Rh7CG087700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013452
Physical Location & Seq
Forward (+)
6626239 .. 6631277
5039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG087700.1

Sequence Viewer

Length: 3360 bp
ATGAAAGGCCGATCTCACCGGCTTCAGAGTTCGGACCCGCCGGACGACTGGGTGGATGAGTCGTGGACGGTGGATTGTTTGTGTGGTGTGACTTTCGACGATGGGGAAGAGATGGTGAATTGTGATGAGTGTGGTGTTTGGGTGCACACTCGGTGTTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGATAAATGTAAGAGCAGGAATAGTAGGAATGATAGTGAGGAAACTGAGGTTGCGCAATTGTTGGTTGAGCTGCCTACCAAGACTGTGAGAATGGAGAGTTCGTTTCCGCCACCACCGAATATGCCTACTCGGCGCCCTCTTAGGCTTTGGACTGATATACCAATGGAGGAGAGGGTGCATGTGCAGGGAATTCCAGGGGGTGACCCTGCTATATTTGGTGGGTTGTCCTCGGTTTTTACTCCGGAGCTGTGGAAGTCGACGGGATATGTGCCCAAAAAGTTTAATTTTCAGTATAGGGAGTTCCCTTGTTGGGATAAGAAGGAGGAGGCTGATGTTAGATTTGATGAAGACAGTGAGAATGCTGTGGATAAAGGTGCTGGCGTTCTGTTCTCTTTGTTGAATGAGAGTGTCTTGGCAAGCCCAGTGGCTGCTTTGGTTGGAATGAGGAGCCGAGAAGAAGGCGGGTATGAAAAGAAGGTCTCTTTGAAAGAGACAAAAAGGTGGGATAAGGAGGTTCGTGATCTTAGATGTGCTCAGAGTGGTGTGAAGAAGGAGAGGAGTTTACTGCGGCCCATGGTATTACATACGGGTAAGCGTAAGAAGGACGATCTGGGTACATCCAAAGATCGGAGTGCCAAGAAGAGGGCTAGAGCTGCTGAGAAAGAGGCTGATGCAAAGAAGAGAGGGGCTCAATCTTCTAAATCAGTGTTTACACCCTCAAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATTTCTAAGGCTGATGTTCAGAGTATGAAGTACAAGAAGTTGAGCAACTCTGTGGTTAGAGAACCTGCACCAAATGTTTCTCTTGCGACAGACTGTACTGTTGAAAATCACTCTTCAGAAGCCTTACTTTCTGATAGGTCTAAAAAGATTGATGATGGGCTGAAGGAAGACAAGGTGGAGCACCAAGTTTCTGCAGAGCCGGGGCACATGACTATCACCAAAATGGATGGTGCTGCTGTTGCATCATTGTTAGAATTAAATGATGCTAGGACTGATTGTCTACAAGAACAGGGAGATAGCACAGAAGATGAAAATTTGAATGTGAAACCACCCGTTGAAAGTGTTAGCACTGCACCGGAGGTTGAAGTTCAGAATCACTGTCCTACTAGCGACGGGTCCATACAACATTCTCCCAATAAGAAAACTGAAGACCATGAGGACAATTCTCAGAGCCCGGTGAATGTGCAGTCTTCTCTTCATGGTGAGGCGAAAGATCTCGGCAAATCTTCTGATCAAGTGTCTGAAAGTTTGAAACTTAATGGTGTAATAGTAAACATCCCACCGTCCAGTGACCATAAGGTGCAAGGTGCTGACAGAACTTCTGAAGCAGTTAGTGATTCTCATACAGAGAGGGGTGATGAATTATCTGGCGATTGCCAGCCTAAACGGGAATCAGAAGATTTGGCGGATTCAGTTACTCTGCAAAAATGTTCTTCAGATGTCAAACATGGTTTGAAGCTTTCAGAAGATCTTTCAAAACCAGGCGGGATCTTAAACTCTGCAGCAGCATCGGGTCAGCTTAAAACAGCAAGTGCCGGGAAATCTTGTACTGTTCCATCCACCAGTTTGACCCCCAAATCATCCACTCCTCAGAACTTAAAATCTGGTGATGTTCAGAATCCTAATCCATTTACTAAGCAACGAGTAATGTCTGAAAGTAAAGTTAGCATTAAGAAAGATCGTGCTTCATCTGCGGACATGGATCACGATAAGGACAATATGCCAAGGAAAATAGTAAAAGAGCAGTTAAGATCCCCTACAAGTTCTGCTCTGAAAACCTCACACTTCAGCAGGAATTCACATGATTCTGTATCCAAGCGGACCACATCAGAATCAAAAGATTCATTGCTTCACTCATCTTCCAAGACATTGTCAGAAGGGGATACTGCAGTTCCTTCAGGCTCTAGTGAGAAGGTTTTACATGGGCAGAACAAAAGTTCAGCGTCAAGTGCATTGCAAAGAGGTGAAAAGTTAAACCAGACAACTTCTTCTAAGACCAGTCAGAGTCATGCACCACCTTCTTGTCCACCTGCACCATCAAGTTCTCAAGCAAAACTAAGTGATGAGGAACTCGCTTTACTTTTACATCAAGAACTCAACAGTTCTCCCAGAGTACCTCGTGTACCACGTGCTCGTCATGCGTCTAGCTTACCTCAGCTGGCTTCTCCAACTGCAACAAGCATGTTAATAAAGCGTACATCTTCTTCTAGCGGAAAGGATCACAACTCGGGCTCTAGAAGAAAAGTGAGGGATGCATATAAAGATGGGGTCCGAAGTTCCCGTGAACTGGATGATGAAACTAAGAGGATGGACAGAGTCCCATCTTCTCCTGATCCTAGAAGACAAGACGCAGCTAGTACAGTAGATGCTACTAGGAGGGAGGAAAATGCATCTTCAACAGCCTCACATTCTTACAAAAAGACTATCCCTTCTACCTCCATTCCAACTGCAAACAGTGGTCCATCTTCATCCACTGAGGCTAATGATCGCAATGTGTCATCAGTACGCAGTTCACCGAGGAATGCGTCTGACGATGATACAGGGGCAGTTGGTCCTGTTCATCCTACTTTACCAGGCTTGATAAATGAGATTATGAGTAAAGGTAGACGAATGACGTATGAGGAGCTCTGCAATGCTGTGATGCCGCAATGGCATAATCTGAGAAAGCACAATGGAGAGCGTTATGCATATACAAGTCCTTCGCAGGCTGTTCTTGATTGCCTTCGGAATCGACATGAATGGGCTCGATTAGTTGATCGTGGTCCAAAGACAAACCCTAGGAAAAAGCGAAAACTTGATGCTGATGATTCAGAGGATAATGAATATGGCAGGGTCAACAATCCAAAGGAGTTGGAAGGTAAAAGCATAGAGACACAGAGAGAGGACTATCCAAAGGGCAAGAGAAAAGCTCGAAAACGCAGAAGACTGGCCCTTCAAGGAAGAGGAATAAAGGATGTCAGGGAGAAACGGAAGGCAGATTTGCTCACCGATGATGATGTAGGGCCATCATTTTCTAATTCCACAGAGGAAACTGTGTCTGAGGATGATACTCAGGGAGGTGGAGCAGGGGGTCCAGTTGGAAGTGAGGCCTCTTCTAGTTCAGAGGAGGAAGGAACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1119

Amino Acids

122.48

Weight (kDa)

7.0

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 935 - 997 8.5e-33 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2275
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 2 cut(s) 1030, 2275
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 3 cut(s) 449, 1237, 2842
AccIII TCCGGA 1 cut(s) 433
AclWI GGATC 5 cut(s) 1733, 1947, 1983, 2463, 2564
AcsI RAATTY 4 cut(s) 381, 932, 1270, 2032
AcuI CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2118
AcvI CACGTG 1 cut(s) 2366
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 9 cut(s) 808, 989, 1054, 1786, 2352, 2361, 2434, 2596, 2742
AfiI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
AhdI GACNNNNNGTC 1 cut(s) 1233
AjnI CCWGG 3 cut(s) 385, 1717, 2809
Alw21I GWGCWC 5 cut(s) 147, 727, 1140, 2371, 2865
Alw26I GTCTC 3 cut(s) 676, 677, 3101
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 5 cut(s) 1733, 1947, 1983, 2463, 2564
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 2464
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 5 cut(s) 7, 761, 3165, 3239, 3324
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 7 cut(s) 262, 620, 845, 1190, 1739, 1742, 2588
ApoI RAATTY 4 cut(s) 381, 932, 1270, 2032
AspA2I CCTAGG 1 cut(s) 3014
AspLEI GCGC 2 cut(s) 247, 327
AsuC2I CCSGG 3 cut(s) 1158, 1412, 1774
AvaI CYCGRG 1 cut(s) 2464
AvaII GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3308
AvrII CCTAGG 1 cut(s) 3014
BaeGI GKGCMC 3 cut(s) 147, 465, 1164
BaeI ACNNNNGTAYC 2 cut(s) 2112, 2145
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
BarI GAAGNNNNNNTAC 2 cut(s) 2425, 2457
BauI CACGAG 1 cut(s) 2355
BbrPI CACGTG 1 cut(s) 2366
BbsI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2584, 3166
