut(s)	1358
BslFI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
BslI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
BsmAI	GTCTC	4 cut(s)	148, 367, 417, 760
BsmBI	CGTCTC	1 cut(s)	417
BsmFI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
BsmI	GAATGC	1 cut(s)	1423
BsnI	GGCC	5 cut(s)	1013, 1479, 1622, 2029, 2112
Bsp120I	GGGCCC	1 cut(s)	1620
Bsp1286I	GDGCHC	6 cut(s)	281, 901, 1624, 2059, 2142, 2737
Bsp1407I	TGTACA	1 cut(s)	1894
BspANI	GGCC	5 cut(s)	1013, 1479, 1622, 2029, 2112
BspFNI	CGCG	3 cut(s)	96, 1143, 2658
BspHI	TCATGA	1 cut(s)	2455
BspLI	GGNNCC	5 cut(s)	960, 1403, 1622, 2141, 2734
BspMAI	CTGCAG	1 cut(s)	886
BspPI	GGATC	1 cut(s)	734
BspQI	GCTCTTC	1 cut(s)	2064
BspT107I	GGYRCC	1 cut(s)	2732
BspTI	CTTAAG	2 cut(s)	1742, 2351
BsrBI	CCGCTC	1 cut(s)	1631
BsrDI	GCAATG	4 cut(s)	375, 1677, 1953, 2610
BsrGI	TGTACA	1 cut(s)	1894
BsrI	ACTGG	4 cut(s)	820, 1067, 1243, 1789
BssECI	CCNNGG	6 cut(s)	962, 1014, 1527, 2285, 2484, 2656
BssNI	GRCGYC	2 cut(s)	5, 1426
BssSI	CACGAG	2 cut(s)	420, 2387
BssT1I	CCWWGG	1 cut(s)	2285
Bst2BI	CACGAG	2 cut(s)	420, 2387
Bst2UI	CCWGG	8 cut(s)	964, 1015, 1125, 1257, 1500, 1529, 1689, 2664
Bst4CI	ACNGT	5 cut(s)	254, 1457, 1591, 1924, 2101
Bst6I	CTCTTC	3 cut(s)	854, 1743, 2064
BstACI	GRCGYC	2 cut(s)	5, 1426
BstAFI	CTTAAG	2 cut(s)	1742, 2351
BstAPI	GCANNNNNTGC	1 cut(s)	449
BstAUI	TGTACA	1 cut(s)	1894
BstBAI	YACGTR	1 cut(s)	1055
BstC8I	GCNNGC	7 cut(s)	648, 1554, 1960, 2110, 2262, 2559, 2718
BstDSI	CCRYGG	1 cut(s)	2656
BstENI	CCTNNNNNAGG	1 cut(s)	1532
BstF5I	GGATG	8 cut(s)	138, 218, 693, 1048, 1083, 1162, 2370, 2494
BstFNI	CGCG	3 cut(s)	96, 1143, 2658
BstH2I	RGCGCY	1 cut(s)	118
BstHHI	GCGC	3 cut(s)	98, 117, 1145
BstMAI	GTCTC	4 cut(s)	148, 367, 417, 760
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1418, 1628, 2261, 2489
BstNI	CCWGG	8 cut(s)	964, 1015, 1125, 1257, 1500, 1529, 1689, 2664
BstNSI	RCATGY	2 cut(s)	1895, 1997
BstSCI	CCNGG	9 cut(s)	962, 1013, 1123, 1255, 1356, 1498, 1527, 1687, 2662
BstSFI	CTRYAG	5 cut(s)	882, 949, 1456, 1557, 1665
BstSLI	GKGCMC	3 cut(s)	281, 1624, 2737
BstUI	CGCG	3 cut(s)	96, 1143, 2658
BstV1I	GCAGC	6 cut(s)	427, 896, 1481, 1720, 1900, 2541
