AT1G32810

Zinc ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013452
Physical Location & Seq
Forward (+)
11881311 .. 11886015
4705 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32810.2

Sequence Viewer

Length: 3207 bp
ATGAAAGGAAGATCGTATCGGTTTTCAAGTACGGATCCTCATGAGGATTGGGTTGATGGACTTTGGACTGTGGATTGTGTTTGTGGTGTGAATGACGATGATGGAACAGAGATGGTTAAATGTGATGATTGTGGTGTTTGGGTTCATACTCGTTGTTCTCGTTTTGTTGAAGGACAAGAGCTTTTTACTTGTCATAAGTGTAAGAGCAAGAACAATGTTAATGATAGTGAAGAGACTGAGGTTGCTCAGTTGTTGGTTGAATTGCCTACTAAGACTTTGGGAATGGAGAATTCGTGTACTCGGAGTGTACCTTTCAAGCGTCCTTTTAGGTTATGGACTGAGATCCCAGCTGAAGAAAAGGTTCATGTGCAAGGAATCCCGGGTGGGGATCCGTCTCTTTTTGATGGATTGTCGTCGGTTTTCTCCCGGGAGCTGTGGAAGTGTAGTGGTTATGTGCCTAAGAAGTTTAACCTTAAGTATAGAGAGTTTCCTTGCTGGGATGAGCAAGAGAAGGATGAAGACGGTGCAGGGGTTTTGTTTTCGATGTCGAAGGAGAATGTGATTGCTGCTCCTGTTAGTACTTTGGTTGGAATGAGGAGGAGTCTTGATGGTAAAGGAGGAACCAATGACGTGAAGCTTGGTTGTGACTCTGGTGAAACGGATAGAAAACATTCTCAGGGTGCTATTAAGAAGGATAAGAGGTTGCTTCGTCCTATGATGACGAATAAACGAAGAAAAGAATTGTTCGGTGCTTCAAAAGAGCGGATGAAGAAGAAGGTTGAAGTTGTAGACAAAGAAGAAGATGATAAGAAAGGTTTTGTCGGTAAAACAGGAAATCGACCTGCCAGTGATGCTAAACCATCAGAATCTCGTAAGGACATAGAAGCAGAAGGTTTTACTAGCGACGTTGGGATCACGAAGAGTGTAAAGGCCAAGAAAGCTGCTCTGGAAACTGGCGGTGATGAATCTGGTAATACAGAAATTGGTGTTGAATGCTCCAGAGAACAGAATCTTTCTGACGTTCATGCTAATGGTACTGGAAAACAGGAAGAGAAAGCTGGTCATCATTTCCGAATTGTGCTTAAAAGCTCTGCAACTACTGATCCTTCTGTCCTCGGAGGAAGAGATGTTCCACACAATGAGGCTAATAAGGAGGAGGAGAGACAAGGCACAATAGCTGATGCTCCAGAAGATAATGCAGCTGATTCTTCTGAAAGTTCCCAGAAGCCTTCTCTTGGTTCCATGGTTGGTAAAACACGAGAAGGTGAAGAGAAAAATTGTGATGATGTTAGTAGGAAAATCAGTACGAGGAAGAACAAATTCCAAAAAGAAACAGCTGATACTGGTGCCAGTGGAGCTCTAGGCCTGCAGACTCTAGACCATATGGATTCTAAAGTGTCAGGGAGCTCTGCGTCTCAGATCTCTGGAGGTTCTGAACTTAACAAAATGACACCGAGCAGTTCACTTCCTGATGATCACAAACCGCAGTCTGTTGAAATGGTATCAGAGGGAATAAGTAGTGGCAATAGAGACAGAGCTATCGAATTAAAACGAGAACTGGTGGTTTCAGAAACTGAAAAAGATATTCAGGAAACAAAGCCTGGATCTGTTTTATTTCAAGAGCCATCAAAGCCTTGCAGACCTATTCCGCATACGGTTTCAGGAAATGGTAGGCCTAAGATGGTAGTATGCATTGGAAAAACATCGTCAAGTTCAGCCACTGAAAAGTCCCCTAAGCCCTCAACTTCCAGGAATTCTATTCCTGGCTTGAAGCAACAACCAGGTGATGATGATAATGATGCTAATACCAATGATGAGGACTGTGTTTCCAGTGATGTGATCCGAGAGAGAGATGGGGATGATGAACCTTCAGAGAAAGCTCCGAAACATCCTAAATTTTCTATTACCTCTAAGAAATCGATGCAGCATAACCGTACTTCCCATTCTTCAGTTTCCAAGACAAGAGAGTCTTCCTCTTCTTCAAAGACATCTTCAGCAACTCGGATTAATGGTGGTTCTTCTGAGGCTCCTAGTAAACATTCGCTTTCAGGAACCTTCCCGAAAAATGAAAAGCCTGGACAATCAATATTCCAGTCATCAACTAAGAACCCTGTGCAGTCTATAATTTCCCTTGCTCCAAATCTGAGCGATGAAGAGCTTGCGTTAAGACTGCACCATCAACTCAATAGTTCTCCTCGAGTTCCTCGTGTCCCCCGCATGAGACAGCCTGGTAGCTTGCCCCTGTCTCCAACCGCCCCTAGTTTTAAGCGCACTTCTAGTTCTGGTAGCAAGGATCACACAACGTTTTCCAGAAGGAAAAACAAGGATACATCCAAAGAAGGTTACTGCAATTTACGTGATGATGATAGATGCAGTACAAGGAGTGCCAAAAATCGTCGGTCTCCTGATAGGAGGACACAACAAGACAGTGGAAGCAGAGGAGGGAGTTTGTGTTCCAAAGGGGAAGAAAATGAAACCACCAAAACCTCTTCTTATTCCTCTCGGAAAGTACTTCTCCCGCCAAACTCGACTACAAGCACAAGCAGCGGACCATGTTCATCCAGCGAGTTGAATGAGCATAATAAGCCTTCTCCACACAGTTCCCCTAGGAATAATGGTACTCCAGTACATCGAACTTTGCCAGGATTAATCAATGAAATCATGAACAAAGGCAAGAGGATGGCATATGAGGAGCTCTGTAACGCAGTCTTGCCGCACTGGCCTCACTTGAGGAAACACAACGGAGAACGATATGCATATTCAAGCCATTCACAGGCTGTTCTTGATTGCCTGAGGAATAGGCATGAGTGGGCTCGTCTGGTTGATCGTGGCCCCAAGACTAATTCCGGGAAAAAGAAACGGAAGCTCGATGCAGCAGAGGAGGATTCAGATGAGAACGAAAGCAGTAAGGGAGGAAGGAAGCGACTGCATCAACATCACTCTCAAGGAGAAGAGTTTCCCAAAGGAAAAAGGAAAGCAAGAAAACGGAGAAGGCTTTCTATTCAAAGAAAAGGCATTAAAGTGTTGAGAAAGAAGAGAAACGAAGAAGAAGTTAGCGAAGAAGACGAGGAAGATGCGTTCTCGGACACAAGCGAGGAGAGCATCTTCTGCGATGAAGAAGAAGAAGAAGAAGAAGAGGGCCACACTGCTACTGGTACTGGACAAGTCTCTGCTAGTAGCTCTGAGGAAGCTCAAGCCACTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1068

Amino Acids

117.55

Weight (kDa)

8.03

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 879 - 941 3.3e-33 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1966
Acc36I ACCTGC 1 cut(s) 850
AccB1I GGYRCC 1 cut(s) 1346
AccBSI CCGCTC 1 cut(s) 763
AccI GTMKAC 1 cut(s) 789
AciI CCGC 9 cut(s) 763, 957, 1484, 1649, 2215, 2253, 2519, 2547, 2714
AclI AACGTT 1 cut(s) 2303
AcsI RAATTY 4 cut(s) 289, 1319, 1753, 1895
AcuI CTGAAG 4 cut(s) 372, 1854, 1932, 1977
AfiI CCNNNNNNNGG 3 cut(s) 385, 1235, 2773
AflII CTTAAG 1 cut(s) 473
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 7 cut(s) 1600, 1748, 1762, 1780, 2074, 2227, 2641
AloI GAACNNNNNNTCC 6 cut(s) 1113, 1145, 2437, 2469, 3062, 3094
Alw21I GWGCWC 3 cut(s) 1360, 1409, 2697
Alw26I GTCTC 9 cut(s) 227, 399, 1156, 1419, 1524, 2215, 2250, 2406, 3172
AlwNI CAGNNNCTG 2 cut(s) 1574, 1721
Ama87I CYCGRG 3 cut(s) 379, 426, 2196
AoxI GGCC 6 cut(s) 930, 1363, 1673, 2720, 2830, 3139
ApeKI GCWGC 6 cut(s) 566, 941, 1199, 1924, 2544, 2873
ApoI RAATTY 4 cut(s) 289, 1319, 1753, 1895
AseI ATTAAT 2 cut(s) 2007, 2648
Asp700I GAANNNNTTC 5 cut(s) 360, 670, 1319, 2955, 2995
AspA2I CCTAGG 1 cut(s) 2606
AspLEI GCGC 1 cut(s) 2271
AspS9I GGNCC 3 cut(s) 2549, 2831, 3139
AsuC2I CCSGG 5 cut(s) 380, 381, 427, 428, 2848
AsuHPI GGTGA 4 cut(s) 665, 971, 1277, 1796
AvaI CYCGRG 3 cut(s) 379, 426, 2196
AvaII GGWCC 1 cut(s) 2549
AvrII CCTAGG 1 cut(s) 2606
AxyI CCTNAGG 1 cut(s) 2792
BaeI ACNNNNGTAYC 2 cut(s) 2610, 2643
BamHI GGATCC 2 cut(s) 34, 388
BanI GGYRCC 1 cut(s) 1346
BanII GRGCYC 4 cut(s) 1360, 1409, 2697, 2815
BauI CACGAG 2 cut(s) 1257, 2205
BbsI GAAGAC 3 cut(s) 525, 1962, 3069
Bbv12I GWGCWC 3 cut(s) 1360, 1409, 2697
BbvI GCAGC 6 cut(s) 553, 928, 1211, 1936, 2556, 2885
BcgI CGANNNNNNTGC 2 cut(s) 3056, 3090
BciT130I CCWGG 7 cut(s) 1602, 1750, 1764, 1782, 2076, 2229, 2643
BciVI GTATCC 1 cut(s) 2320
BclI TGATCA 1 cut(s) 1474
BcnI CCSGG 5 cut(s) 380, 381, 427, 428, 2848
BcoDI GTCTC 9 cut(s) 227, 399, 1156, 1419, 1524, 2215, 2250, 2406, 3172
BfaI CTAG 8 cut(s) 900, 1361, 1376, 2031, 2259, 2277, 2607, 3174
BfmI CTRYAG 1 cut(s) 1367
BfrI CTTAAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 850
BfuI GTATCC 1 cut(s) 2320
BglI GCCNNNNNGGC 1 cut(s) 2719
BglII AGATCT 1 cut(s) 1419
BisI GCNGC 7 cut(s) 567, 942, 1200, 1925, 2545, 2714, 2874
BlnI CCTAGG 1 cut(s) 2606
BlsI GCNGC 7 cut(s) 568, 943, 1201, 1926, 2546, 2715, 2875
BmcAI AGTACT 2 cut(s) 580, 2511
Bme18I GGWCC 1 cut(s) 2549
BmeT110I CYCGRG 3 cut(s) 379, 426, 2196
BmgBI CACGTC 1 cut(s) 631
BmgT120I GGNCC 3 cut(s) 2549, 2831, 3139
BmiI GGNNCC 8 cut(s) 36, 390, 622, 1240, 1348, 2028, 2053, 2833
BmsI GCATC 9 cut(s) 841, 1171, 1789, 1911, 2360, 2860, 2938, 3064, 3111
BpiI GAAGAC 3 cut(s) 525, 1962, 3069
BplI GAGNNNNNCTC 2 cut(s) 1958, 1990
BpmI CTGGAG 4 cut(s) 982, 1170, 1446, 2607
Bpu10I CCTNAGC 1 cut(s) 1734
BpuEI CTTGAG 3 cut(s) 2749, 2928, 3177
BpuMI CCSGG 5 cut(s) 380, 381, 427, 428, 2848
