Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Forward (+)

761339 .. 770525

Gene Structure 9187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1004700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGCGTCCCTCTCTTCGGCGATCTCCAGTAATCCCAGCCACACGCTCTTCCCCAACGCAATGGCTCAAGACGCCAAGCCCAGGAAGCGCTGCTACTCTCATTGCCCTACATTTCTTCTCCCTCCTCACCGCTCTCCCATTTGCAGAAGGGGTTTTGTTTCGGGGCTGGAAAGGAAAAGGACACTGAAAACCCGTATCTTTGCATCACTCACAGATACTCCAGTAAATGTATCCCCTGAAAAAGTTCAGCTTCCGAAAGGTGACAGCTGGTCTGTTCATAAATTTGGCGGTACTTGTGTGGGGAGTTCAGAAAGAATTAAGAATGTTGCAAAAATAATTCTTAGTGATGATTCAGAGAGGAAATTTGTAGTTGTCTCTGCAATGTCCAAGGTAACAGATATGATGTATGATCTTATCTACAAGGCTCAATCACGAGATGAGGCCTACATATCTGCATTAAATGTGGTTTTAGAAAAGCACAAATGTACAGCTCTTGATCTACTTGATGGAGATGATCTTAGTAGTTTCTTAGCACAATTGCATCACGACATCAGTAACCTGAAAGCGATGCTTCGTGCTATATACATTGCTGGTCATGCGACAGAGTCATTCACAGAATTTGTTGTAGGACATGGAGAGTTATGGTCTGTGCAGATGCTGTCATGTGTTATTAGAAAGAATGGGGTAGATTGCAATTGGATGGATACAAGGGAAGTCCTTATTGTGAATCCTACAAGTTCTAATCAAGTTGATCCTGATTTCAAGGCATCTGAAGATAGACTTGAGAAATGGTATTCCAAAAATCCATCAAAAACAATTGTAGCTACTGGCTTTATTGCTAGCACACCTCAAAATATTCCTACTACTTTGAAGAGAGATGGAAGTGACTTCTCTGCAGCTATCATGGGTGCTCTATTCGAGGCTCGTCAAGTCACAATTTGGACAGATGTTGATGGAGTCTATAGTGCAGATCCCAGAAAAGTCAGTGAGGCAGTGATTCTGAAGACTTTGTCTTATCAAGAAGCCTGGGAAATGTCTTATTTTGGGGCAAATGTTCTGCATCCACGCACTATCATCCCAGTGATGAGATATGACATTCCAATTATAATAAGGAACGTTTTCAACCTTGCTGCACCTGGAACAAAAATTTGTCGCTCTACTGAGGATGAAACTGCCCAGGGTTTGGAGTCGTTTGTCAAAGGGTTTGCCACAATAGACAACTTGGCTCTTGTCAATGTGGAAGGAACTGGAATGGCTGGTGTTCCTGGTACAGCCAGTGCTATCTTTAGTGCCGTAAAAGATGTTGGGGCTAATGTTATTATGATATCTCAGGCTAGTAGTGAGCACTCTGTGTGCTTTGCTGTCCCTGAGAAGGAAGTGAATGCTGTTTCTGAGTTACTGCAGTCTAGATTTCGTGAGGCTTTGAATGTTGGGCGTCTTTCTAAGGTTCAAGTCATTCCAAACTGTAGTATTTTGGCAACGGTTGGCCAAAAAATGGCCAGTACTCCAGGAGTTAGTGCAACTCTTTTCAATGCACTGGCAAAGGCAAACATTAATGTGCGTGCCATTGCTCAAGGTTGTTCTGAGTACAATATTACTGTAGTTGTAAAGCGGGAGGATTGTATTAGGGCTCTGAGAGCTGTCCATTCTAGATTCTATCTTTCAAGAACCACAATAGCAATGGGCATCATCGGACCTGGTCTGATTGGTGCCACATTACTAGATCAATTAAGGGATCAGACAGCTACCCTCAAGGAAGAGTTTAACATTGATTTGCGTGTTATGGGAATCACTGGCTCACAGACAATGCTTTTGAGTGAGGCGGGTGTTGACTTATCTAGATGGAGAGAACTCCAAAAAGAGAAAGGAGTAGTAGCAGATATGGAAAAGTTTGTGCAGCATGTACATGGGAATCATTTTATTCCAAATACTGTTTTGGTTGACTGCACAGCTGACTCTAGTATTGCAAGCCATTATTATGAATGGTTGCGGAAAGGAATTCACGTAGTCACTCCCAACAAAAAGGCAAACTCAGGGCCACTTGATCAATATTTGAAATTACGAGCTCTTCAACGGCAATCATATACACATTACTTCTATGAAGCTACCGTTGGAGCTGGTCTGCCAATTATTAATACATTACAAGGTCTCCTGGAAACTGGAGACAAAATATTGCGCATTGAAGGCATCTTCAGTGGTACTTTGAGTTATATCTTCAACAATTTCACTGGTGGACGCACTTTTAGTGAGGTGGTGGCTGAAGCAAAGCAGGCAGGTTATACTGAGCCGGATCCAAGAGATGATCTTTCTGGAACAGATGTTTGTAGAAAGGTCATAATTCTTGCTAGGGAATCTGGTCTTAAGCTGGAACTCTCTGACATCCCTGTTGAAAGCCTTGTGCCGGAACCCTTGAAGGATAGTGCATCAGCCGAGGAATTCATGCAAAAACTTCCTCAATTCGATCAAGAGATGAGTAAGAAAAGACAAATTGCTGAGGATGCGGGAGGAGTCTTGAGATACGTTGGAGTGGTTGACATGGTCAATCAGAAAGGGGAAGTGAAGCTGCAGAAGTACAAGAACGATCATCCTTTCGCTCAACTGTCTGGGGCAGATAATATAATTGCGTTCACAACAACTAGATACAAAGATCAGCCCCTAATAGTTCGTGGGCCTGGTGCTGGTGCTGAAGTCACTGCTGGCGGTGTCTTCAGTGACCTGTTGCGGCTTGCTTCATACCTTGGTGCACCGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

918

Amino Acids

100.57

Weight (kDa)

7.27

Isoelectric Point (pI)

35.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	91 - 372	1.5e-41	Amino acid kinase family
ACT_9	PF22468	411 - 471	4e-17	ACT domain
ACT_7	PF13840	486 - 550	2.3e-10	ACT domain
ACT_9	PF22468	493 - 552	1.3e-22	ACT domain
ACT	PF01842	498 - 540	1e-06	ACT domain
NAD_binding_3	PF03447	565 - 701	5.3e-22	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	709 - 907	1.5e-53	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1106
Acc16I	TGCGCA	1 cut(s)	2177
Acc36I	ACCTGC	1 cut(s)	2264
AccB1I	GGYRCC	1 cut(s)	1709
AccB7I	CCANNNNNTGG	2 cut(s)	1183, 1495
AccBSI	CCGCTC	1 cut(s)	132
AciI	CCGC	8 cut(s)	130, 288, 1612, 1823, 1990, 2501, 2700, 2722
AclI	AACGTT	1 cut(s)	1116
AclWI	GGATC	5 cut(s)	746, 965, 1743, 2285, 2298
AcoI	YGGCCR	2 cut(s)	1486, 1497
AcsI	RAATTY	6 cut(s)	281, 362, 617, 1146, 1998, 2435
AcuI	CTGAAG	6 cut(s)	792, 1022, 2176, 2280, 2692, 2706
AcyI	GRCGYC	3 cut(s)	5, 72, 1435
AdeI	CACNNNGTG	1 cut(s)	1351
AfaI	GTAC	8 cut(s)	292, 487, 1270, 1504, 1589, 1905, 2200, 2573
AfeI	AGCGCT	1 cut(s)	89
AfiI	CCNNNNNNNGG	7 cut(s)	16, 81, 1183, 1372, 1495, 2401, 2678
AflII	CTTAAG	1 cut(s)	2360
AhdI	GACNNNNNGTC	1 cut(s)	268
AjnI	CCWGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
AjuI	GAANNNNNNNTTGG	2 cut(s)	2094, 2126
Alw21I	GWGCWC	4 cut(s)	913, 1347, 2068, 2746
Alw26I	GTCTC	3 cut(s)	379, 2153, 2157