Bbv12I GWGCWC 5 cut(s) 147, 727, 1140, 2371, 2865
BbvCI CCTCAGC 1 cut(s) 2391
BbvI GCAGC 7 cut(s) 249, 607, 832, 1177, 1751, 1754, 2600
BcgI CGANNNNNNTGC 2 cut(s) 1728, 1762
BciT130I CCWGG 3 cut(s) 387, 1719, 2811
BciVI GTATCC 2 cut(s) 2059, 2113
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 3 cut(s) 1158, 1412, 1774
BcoDI GTCTC 3 cut(s) 676, 677, 3101
BfmI CTRYAG 3 cut(s) 1149, 1737, 2124
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 2 cut(s) 1030, 2275
BfuI GTATCC 2 cut(s) 2059, 2113
BglI GCCNNNNNGGC 2 cut(s) 322, 2887
BglII AGATCT 2 cut(s) 1450, 1705
BisI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1740, 1743, 2589, 2882
BlnI CCTAGG 1 cut(s) 3014
BlsI GCNGC 9 cut(s) 264, 622, 762, 847, 1192, 1741, 1744, 2590, 2883
Bme1390I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2811
Bme18I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3308
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 1 cut(s) 2464
BmiI GGNNCC 6 cut(s) 36, 326, 641, 1354, 2507, 3309
BmrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2811
BmrI ACTGGG 2 cut(s) 58, 608
BmuI ACTGGG 2 cut(s) 58, 608
BoxI GACNNNNGTC 1 cut(s) 948
BpiI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2584, 3166
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 3130, 3162
Bpu10I CCTNAGC 1 cut(s) 2391
BpuEI CTTGAG 2 cut(s) 895, 2268
BpuMI CCSGG 3 cut(s) 1158, 1412, 1774
BsaAI YACGTR 1 cut(s) 2366
BsaHI GRCGYC 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2753, 3014
BsaWI WCCGGW 2 cut(s) 433, 1312
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 8 cut(s) 53, 614, 1524, 1800, 2235, 2529, 3168, 3311
Bse3DI GCAATG 5 cut(s) 2081, 2189, 2734, 2875, 2891
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 3 cut(s) 387, 1719, 2811
BseDI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2753, 3014
BseLI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
BseMI GCAATG 5 cut(s) 2081, 2189, 2734, 2875, 2891
BseNI ACTGG 8 cut(s) 53, 614, 1524, 1800, 2235, 2529, 3168, 3311
BseRI GAGGAG 7 cut(s) 374, 530, 652, 763, 1815, 2873, 3356
BseSI GKGCMC 3 cut(s) 147, 465, 1164
BseXI GCAGC 7 cut(s) 249, 607, 832, 1177, 1751, 1754, 2600
BsgI GTGCAG 5 cut(s) 395, 1008, 1293, 1442, 2253
BshFI GGCC 5 cut(s) 9, 763, 3167, 3241, 3326
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 5 cut(s) 147, 727, 1140, 2371, 2865
BsiHKCI CYCGRG 1 cut(s) 2464
BsiSI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
BslFI GGGAC 1 cut(s) 2540
BslI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
BsmAI GTCTC 3 cut(s) 676, 677, 3101
BsmFI GGGAC 1 cut(s) 2540
BsmI GAATGC 2 cut(s) 556, 2764
BsnI GGCC 5 cut(s) 9, 763, 3167, 3241, 3326
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 2464