Bsa29I ATCGAT 1 cut(s) 1919
BsaAI YACGTR 1 cut(s) 2357
BsaI GGTCTC 1 cut(s) 2406
BsaJI CCNNGG 5 cut(s) 379, 426, 1114, 1242, 2606
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 2437, 2467
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 1235, 2773
Bse21I CCTNAGG 1 cut(s) 2792
BseBI CCWGG 7 cut(s) 1602, 1750, 1764, 1782, 2076, 2229, 2643
BseCI ATCGAT 1 cut(s) 1919
BseDI CCNNGG 5 cut(s) 379, 426, 1114, 1242, 2606
BseGI GGATG 8 cut(s) 505, 520, 771, 1864, 1888, 2330, 2558, 2685
BseLI CCNNNNNNNGG 3 cut(s) 385, 1235, 2773
BseMII CTCAG 9 cut(s) 228, 260, 330, 689, 1430, 2013, 2135, 2783, 3174
BseRI GAGGAG 9 cut(s) 610, 613, 1169, 1172, 2184, 2454, 2705, 2894, 3110
BseXI GCAGC 6 cut(s) 553, 928, 1211, 1936, 2556, 2885
BseYI CCCAGC 2 cut(s) 346, 495
BsgI GTGCAG 3 cut(s) 546, 2135, 2156
BshFI GGCC 6 cut(s) 932, 1365, 1675, 2722, 2832, 3141
BshNI GGYRCC 1 cut(s) 1346
BshVI ATCGAT 1 cut(s) 1919
BsiHKAI GWGCWC 3 cut(s) 1360, 1409, 2697
BsiHKCI CYCGRG 3 cut(s) 379, 426, 2196
BsiSI CCGG 3 cut(s) 380, 427, 2847
BslFI GGGAC 2 cut(s) 1714, 2195
BslI CCNNNNNNNGG 3 cut(s) 385, 1235, 2773
BsmAI GTCTC 9 cut(s) 227, 399, 1156, 1419, 1524, 2215, 2250, 2406, 3172
BsmBI CGTCTC 2 cut(s) 399, 1419
BsmFI GGGAC 2 cut(s) 1714, 2195
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 6 cut(s) 932, 1365, 1675, 2722, 2832, 3141
Bso31I GGTCTC 1 cut(s) 2406
BsoBI CYCGRG 3 cut(s) 379, 426, 2196
Bsp1286I GDGCHC 4 cut(s) 1360, 1409, 2697, 2815
Bsp19I CCATGG 1 cut(s) 1242
BspACI CCGC 9 cut(s) 763, 957, 1484, 1649, 2215, 2253, 2519, 2547, 2714
BspANI GGCC 6 cut(s) 932, 1365, 1675, 2722, 2832, 3141
BspCNI CTCAG 9 cut(s) 229, 259, 331, 688, 1429, 2014, 2136, 2784, 3175
BspDI ATCGAT 1 cut(s) 1919
BspHI TCATGA 3 cut(s) 40, 2661, 3203
BspLI GGNNCC 8 cut(s) 36, 390, 622, 1240, 1348, 2028, 2053, 2833
BspMAI CTGCAG 1 cut(s) 1371
BspMI ACCTGC 1 cut(s) 850
BspQI GCTCTTC 1 cut(s) 2148
BspT107I GGYRCC 1 cut(s) 1346
BspTI CTTAAG 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 2406
BsrBI CCGCTC 1 cut(s) 763
BssECI CCNNGG 5 cut(s) 379, 426, 1114, 1242, 2606
BssSI CACGAG 2 cut(s) 1257, 2205
BssT1I CCWWGG 2 cut(s) 1242, 2606