BstV2I	GAAGAC	3 cut(s)	998, 2230, 2689
BstX2I	RGATCY	1 cut(s)	69
BstXI	CCANNNNNNTGG	1 cut(s)	1970
BstYI	RGATCY	1 cut(s)	69
BsuI	GTATCC	1 cut(s)	685
BsuRI	GGCC	5 cut(s)	1013, 1479, 1622, 2029, 2112
BtgI	CCRYGG	1 cut(s)	2656
BtsCI	GGATG	8 cut(s)	138, 218, 693, 1048, 1083, 1162, 2370, 2494
BtsI	GCAGTG	2 cut(s)	987, 1388
BtsIMutI	CAGTG	5 cut(s)	987, 1388, 2097, 2191, 2707
Cac8I	GCNNGC	7 cut(s)	648, 1554, 1960, 2110, 2262, 2559, 2718
CaiI	CAGNNNCTG	2 cut(s)	646, 2669
CciI	TCATGA	1 cut(s)	2455
CfoI	GCGC	3 cut(s)	98, 117, 1145
Cfr13I	GGNCC	7 cut(s)	958, 1165, 1620, 1621, 1685, 2027, 2660
Cfr42I	CCGCGG	1 cut(s)	2659
CseI	GACGC	1 cut(s)	1415
CviAII	CATG	8 cut(s)	584, 620, 892, 1892, 1898, 1994, 2431, 2456
DriI	GACNNNNNGTC	1 cut(s)	256
EaeI	YGGCCR	1 cut(s)	1477
Eam1104I	CTCTTC	3 cut(s)	854, 1743, 2064
Eam1105I	GACNNNNNGTC	1 cut(s)	256
EarI	CTCTTC	3 cut(s)	854, 1743, 2064
Ecl136II	GAGCTC	1 cut(s)	2057
Eco130I	CCWWGG	1 cut(s)	2285
Eco147I	AGGCCT	1 cut(s)	1013
Eco24I	GRGCYC	3 cut(s)	1624, 2059, 2142
Eco32I	GATATC	1 cut(s)	1317
Eco47I	GGWCC	4 cut(s)	958, 1165, 1685, 2660
Eco47III	AGCGCT	1 cut(s)	116
Eco53kI	GAGCTC	1 cut(s)	2057
Eco57I	CTGAAG	5 cut(s)	192, 1010, 2167, 2683, 2697
EcoICRI	GAGCTC	1 cut(s)	2057
EcoNI	CCTNNNNNAGG	1 cut(s)	1532
EcoO109I	RGGNCCY	3 cut(s)	1620, 1621, 2027
EcoRI	GAATTC	2 cut(s)	2020, 2426
EcoRII	CCWGG	8 cut(s)	962, 1013, 1123, 1255, 1498, 1527, 1687, 2662
EcoRV	GATATC	1 cut(s)	1317
EcoT14I	CCWWGG	1 cut(s)	2285
EcoT38I	GRGCYC	3 cut(s)	1624, 2059, 2142
ErhI	CCWWGG	1 cut(s)	2285
Esp3I	CGTCTC	1 cut(s)	417
FaeI	CATG	8 cut(s)	587, 623, 895, 1895, 1901, 1997, 2434, 2459
FalI	AAGNNNNNCTT	6 cut(s)	59, 91, 222, 254, 543, 575
FaqI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
FatI	CATG	8 cut(s)	583, 619, 891, 1891, 1897, 1993, 2430, 2455
FauI	CCCGC	2 cut(s)	1596, 1807
FbaI	TGATCA	3 cut(s)	730, 1714, 2035
Fnu4HI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1470, 1734, 1889, 2555, 2645
FokI	GGATG	8 cut(s)	145, 205, 700, 1035, 1070, 1169, 2357, 2501
FriOI	GRGCYC	3 cut(s)	1624, 2059, 2142
Fsp4HI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1470, 1734, 1889, 2555, 2645