Alw44I	GTGCAC	1 cut(s)	2742
AlwI	GGATC	5 cut(s)	746, 965, 1743, 2285, 2298
AlwNI	CAGNNNCTG	1 cut(s)	658
Aor51HI	AGCGCT	1 cut(s)	89
AoxI	GGCC	5 cut(s)	441, 1486, 1497, 2036, 2669
ApaLI	GTGCAC	1 cut(s)	2742
ApeKI	GCWGC	5 cut(s)	90, 896, 1130, 1897, 2563
ApoI	RAATTY	6 cut(s)	281, 362, 617, 1146, 1998, 2435
ArsI	GACNNNNNNTTYG	2 cut(s)	1795, 1827
AseI	ATTAAT	2 cut(s)	1554, 2133
Asp700I	GAANNNNTTC	2 cut(s)	243, 1118
AspLEI	GCGC	2 cut(s)	90, 2178
AspS9I	GGNCC	3 cut(s)	1694, 2036, 2669
AsuHPI	GGTGA	2 cut(s)	119, 272
AsuNHI	GCTAGC	1 cut(s)	839
AvaII	GGWCC	1 cut(s)	1694
BaeGI	GKGCMC	1 cut(s)	2746
BaeI	ACNNNNGTAYC	4 cut(s)	207, 240, 282, 315
BalI	TGGCCA	2 cut(s)	1488, 1499
BamHI	GGATCC	1 cut(s)	2290
BanI	GGYRCC	1 cut(s)	1709
BanII	GRGCYC	2 cut(s)	1633, 2068
BarI	GAAGNNNNNNTAC	2 cut(s)	77, 109
BauI	CACGAG	1 cut(s)	432
BbsI	GAAGAC	2 cut(s)	1010, 2698
Bbv12I	GWGCWC	4 cut(s)	913, 1347, 2068, 2746
BbvCI	CCTCAGC	1 cut(s)	2493
BbvI	GCAGC	5 cut(s)	77, 908, 1117, 1909, 2550
BccI	CCATC	6 cut(s)	500, 694, 814, 872, 947, 1836
BceAI	ACGGC	2 cut(s)	1277, 2090
BciT130I	CCWGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
BciVI	GTATCC	2 cut(s)	241, 697
BclI	TGATCA	1 cut(s)	2044
BcoDI	GTCTC	3 cut(s)	379, 2153, 2157
BfaI	CTAG	9 cut(s)	840, 1335, 1407, 1650, 1721, 1839, 1959, 2346, 2637
BfmI	CTRYAG	6 cut(s)	894, 961, 1400, 1465, 1599, 2564
BfoI	RGCGCY	1 cut(s)	91
BfrI	CTTAAG	1 cut(s)	2360
BfuAI	ACCTGC	1 cut(s)	2264
BfuI	GTATCC	2 cut(s)	241, 697
BisI	GCNGC	6 cut(s)	91, 897, 1131, 1898, 2564, 2723
BlsI	GCNGC	6 cut(s)	92, 898, 1132, 1899, 2565, 2724
BmcAI	AGTACT	1 cut(s)	1504
Bme1390I	CCNGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
Bme18I	GGWCC	1 cut(s)	1694
BmeRI	GACNNNNNGTC	1 cut(s)	268
BmgT120I	GGNCC	3 cut(s)	1694, 2036, 2669
BmiI	GGNNCC	3 cut(s)	1711, 2292, 2406
BmrFI	CCNGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
BmrI	ACTGGG	1 cut(s)	1073
BmtI	GCTAGC	1 cut(s)	843
BmuI	ACTGGG	1 cut(s)	1073
BpiI	GAAGAC	2 cut(s)	1010, 2698
BpmI	CTGGAG	4 cut(s)	10, 204, 1491, 2181
Bpu10I	CCTNAGC	1 cut(s)	2493
BpuEI	CTTGAG	5 cut(s)	51, 803, 1557, 1736, 2533
BsaAI	YACGTR	1 cut(s)	2005
BsaHI	GRCGYC	3 cut(s)	5, 72, 1435
BsaI	GGTCTC	1 cut(s)	2153
