AT1G32410

Vacuolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008726
Physical Location & Seq
Forward (+)
11693799 .. 11695575
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32410.2

Sequence Viewer

Length: 423 bp
ATGGCAGACGTACCTGGATACCTGCGCACTTGTTTGGATATGGGGAAGATAGCTTTCTTAGCAATCCTTGTATCTACAGGGATTGTGTTGCAGATTCTGGCTTGTGCTCTGTTCAATAACTGGTGGCCAATGCTAAGTGTTATAATGTATGTGCTCCTCCCAATGCCTTTGCTCTTCTTTGGTGGATCAGACAGTACATCTCTATTCAACGAGTCAGATAATAGTTGGATCAATGCAGCCAAGTTCTTGACTGGTGCATCAGCAGTTGGAAGCGTCGCGATACCATCGATTCTAAAACACGCCGGACTCATCGGTTGGGGCGCATTAGCCCTTGATCTCTCATCCTACGTTGTCTTCCTCGTGGCTATTTTGGGTTACATTTGCATAGGAGATGCCAGTGACAACTATTACAGCTACATTTAA

Protein Analysis

140

Amino Acids

15.02

Weight (kDa)

4.35

Isoelectric Point (pI)

29.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 17 - 131 3.4e-34 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
Acc16I TGCGCA 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 30
AccII CGCG 1 cut(s) 278
AclWI GGATC 2 cut(s) 193, 236
AcoI YGGCCR 1 cut(s) 125
AfaI GTAC 2 cut(s) 12, 196
AgsI TTSAA 2 cut(s) 115, 208
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 2 cut(s) 53, 414
AluI AGCT 2 cut(s) 53, 414
Alw21I GWGCWC 2 cut(s) 109, 156
AlwI GGATC 2 cut(s) 193, 236
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 236
AspLEI GCGC 2 cut(s) 27, 323
BalI TGGCCA 1 cut(s) 127
BauI CACGAG 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 346
Bbv12I GWGCWC 2 cut(s) 109, 156
BbvI GCAGC 1 cut(s) 248
BccI CCATC 1 cut(s) 292
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 30
BfuI GTATCC 1 cut(s) 11
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 2 cut(s) 266, 382
BpiI GAAGAC 1 cut(s) 346
Bsa29I ATCGAT 1 cut(s) 287
Bse1I ACTGG 3 cut(s) 125, 256, 396
BseBI CCWGG 1 cut(s) 15
BseCI ATCGAT 1 cut(s) 287
BseGI GGATG 1 cut(s) 341
BseNI ACTGG 3 cut(s) 125, 256, 396
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 127
BshVI ATCGAT 1 cut(s) 287
BsiHKAI GWGCWC 2 cut(s) 109, 156
BsiSI CCGG 1 cut(s) 303
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 109, 156
Bsp143I GATC 3 cut(s) 185, 228, 334
Bsp68I TCGCGA 1 cut(s) 278
BspANI GGCC 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 287
BspFNI CGCG 1 cut(s) 278
BspMI ACCTGC 1 cut(s) 30
BspPI GGATC 2 cut(s) 193, 236
BspQI GCTCTTC 1 cut(s) 179
BsrI ACTGG 3 cut(s) 125, 256, 396
BssMI GATC 3 cut(s) 185, 228, 334
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 179
BstDEI CTNAG 2 cut(s) 58, 134
BstF5I GGATG 1 cut(s) 341
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 2 cut(s) 27, 323
BstKTI GATC 3 cut(s) 188, 231, 337
BstMBI GATC 3 cut(s) 185, 228, 334
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 75
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 287
BsuI GTATCC 1 cut(s) 11
BsuRI GGCC 1 cut(s) 127
BsuTUI ATCGAT 1 cut(s) 287
BtsCI GGATG 1 cut(s) 341
BtsIMutI CAGTG 1 cut(s) 403
BtuMI TCGCGA 1 cut(s) 278
BveI ACCTGC 1 cut(s) 30
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 2 cut(s) 27, 323
ClaI ATCGAT 1 cut(s) 287
CseI GACGC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 11, 195
CviJI RGCY 7 cut(s) 53, 101, 127, 239, 329, 365, 414
CviKI_1 RGCY 7 cut(s) 53, 101, 127, 239, 329, 365, 414
CviQI GTAC 2 cut(s) 11, 195
DdeI CTNAG 2 cut(s) 58, 134
DpnI GATC 3 cut(s) 187, 230, 336
DpnII GATC 3 cut(s) 185, 228, 334
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 13
FaiI YATR 4 cut(s) 41, 143, 150, 386
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 237
FspI TGCGCA 1 cut(s) 26
GlaI GCGC 2 cut(s) 26, 322
GluI GCNGC 1 cut(s) 237
HaeIII GGCC 1 cut(s) 127
HapII CCGG 1 cut(s) 303
HgaI GACGC 1 cut(s) 262
HhaI GCGC 2 cut(s) 27, 323
Hin6I GCGC 2 cut(s) 25, 321
HinP1I GCGC 2 cut(s) 25, 321
HinfI GANTC 4 cut(s) 94, 212, 289, 306
HpaII CCGG 1 cut(s) 303
Hpy188I TCNGA 2 cut(s) 190, 217
Hpy188III TCNNGA 2 cut(s) 247, 277
Hpy99I CGWCG 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 2 cut(s) 9, 348
HpyCH4V TGCA 4 cut(s) 91, 236, 257, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 58, 134
HpySE526I ACGT 2 cut(s) 9, 348
HspAI GCGC 2 cut(s) 25, 321
Kzo9I GATC 3 cut(s) 185, 228, 334
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 159
LpnPI CCDG 8 cut(s) 27, 35, 63, 83, 106, 237, 316, 409
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 2 cut(s) 266, 382
MaeII ACGT 2 cut(s) 9, 348
MaeIII GTNAC 2 cut(s) 374, 398
MalI GATC 3 cut(s) 187, 230, 336
MboI GATC 3 cut(s) 185, 228, 334
MboII GAAGA 3 cut(s) 58, 166, 346
MhlI GDGCHC 2 cut(s) 109, 156
MlsI TGGCCA 1 cut(s) 127
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 2 cut(s) 221, 300
MmeI TCCRAC 2 cut(s) 206, 247
MnlI CCTC 2 cut(s) 167, 368
Mox20I TGGCCA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 127
MseI TTAA 1 cut(s) 421
Msp20I TGGCCA 1 cut(s) 127
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 3 cut(s) 185, 228, 334
NmuCI GTSAC 1 cut(s) 398
NruI TCGCGA 1 cut(s) 278
NsbI TGCGCA 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 94, 289
PkrI GCNGC 1 cut(s) 238
PleI GAGTC 2 cut(s) 220, 300
PpsI GAGTC 2 cut(s) 220, 300
PsiI TTATAA 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 97
RruI TCGCGA 1 cut(s) 278
RsaI GTAC 2 cut(s) 12, 196
RsaNI GTAC 2 cut(s) 11, 195
SapI GCTCTTC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 3 cut(s) 185, 228, 334
SchI GAGTC 2 cut(s) 221, 300
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 2 cut(s) 109, 156
SetI ASST 6 cut(s) 12, 16, 24, 55, 351, 416
SfaNI GCATC 2 cut(s) 266, 382
SfcI CTRYAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 2 cut(s) 12, 351
TaqI TCGA 1 cut(s) 287
TatI WGTACW 1 cut(s) 194
TfiI GAWTC 2 cut(s) 94, 289
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 1 cut(s) 403
TseFI GTSAC 1 cut(s) 398
TseI GCWGC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 398
TspRI CASTG 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.