FspBI	CTAG	9 cut(s)	723, 828, 1266, 1326, 1398, 1641, 1830, 1928, 2144
GlaI	GCGC	3 cut(s)	97, 116, 1144
GluI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1470, 1734, 1889, 2555, 2645
GsaI	CCCAGC	1 cut(s)	1425
GsuI	CTGGAG	1 cut(s)	1521
HaeII	RGCGCY	1 cut(s)	118
HaeIII	GGCC	5 cut(s)	1013, 1479, 1622, 2029, 2112
HapII	CCGG	1 cut(s)	1358
HgaI	GACGC	1 cut(s)	1415
HhaI	GCGC	3 cut(s)	98, 117, 1145
Hin1I	GRCGYC	2 cut(s)	5, 1426
Hin1II	CATG	8 cut(s)	587, 623, 895, 1895, 1901, 1997, 2434, 2459
Hin6I	GCGC	3 cut(s)	96, 115, 1143
HinP1I	GCGC	3 cut(s)	96, 115, 1143
HincII	GTYRAC	3 cut(s)	715, 1822, 1933
HindII	GTYRAC	3 cut(s)	715, 1822, 1933
HindIII	AAGCTT	1 cut(s)	1410
HinfI	GANTC	8 cut(s)	338, 593, 985, 1178, 1903, 1946, 2342, 2694
HpaI	GTTAAC	1 cut(s)	715
HpaII	CCGG	1 cut(s)	1358
HphI	GGTGA	3 cut(s)	260, 1993, 2567
Hpy166II	GTNNAC	7 cut(s)	715, 833, 1165, 1636, 1822, 1933, 2271
Hpy188I	TCNGA	8 cut(s)	298, 343, 730, 760, 990, 1190, 1576, 2368
Hpy8I	GTNNAC	7 cut(s)	715, 833, 1165, 1636, 1822, 1933, 2271
Hpy99I	CGWCG	3 cut(s)	10, 19, 22
HpyAV	CCTTC	6 cut(s)	1192, 1226, 1357, 2314, 2398, 2533
HpyCH4III	ACNGT	5 cut(s)	254, 1457, 1591, 1924, 2101
HpyCH4IV	ACGT	4 cut(s)	1054, 1104, 1548, 2511
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1418, 1628, 2261, 2489
HpySE526I	ACGT	4 cut(s)	1054, 1104, 1548, 2511
Hsp92I	GRCGYC	2 cut(s)	5, 1426
Hsp92II	CATG	8 cut(s)	587, 623, 895, 1895, 1901, 1997, 2434, 2459
HspAI	GCGC	3 cut(s)	96, 115, 1143
Ksp22I	TGATCA	3 cut(s)	730, 1714, 2035
KspAI	GTTAAC	1 cut(s)	715
KspI	CCGCGG	1 cut(s)	2659
LguI	GCTCTTC	1 cut(s)	2064
LmnI	GCTCC	3 cut(s)	2105, 2145, 2666
Lsp1109I	GCAGC	6 cut(s)	427, 896, 1481, 1720, 1900, 2541
LweI	GCATC	7 cut(s)	197, 439, 659, 1057, 2187, 2423, 2479
MaeI	CTAG	9 cut(s)	723, 828, 1266, 1326, 1398, 1641, 1830, 1928, 2144
MaeII	ACGT	4 cut(s)	1054, 1104, 1548, 2511
MaeIII	GTNAC	9 cut(s)	193, 248, 379, 542, 872, 919, 1999, 2680, 2702
MbiI	CCGCTC	1 cut(s)	1631
MflI	RGATCY	1 cut(s)	69
MhlI	GDGCHC	6 cut(s)	281, 901, 1624, 2059, 2142, 2737
MlsI	TGGCCA	1 cut(s)	1479
MluNI	TGGCCA	1 cut(s)	1479
MlyI	GAGTC	3 cut(s)	347, 1940, 2703
