pycom06g03390

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008726
Physical Location & Seq
Reverse (-)
4265763 .. 4267265
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03390.1

Sequence Viewer

Length: 417 bp
ATGGCTGACTTACCAGGCTATATGCGTGCCTGCTTGCACACCGGAAAACTTGCCGCCCTGGCAATTTTGGTCTCTGGAGGGATTGTATTGCAAATTTTGGCGTGTGCTTTGTACAATAATTGGTGGCCAATGCTAACTGTAATAATGTACGTGCTTCTTCCCTTGCCATTGCTGTTCTTTGTGGGATCCGATTTTTCTATATTTTCACAATCTGAAAATGGCTGGGTAAATGCAACCAAGTTCTTGACCGGAGCTTCAGCTATTGGAAGCATTGCAATCCCTGCTATCTTAAAACACGCTGGTGTAATTGGTTGGGGGGCCCTGGCTATGGAGCTCTCATCCTTTTTCGTATTTGTACTGGCAATAATGTGTTACATTCGGACGAGTGATGATGATGACAGCTACAGTTCGATCTGA

Protein Analysis

139

Amino Acids

14.89

Weight (kDa)

4.95

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 18 - 132 9.3e-34 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 54
AclWI GGATC 2 cut(s) 180, 193
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 113, 149, 357
AfiI CCNNNNNNNGG 1 cut(s) 328
AjnI CCWGG 3 cut(s) 13, 57, 321
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 4 cut(s) 254, 260, 334, 402
AluI AGCT 4 cut(s) 254, 260, 334, 402
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 2 cut(s) 180, 193
AoxI GGCC 2 cut(s) 125, 318
ApaI GGGCCC 1 cut(s) 322
ApoI RAATTY 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 318, 319
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 322, 336
Bbv12I GWGCWC 1 cut(s) 336
BciT130I CCWGG 3 cut(s) 15, 59, 323
BcoDI GTCTC 1 cut(s) 76
BfmI CTRYAG 1 cut(s) 403
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
Bme1390I CCNGG 3 cut(s) 15, 59, 323
BmgT120I GGNCC 2 cut(s) 318, 319
BmiI GGNNCC 3 cut(s) 187, 319, 320
BmrFI CCNGG 3 cut(s) 15, 59, 323
BpmI CTGGAG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 151
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 57, 321
BsaWI WCCGGW 2 cut(s) 41, 248
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 1 cut(s) 363
Bse3DI GCAATG 2 cut(s) 167, 270
BseBI CCWGG 3 cut(s) 15, 59, 323
BseDI CCNNGG 2 cut(s) 57, 321
BseGI GGATG 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 2 cut(s) 167, 270
BseNI ACTGG 1 cut(s) 363
BseSI GKGCMC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 2 cut(s) 127, 320
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 2 cut(s) 42, 249
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 76
BsnI GGCC 2 cut(s) 127, 320
Bso31I GGTCTC 1 cut(s) 76
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 2 cut(s) 322, 336
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 185, 411
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 127, 320
BspLI GGNNCC 3 cut(s) 187, 319, 320
BspPI GGATC 2 cut(s) 180, 193
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 2 cut(s) 167, 270
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 2 cut(s) 57, 321
BssMI GATC 2 cut(s) 185, 411
Bst2UI CCWGG 3 cut(s) 15, 59, 323
Bst4CI ACNGT 2 cut(s) 139, 407
BstAPI GCANNNNNTGC 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 27, 31, 35
BstF5I GGATG 1 cut(s) 338
BstKTI GATC 2 cut(s) 188, 414
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 185, 411
BstMWI GCNNNNNNNGC 2 cut(s) 59, 281
BstNI CCWGG 3 cut(s) 15, 59, 323
BstSCI CCNGG 3 cut(s) 13, 57, 321
BstSFI CTRYAG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 2 cut(s) 127, 320
BtsCI GGATG 1 cut(s) 338
Cac8I GCNNGC 3 cut(s) 27, 31, 35
Cfr13I GGNCC 2 cut(s) 318, 319
Csp6I GTAC 3 cut(s) 112, 148, 356
CviQI GTAC 3 cut(s) 112, 148, 356
DpnI GATC 2 cut(s) 187, 413
DpnII GATC 2 cut(s) 185, 411
EaeI YGGCCR 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 2 cut(s) 322, 336
Eco31I GGTCTC 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 318, 319
EcoRII CCWGG 3 cut(s) 13, 57, 321
EcoT38I GRGCYC 2 cut(s) 322, 336
FaiI YATR 4 cut(s) 21, 23, 200, 329
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 2 cut(s) 322, 336
Fsp4HI GCNGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 54
GsaI CCCAGC 1 cut(s) 226
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 127, 320
HapII CCGG 2 cut(s) 42, 249
HpaII CCGG 2 cut(s) 42, 249
Hpy188I TCNGA 4 cut(s) 190, 214, 381, 416
Hpy188III TCNNGA 2 cut(s) 75, 244
HpyCH4III ACNGT 2 cut(s) 139, 407
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 37, 91, 233, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 281
HpySE526I ACGT 1 cut(s) 150
Kzo9I GATC 2 cut(s) 185, 411
LmnI GCTCC 2 cut(s) 251, 331
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 371
MalI GATC 2 cut(s) 187, 413
MboI GATC 2 cut(s) 185, 411
MboII GAAGA 1 cut(s) 149
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 2 cut(s) 322, 336
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 4 cut(s) 63, 93, 118, 306
MluNI TGGCCA 1 cut(s) 127
MnlI CCTC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 127
MseI TTAA 1 cut(s) 290
MslI CAYNNNNRTG 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 127
MspI CCGG 2 cut(s) 42, 249
MspR9I CCNGG 3 cut(s) 15, 59, 323
MvaI CCWGG 3 cut(s) 15, 59, 323
MwoI GCNNNNNNNGC 2 cut(s) 59, 281
NdeII GATC 2 cut(s) 185, 411
NlaIV GGNNCC 3 cut(s) 187, 319, 320
OliI CACNNNNGTG 1 cut(s) 300
PkrI GCNGC 1 cut(s) 55
Ppu21I YACGTR 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 13, 57, 321
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 3 cut(s) 13, 57, 321
PspN4I GGNNCC 3 cut(s) 187, 319, 320
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 2 cut(s) 318, 319
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 3 cut(s) 113, 149, 357
RsaNI GTAC 3 cut(s) 112, 148, 356
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 185, 411
Sau96I GGNCC 2 cut(s) 318, 319
ScrFI CCNGG 3 cut(s) 15, 59, 323
SduI GDGCHC 2 cut(s) 322, 336
SetI ASST 5 cut(s) 153, 256, 262, 336, 404
SfcI CTRYAG 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 4 cut(s) 63, 93, 118, 306
SsiI CCGC 1 cut(s) 54
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 3 cut(s) 13, 57, 321
TaaI ACNGT 2 cut(s) 139, 407
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 410
TasI AATT 4 cut(s) 63, 93, 118, 306
TatI WGTACW 2 cut(s) 111, 355
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
XapI RAATTY 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.