Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Reverse (-)

66542540 .. 66551526

Gene Structure 8987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015472

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGCGTCGCTCTCGTCGTCGGCGATCTCTAACCACAGCCTCTCCCCCAATGCCTTTGTGCCTAAGAAGATCTGCTTCTTCTCTAATCAATGCCGCGCATTACTTCTCCCTCAGCGCTCTCCTATTTGCAGGATGGGCTTTGTCTCTGGGCTGGAAAGGAAAAAGACTTCTAAAACCAACATCTTTGCATCAGTCACAGACACTTCAGTAGGAACATCCCCTGAGAAAGTCCAGCTTCCAAAAGGTGACAGTTGGTCTGTTCATAAATTTGGGGGCACTTGCATGGGAAGTTCGGAAAGAATTAATAATGTTGCAAAGATAATTCTTAGTGATGAGTCGGAGAGGAAGTTTGTAGTTGTCTCTGCAATGTCTAAGGTAACAGATATGATGTACGATCTCATCTTAAAGGCTCAATCACGAGACGAAACCTATTTGGCTGCATTAGATGCTGTTTTAGTAAAACACAGATCAACAGCTGTCGATTTACTTGATGGGAATGAACTTGGTAGCTTCTTATCAGCATTAAATGAGGACATCAGTAACCTGAAAGCTATGCTTCGTGCAATATATATTGCTGGTCATGCAACAGAATCTTTCACAGATTTTGTGGTAGGACATGGAGAGTTATGGTCTGCTCAGTTGCTGGCATCTGTTGTTAGAAAGAATGGAGTGGATTGCAAGTGGATGGATACAAGGGAAGTCCTTATTGTTAACCCTACTAGCTCTGATCAAGTCGATCCTGATTTCGAGAAATCTGAAAAGAGACTTGAGAAATGGTACTCAAGAAATCCATCAAAAACAATCATAGCTACTGGTTTTATTGCTAGTACACCCCAAGACATTCCTACTACTTTGAAGAGAGATGGAAGTGACTTCTCTGCAGCTATCATGGGTGCTCTATTCAAGGCTGGTCAAGTTACAATTTGGACGGATGTTGATGGTGTCTATAGTGCGGACCCCAGGAAAGTTAGTGAAGCAGTGATTCTGAAGACATTATCTTATCAAGAGGCCTGGGAAATGTCTTATTTTGGGGCAAATGTTCTGCATCCACGTACCATCATTCCAGTAATGCGATATGATATCCCAATTATCATAAGGAACGTTTTCAACCTCTCTGCACCTGGAACAAAAATTTGCCGCGCTTCTACTACTGAGGATGAAGTAGACCAGAGTTTGGAATCCTTCGTCAGAGGGTTTGCGACAATAGACAACTTGGCCCTTGTAAATGTTGAAGGAACTGGAATGGCTGGTGTTCCTGGTACAGCTAGTGCTATATTTTCTGCCGTCAAAGATGTGGGGGCTAATGTTATTATGATATCTCAGGCTAGCAGCGAACATTCTGTATGCTTTGCTGTCCCGGAGAAGGAAGTAAAAGCTGTTTCTAAGGCACTGCAATCTAGGTTCCGTGAAGCTTTGAATGCTGGGCGTCTTTCTCAGGTTCAAGTCATTCCAAACTGTAGTATATTGGCAGCGGTTGGCCAGAAAATGGCAAGTACTCCTGGAGTTAGTGCAACTCTTTTCAATGCCCTCGCAAAGGCAAATATTAACGTGCGTGCTATAGCTCAAGGTTGTTCTGAGTACAACATTACAGTAGTATTAAAGCGGGAGGATTGTATTAGGGCCCTGAGAGCGGTTCACTCTAGATTTTATCTTTCAAGAACCACTATAGCAATGGGCATTGTTGGACCAGGACTTATTGGTGCTACATTACTTGATCAACTGAGAGATCAGGCTGCAACTCTTAAGGAAGAGTTTAACATTGACTTGCGTGTAATGGGAATTACTGGCTCAAGGACAATGCTTTTGAGTGAGACGGGTGTTGACTTATCTAGATGGAGAGAACTTCAACAAGAAAAAGGAGAACGGGCAGATATGGAAAAATTTGTGCAGCATGTACATGGGAATCATTTTATTCCAAATACTGTTCTAGTTGACTGCACAGCTGACTCTGTCATTGCAAGCCATTACTATGGCTGGTTGCGGAAAGGAATACATGTAGTCACCCCCAACAAAAAGGCGAATTCAGGGCCTCTTGATCAGTATTTGAAATTAAGAGCTCTTCAACGGCAATCATATACACATTACTTCTATGAAGCCACTGTTGGAGCTGGCCTCCCAATTATCAATACATTACAAGGGCTCCTAGAAACAGGGGACAAAATATTGCGTATTGAGGGCATCTTCAGTGGGACTTTGAGTTATATCTTCAACAACTTAGTAGGAAGACGAACCTTTAGTGAGGTGGTGGCTGAGGCAAAGCAGGCAGGGTTTACTGAGCCAGACCCAAGGGACGATCTATCTGGAACAGATGTTTGTAGGAAGGTAATAATTCTTGCAAGGGAATCTGGTCTTAAGCTGGAACTCTCTGACATCCCTGTTGAAAGCCTCGTGCCAGAACCATTGAAGGATAGTGCATCAGCTGAGGAATTCATGCAGAAACTTCCACAATTCGATCATGACTTGGCTAAGAAAAGACAGATCGCCGAGGATGCAGGGGAAGTCTTGAGATACGTTGGGGTGGTTGATGTAGTCAACCAGAAGGGGGTAGTGAAGCTGCAAGCATACAAAAACGATCACCCATTTGCTCAATTATCAGGGGCAGATAATATCATTGCTTTCACAACAACAAGGTACAAAGATCAGCCGCTAATAGTCCGTGGACCAGGAGCTGGTGCTGAAGTTACAGCTGGTGGAGTCTTCAGTGACATACTGAGGCTTGCCTCATATCTTGGTGCCCCATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

915

Amino Acids

99.76

Weight (kDa)

7.23

Isoelectric Point (pI)

34.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	86 - 367	2.7e-43	Amino acid kinase family
ACT_7	PF13840	403 - 465	5.6e-06	ACT domain
ACT_9	PF22468	408 - 468	4.7e-18	ACT domain
ACT_7	PF13840	483 - 547	2.4e-09	ACT domain
ACT_9	PF22468	490 - 549	1.4e-21	ACT domain
ACT	PF01842	496 - 536	4e-06	ACT domain
NAD_binding_3	PF03447	562 - 698	2.3e-21	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	706 - 904	3.7e-52	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2732
AccB7I	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
AccBSI	CCGCTC	1 cut(s)	1631
AccI	GTMKAC	1 cut(s)	1164
AccII	CGCG	2 cut(s)	96, 1140
AciI	CCGC	8 cut(s)	94, 953, 1138, 1472, 1603, 1631, 1981, 2645
AclI	AACGTT	1 cut(s)	1101
AclWI	GGATC	1 cut(s)	731
AcoI	YGGCCR	1 cut(s)	1477
AcsI	RAATTY	5 cut(s)	266, 1131, 1880, 2020, 2426
AcuI	CTGAAG	5 cut(s)	189, 1007, 2167, 2683, 2697
AcyI	GRCGYC	2 cut(s)	5, 1426
AfaI	GTAC	9 cut(s)	392, 779, 829, 1054, 1261, 1495, 1580, 1896, 2633
AfeI	AGCGCT	1 cut(s)	116
AfiI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
AflII	CTTAAG	2 cut(s)	1742, 2351
AflIII	ACRYGT	1 cut(s)	1993
AhdI	GACNNNNNGTC	1 cut(s)	253
AjnI	CCWGG	7 cut(s)	959, 1010, 1120, 1255, 1498, 1687, 2662
AjuI	GAANNNNNNNTTGG	2 cut(s)	2085, 2117
Alw21I	GWGCWC	2 cut(s)	898, 2059
Alw26I	GTCTC	5 cut(s)	148, 364, 414, 757, 1805
AlwI	GGATC	1 cut(s)	731
AlwNI	CAGNNNCTG	2 cut(s)	643, 2669
Aor51HI	AGCGCT	1 cut(s)	116