BsaJI	CCNNGG	7 cut(s)	80, 387, 1026, 1176, 1177, 2430, 2737
BsaXI	ACNNNNNCTCC	4 cut(s)	202, 232, 2133, 2163
Bsc4I	CCNNNNNNNGG	7 cut(s)	16, 81, 1183, 1372, 1495, 2401, 2678
Bse3DI	GCAATG	6 cut(s)	66, 100, 387, 585, 1566, 1686
BseBI	CCWGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
BseDI	CCNNGG	7 cut(s)	80, 387, 1026, 1176, 1177, 2430, 2737
BseGI	GGATG	7 cut(s)	705, 1060, 1074, 1171, 2379, 2503, 2584
BseLI	CCNNNNNNNGG	7 cut(s)	16, 81, 1183, 1372, 1495, 2401, 2678
BseMI	GCAATG	6 cut(s)	66, 100, 387, 585, 1566, 1686
BseMII	CTCAG	9 cut(s)	1152, 1343, 1359, 1383, 1575, 1625, 2046, 2274, 2484
BseRI	GAGGAG	2 cut(s)	114, 2520
BseSI	GKGCMC	1 cut(s)	2746
BseXI	GCAGC	5 cut(s)	77, 908, 1117, 1909, 2550
BseYI	CCCAGC	1 cut(s)	35
BsgI	GTGCAG	5 cut(s)	671, 987, 1116, 1916, 1930
BshFI	GGCC	5 cut(s)	443, 1488, 1499, 2038, 2671
BshNI	GGYRCC	1 cut(s)	1709
BsiHKAI	GWGCWC	4 cut(s)	913, 1347, 2068, 2746
BsiSI	CCGG	2 cut(s)	2288, 2402
BslFI	GGGAC	1 cut(s)	1349
BslI	CCNNNNNNNGG	7 cut(s)	16, 81, 1183, 1372, 1495, 2401, 2678
BsmAI	GTCTC	3 cut(s)	379, 2153, 2157
BsmFI	GGGAC	1 cut(s)	1349
BsmI	GAATGC	1 cut(s)	1387
BsnI	GGCC	5 cut(s)	443, 1488, 1499, 2038, 2671
Bso31I	GGTCTC	1 cut(s)	2153
Bsp1286I	GDGCHC	5 cut(s)	913, 1347, 1633, 2068, 2746
Bsp1407I	TGTACA	2 cut(s)	485, 1903
BspACI	CCGC	8 cut(s)	130, 288, 1612, 1823, 1990, 2501, 2700, 2722
BspANI	GGCC	5 cut(s)	443, 1488, 1499, 2038, 2671
BspCNI	CTCAG	9 cut(s)	1153, 1342, 1360, 1384, 1576, 1626, 2045, 2275, 2485
BspLI	GGNNCC	3 cut(s)	1711, 2292, 2406
BspMAI	CTGCAG	3 cut(s)	898, 1404, 2568
BspMI	ACCTGC	1 cut(s)	2264
BspOI	GCTAGC	1 cut(s)	843
BspPI	GGATC	5 cut(s)	746, 965, 1743, 2285, 2298
BspQI	GCTCTTC	2 cut(s)	53, 2073
BspT107I	GGYRCC	1 cut(s)	1709
BspTI	CTTAAG	1 cut(s)	2360
BspTNI	GGTCTC	1 cut(s)	2153
BsrBI	CCGCTC	1 cut(s)	132
BsrDI	GCAATG	6 cut(s)	66, 100, 387, 585, 1566, 1686
BsrGI	TGTACA	2 cut(s)	485, 1903
BssECI	CCNNGG	7 cut(s)	80, 387, 1026, 1176, 1177, 2430, 2737
BssNI	GRCGYC	3 cut(s)	5, 72, 1435
BssSI	CACGAG	1 cut(s)	432
BssT1I	CCWWGG	2 cut(s)	387, 2737
Bst2BI	CACGAG	1 cut(s)	432
Bst2UI	CCWGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
Bst4CI	ACNGT	7 cut(s)	1466, 1483, 1600, 1933, 2110, 2601, 2748
Bst6I	CTCTTC	5 cut(s)	19, 53, 866, 1752, 2073