Bst2BI CACGAG 2 cut(s) 1257, 2205
Bst2UI CCWGG 7 cut(s) 1602, 1750, 1764, 1782, 2076, 2229, 2643
Bst4CI ACNGT 7 cut(s) 70, 524, 1657, 1823, 1934, 2429, 2600
BstAFI CTTAAG 1 cut(s) 473
BstAPI GCANNNNNTGC 1 cut(s) 3108
BstBAI YACGTR 1 cut(s) 2357
BstC8I GCNNGC 3 cut(s) 1367, 2160, 2237
BstDSI CCRYGG 1 cut(s) 1242
BstF5I GGATG 8 cut(s) 505, 520, 771, 1864, 1888, 2330, 2558, 2685
BstHHI GCGC 1 cut(s) 2271
BstMAI GTCTC 9 cut(s) 227, 399, 1156, 1419, 1524, 2215, 2250, 2406, 3172
BstMWI GCNNNNNNNGC 9 cut(s) 851, 938, 1355, 1630, 2544, 2584, 2719, 3099, 3108
BstNI CCWGG 7 cut(s) 1602, 1750, 1764, 1782, 2076, 2229, 2643
BstSFI CTRYAG 1 cut(s) 1367
BstV1I GCAGC 6 cut(s) 553, 928, 1211, 1936, 2556, 2885
BstV2I GAAGAC 3 cut(s) 525, 1962, 3069
BstX2I RGATCY 5 cut(s) 34, 342, 388, 1419, 1604
BstYI RGATCY 5 cut(s) 34, 342, 388, 1419, 1604
Bsu15I ATCGAT 1 cut(s) 1919
Bsu36I CCTNAGG 1 cut(s) 2792
BsuI GTATCC 1 cut(s) 2320
BsuRI GGCC 6 cut(s) 932, 1365, 1675, 2722, 2832, 3141
BsuTUI ATCGAT 1 cut(s) 1919
BtgI CCRYGG 1 cut(s) 1242
BtgZI GCGATG 2 cut(s) 2163, 3126
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 8 cut(s) 505, 520, 771, 1864, 1888, 2330, 2558, 2685
BtsI GCAGTG 1 cut(s) 3144
BtsIMutI CAGTG 7 cut(s) 853, 1357, 1719, 1837, 2434, 2716, 3144
BveI ACCTGC 1 cut(s) 850
Cac8I GCNNGC 3 cut(s) 1367, 2160, 2237
CaiI CAGNNNCTG 2 cut(s) 1574, 1721
CciI TCATGA 3 cut(s) 40, 2661, 3203
CfoI GCGC 1 cut(s) 2271
Cfr13I GGNCC 3 cut(s) 2549, 2831, 3139
Cfr9I CCCGGG 2 cut(s) 379, 426
ClaI ATCGAT 1 cut(s) 1919
CseI GACGC 2 cut(s) 308, 1401
CsiI ACCWGGT 1 cut(s) 1780
CviAII CATG 9 cut(s) 41, 365, 1025, 1243, 2218, 2553, 2662, 2804, 3204
DrdI GACNNNNNNGTC 1 cut(s) 1966
DseDI GACNNNNNNGTC 1 cut(s) 1966
Ecl136II GAGCTC 3 cut(s) 1358, 1407, 2695
Eco130I CCWWGG 2 cut(s) 1242, 2606
Eco147I AGGCCT 2 cut(s) 1365, 1675
Eco24I GRGCYC 4 cut(s) 1360, 1409, 2697, 2815
Eco31I GGTCTC 1 cut(s) 2406
Eco47I GGWCC 1 cut(s) 2549
Eco53kI GAGCTC 3 cut(s) 1358, 1407, 2695
Eco57I CTGAAG 4 cut(s) 372, 1854, 1932, 1977
Eco81I CCTNAGG 1 cut(s) 2792
Eco88I CYCGRG 3 cut(s) 379, 426, 2196
EcoICRI GAGCTC 3 cut(s) 1358, 1407, 2695
EcoRI GAATTC 2 cut(s) 289, 1753