AoxI	GGCC	6 cut(s)	1008, 1215, 1477, 1620, 2027, 2110
ApaI	GGGCCC	1 cut(s)	1624
ApeKI	GCWGC	7 cut(s)	437, 881, 1329, 1469, 1733, 1888, 2554
ApoI	RAATTY	5 cut(s)	266, 1131, 1880, 2020, 2426
ArsI	GACNNNNNNTTYG	2 cut(s)	1786, 1818
AseI	ATTAAT	1 cut(s)	303
Asp700I	GAANNNNTTC	1 cut(s)	1103
AspLEI	GCGC	3 cut(s)	98, 117, 1142
AspS9I	GGNCC	7 cut(s)	955, 1216, 1620, 1621, 1685, 2027, 2660
AsuC2I	CCSGG	1 cut(s)	1358
AsuHPI	GGTGA	3 cut(s)	257, 1993, 2567
AsuNHI	GCTAGC	1 cut(s)	1325
AvaII	GGWCC	3 cut(s)	955, 1685, 2660
BaeGI	GKGCMC	3 cut(s)	278, 1624, 2737
BalI	TGGCCA	1 cut(s)	1479
BanI	GGYRCC	1 cut(s)	2732
BanII	GRGCYC	3 cut(s)	1624, 2059, 2142
BauI	CACGAG	2 cut(s)	417, 2387
BbsI	GAAGAC	3 cut(s)	995, 2230, 2689
Bbv12I	GWGCWC	2 cut(s)	898, 2059
BbvCI	CCTCAGC	3 cut(s)	111, 2250, 2421
BbvI	GCAGC	7 cut(s)	424, 893, 1341, 1481, 1720, 1900, 2541
BccI	CCATC	8 cut(s)	127, 485, 679, 799, 857, 932, 1064, 1827
BceAI	ACGGC	2 cut(s)	1268, 2081
BciT130I	CCWGG	7 cut(s)	961, 1012, 1122, 1257, 1500, 1689, 2664
BciVI	GTATCC	1 cut(s)	682
BclI	TGATCA	3 cut(s)	727, 1714, 2035
BcnI	CCSGG	1 cut(s)	1358
BcoDI	GTCTC	5 cut(s)	148, 364, 414, 757, 1805
BfaI	CTAG	9 cut(s)	720, 825, 1266, 1326, 1398, 1641, 1830, 1928, 2144
BfmI	CTRYAG	5 cut(s)	879, 946, 1456, 1557, 1665
BfoI	RGCGCY	1 cut(s)	118
BfrI	CTTAAG	2 cut(s)	1742, 2351
BfuI	GTATCC	1 cut(s)	682
BglII	AGATCT	1 cut(s)	69
BmcAI	AGTACT	1 cut(s)	1495
Bme1390I	CCNGG	8 cut(s)	961, 1012, 1122, 1257, 1358, 1500, 1689, 2664
Bme18I	GGWCC	3 cut(s)	955, 1685, 2660
BmeRI	GACNNNNNGTC	1 cut(s)	253
BmgT120I	GGNCC	7 cut(s)	955, 1216, 1620, 1621, 1685, 2027, 2660
BmiI	GGNNCC	5 cut(s)	957, 1403, 1622, 2141, 2734
BmrFI	CCNGG	8 cut(s)	961, 1012, 1122, 1257, 1358, 1500, 1689, 2664
BmsI	GCATC	7 cut(s)	197, 436, 656, 1054, 2187, 2423, 2479
BmtI	GCTAGC	1 cut(s)	1329
BpiI	GAAGAC	3 cut(s)	995, 2230, 2689
BplI	GAGNNNNNCTC	2 cut(s)	2097, 2129
BpmI	CTGGAG	1 cut(s)	1521
Bpu10I	CCTNAGC	3 cut(s)	111, 2250, 2421
BpuEI	CTTGAG	5 cut(s)	766, 788, 1548, 1774, 2524
BpuMI	CCSGG	1 cut(s)	1358
BsaAI	YACGTR	1 cut(s)	1052
BsaHI	GRCGYC	2 cut(s)	5, 1426
BsaJI	CCNNGG	5 cut(s)	959, 1011, 2285, 2484, 2656
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 332, 362, 2124, 2154
Bsc4I	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
Bse1I	ACTGG	4 cut(s)	817, 1064, 1243, 1789