MmeI	TCCRAC	3 cut(s)	321, 1663, 2083
Mox20I	TGGCCA	1 cut(s)	1479
MroXI	GAANNNNTTC	1 cut(s)	1106
MscI	TGGCCA	1 cut(s)	1479
MslI	CAYNNNNRTG	3 cut(s)	284, 1896, 1968
Msp20I	TGGCCA	1 cut(s)	1479
MspA1I	CMGCKG	6 cut(s)	479, 1472, 1943, 2420, 2658, 2687
MspCI	CTTAAG	2 cut(s)	1742, 2351
MspI	CCGG	1 cut(s)	1358
MspR9I	CCNGG	9 cut(s)	964, 1015, 1125, 1257, 1358, 1500, 1529, 1689, 2664
Mva1269I	GAATGC	1 cut(s)	1423
MvaI	CCWGG	8 cut(s)	964, 1015, 1125, 1257, 1500, 1529, 1689, 2664
MvnI	CGCG	3 cut(s)	96, 1143, 2658
MwoI	GCNNNNNNNGC	7 cut(s)	135, 449, 584, 1418, 1628, 2261, 2489
NciI	CCSGG	1 cut(s)	1358
NlaIII	CATG	8 cut(s)	587, 623, 895, 1895, 1901, 1997, 2434, 2459
NlaIV	GGNNCC	5 cut(s)	960, 1403, 1622, 2141, 2734
NmeAIII	GCCGAG	1 cut(s)	2509
NmuCI	GTSAC	5 cut(s)	193, 248, 872, 1999, 2702
NspI	RCATGY	2 cut(s)	1895, 1997
OliI	CACNNNNGTG	1 cut(s)	284
PagI	TCATGA	1 cut(s)	2455
PceI	AGGCCT	1 cut(s)	1013
PciI	ACATGT	1 cut(s)	1993
PciSI	GCTCTTC	1 cut(s)	2064
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 20
PctI	GAATGC	1 cut(s)	1423
PdmI	GAANNNNTTC	1 cut(s)	1106
PfeI	GAWTC	5 cut(s)	593, 985, 1178, 1903, 2342
PflFI	GACNNNGTC	1 cut(s)	1949
PflMI	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
PfoI	TCCNGGA	2 cut(s)	1356, 1498
PkrI	GCNGC	9 cut(s)	95, 442, 886, 1142, 1471, 1735, 1890, 2556, 2646
PleI	GAGTC	3 cut(s)	346, 1940, 2702
PpsI	GAGTC	3 cut(s)	346, 1940, 2702
Ppu21I	YACGTR	1 cut(s)	1055
PscI	ACATGT	1 cut(s)	1993
PshBI	ATTAAT	1 cut(s)	306
Psp124BI	GAGCTC	1 cut(s)	2059
Psp1406I	AACGTT	1 cut(s)	1104
Psp6I	CCWGG	8 cut(s)	962, 1013, 1123, 1255, 1498, 1527, 1687, 2662
PspFI	CCCAGC	1 cut(s)	1421
PspGI	CCWGG	8 cut(s)	962, 1013, 1123, 1255, 1498, 1527, 1687, 2662
PspN4I	GGNNCC	5 cut(s)	960, 1403, 1622, 2141, 2734
PspOMI	GGGCCC	1 cut(s)	1620
PspPI	GGNCC	7 cut(s)	958, 1165, 1620, 1621, 1685, 2027, 2660
PstI	CTGCAG	1 cut(s)	886
PstNI	CAGNNNCTG	2 cut(s)	646, 2669
PsuI	RGATCY	1 cut(s)	69
PsyI	GACNNNGTC	1 cut(s)	1949
PvuII	CAGCTG	4 cut(s)	479, 1943, 2420, 2687
RseI	CAYNNNNRTG	3 cut(s)	284, 1896, 1968
SacI	GAGCTC	1 cut(s)	2059
SacII	CCGCGG	1 cut(s)	2659
SapI	GCTCTTC	1 cut(s)	2064