BstACI	GRCGYC	3 cut(s)	5, 72, 1435
BstAFI	CTTAAG	1 cut(s)	2360
BstAUI	TGTACA	2 cut(s)	485, 1903
BstBAI	YACGTR	1 cut(s)	2005
BstC8I	GCNNGC	6 cut(s)	841, 1563, 1969, 2271, 2698, 2727
BstF5I	GGATG	7 cut(s)	705, 1060, 1074, 1171, 2379, 2503, 2584
BstH2I	RGCGCY	1 cut(s)	91
BstHHI	GCGC	2 cut(s)	90, 2178
BstMAI	GTCTC	3 cut(s)	379, 2153, 2157
BstMWI	GCNNNNNNNGC	7 cut(s)	71, 85, 596, 1637, 2184, 2270, 2498
BstNI	CCWGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
BstNSI	RCATGY	1 cut(s)	1904
BstSCI	CCNGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
BstSFI	CTRYAG	6 cut(s)	894, 961, 1400, 1465, 1599, 2564
BstSLI	GKGCMC	1 cut(s)	2746
BstV1I	GCAGC	5 cut(s)	77, 908, 1117, 1909, 2550
BstV2I	GAAGAC	2 cut(s)	1010, 2698
BstX2I	RGATCY	2 cut(s)	970, 2290
BstXI	CCANNNNNNTGG	1 cut(s)	61
BstYI	RGATCY	2 cut(s)	970, 2290
BsuI	GTATCC	2 cut(s)	241, 697
BsuRI	GGCC	5 cut(s)	443, 1488, 1499, 2038, 2671
BtgZI	GCGATG	1 cut(s)	581
BtsCI	GGATG	7 cut(s)	705, 1060, 1074, 1171, 2379, 2503, 2584
BtsI	GCAGTG	2 cut(s)	999, 2691
BveI	ACCTGC	1 cut(s)	2264
Cac8I	GCNNGC	6 cut(s)	841, 1563, 1969, 2271, 2698, 2727
CaiI	CAGNNNCTG	1 cut(s)	658
CfoI	GCGC	2 cut(s)	90, 2178
Cfr13I	GGNCC	3 cut(s)	1694, 2036, 2669
CseI	GACGC	3 cut(s)	80, 1424, 2244
CsiI	ACCWGGT	1 cut(s)	1696
Csp6I	GTAC	8 cut(s)	291, 486, 1269, 1503, 1588, 1904, 2199, 2572
CviAII	CATG	8 cut(s)	596, 632, 663, 904, 1901, 1907, 2440, 2536
CviQI	GTAC	8 cut(s)	291, 486, 1269, 1503, 1588, 1904, 2199, 2572
DraIII	CACNNNGTG	1 cut(s)	1351
DriI	GACNNNNNGTC	1 cut(s)	268
EaeI	YGGCCR	2 cut(s)	1486, 1497
Eam1104I	CTCTTC	5 cut(s)	19, 53, 866, 1752, 2073
Eam1105I	GACNNNNNGTC	1 cut(s)	268
EarI	CTCTTC	5 cut(s)	19, 53, 866, 1752, 2073
Ecl136II	GAGCTC	1 cut(s)	2066
Eco130I	CCWWGG	2 cut(s)	387, 2737
Eco147I	AGGCCT	1 cut(s)	443
Eco24I	GRGCYC	2 cut(s)	1633, 2068
Eco31I	GGTCTC	1 cut(s)	2153
Eco32I	GATATC	1 cut(s)	1326
Eco47I	GGWCC	1 cut(s)	1694
Eco47III	AGCGCT	1 cut(s)	89
Eco53kI	GAGCTC	1 cut(s)	2066
Eco57I	CTGAAG	6 cut(s)	792, 1022, 2176, 2280, 2692, 2706
EcoICRI	GAGCTC	1 cut(s)	2066
EcoRI	GAATTC	2 cut(s)	1998, 2435
EcoRII	CCWGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
EcoRV	GATATC	1 cut(s)	1326
EcoT14I	CCWWGG	2 cut(s)	387, 2737