EcoRII CCWGG 7 cut(s) 1600, 1748, 1762, 1780, 2074, 2227, 2641
EcoT14I CCWWGG 2 cut(s) 1242, 2606
EcoT22I ATGCAT 2 cut(s) 1694, 2758
EcoT38I GRGCYC 4 cut(s) 1360, 1409, 2697, 2815
ErhI CCWWGG 2 cut(s) 1242, 2606
Esp3I CGTCTC 2 cut(s) 399, 1419
FaeI CATG 9 cut(s) 44, 368, 1028, 1246, 2221, 2556, 2665, 2807, 3207
FalI AAGNNNNNCTT 2 cut(s) 1954, 1986
FaqI GGGAC 2 cut(s) 1714, 2195
FatI CATG 9 cut(s) 40, 364, 1024, 1242, 2217, 2552, 2661, 2803, 3203
FauI CCCGC 2 cut(s) 2222, 2526
FauNDI CATATG 2 cut(s) 1383, 2686
FbaI TGATCA 1 cut(s) 1474
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 7 cut(s) 567, 942, 1200, 1925, 2545, 2714, 2874
FokI GGATG 8 cut(s) 512, 527, 778, 1871, 1875, 2317, 2545, 2692
FriOI GRGCYC 4 cut(s) 1360, 1409, 2697, 2815
Fsp4HI GCNGC 7 cut(s) 567, 942, 1200, 1925, 2545, 2714, 2874
FspBI CTAG 8 cut(s) 900, 1361, 1376, 2031, 2259, 2277, 2607, 3174
GlaI GCGC 1 cut(s) 2270
GluI GCNGC 7 cut(s) 567, 942, 1200, 1925, 2545, 2714, 2874
GsaI CCCAGC 2 cut(s) 350, 499
GsuI CTGGAG 4 cut(s) 982, 1170, 1446, 2607
HaeIII GGCC 6 cut(s) 932, 1365, 1675, 2722, 2832, 3141
HapII CCGG 3 cut(s) 380, 427, 2847
HgaI GACGC 2 cut(s) 308, 1401
HhaI GCGC 1 cut(s) 2271
Hin1II CATG 9 cut(s) 44, 368, 1028, 1246, 2221, 2556, 2665, 2807, 3207
Hin6I GCGC 1 cut(s) 2269
HinP1I GCGC 1 cut(s) 2269
HindIII AAGCTT 1 cut(s) 635
HpaII CCGG 3 cut(s) 380, 427, 2847
HphI GGTGA 4 cut(s) 665, 971, 1277, 1796
Hpy166II GTNNAC 5 cut(s) 297, 308, 790, 1463, 2036
Hpy8I GTNNAC 5 cut(s) 297, 308, 790, 1463, 2036
Hpy99I CGWCG 3 cut(s) 418, 908, 2400
HpyCH4III ACNGT 7 cut(s) 70, 524, 1657, 1823, 1934, 2429, 2600
HpyCH4IV ACGT 5 cut(s) 630, 906, 1020, 2303, 2356
HpyF10VI GCNNNNNNNGC 9 cut(s) 851, 938, 1355, 1630, 2544, 2584, 2719, 3099, 3108
HpySE526I ACGT 5 cut(s) 630, 906, 1020, 2303, 2356
Hsp92II CATG 9 cut(s) 44, 368, 1028, 1246, 2221, 2556, 2665, 2807, 3207
HspAI GCGC 1 cut(s) 2269
Ksp22I TGATCA 1 cut(s) 1474
LguI GCTCTTC 1 cut(s) 2148
Lsp1109I GCAGC 6 cut(s) 553, 928, 1211, 1936, 2556, 2885
LweI GCATC 9 cut(s) 841, 1171, 1789, 1911, 2360, 2860, 2938, 3064, 3111
MabI ACCWGGT 1 cut(s) 1780