Bsp13I TCCGGA 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 765
BspANI GGCC 5 cut(s) 9, 763, 3167, 3241, 3326
BspEI TCCGGA 1 cut(s) 433
BspLI GGNNCC 6 cut(s) 36, 326, 641, 1354, 2507, 3309
BspMAI CTGCAG 3 cut(s) 1153, 1741, 2128
BspMI ACCTGC 2 cut(s) 1030, 2275
BspPI GGATC 5 cut(s) 1733, 1947, 1983, 2463, 2564
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 676
BsrDI GCAATG 5 cut(s) 2081, 2189, 2734, 2875, 2891
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 8 cut(s) 53, 614, 1524, 1800, 2235, 2529, 3168, 3311
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2753, 3014
BssNI GRCGYC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 2355
BssT1I CCWWGG 3 cut(s) 765, 1961, 3014
Bst2BI CACGAG 1 cut(s) 2355
Bst2UI CCWGG 3 cut(s) 387, 1719, 2811
Bst6I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3334
BstACI GRCGYC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 2366
BstC8I GCNNGC 5 cut(s) 571, 610, 1616, 2397, 2943
BstDSI CCRYGG 1 cut(s) 765
BstEII GGTNACC 1 cut(s) 392
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 2 cut(s) 247, 327
BstMAI GTCTC 3 cut(s) 676, 677, 3101
BstMWI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
BstNI CCWGG 3 cut(s) 387, 1719, 2811
BstNSI RCATGY 2 cut(s) 374, 2422
BstPAI GACNNNNGTC 1 cut(s) 948
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1772, 2809
BstSFI CTRYAG 3 cut(s) 1149, 1737, 2124
BstSLI GKGCMC 3 cut(s) 147, 465, 1164
BstV1I GCAGC 7 cut(s) 249, 607, 832, 1177, 1751, 1754, 2600
BstV2I GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2584, 3166
BstX2I RGATCY 4 cut(s) 1450, 1705, 1725, 1988
BstYI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
BsuI GTATCC 2 cut(s) 2059, 2113
BsuRI GGCC 5 cut(s) 9, 763, 3167, 3241, 3326
BtgI CCRYGG 1 cut(s) 765
BtsI GCAGTG 1 cut(s) 1305
BtsIMutI CAGTG 8 cut(s) 550, 621, 903, 1305, 1333, 1531, 2698, 2709
BveI ACCTGC 2 cut(s) 1030, 2275
Cac8I GCNNGC 5 cut(s) 571, 610, 1616, 2397, 2943
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 2 cut(s) 247, 327
Cfr10I RCCGGY 1 cut(s) 18
CseI GACGC 4 cut(s) 2169, 2367, 2594, 2751
Csp6I GTAC 9 cut(s) 807, 988, 1053, 1785, 2351, 2360, 2433, 2595, 2741
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
CviQI GTAC 9 cut(s) 807, 988, 1053, 1785, 2351, 2360, 2433, 2595, 2741
DinI GGCGCC 1 cut(s) 326
DraIII CACNNNGTG 1 cut(s) 153
DriI GACNNNNNGTC 1 cut(s) 1233
Eam1104I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3334
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3334
EciI GGCGGA 2 cut(s) 288, 1658
Ecl136II GAGCTC 1 cut(s) 2863
Eco130I CCWWGG 3 cut(s) 765, 1961, 3014
Eco147I AGGCCT 1 cut(s) 3326
Eco24I GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
Eco31I GGTCTC 1 cut(s) 676
Eco47I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3308
