RLG00000003233

Vacuolar protein sorting-associated protein 55 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008726
Physical Location & Seq
Reverse (-)
45902618 .. 45906263
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003233

Sequence Viewer

Length: 336 bp
ATGTTATGTGTTTTGAGGCAGGCATGTGCTTTGTACAATAATTGGTGGCCGATGCTAACTGTAATAATGTATGTGCTTCTTCCGATGCCGTTGCTGTTCTTTGTGGGAACTGATTTATCTGTTTTCCAGGATTCTGATAATAGCTGGGTTAATGTAACCAAGTTCATGACTGGAGCTTCAGCCATTGGAAGCATTGCAATACCAGCCATCTTAAAACACGCTGGTGTTATTGGTTGGGGAGCCCTGGCTTTGGAGCTCTCATCCTTTTTCGTATTTGTACTCGCAATAATGTGTTACATACGGTCAGATGATGACGACAGCTACAGTTCAATCTGA

Protein Analysis

112

Amino Acids

12.29

Weight (kDa)

4.35

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps55 PF04133 8 - 104 5.7e-28 Vacuolar protein sorting 55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 47
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 2 cut(s) 35, 279
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 1 cut(s) 330
AjnI CCWGG 2 cut(s) 126, 243
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 4 cut(s) 144, 176, 256, 321
AluI AGCT 4 cut(s) 144, 176, 256, 321
Alw21I GWGCWC 1 cut(s) 258
AoxI GGCC 1 cut(s) 47
BanII GRGCYC 2 cut(s) 244, 258
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 2 cut(s) 128, 245
BfmI CTRYAG 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 128, 245
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 2 cut(s) 128, 245
BmsI GCATC 2 cut(s) 42, 75
BpmI CTGGAG 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 2 cut(s) 128, 245
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 250
BsnI GGCC 1 cut(s) 49
Bsp1286I GDGCHC 2 cut(s) 244, 258
Bsp1407I TGTACA 1 cut(s) 33
BspANI GGCC 1 cut(s) 49
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 192
BsrGI TGTACA 1 cut(s) 33
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 128, 245
Bst4CI ACNGT 3 cut(s) 61, 303, 326
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 21
BstF5I GGATG 1 cut(s) 260
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstNI CCWGG 2 cut(s) 128, 245
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 2 cut(s) 126, 243
BstSFI CTRYAG 1 cut(s) 322
BsuRI GGCC 1 cut(s) 49
BtsCI GGATG 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 21
CciI TCATGA 1 cut(s) 165
Csp6I GTAC 2 cut(s) 34, 278
CviAII CATG 2 cut(s) 24, 166
CviJI RGCY 9 cut(s) 49, 144, 176, 182, 206, 242, 248, 256, 321
CviKI_1 RGCY 9 cut(s) 49, 144, 176, 182, 206, 242, 248, 256, 321
CviQI GTAC 2 cut(s) 34, 278
EaeI YGGCCR 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 256
Eco24I GRGCYC 2 cut(s) 244, 258
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 126, 243
EcoT38I GRGCYC 2 cut(s) 244, 258
FaeI CATG 2 cut(s) 27, 169
FaiI YATR 5 cut(s) 7, 25, 72, 167, 299
FatI CATG 2 cut(s) 23, 165
FokI GGATG 1 cut(s) 247
FriOI GRGCYC 2 cut(s) 244, 258
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 1 cut(s) 49
Hin1II CATG 2 cut(s) 27, 169
HinfI GANTC 1 cut(s) 131
Hpy188I TCNGA 4 cut(s) 84, 136, 307, 335
Hpy188III TCNNGA 1 cut(s) 166
HpyCH4III ACNGT 3 cut(s) 61, 303, 326
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
Hsp92II CATG 2 cut(s) 27, 169
LmnI GCTCC 3 cut(s) 173, 239, 253
LpnPI CCDG 9 cut(s) 5, 113, 130, 140, 156, 207, 216, 230, 257
LweI GCATC 2 cut(s) 42, 75
MaeIII GTNAC 2 cut(s) 154, 293
MboII GAAGA 1 cut(s) 71
MhlI GDGCHC 2 cut(s) 244, 258
MluCI AATT 1 cut(s) 40
MnlI CCTC 1 cut(s) 9
MseI TTAA 2 cut(s) 150, 212
MslI CAYNNNNRTG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 128, 245
MvaI CCWGG 2 cut(s) 128, 245
MwoI GCNNNNNNNGC 1 cut(s) 203
NlaIII CATG 2 cut(s) 27, 169
NlaIV GGNNCC 1 cut(s) 241
NspI RCATGY 1 cut(s) 27
OliI CACNNNNGTG 1 cut(s) 222
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 126, 243
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 2 cut(s) 126, 243
PspN4I GGNNCC 1 cut(s) 241
RsaI GTAC 2 cut(s) 35, 279
RsaNI GTAC 2 cut(s) 34, 278
RseI CAYNNNNRTG 1 cut(s) 222
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 150, 212
ScrFI CCNGG 2 cut(s) 128, 245
SduI GDGCHC 2 cut(s) 244, 258
SetI ASST 4 cut(s) 146, 178, 258, 323
SfaNI GCATC 2 cut(s) 42, 75
SfcI CTRYAG 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 1 cut(s) 40
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 126, 243
TaaI ACNGT 3 cut(s) 61, 303, 326
TasI AATT 1 cut(s) 40
TatI WGTACW 2 cut(s) 33, 277
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 2 cut(s) 150, 212
Tru9I TTAA 2 cut(s) 150, 212
TspDTI ATGAA 1 cut(s) 154
XceI RCATGY 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.