MaeI CTAG 8 cut(s) 900, 1361, 1376, 2031, 2259, 2277, 2607, 3174
MaeII ACGT 5 cut(s) 630, 906, 1020, 2303, 2356
MaeIII GTNAC 3 cut(s) 644, 2342, 2699
MbiI CCGCTC 1 cut(s) 763
MflI RGATCY 5 cut(s) 34, 342, 388, 1419, 1604
MhlI GDGCHC 4 cut(s) 1360, 1409, 2697, 2815
MlyI GAGTC 4 cut(s) 610, 641, 1366, 1976
MmeI TCCRAC 2 cut(s) 568, 2273
Mph1103I ATGCAT 2 cut(s) 1694, 2758
MroXI GAANNNNTTC 5 cut(s) 360, 670, 1319, 2955, 2995
MslI CAYNNNNRTG 2 cut(s) 1029, 3020
MspA1I CMGCKG 4 cut(s) 350, 1202, 1337, 2547
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 3 cut(s) 380, 427, 2847
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 7 cut(s) 1602, 1750, 1764, 1782, 2076, 2229, 2643
MwoI GCNNNNNNNGC 9 cut(s) 851, 938, 1355, 1630, 2544, 2584, 2719, 3099, 3108
NciI CCSGG 5 cut(s) 380, 381, 427, 428, 2848
NcoI CCATGG 1 cut(s) 1242
NdeI CATATG 2 cut(s) 1383, 2686
NlaIII CATG 9 cut(s) 44, 368, 1028, 1246, 2221, 2556, 2665, 2807, 3207
NlaIV GGNNCC 8 cut(s) 36, 390, 622, 1240, 1348, 2028, 2053, 2833
NmuCI GTSAC 1 cut(s) 644
NsiI ATGCAT 2 cut(s) 1694, 2758
PaeR7I CTCGAG 1 cut(s) 2196
PagI TCATGA 3 cut(s) 40, 2661, 3203
PceI AGGCCT 2 cut(s) 1365, 1675
PciSI GCTCTTC 1 cut(s) 2148
PcsI WCGNNNNNNNCGW 2 cut(s) 157, 2203
PctI GAATGC 1 cut(s) 998
PdmI GAANNNNTTC 5 cut(s) 360, 670, 1319, 2955, 2995
PfeI GAWTC 7 cut(s) 375, 866, 965, 1009, 1205, 1388, 2885
PfoI TCCNGGA 2 cut(s) 1748, 2846
PkrI GCNGC 7 cut(s) 568, 943, 1201, 1926, 2546, 2715, 2875
PleI GAGTC 4 cut(s) 609, 641, 1366, 1975
PpsI GAGTC 4 cut(s) 609, 641, 1366, 1975
Ppu21I YACGTR 1 cut(s) 2357
PshBI ATTAAT 2 cut(s) 2007, 2648
Psp124BI GAGCTC 3 cut(s) 1360, 1409, 2697
Psp1406I AACGTT 1 cut(s) 2303
Psp6I CCWGG 7 cut(s) 1600, 1748, 1762, 1780, 2074, 2227, 2641
PspFI CCCAGC 2 cut(s) 346, 495
PspGI CCWGG 7 cut(s) 1600, 1748, 1762, 1780, 2074, 2227, 2641
PspN4I GGNNCC 8 cut(s) 36, 390, 622, 1240, 1348, 2028, 2053, 2833
PspPI GGNCC 3 cut(s) 2549, 2831, 3139
PspXI VCTCGAGB 1 cut(s) 2196
PstI CTGCAG 1 cut(s) 1371
PstNI CAGNNNCTG 2 cut(s) 1574, 1721
PsuI RGATCY 5 cut(s) 34, 342, 388, 1419, 1604
PvuII CAGCTG 3 cut(s) 350, 1202, 1337
RseI CAYNNNNRTG 2 cut(s) 1029, 3020
SacI GAGCTC 3 cut(s) 1360, 1409, 2697