Bse3DI	GCAATG	4 cut(s)	372, 1677, 1953, 2610
BseBI	CCWGG	7 cut(s)	961, 1012, 1122, 1257, 1500, 1689, 2664
BseDI	CCNNGG	5 cut(s)	959, 1011, 2285, 2484, 2656
BseGI	GGATG	8 cut(s)	138, 215, 690, 937, 1045, 1162, 2370, 2494
BseLI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
BseMI	GCAATG	4 cut(s)	372, 1677, 1953, 2610
BseNI	ACTGG	4 cut(s)	817, 1064, 1243, 1789
BseSI	GKGCMC	3 cut(s)	278, 1624, 2737
BseXI	GCAGC	7 cut(s)	424, 893, 1341, 1481, 1720, 1900, 2541
BseYI	CCCAGC	1 cut(s)	1421
BsgI	GTGCAG	3 cut(s)	1101, 1907, 1921
Bsh1236I	CGCG	2 cut(s)	96, 1140
BshFI	GGCC	6 cut(s)	1010, 1217, 1479, 1622, 2029, 2112
BshNI	GGYRCC	1 cut(s)	2732
BsiHKAI	GWGCWC	2 cut(s)	898, 2059
BsiSI	CCGG	1 cut(s)	1358
BslFI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
BslI	CCNNNNNNNGG	7 cut(s)	1174, 1363, 1486, 1534, 1630, 2542, 2669
BsmAI	GTCTC	5 cut(s)	148, 364, 414, 757, 1805
BsmBI	CGTCTC	2 cut(s)	414, 1805
BsmFI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
BsmI	GAATGC	1 cut(s)	1423
BsnI	GGCC	6 cut(s)	1010, 1217, 1479, 1622, 2029, 2112
Bsp120I	GGGCCC	1 cut(s)	1620
Bsp1286I	GDGCHC	6 cut(s)	278, 898, 1624, 2059, 2142, 2737
Bsp1407I	TGTACA	1 cut(s)	1894
BspACI	CCGC	8 cut(s)	94, 953, 1138, 1472, 1603, 1631, 1981, 2645
BspANI	GGCC	6 cut(s)	1010, 1217, 1479, 1622, 2029, 2112
BspFNI	CGCG	2 cut(s)	96, 1140
BspHI	TCATGA	1 cut(s)	2455
BspLI	GGNNCC	5 cut(s)	957, 1403, 1622, 2141, 2734
BspMAI	CTGCAG	1 cut(s)	883
BspOI	GCTAGC	1 cut(s)	1329
BspPI	GGATC	1 cut(s)	731
BspQI	GCTCTTC	1 cut(s)	2064
BspT107I	GGYRCC	1 cut(s)	2732
BspTI	CTTAAG	2 cut(s)	1742, 2351
BsrBI	CCGCTC	1 cut(s)	1631
BsrDI	GCAATG	4 cut(s)	372, 1677, 1953, 2610
BsrGI	TGTACA	1 cut(s)	1894
BsrI	ACTGG	4 cut(s)	817, 1064, 1243, 1789
BssECI	CCNNGG	5 cut(s)	959, 1011, 2285, 2484, 2656
BssNI	GRCGYC	2 cut(s)	5, 1426
BssSI	CACGAG	2 cut(s)	417, 2387
BssT1I	CCWWGG	1 cut(s)	2285
Bst2BI	CACGAG	2 cut(s)	417, 2387
Bst2UI	CCWGG	7 cut(s)	961, 1012, 1122, 1257, 1500, 1689, 2664
Bst4CI	ACNGT	5 cut(s)	251, 1457, 1591, 1924, 2101
Bst6I	CTCTTC	3 cut(s)	851, 1743, 2064
BstACI	GRCGYC	2 cut(s)	5, 1426
BstAFI	CTTAAG	2 cut(s)	1742, 2351
BstAPI	GCANNNNNTGC	1 cut(s)	446
BstAUI	TGTACA	1 cut(s)	1894
BstBAI	YACGTR	1 cut(s)	1052
BstC8I	GCNNGC	8 cut(s)	645, 1327, 1554, 1960, 2110, 2262, 2559, 2718