Eco53kI GAGCTC 1 cut(s) 2863
Eco57I CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2118
Eco72I CACGTG 1 cut(s) 2366
Eco88I CYCGRG 1 cut(s) 2464
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 2863
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 381, 2032
EcoRII CCWGG 3 cut(s) 385, 1717, 2809
EcoT14I CCWWGG 3 cut(s) 765, 1961, 3014
EcoT22I ATGCAT 3 cut(s) 2494, 2629, 2926
EcoT38I GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 3 cut(s) 765, 1961, 3014
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 1 cut(s) 2540
FauI CCCGC 3 cut(s) 45, 647, 1715
FbaI TGATCA 1 cut(s) 1468
FblI GTMKAC 3 cut(s) 449, 1237, 2842
Fnu4HI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1740, 1743, 2589, 2882
FriOI GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
Fsp4HI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1740, 1743, 2589, 2882
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 2 cut(s) 246, 326
GluI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1740, 1743, 2589, 2882
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 5 cut(s) 9, 763, 3167, 3241, 3326
HapII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
HgaI GACGC 4 cut(s) 2169, 2367, 2594, 2751
HhaI GCGC 2 cut(s) 247, 327
Hin1I GRCGYC 1 cut(s) 325
Hin6I GCGC 2 cut(s) 245, 325
HinP1I GCGC 2 cut(s) 245, 325
HincII GTYRAC 2 cut(s) 450, 3073
HindII GTYRAC 2 cut(s) 450, 3073
HindIII AAGCTT 1 cut(s) 1694
HpaII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
Hpy188III TCNNGA 7 cut(s) 434, 710, 1943, 2327, 2472, 2567, 2951
Hpy99I CGWCG 3 cut(s) 101, 454, 1352
HpyCH4IV ACGT 2 cut(s) 2365, 2852
HpyF10VI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
HpySE526I ACGT 2 cut(s) 2365, 2852
Hsp92I GRCGYC 1 cut(s) 325
HspAI GCGC 2 cut(s) 245, 325
KasI GGCGCC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 1468
LmnI GCTCC 5 cut(s) 436, 639, 1135, 2860, 3299
Lsp1109I GCAGC 7 cut(s) 249, 607, 832, 1177, 1751, 1754, 2600
MaeII ACGT 2 cut(s) 2365, 2852
MaeIII GTNAC 4 cut(s) 88, 392, 1526, 1651
MfeI CAATTG 2 cut(s) 248, 926
MflI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 3 cut(s) 68, 2251, 2562
MmeI TCCRAC 5 cut(s) 610, 2429, 2705, 3069, 3295
Mph1103I ATGCAT 3 cut(s) 2494, 2629, 2926
MroI TCCGGA 1 cut(s) 433
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 2395
MspI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
MspR9I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2811
MunI CAATTG 2 cut(s) 248, 926
Mva1269I GAATGC 2 cut(s) 556, 2764
MvaI CCWGG 3 cut(s) 387, 1719, 2811
MwoI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
NarI GGCGCC 1 cut(s) 325
NciI CCSGG 3 cut(s) 1158, 1412, 1774
NcoI CCATGG 1 cut(s) 765
