FvH4_5g21150

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008726
Physical Location & Seq
Forward (+)
12706083 .. 12709678
3596 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21150.t1

Sequence Viewer

Length: 414 bp
ATGGCTGACTTACCACGTTATATGCGTCTCTGCTTGCAATCTGGGAAACTTGCTTTCTTGGCAATTTTGGTATCTGGAGGGATTGTACTGCAAATTTTGGCATGTGCTTTGTACAATAATTGGTGGCCGATGCTAACTGTAATAATGTATGTGCTTCTTCCCATGCCGTTGCTGTTCTTTGTGGGATCAGATTTTTCTGTTTTCATGGAATCTGATAATGCCTGGGTTAACGTAACCAAGTTCTTGACTGGAGCTTCAGCCATTGGAAGCATTGCGATACCCGCCATCTTAACACACGCTGGTGTTATTGGCTGGGGAGCCCTGGCCTTGGAGCTCTCATCCTTTTTCGTATTTGTACTCGCCATAATGTGTTACATCCGGACAGACGATGACGACAGCTACAGTTCGATCTGA

Protein Analysis

138

Amino Acids

15.03

Weight (kDa)

4.47

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 17 - 131 8.4e-33 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 1 cut(s) 282
AclWI GGATC 1 cut(s) 193
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 87, 113, 357
AfiI CCNNNNNNNGG 1 cut(s) 328
AjnI CCWGG 2 cut(s) 221, 321
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 3 cut(s) 254, 334, 399
AluI AGCT 3 cut(s) 254, 334, 399
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 2 cut(s) 125, 324
ApoI RAATTY 1 cut(s) 93
BanII GRGCYC 2 cut(s) 322, 336
Bbv12I GWGCWC 1 cut(s) 336
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 223, 323
BcoDI GTCTC 1 cut(s) 32
BfmI CTRYAG 1 cut(s) 400
Bme1390I CCNGG 2 cut(s) 223, 323
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 2 cut(s) 223, 323
BmsI GCATC 1 cut(s) 120
BpmI CTGGAG 2 cut(s) 96, 270
BsaJI CCNNGG 3 cut(s) 222, 321, 327
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 2 cut(s) 223, 323
BseDI CCNNGG 3 cut(s) 222, 321, 327
BseGI GGATG 2 cut(s) 338, 375
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 312
BshFI GGCC 2 cut(s) 127, 326
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 32
BsmBI CGTCTC 1 cut(s) 32
BsnI GGCC 2 cut(s) 127, 326
Bsp1286I GDGCHC 2 cut(s) 322, 336
Bsp13I TCCGGA 1 cut(s) 378
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 185, 408
BspACI CCGC 1 cut(s) 282
BspANI GGCC 2 cut(s) 127, 326
BspEI TCCGGA 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 1 cut(s) 193
BsrDI GCAATG 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 222, 321, 327
BssMI GATC 2 cut(s) 185, 408
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 223, 323
Bst4CI ACNGT 2 cut(s) 139, 404
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 35
BstF5I GGATG 2 cut(s) 338, 375
BstKTI GATC 2 cut(s) 188, 411
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 185, 408
BstMWI GCNNNNNNNGC 2 cut(s) 59, 281
BstNI CCWGG 2 cut(s) 223, 323
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 221, 321
BstSFI CTRYAG 1 cut(s) 400
BsuRI GGCC 2 cut(s) 127, 326
BtsCI GGATG 2 cut(s) 338, 375
Cac8I GCNNGC 1 cut(s) 35
CseI GACGC 1 cut(s) 14
Csp6I GTAC 3 cut(s) 86, 112, 356
CviAII CATG 3 cut(s) 102, 163, 205
CviJI RGCY 9 cut(s) 5, 127, 254, 260, 312, 320, 326, 334, 399
CviKI_1 RGCY 9 cut(s) 5, 127, 254, 260, 312, 320, 326, 334, 399
CviQI GTAC 3 cut(s) 86, 112, 356
DpnI GATC 2 cut(s) 187, 410
DpnII GATC 2 cut(s) 185, 408
EaeI YGGCCR 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 322, 336
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 221, 321
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 322, 336
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 3 cut(s) 105, 166, 208
FaiI YATR 7 cut(s) 21, 23, 103, 150, 164, 206, 365
FatI CATG 3 cut(s) 101, 162, 204
FauI CCCGC 1 cut(s) 289
FokI GGATG 2 cut(s) 325, 362
FriOI GRGCYC 2 cut(s) 322, 336
GsaI CCCAGC 1 cut(s) 316
GsuI CTGGAG 2 cut(s) 96, 270
HaeIII GGCC 2 cut(s) 127, 326
HapII CCGG 1 cut(s) 379
HgaI GACGC 1 cut(s) 14
Hin1II CATG 3 cut(s) 105, 166, 208
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 209
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 190, 214, 413
Hpy188III TCNNGA 3 cut(s) 75, 244, 379
Hpy8I GTNNAC 1 cut(s) 229
HpyCH4III ACNGT 2 cut(s) 139, 404
HpyCH4IV ACGT 2 cut(s) 16, 231
HpyCH4V TGCA 2 cut(s) 37, 91
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 281
HpySE526I ACGT 2 cut(s) 16, 231
Hsp92II CATG 3 cut(s) 105, 166, 208
Kpn2I TCCGGA 1 cut(s) 378
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 185, 408
LmnI GCTCC 3 cut(s) 251, 317, 331
LweI GCATC 1 cut(s) 120
MaeII ACGT 2 cut(s) 16, 231
MaeIII GTNAC 2 cut(s) 232, 371
MalI GATC 2 cut(s) 187, 410
MboI GATC 2 cut(s) 185, 408
MboII GAAGA 1 cut(s) 149
MhlI GDGCHC 2 cut(s) 322, 336
MluCI AATT 3 cut(s) 63, 93, 118
MnlI CCTC 1 cut(s) 71
MroI TCCGGA 1 cut(s) 378
MseI TTAA 2 cut(s) 228, 290
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 2 cut(s) 223, 323
MvaI CCWGG 2 cut(s) 223, 323
MwoI GCNNNNNNNGC 2 cut(s) 59, 281
NdeII GATC 2 cut(s) 185, 408
NlaIII CATG 3 cut(s) 105, 166, 208
NlaIV GGNNCC 1 cut(s) 319
NspI RCATGY 1 cut(s) 105
OliI CACNNNNGTG 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PfeI GAWTC 1 cut(s) 209
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 221, 321
PspFI CCCAGC 1 cut(s) 312
PspGI CCWGG 2 cut(s) 221, 321
PspN4I GGNNCC 1 cut(s) 319
RsaI GTAC 3 cut(s) 87, 113, 357
RsaNI GTAC 3 cut(s) 86, 112, 356
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 228, 290
Sau3AI GATC 2 cut(s) 185, 408
ScrFI CCNGG 2 cut(s) 223, 323
SduI GDGCHC 2 cut(s) 322, 336
SetI ASST 5 cut(s) 19, 234, 256, 336, 401
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 3 cut(s) 63, 93, 118
SsiI CCGC 1 cut(s) 282
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 221, 321
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 139, 404
TaiI ACGT 2 cut(s) 19, 234
TaqI TCGA 1 cut(s) 407
TasI AATT 3 cut(s) 63, 93, 118
TatI WGTACW 3 cut(s) 85, 111, 355
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 228, 290
Tru9I TTAA 2 cut(s) 228, 290
TspDTI ATGAA 1 cut(s) 193
XapI RAATTY 1 cut(s) 93
XceI RCATGY 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.