BstDSI	CCRYGG	1 cut(s)	2656
BstENI	CCTNNNNNAGG	1 cut(s)	1532
BstF5I	GGATG	8 cut(s)	138, 215, 690, 937, 1045, 1162, 2370, 2494
BstFNI	CGCG	2 cut(s)	96, 1140
BstH2I	RGCGCY	1 cut(s)	118
BstHHI	GCGC	3 cut(s)	98, 117, 1142
BstMAI	GTCTC	5 cut(s)	148, 364, 414, 757, 1805
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 446, 581, 1418, 1628, 2261, 2489
BstNI	CCWGG	7 cut(s)	961, 1012, 1122, 1257, 1500, 1689, 2664
BstNSI	RCATGY	2 cut(s)	1895, 1997
BstSCI	CCNGG	8 cut(s)	959, 1010, 1120, 1255, 1356, 1498, 1687, 2662
BstSFI	CTRYAG	5 cut(s)	879, 946, 1456, 1557, 1665
BstSLI	GKGCMC	3 cut(s)	278, 1624, 2737
BstUI	CGCG	2 cut(s)	96, 1140
BstV1I	GCAGC	7 cut(s)	424, 893, 1341, 1481, 1720, 1900, 2541
BstV2I	GAAGAC	3 cut(s)	995, 2230, 2689
BstX2I	RGATCY	1 cut(s)	69
BstXI	CCANNNNNNTGG	1 cut(s)	1970
BstYI	RGATCY	1 cut(s)	69
BsuI	GTATCC	1 cut(s)	682
BsuRI	GGCC	6 cut(s)	1010, 1217, 1479, 1622, 2029, 2112
BtgI	CCRYGG	1 cut(s)	2656
BtsCI	GGATG	8 cut(s)	138, 215, 690, 937, 1045, 1162, 2370, 2494
BtsI	GCAGTG	2 cut(s)	984, 1388
BtsIMutI	CAGTG	5 cut(s)	984, 1388, 2097, 2191, 2707
Cac8I	GCNNGC	8 cut(s)	645, 1327, 1554, 1960, 2110, 2262, 2559, 2718
CaiI	CAGNNNCTG	2 cut(s)	643, 2669
CciI	TCATGA	1 cut(s)	2455
CfoI	GCGC	3 cut(s)	98, 117, 1142
Cfr13I	GGNCC	7 cut(s)	955, 1216, 1620, 1621, 1685, 2027, 2660
CseI	GACGC	1 cut(s)	1415
Csp6I	GTAC	9 cut(s)	391, 778, 828, 1053, 1260, 1494, 1579, 1895, 2632
CviAII	CATG	9 cut(s)	283, 581, 617, 889, 1892, 1898, 1994, 2431, 2456
CviQI	GTAC	9 cut(s)	391, 778, 828, 1053, 1260, 1494, 1579, 1895, 2632
DriI	GACNNNNNGTC	1 cut(s)	253
EaeI	YGGCCR	1 cut(s)	1477
Eam1104I	CTCTTC	3 cut(s)	851, 1743, 2064
Eam1105I	GACNNNNNGTC	1 cut(s)	253
EarI	CTCTTC	3 cut(s)	851, 1743, 2064
Ecl136II	GAGCTC	1 cut(s)	2057
Eco130I	CCWWGG	1 cut(s)	2285
Eco147I	AGGCCT	1 cut(s)	1010
Eco24I	GRGCYC	3 cut(s)	1624, 2059, 2142
Eco32I	GATATC	2 cut(s)	1081, 1317
Eco47I	GGWCC	3 cut(s)	955, 1685, 2660
Eco47III	AGCGCT	1 cut(s)	116
Eco53kI	GAGCTC	1 cut(s)	2057
Eco57I	CTGAAG	5 cut(s)	189, 1007, 2167, 2683, 2697
EcoICRI	GAGCTC	1 cut(s)	2057
EcoNI	CCTNNNNNAGG	1 cut(s)	1532
EcoO109I	RGGNCCY	3 cut(s)	1620, 1621, 2027
EcoRI	GAATTC	2 cut(s)	2020, 2426
EcoRII	CCWGG	7 cut(s)	959, 1010, 1120, 1255, 1498, 1687, 2662
EcoRV	GATATC	2 cut(s)	1081, 1317
EcoT14I	CCWWGG	1 cut(s)	2285