EcoT38I	GRGCYC	2 cut(s)	1633, 2068
ErhI	CCWWGG	2 cut(s)	387, 2737
FaeI	CATG	8 cut(s)	599, 635, 666, 907, 1904, 1910, 2443, 2539
FalI	AAGNNNNNCTT	6 cut(s)	234, 266, 555, 587, 765, 797
FaqI	GGGAC	1 cut(s)	1349
FatI	CATG	8 cut(s)	595, 631, 662, 903, 1900, 1906, 2439, 2535
FauI	CCCGC	3 cut(s)	1605, 1816, 2494
FbaI	TGATCA	1 cut(s)	2044
Fnu4HI	GCNGC	6 cut(s)	91, 897, 1131, 1898, 2564, 2723
FokI	GGATG	7 cut(s)	712, 1047, 1061, 1178, 2366, 2510, 2571
FriOI	GRGCYC	2 cut(s)	1633, 2068
Fsp4HI	GCNGC	6 cut(s)	91, 897, 1131, 1898, 2564, 2723
FspBI	CTAG	9 cut(s)	840, 1335, 1407, 1650, 1721, 1839, 1959, 2346, 2637
FspI	TGCGCA	1 cut(s)	2177
GlaI	GCGC	2 cut(s)	89, 2177
GluI	GCNGC	6 cut(s)	91, 897, 1131, 1898, 2564, 2723
GsaI	CCCAGC	1 cut(s)	39
GsuI	CTGGAG	4 cut(s)	10, 204, 1491, 2181
HaeII	RGCGCY	1 cut(s)	91
HaeIII	GGCC	5 cut(s)	443, 1488, 1499, 2038, 2671
HapII	CCGG	2 cut(s)	2288, 2402
HgaI	GACGC	3 cut(s)	80, 1424, 2244
HhaI	GCGC	2 cut(s)	90, 2178
Hin1I	GRCGYC	3 cut(s)	5, 72, 1435
Hin1II	CATG	8 cut(s)	599, 635, 666, 907, 1904, 1910, 2443, 2539
Hin6I	GCGC	2 cut(s)	88, 2176
HinP1I	GCGC	2 cut(s)	88, 2176
HincII	GTYRAC	3 cut(s)	1831, 1942, 2533
HindII	GTYRAC	3 cut(s)	1831, 1942, 2533
HpaII	CCGG	2 cut(s)	2288, 2402
HphI	GGTGA	2 cut(s)	119, 272
Hpy166II	GTNNAC	6 cut(s)	1831, 1942, 2234, 2533, 2628, 2744
Hpy8I	GTNNAC	6 cut(s)	1831, 1942, 2234, 2533, 2628, 2744
HpyAV	CCTTC	5 cut(s)	141, 1235, 1366, 2177, 2407
HpyCH4III	ACNGT	7 cut(s)	1466, 1483, 1600, 1933, 2110, 2601, 2748
HpyCH4IV	ACGT	3 cut(s)	1116, 2004, 2520
HpyF10VI	GCNNNNNNNGC	7 cut(s)	71, 85, 596, 1637, 2184, 2270, 2498
HpySE526I	ACGT	3 cut(s)	1116, 2004, 2520
Hsp92I	GRCGYC	3 cut(s)	5, 72, 1435
Hsp92II	CATG	8 cut(s)	599, 635, 666, 907, 1904, 1910, 2443, 2539
HspAI	GCGC	2 cut(s)	88, 2176
Ksp22I	TGATCA	1 cut(s)	2044
LguI	GCTCTTC	2 cut(s)	53, 2073
LmnI	GCTCC	1 cut(s)	2114
Lsp1109I	GCAGC	5 cut(s)	77, 908, 1117, 1909, 2550
MabI	ACCWGGT	1 cut(s)	1696
MaeI	CTAG	9 cut(s)	840, 1335, 1407, 1650, 1721, 1839, 1959, 2346, 2637
MaeII	ACGT	3 cut(s)	1116, 2004, 2520
MaeIII	GTNAC	9 cut(s)	260, 391, 554, 884, 931, 1395, 2008, 2689, 2711
MbiI	CCGCTC	1 cut(s)	132
MfeI	CAATTG	3 cut(s)	536, 694, 816
MflI	RGATCY	2 cut(s)	970, 2290