SapI GCTCTTC 1 cut(s) 2148
SatI GCNGC 7 cut(s) 567, 942, 1200, 1925, 2545, 2714, 2874
Sau96I GGNCC 3 cut(s) 2549, 2831, 3139
ScaI AGTACT 2 cut(s) 580, 2511
SchI GAGTC 4 cut(s) 610, 641, 1366, 1976
SduI GDGCHC 4 cut(s) 1360, 1409, 2697, 2815
SexAI ACCWGGT 1 cut(s) 1780
SfaNI GCATC 9 cut(s) 841, 1171, 1789, 1911, 2360, 2860, 2938, 3064, 3111
SfcI CTRYAG 1 cut(s) 1367
Sfr274I CTCGAG 1 cut(s) 2196
SinI GGWCC 1 cut(s) 2549
SlaI CTCGAG 1 cut(s) 2196
SmaI CCCGGG 2 cut(s) 381, 428
SmiMI CAYNNNNRTG 2 cut(s) 1029, 3020
SmlI CTYRAG 5 cut(s) 473, 2196, 2728, 2943, 3192
SmoI CTYRAG 5 cut(s) 473, 2196, 2728, 2943, 3192
SseBI AGGCCT 2 cut(s) 1365, 1675
SsiI CCGC 9 cut(s) 763, 957, 1484, 1649, 2215, 2253, 2519, 2547, 2714
SspI AATATT 1 cut(s) 2088
SspMI CTAG 8 cut(s) 900, 1361, 1376, 2031, 2259, 2277, 2607, 3174
SstI GAGCTC 3 cut(s) 1360, 1409, 2697
StuI AGGCCT 2 cut(s) 1365, 1675
StyI CCWWGG 2 cut(s) 1242, 2606
TaaI ACNGT 7 cut(s) 70, 524, 1657, 1823, 1934, 2429, 2600
TaiI ACGT 5 cut(s) 633, 909, 1023, 2306, 2359
TaqI TCGA 9 cut(s) 542, 548, 838, 1542, 1919, 2197, 2528, 2632, 2868
TaqII GACCGA 1 cut(s) 2388
TatI WGTACW 5 cut(s) 296, 578, 2375, 2509, 2626
TauI GCSGC 1 cut(s) 2716
TfiI GAWTC 7 cut(s) 375, 866, 965, 1009, 1205, 1388, 2885
TscAI CASTG 7 cut(s) 853, 1357, 1726, 1837, 2434, 2723, 3151
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 6 cut(s) 566, 941, 1199, 1924, 2544, 2873
Tsp45I GTSAC 1 cut(s) 644
TspGWI ACGGA 6 cut(s) 47, 381, 674, 2757, 2875, 3001
TspMI CCCGGG 2 cut(s) 379, 426
TspRI CASTG 7 cut(s) 853, 1357, 1726, 1837, 2434, 2723, 3151
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 2549
VspI ATTAAT 2 cut(s) 2007, 2648
XapI RAATTY 4 cut(s) 289, 1319, 1753, 1895
XbaI TCTAGA 1 cut(s) 1375
XcmI CCANNNNNNNNNTGG 1 cut(s) 3149
XhoI CTCGAG 1 cut(s) 2196
XmaI CCCGGG 2 cut(s) 379, 426
XmaJI CCTAGG 1 cut(s) 2606
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 5 cut(s) 360, 670, 1319, 2955, 2995
XspI CTAG 8 cut(s) 900, 1361, 1376, 2031, 2259, 2277, 2607, 3174
ZrmI AGTACT 2 cut(s) 580, 2511
Zsp2I ATGCAT 2 cut(s) 1694, 2758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.