EcoT38I	GRGCYC	3 cut(s)	1624, 2059, 2142
ErhI	CCWWGG	1 cut(s)	2285
Esp3I	CGTCTC	2 cut(s)	414, 1805
FaeI	CATG	9 cut(s)	286, 584, 620, 892, 1895, 1901, 1997, 2434, 2459
FalI	AAGNNNNNCTT	6 cut(s)	59, 91, 219, 251, 540, 572
FaqI	GGGAC	4 cut(s)	1340, 2168, 2203, 2303
FatI	CATG	9 cut(s)	282, 580, 616, 888, 1891, 1897, 1993, 2430, 2455
FauI	CCCGC	1 cut(s)	1596
FbaI	TGATCA	3 cut(s)	727, 1714, 2035
FblI	GTMKAC	1 cut(s)	1164
FokI	GGATG	8 cut(s)	145, 202, 697, 944, 1032, 1169, 2357, 2501
FriOI	GRGCYC	3 cut(s)	1624, 2059, 2142
FspBI	CTAG	9 cut(s)	720, 825, 1266, 1326, 1398, 1641, 1830, 1928, 2144
GlaI	GCGC	3 cut(s)	97, 116, 1141
GsaI	CCCAGC	1 cut(s)	1425
GsuI	CTGGAG	1 cut(s)	1521
HaeII	RGCGCY	1 cut(s)	118
HaeIII	GGCC	6 cut(s)	1010, 1217, 1479, 1622, 2029, 2112
HapII	CCGG	1 cut(s)	1358
HgaI	GACGC	1 cut(s)	1415
HhaI	GCGC	3 cut(s)	98, 117, 1142
Hin1I	GRCGYC	2 cut(s)	5, 1426
Hin1II	CATG	9 cut(s)	286, 584, 620, 892, 1895, 1901, 1997, 2434, 2459
Hin6I	GCGC	3 cut(s)	96, 115, 1140
HinP1I	GCGC	3 cut(s)	96, 115, 1140
HincII	GTYRAC	4 cut(s)	712, 1822, 1933, 2533
HindII	GTYRAC	4 cut(s)	712, 1822, 1933, 2533
HindIII	AAGCTT	1 cut(s)	1410
HinfI	GANTC	8 cut(s)	335, 590, 982, 1178, 1903, 1946, 2342, 2694
HpaI	GTTAAC	1 cut(s)	712
HpaII	CCGG	1 cut(s)	1358
HphI	GGTGA	3 cut(s)	257, 1993, 2567
Hpy166II	GTNNAC	9 cut(s)	712, 830, 1165, 1636, 1822, 1933, 2271, 2533, 2660
Hpy188I	TCNGA	8 cut(s)	295, 340, 727, 757, 987, 1190, 1576, 2368
Hpy8I	GTNNAC	9 cut(s)	712, 830, 1165, 1636, 1822, 1933, 2271, 2533, 2660
Hpy99I	CGWCG	3 cut(s)	10, 19, 22
HpyAV	CCTTC	6 cut(s)	1192, 1226, 1357, 2314, 2398, 2533
HpyCH4III	ACNGT	5 cut(s)	251, 1457, 1591, 1924, 2101
HpyCH4IV	ACGT	4 cut(s)	1051, 1101, 1548, 2511
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 446, 581, 1418, 1628, 2261, 2489
HpySE526I	ACGT	4 cut(s)	1051, 1101, 1548, 2511
Hsp92I	GRCGYC	2 cut(s)	5, 1426
Hsp92II	CATG	9 cut(s)	286, 584, 620, 892, 1895, 1901, 1997, 2434, 2459
HspAI	GCGC	3 cut(s)	96, 115, 1140
Ksp22I	TGATCA	3 cut(s)	727, 1714, 2035
KspAI	GTTAAC	1 cut(s)	712
LguI	GCTCTTC	1 cut(s)	2064
LmnI	GCTCC	3 cut(s)	2105, 2145, 2666
Lsp1109I	GCAGC	7 cut(s)	424, 893, 1341, 1481, 1720, 1900, 2541
LweI	GCATC	7 cut(s)	197, 436, 656, 1054, 2187, 2423, 2479
MaeI	CTAG	9 cut(s)	720, 825, 1266, 1326, 1398, 1641, 1830, 1928, 2144