MhlI	GDGCHC	5 cut(s)	913, 1347, 1633, 2068, 2746
MlsI	TGGCCA	2 cut(s)	1488, 1499
MluNI	TGGCCA	2 cut(s)	1488, 1499
MlyI	GAGTC	5 cut(s)	614, 966, 1196, 1949, 2517
MmeI	TCCRAC	2 cut(s)	2092, 2503
Mox20I	TGGCCA	2 cut(s)	1488, 1499
MroXI	GAANNNNTTC	2 cut(s)	243, 1118
MscI	TGGCCA	2 cut(s)	1488, 1499
MseI	TTAA	7 cut(s)	318, 458, 1554, 1730, 1764, 2133, 2361
MslI	CAYNNNNRTG	4 cut(s)	1079, 1556, 1905, 1977
Msp20I	TGGCCA	2 cut(s)	1488, 1499
MspA1I	CMGCKG	2 cut(s)	267, 1952
MspCI	CTTAAG	1 cut(s)	2360
MspI	CCGG	2 cut(s)	2288, 2402
MspR9I	CCNGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
MunI	CAATTG	3 cut(s)	536, 694, 816
Mva1269I	GAATGC	1 cut(s)	1387
MvaI	CCWGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
MwoI	GCNNNNNNNGC	7 cut(s)	71, 85, 596, 1637, 2184, 2270, 2498
NheI	GCTAGC	1 cut(s)	839
NlaIII	CATG	8 cut(s)	599, 635, 666, 907, 1904, 1910, 2443, 2539
NlaIV	GGNNCC	3 cut(s)	1711, 2292, 2406
NmeAIII	GCCGAG	1 cut(s)	2455
NmuCI	GTSAC	6 cut(s)	260, 884, 931, 2008, 2689, 2711
NsbI	TGCGCA	1 cut(s)	2177
NspI	RCATGY	1 cut(s)	1904
PasI	CCCWGGG	1 cut(s)	1177
PceI	AGGCCT	1 cut(s)	443
PciSI	GCTCTTC	2 cut(s)	53, 2073
PctI	GAATGC	1 cut(s)	1387
PdmI	GAANNNNTTC	2 cut(s)	243, 1118
PfeI	GAWTC	7 cut(s)	350, 727, 997, 1653, 1788, 1912, 2351
PflFI	GACNNNGTC	4 cut(s)	604, 1009, 1698, 2537
PflMI	CCANNNNNTGG	2 cut(s)	1183, 1495
PfoI	TCCNGGA	2 cut(s)	1507, 2151
PkrI	GCNGC	6 cut(s)	92, 898, 1132, 1899, 2565, 2724
PleI	GAGTC	5 cut(s)	613, 965, 1195, 1949, 2516
PpsI	GAGTC	5 cut(s)	613, 965, 1195, 1949, 2516
Ppu21I	YACGTR	1 cut(s)	2005
PshBI	ATTAAT	2 cut(s)	1554, 2133
PsiI	TTATAA	1 cut(s)	1106
Psp124BI	GAGCTC	1 cut(s)	2068
Psp1406I	AACGTT	1 cut(s)	1116
Psp6I	CCWGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
PspFI	CCCAGC	1 cut(s)	35
PspGI	CCWGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
PspN4I	GGNNCC	3 cut(s)	1711, 2292, 2406
PspPI	GGNCC	3 cut(s)	1694, 2036, 2669
PstI	CTGCAG	3 cut(s)	898, 1404, 2568
PstNI	CAGNNNCTG	1 cut(s)	658
PsuI	RGATCY	2 cut(s)	970, 2290
PsyI	GACNNNGTC	4 cut(s)	604, 1009, 1698, 2537
PvuII	CAGCTG	2 cut(s)	267, 1952
RsaI	GTAC	8 cut(s)	292, 487, 1270, 1504, 1589, 1905, 2200, 2573
RsaNI	GTAC	8 cut(s)	291, 486, 1269, 1503, 1588, 1904, 2199, 2572
RseI	CAYNNNNRTG	4 cut(s)	1079, 1556, 1905, 1977
