Rh7DG243000

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008726
Physical Location & Seq
Forward (+)
25064647 .. 25069019
4373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG243000.1

Sequence Viewer

Length: 414 bp
ATGGCTGACTTACCACGCTATTTACGGCTTTGCTTTCAATCTGGGAAACTTGCTTTCTTGGCAATTTTGGTATCTGGAGGGATTGTATTGCAAATTTTGGCATGTGCTTTGTACAATAATTGGTGGCCGATGCTAACTGTAATAATGTATGTGCTTCTTCCGATGCCGTTGCTGTTCTTCGTGGGAACTGATTTGTCTGTTTTCCAGGAATCTGATAATAGCTGGGTTAATGTAACCAAGTTCATGACTGGAGCTTCAGCCATTGGAAGCATTGCAATACCAGCCATCTTAAAACACGCTGGTGTTATTGGTTGGGGAGCCCTGGCCTTGGAGCTCTCATCCTTTTTTGTATTTGTACTTGCAATAATGTGTTACATACGGTCAGATGAAGACGACAGCTACAGTTCAATCTGA

Protein Analysis

137

Amino Acids

15.08

Weight (kDa)

4.75

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 17 - 130 4e-33 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 2 cut(s) 113, 357
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 2 cut(s) 38, 408
AjnI CCWGG 2 cut(s) 204, 321
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 4 cut(s) 222, 254, 334, 399
AluI AGCT 4 cut(s) 222, 254, 334, 399
Alw21I GWGCWC 1 cut(s) 336
AoxI GGCC 2 cut(s) 125, 324
ApoI RAATTY 1 cut(s) 93
BanII GRGCYC 2 cut(s) 322, 336
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 336
BccI CCATC 1 cut(s) 293
BceAI ACGGC 2 cut(s) 41, 151
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 2 cut(s) 206, 323
BfmI CTRYAG 1 cut(s) 400
Bme1390I CCNGG 2 cut(s) 206, 323
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 2 cut(s) 206, 323
BmsI GCATC 2 cut(s) 120, 153
BpiI GAAGAC 1 cut(s) 396
BpmI CTGGAG 2 cut(s) 96, 270
BsaJI CCNNGG 2 cut(s) 321, 327
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 2 cut(s) 206, 323
BseDI CCNNGG 2 cut(s) 321, 327
BseGI GGATG 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 2 cut(s) 127, 326
BsiHKAI GWGCWC 1 cut(s) 336
BslI CCNNNNNNNGG 1 cut(s) 328
BsnI GGCC 2 cut(s) 127, 326
Bsp1286I GDGCHC 2 cut(s) 322, 336
Bsp1407I TGTACA 1 cut(s) 111
BspANI GGCC 2 cut(s) 127, 326
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 321, 327
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 206, 323
Bst4CI ACNGT 3 cut(s) 139, 381, 404
BstAUI TGTACA 1 cut(s) 111
BstF5I GGATG 1 cut(s) 338
BstMWI GCNNNNNNNGC 2 cut(s) 59, 281
BstNI CCWGG 2 cut(s) 206, 323
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 204, 321
BstSFI CTRYAG 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 127, 326
BtsCI GGATG 1 cut(s) 338
CciI TCATGA 1 cut(s) 243
Csp6I GTAC 2 cut(s) 112, 356
CviAII CATG 2 cut(s) 102, 244
CviQI GTAC 2 cut(s) 112, 356
EaeI YGGCCR 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 322, 336
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 204, 321
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 322, 336
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 105, 247
FaiI YATR 4 cut(s) 103, 150, 245, 377
FatI CATG 2 cut(s) 101, 243
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 2 cut(s) 322, 336
GsaI CCCAGC 1 cut(s) 226
GsuI CTGGAG 2 cut(s) 96, 270
HaeIII GGCC 2 cut(s) 127, 326
Hin1II CATG 2 cut(s) 105, 247
HinfI GANTC 1 cut(s) 209
Hpy188I TCNGA 4 cut(s) 162, 214, 385, 413
Hpy188III TCNNGA 2 cut(s) 75, 244
HpyCH4III ACNGT 3 cut(s) 139, 381, 404
HpyCH4V TGCA 3 cut(s) 91, 275, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 281
Hsp92II CATG 2 cut(s) 105, 247
LmnI GCTCC 3 cut(s) 251, 317, 331
LweI GCATC 2 cut(s) 120, 153
MaeIII GTNAC 2 cut(s) 232, 371
MboII GAAGA 3 cut(s) 149, 169, 401
MhlI GDGCHC 2 cut(s) 322, 336
MluCI AATT 3 cut(s) 63, 93, 118
MnlI CCTC 1 cut(s) 71
MseI TTAA 2 cut(s) 228, 290
MslI CAYNNNNRTG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 206, 323
MvaI CCWGG 2 cut(s) 206, 323
MwoI GCNNNNNNNGC 2 cut(s) 59, 281
NlaIII CATG 2 cut(s) 105, 247
NlaIV GGNNCC 1 cut(s) 319
NspI RCATGY 1 cut(s) 105
OliI CACNNNNGTG 1 cut(s) 300
PagI TCATGA 1 cut(s) 243
PfeI GAWTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 204, 321
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 2 cut(s) 204, 321
PspN4I GGNNCC 1 cut(s) 319
RsaI GTAC 2 cut(s) 113, 357
RsaNI GTAC 2 cut(s) 112, 356
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 228, 290
ScrFI CCNGG 2 cut(s) 206, 323
SduI GDGCHC 2 cut(s) 322, 336
SetI ASST 4 cut(s) 224, 256, 336, 401
SfaNI GCATC 2 cut(s) 120, 153
SfcI CTRYAG 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 3 cut(s) 63, 93, 118
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 204, 321
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 3 cut(s) 139, 381, 404
TasI AATT 3 cut(s) 63, 93, 118
TatI WGTACW 2 cut(s) 111, 355
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 2 cut(s) 228, 290
Tru9I TTAA 2 cut(s) 228, 290
TspDTI ATGAA 2 cut(s) 232, 402
XapI RAATTY 1 cut(s) 93
XceI RCATGY 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.