MaeII	ACGT	4 cut(s)	1051, 1101, 1548, 2511
MaeIII	GTNAC	9 cut(s)	193, 245, 376, 539, 869, 916, 1999, 2680, 2702
MbiI	CCGCTC	1 cut(s)	1631
MflI	RGATCY	1 cut(s)	69
MhlI	GDGCHC	6 cut(s)	278, 898, 1624, 2059, 2142, 2737
MlsI	TGGCCA	1 cut(s)	1479
MluNI	TGGCCA	1 cut(s)	1479
MlyI	GAGTC	3 cut(s)	344, 1940, 2703
MmeI	TCCRAC	3 cut(s)	318, 1663, 2083
Mox20I	TGGCCA	1 cut(s)	1479
MroXI	GAANNNNTTC	1 cut(s)	1103
MscI	TGGCCA	1 cut(s)	1479
MslI	CAYNNNNRTG	3 cut(s)	281, 1896, 1968
Msp20I	TGGCCA	1 cut(s)	1479
MspA1I	CMGCKG	5 cut(s)	476, 1472, 1943, 2420, 2687
MspCI	CTTAAG	2 cut(s)	1742, 2351
MspI	CCGG	1 cut(s)	1358
MspR9I	CCNGG	8 cut(s)	961, 1012, 1122, 1257, 1358, 1500, 1689, 2664
Mva1269I	GAATGC	1 cut(s)	1423
MvaI	CCWGG	7 cut(s)	961, 1012, 1122, 1257, 1500, 1689, 2664
MvnI	CGCG	2 cut(s)	96, 1140
MwoI	GCNNNNNNNGC	7 cut(s)	135, 446, 581, 1418, 1628, 2261, 2489
NciI	CCSGG	1 cut(s)	1358
NheI	GCTAGC	1 cut(s)	1325
NlaIII	CATG	9 cut(s)	286, 584, 620, 892, 1895, 1901, 1997, 2434, 2459
NlaIV	GGNNCC	5 cut(s)	957, 1403, 1622, 2141, 2734
NmeAIII	GCCGAG	1 cut(s)	2509
NmuCI	GTSAC	5 cut(s)	193, 245, 869, 1999, 2702
NspI	RCATGY	2 cut(s)	1895, 1997
PagI	TCATGA	1 cut(s)	2455
PceI	AGGCCT	1 cut(s)	1010
PciI	ACATGT	1 cut(s)	1993
PciSI	GCTCTTC	1 cut(s)	2064
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 20
PctI	GAATGC	1 cut(s)	1423
PdmI	GAANNNNTTC	1 cut(s)	1103
PfeI	GAWTC	5 cut(s)	590, 982, 1178, 1903, 2342
PflFI	GACNNNGTC	1 cut(s)	1949
PflMI	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
PfoI	TCCNGGA	2 cut(s)	1356, 1498
PleI	GAGTC	3 cut(s)	343, 1940, 2702
PpsI	GAGTC	3 cut(s)	343, 1940, 2702
Ppu21I	YACGTR	1 cut(s)	1052
PscI	ACATGT	1 cut(s)	1993
PshBI	ATTAAT	1 cut(s)	303
Psp124BI	GAGCTC	1 cut(s)	2059
Psp1406I	AACGTT	1 cut(s)	1101
Psp6I	CCWGG	7 cut(s)	959, 1010, 1120, 1255, 1498, 1687, 2662
PspFI	CCCAGC	1 cut(s)	1421
PspGI	CCWGG	7 cut(s)	959, 1010, 1120, 1255, 1498, 1687, 2662
PspN4I	GGNNCC	5 cut(s)	957, 1403, 1622, 2141, 2734
PspOMI	GGGCCC	1 cut(s)	1620
PspPI	GGNCC	7 cut(s)	955, 1216, 1620, 1621, 1685, 2027, 2660
PstI	CTGCAG	1 cut(s)	883
PstNI	CAGNNNCTG	2 cut(s)	643, 2669
PsuI	RGATCY	1 cut(s)	69
PsyI	GACNNNGTC	1 cut(s)	1949
PvuII	CAGCTG	4 cut(s)	476, 1943, 2420, 2687
RsaI	GTAC	9 cut(s)	392, 779, 829, 1054, 1261, 1495, 1580, 1896, 2633