SatI	GCNGC	9 cut(s)	94, 441, 885, 1141, 1470, 1734, 1889, 2555, 2645
Sau96I	GGNCC	7 cut(s)	958, 1165, 1620, 1621, 1685, 2027, 2660
ScaI	AGTACT	1 cut(s)	1495
SchI	GAGTC	3 cut(s)	347, 1940, 2703
ScrFI	CCNGG	9 cut(s)	964, 1015, 1125, 1257, 1358, 1500, 1529, 1689, 2664
SduI	GDGCHC	6 cut(s)	281, 901, 1624, 2059, 2142, 2737
SfaNI	GCATC	7 cut(s)	197, 439, 659, 1057, 2187, 2423, 2479
SfcI	CTRYAG	5 cut(s)	882, 949, 1456, 1557, 1665
Sfr303I	CCGCGG	1 cut(s)	2659
SgrBI	CCGCGG	1 cut(s)	2659
SinI	GGWCC	4 cut(s)	958, 1165, 1685, 2660
SmiMI	CAYNNNNRTG	3 cut(s)	284, 1896, 1968
SmlI	CTYRAG	6 cut(s)	770, 784, 1563, 1742, 1789, 2351
SmoI	CTYRAG	6 cut(s)	770, 784, 1563, 1742, 1789, 2351
SseBI	AGGCCT	1 cut(s)	1013
SspI	AATATT	2 cut(s)	1543, 2163
SspMI	CTAG	9 cut(s)	723, 828, 1266, 1326, 1398, 1641, 1830, 1928, 2144
SstI	GAGCTC	1 cut(s)	2059
StuI	AGGCCT	1 cut(s)	1013
StyD4I	CCNGG	9 cut(s)	962, 1013, 1123, 1255, 1356, 1498, 1527, 1687, 2662
StyI	CCWWGG	1 cut(s)	2285
TaaI	ACNGT	5 cut(s)	254, 1457, 1591, 1924, 2101
TaiI	ACGT	4 cut(s)	1057, 1107, 1551, 2514
TaqI	TCGA	4 cut(s)	483, 738, 750, 2451
TatI	WGTACW	5 cut(s)	830, 1493, 1578, 1894, 2527
TauI	GCSGC	3 cut(s)	96, 1143, 2647
TfiI	GAWTC	5 cut(s)	593, 985, 1178, 1903, 2342
TscAI	CASTG	5 cut(s)	987, 1395, 2104, 2191, 2707
TseFI	GTSAC	5 cut(s)	193, 248, 872, 1999, 2702
TseI	GCWGC	6 cut(s)	440, 884, 1469, 1733, 1888, 2554
Tsp45I	GTSAC	5 cut(s)	193, 248, 872, 1999, 2702
TspDTI	ATGAA	5 cut(s)	254, 516, 1173, 2106, 2419
TspGWI	ACGGA	1 cut(s)	1394
TspRI	CASTG	5 cut(s)	987, 1395, 2104, 2191, 2707
Tth111I	GACNNNGTC	1 cut(s)	1949
Van91I	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
Vha464I	CTTAAG	2 cut(s)	1742, 2351
VpaK11BI	GGWCC	4 cut(s)	958, 1165, 1685, 2660
VspI	ATTAAT	1 cut(s)	306
XagI	CCTNNNNNAGG	1 cut(s)	1532
XapI	RAATTY	5 cut(s)	269, 1134, 1880, 2020, 2426
XbaI	TCTAGA	2 cut(s)	1640, 1829
XceI	RCATGY	2 cut(s)	1895, 1997
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1669
XmnI	GAANNNNTTC	1 cut(s)	1106
XspI	CTAG	9 cut(s)	723, 828, 1266, 1326, 1398, 1641, 1830, 1928, 2144
ZrmI	AGTACT	1 cut(s)	1495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.