SacI	GAGCTC	1 cut(s)	2068
SapI	GCTCTTC	2 cut(s)	53, 2073
SaqAI	TTAA	7 cut(s)	318, 458, 1554, 1730, 1764, 2133, 2361
SatI	GCNGC	6 cut(s)	91, 897, 1131, 1898, 2564, 2723
Sau96I	GGNCC	3 cut(s)	1694, 2036, 2669
ScaI	AGTACT	1 cut(s)	1504
SchI	GAGTC	5 cut(s)	614, 966, 1196, 1949, 2517
ScrFI	CCNGG	9 cut(s)	82, 1027, 1137, 1178, 1266, 1509, 1698, 2153, 2673
SduI	GDGCHC	5 cut(s)	913, 1347, 1633, 2068, 2746
SexAI	ACCWGGT	1 cut(s)	1696
SfcI	CTRYAG	6 cut(s)	894, 961, 1400, 1465, 1599, 2564
SinI	GGWCC	1 cut(s)	1694
SmiMI	CAYNNNNRTG	4 cut(s)	1079, 1556, 1905, 1977
SmlI	CTYRAG	6 cut(s)	66, 782, 1572, 1751, 2360, 2512
SmoI	CTYRAG	6 cut(s)	66, 782, 1572, 1751, 2360, 2512
SseBI	AGGCCT	1 cut(s)	443
SsiI	CCGC	8 cut(s)	130, 288, 1612, 1823, 1990, 2501, 2700, 2722
SspI	AATATT	4 cut(s)	856, 1594, 2051, 2172
SspMI	CTAG	9 cut(s)	840, 1335, 1407, 1650, 1721, 1839, 1959, 2346, 2637
SstI	GAGCTC	1 cut(s)	2068
StuI	AGGCCT	1 cut(s)	443
StyD4I	CCNGG	9 cut(s)	80, 1025, 1135, 1176, 1264, 1507, 1696, 2151, 2671
StyI	CCWWGG	2 cut(s)	387, 2737
TaaI	ACNGT	7 cut(s)	1466, 1483, 1600, 1933, 2110, 2601, 2748
TaiI	ACGT	3 cut(s)	1119, 2007, 2523
TaqI	TCGA	2 cut(s)	918, 2460
TatI	WGTACW	5 cut(s)	485, 1502, 1587, 1903, 2571
TauI	GCSGC	1 cut(s)	2725
TfiI	GAWTC	7 cut(s)	350, 727, 997, 1653, 1788, 1912, 2351
Tru1I	TTAA	7 cut(s)	318, 458, 1554, 1730, 1764, 2133, 2361
Tru9I	TTAA	7 cut(s)	318, 458, 1554, 1730, 1764, 2133, 2361
TseFI	GTSAC	6 cut(s)	260, 884, 931, 2008, 2689, 2711
TseI	GCWGC	5 cut(s)	90, 896, 1130, 1897, 2563
Tsp45I	GTSAC	6 cut(s)	260, 884, 931, 2008, 2689, 2711
TspDTI	ATGAA	6 cut(s)	266, 1182, 1995, 2115, 2428, 2721
Tth111I	GACNNNGTC	4 cut(s)	604, 1009, 1698, 2537
Van91I	CCANNNNNTGG	2 cut(s)	1183, 1495
Vha464I	CTTAAG	1 cut(s)	2360
VneI	GTGCAC	1 cut(s)	2742
VpaK11BI	GGWCC	1 cut(s)	1694
VspI	ATTAAT	2 cut(s)	1554, 2133
XapI	RAATTY	6 cut(s)	281, 362, 617, 1146, 1998, 2435
XbaI	TCTAGA	3 cut(s)	1406, 1649, 1838
XceI	RCATGY	1 cut(s)	1904
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1678
XmnI	GAANNNNTTC	2 cut(s)	243, 1118
XspI	CTAG	9 cut(s)	840, 1335, 1407, 1650, 1721, 1839, 1959, 2346, 2637
ZrmI	AGTACT	1 cut(s)	1504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.