RsaNI	GTAC	9 cut(s)	391, 778, 828, 1053, 1260, 1494, 1579, 1895, 2632
RseI	CAYNNNNRTG	3 cut(s)	281, 1896, 1968
SacI	GAGCTC	1 cut(s)	2059
SapI	GCTCTTC	1 cut(s)	2064
Sau96I	GGNCC	7 cut(s)	955, 1216, 1620, 1621, 1685, 2027, 2660
ScaI	AGTACT	1 cut(s)	1495
SchI	GAGTC	3 cut(s)	344, 1940, 2703
ScrFI	CCNGG	8 cut(s)	961, 1012, 1122, 1257, 1358, 1500, 1689, 2664
SduI	GDGCHC	6 cut(s)	278, 898, 1624, 2059, 2142, 2737
SfaNI	GCATC	7 cut(s)	197, 436, 656, 1054, 2187, 2423, 2479
SfcI	CTRYAG	5 cut(s)	879, 946, 1456, 1557, 1665
SinI	GGWCC	3 cut(s)	955, 1685, 2660
SmiMI	CAYNNNNRTG	3 cut(s)	281, 1896, 1968
SmlI	CTYRAG	7 cut(s)	767, 781, 1563, 1742, 1789, 2351, 2503
SmoI	CTYRAG	7 cut(s)	767, 781, 1563, 1742, 1789, 2351, 2503
SseBI	AGGCCT	1 cut(s)	1010
SsiI	CCGC	8 cut(s)	94, 953, 1138, 1472, 1603, 1631, 1981, 2645
SspI	AATATT	2 cut(s)	1543, 2163
SspMI	CTAG	9 cut(s)	720, 825, 1266, 1326, 1398, 1641, 1830, 1928, 2144
SstI	GAGCTC	1 cut(s)	2059
StuI	AGGCCT	1 cut(s)	1010
StyD4I	CCNGG	8 cut(s)	959, 1010, 1120, 1255, 1356, 1498, 1687, 2662
StyI	CCWWGG	1 cut(s)	2285
TaaI	ACNGT	5 cut(s)	251, 1457, 1591, 1924, 2101
TaiI	ACGT	4 cut(s)	1054, 1104, 1551, 2514
TaqI	TCGA	4 cut(s)	480, 735, 747, 2451
TatI	WGTACW	4 cut(s)	827, 1493, 1578, 1894
TauI	GCSGC	3 cut(s)	96, 1140, 2647
TfiI	GAWTC	5 cut(s)	590, 982, 1178, 1903, 2342
TscAI	CASTG	5 cut(s)	984, 1395, 2104, 2191, 2707
TseFI	GTSAC	5 cut(s)	193, 245, 869, 1999, 2702
TseI	GCWGC	7 cut(s)	437, 881, 1329, 1469, 1733, 1888, 2554
Tsp45I	GTSAC	5 cut(s)	193, 245, 869, 1999, 2702
TspDTI	ATGAA	5 cut(s)	251, 513, 1173, 2106, 2419
TspGWI	ACGGA	3 cut(s)	944, 1394, 2645
TspRI	CASTG	5 cut(s)	984, 1395, 2104, 2191, 2707
Tth111I	GACNNNGTC	1 cut(s)	1949
Van91I	CCANNNNNTGG	3 cut(s)	1174, 1486, 2669
Vha464I	CTTAAG	2 cut(s)	1742, 2351
VpaK11BI	GGWCC	3 cut(s)	955, 1685, 2660
VspI	ATTAAT	1 cut(s)	303
XagI	CCTNNNNNAGG	1 cut(s)	1532
XapI	RAATTY	5 cut(s)	266, 1131, 1880, 2020, 2426
XbaI	TCTAGA	2 cut(s)	1640, 1829
XceI	RCATGY	2 cut(s)	1895, 1997
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1669
XmiI	GTMKAC	1 cut(s)	1164
XmnI	GAANNNNTTC	1 cut(s)	1103
XspI	CTAG	9 cut(s)	720, 825, 1266, 1326, 1398, 1641, 1830, 1928, 2144
ZrmI	AGTACT	1 cut(s)	1495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.