NlaIV GGNNCC 6 cut(s) 36, 326, 641, 1354, 2507, 3309
NmeAIII GCCGAG 3 cut(s) 301, 668, 1434
NmuCI GTSAC 3 cut(s) 88, 392, 1526
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 3 cut(s) 2494, 2629, 2926
NspI RCATGY 2 cut(s) 374, 2422
PaqCI CACCTGC 1 cut(s) 2275
PceI AGGCCT 1 cut(s) 3326
PcsI WCGNNNNNNNCGW 1 cut(s) 2362
PctI GAATGC 2 cut(s) 556, 2764
PflFI GACNNNGTC 2 cut(s) 2107, 2552
PkrI GCNGC 9 cut(s) 264, 622, 762, 847, 1192, 1741, 1744, 2590, 2883
PleI GAGTC 3 cut(s) 67, 2250, 2561
PluTI GGCGCC 1 cut(s) 328
PmaCI CACGTG 1 cut(s) 2366
PmlI CACGTG 1 cut(s) 2366
PpsI GAGTC 3 cut(s) 67, 2250, 2561
Ppu21I YACGTR 1 cut(s) 2366
PshAI GACNNNNGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 2865
Psp6I CCWGG 3 cut(s) 385, 1717, 2809
PspCI CACGTG 1 cut(s) 2366
PspEI GGTNACC 1 cut(s) 392
PspGI CCWGG 3 cut(s) 385, 1717, 2809
PspN4I GGNNCC 6 cut(s) 36, 326, 641, 1354, 2507, 3309
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2298, 2330
PstI CTGCAG 3 cut(s) 1153, 1741, 2128
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
PsyI GACNNNGTC 2 cut(s) 2107, 2552
PvuII CAGCTG 1 cut(s) 2395
RsaI GTAC 9 cut(s) 808, 989, 1054, 1786, 2352, 2361, 2434, 2596, 2742
RsaNI GTAC 9 cut(s) 807, 988, 1053, 1785, 2351, 2360, 2433, 2595, 2741
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 2865
SalI GTCGAC 1 cut(s) 448
SatI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1740, 1743, 2589, 2882
SchI GAGTC 3 cut(s) 68, 2251, 2562
ScrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2811
SfcI CTRYAG 3 cut(s) 1149, 1737, 2124
SfoI GGCGCC 1 cut(s) 326
SinI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3308
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 910, 2283
SmoI CTYRAG 2 cut(s) 910, 2283
SseBI AGGCCT 1 cut(s) 3326
SspDI GGCGCC 1 cut(s) 324
SstI GAGCTC 1 cut(s) 2865
StuI AGGCCT 1 cut(s) 3326
StyD4I CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1772, 2809
StyI CCWWGG 3 cut(s) 765, 1961, 3014
TaiI ACGT 2 cut(s) 2368, 2855
TaqI TCGA 5 cut(s) 96, 449, 2968, 2983, 3148
TatI WGTACW 4 cut(s) 987, 1052, 1784, 2594
TauI GCSGC 2 cut(s) 763, 2884
TscAI CASTG 8 cut(s) 550, 621, 903, 1312, 1340, 1531, 2698, 2716
TseFI GTSAC 3 cut(s) 88, 392, 1526
TseI GCWGC 7 cut(s) 262, 620, 845, 1190, 1739, 1742, 2588
Tsp45I GTSAC 3 cut(s) 88, 392, 1526
TspGWI ACGGA 1 cut(s) 3220
TspRI CASTG 8 cut(s) 550, 621, 903, 1312, 1340, 1531, 2698, 2716
Tth111I GACNNNGTC 2 cut(s) 2107, 2552
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3308
XapI RAATTY 4 cut(s) 381, 932, 1270, 2032
XbaI TCTAGA 1 cut(s) 2471
XceI RCATGY 2 cut(s) 374, 2422
XmaJI CCTAGG 1 cut(s) 3014
XmiI GTMKAC 3 cut(s) 449, 1237, 2842
Zsp2I ATGCAT 3 cut(s) 2494, 2629, 2926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.