MD06G1121800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013452
Physical Location & Seq
Forward (+)
26313668 .. 26319322
5655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1121800.v1.1.491

Sequence Viewer

Length: 3390 bp
ATGAAGGGTCGGTCTCACCGGCTTCAGAGTTCGGACCCGCCGGACGACTGGGTTAACGGGTCGTGGACCGTGGACTGCCTGTGCGGTGTCAATTTTGACGATGGGGAAGAGATGGTCAATTGCGACGAGTGTAGCGTTTGGGTGCACACCCGGTGCTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGACAAGTGTAAGAGTAAGAACAATAGGAATGATAGTGAGGAAACCGAGGTTGCGCAGTTGTTGGTTGAGCTTCCGACGAAGACTGTGAGGATGGAGAGCTCTTATGCACATCCACCTAATGTGCCTACCAGGCGGCCGTTCCGGCTTTGGACTGATATACCGATGGAGGAGAGGGTGCATGTTCAGGGAATCCCGGGAGGGGACCCTGCTCTATTTGGTGGGTTGTCGTCTGTTTTTACTCCGGAGCTGTGGAAGTCTACTGGGTATGTCCCCAAAAAGTTTAATTTTCAGTATAGGGAGTTCCCTTGTTGGGATGAGAAGAAGGAGGATGATGCTAAATTTGACGAGGAAAATGAGAATCCGGTTGATAGAGGTGCTGGTGTTCTGTTCTCCTTGTTGAAGGAGAGTGTGTTGGCAAACCCAGTAGCTTCTTTGGTGGGGATGAGGGGCCGAACAGAGGATGGGGGCTATAACAAGAATGCATCTTTGAAAGAGAGGAAAAGGTGGGATAATGAAGCTGTTGATCTTAGGTGTGCTCAGAGTGGAGTGAAGAAGGAGAGAAGTCTACTTCGGCCTGTTGTGTTACATTCGGGCAAGCGGAAAAAGGATGATTTGGGGACATCCAAAGATCGGAGTGGCAAGAAGAAGGCTAGAGCTGCTGAGAAAGAGGCTGATGCAAAGAAGAGAGGTGCACAATCTTCTAAATCAGTGTTTACACCCACGAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATTTCCAAGGGTGACATTCAGAGTAAGAAGAGCAAGAAGTTTAGCGACTCCGTGGTCAGAGAACCTGCATCAGATGGTTGTCTTCCAGCAGACTCTACTGTTGAAAAACACTCATCAGAATCCTTGATTTCTGACACGCGTAAACAGAAAATTTCAATTGGTGATGGACTGAAGGAAGACAAGGTTGGCCACCAAGTTCCTGCAGTGCCGGAGAATCTCACTCTCACCAAAACTGTTGATGCTGTTGCTTCATTGTTAGAGCACAATGATGGTGCAGCTGATTGTGAAAAGAAAGAGGGAGGTAGGACAGCAGATGATACGTTGGATGTGCAACCTCTAATTGGAGATGTTGCTGCTCCAGAAGTTAAGAATCAGATTCAATATTCTAATGGTGGTATCTCTGTGGAACCTCATTCTAAATTGAAAACAGAAGATCATGATGAGAATTGTAGGAGCTCATTGAACATTCAGCATTCTCCTCATGCTGATGCAAAAGATCTCAGTGTATCTTCTGATCACAGGTCTGAAAGTCTGAGAATCAATGAGGTACTAGTAAACAGCCCACTATCTAGTGACCATAAGGTGCTTGGTGCTGACAGAAATTCAGAAGTGGCTAGTGATTCTTGTAAAGACAAGAGCGATGAGTTATCTGGTGATCCTTGTCAACTTAAACAGGAATTGGAAGGTTCTGAGGGTTCCATGGCTCTGCAACAAAGTCCTTCAGATCCCAAACATAGTTTGGGGTCTCCTGAAGAGCTTTCAAAACCAGATGGAATTATCTTCAACTCTCCAGCAATACCAAGTCAGTGTAAAACAGTAGCATGTGCTGGAAAATCTTCTGCTGTTTCATTAACCGTTGCAGTCTCCATATCATCCGCTTCTGATATCTTAAAATCTGGTGATGCTCAGAATCCTCATCCAATTTTGAAGCAACGAGTAATATCTGAAAGTAATGTTAGCACTAAGAAGGATCGTGCTTCATGTGACGACTTGGATGAAGACAGGGACAATATGTCAAGGAAAACAGTAAAAGAGCATGTCAGATCCTCTACAAATTCTACTCTGAAAACCTCACACTCGAGCAGGAATCATGATTCTAAGTGGGCCACATCAGAATCAAAAGATTCAGTGCATCACTCATCTTCCAAGACGTCTCCAGCAGGGAATACCACTGTTCCTTCAGGATCTAGTGAGCCTGCTGGATCACTGCCCAGCCAGAAGGTTTTACATGTACAAAATAAGAGTTCAGCTTCTAGTGCATTGCAAAGAGGTGAAAAGTTCAACCATACAACTTCATCTAAGACTAATCAGAATCACACACCATCTGCTTGTCCCCCTGCACCACCAAGTGTGCAGGCACAATTAACTGACCAGGAGATAGCTATGCTTTTGCATCAAGAGCTCAACAGTTCTCCAAGAGTACCTCGTGTACCACGTGTTCGCAATGCATCTAGCTTACCTCAATTAACTTCAAGTGCAACAAGCACGTTAATGAAGCGTACATCTAATTCTGGAGGAAAGGATCACAGTTCGGTCTTTAGAAGAAAAATCAGGGATGCACCTAAAGATGGGTCCCGAAGTTCACGTGAACATGATGAAGAAGCTAAAAGGACGGGGAGAATACCATCTTCTCCGGATCGAAGACGACAAGATGCAGCAGATATTGCTAGTAAGAGGGAGGACAATGGATCTTCAGCAGCGGTACTCTCTGGCAGGAAGAATATTCATTCTTCCTCCACTCATACTGCAAACAGTGGACCATCTTCGTCCAATGAGGCTAATGATCGCAATGTGTCATCTGTACGCAGTTCGCCTATGAATGTCTCTGATGATGATACAGGCTCAGTTGGTCCGATTCATCGTACATTACCAGCCTTGATAAATGAGATTATGAGTAAAGGCAGACGCATGACATATGAGGAGCTTTGTAATGCTGTGATGCCGCACTGGCTTAACCTGAGAAAGCATAATGGAGAGCGTTATGCATACACAAGTCCTTCACAGGCTGTTCTTGATTGCCTTCGAAATCGACATGAATGGGCTCGGTTAGTTGATCGTGGTCCTAAGACAAATTCAAGTAGGAAAAGGCGAAAAGCAGATGCTGAAGATTCAGATGATAATGAATATGGCAGGGGAAAAAATCCAAAGGAGTTGGATGGTAAAAGCATTGAGACACAGAGAGAGGACTACCCCAAGGGCAAGAGAAAAGCTCGAAAACGTAGGAGATTGGCTCTTCAGGGAAAAGGAATAAAGGATGTCAGGGAGAAGCGGAAGGCAGATATGCTTACCGACGATGATGTTGGACAGTCATTTTCTAATTCCACTGAAGGAAGCATGTCCACTGAGGATGATATTCAGGGAGGCGGAGCATGCCCGGTAAGAGGAAGTGAGGCCTCTACTAGTTCGGATGATGAGACCGGGGCAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1130

Amino Acids

123.43

Weight (kDa)

7.93

Isoelectric Point (pI)

54.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 942 - 1004 2.6e-32 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1013
AatII GACGTC 1 cut(s) 2114
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 1033
AccI GTMKAC 2 cut(s) 449, 757
AccII CGCG 1 cut(s) 1099
AccIII TCCGGA 2 cut(s) 433, 2593
AciI CCGC 9 cut(s) 38, 84, 325, 790, 1836, 2660, 2903, 3229, 3324
AclWI GGATC 9 cut(s) 1610, 1679, 1938, 1998, 2152, 2170, 2490, 2604, 2656
AcoI YGGCCR 2 cut(s) 326, 1147
AcsI RAATTY 6 cut(s) 530, 935, 1110, 1561, 2014, 3031
AcuI CTGAAG 9 cut(s) 8, 1151, 1665, 1731, 2124, 2637, 3084, 3179, 3306
AcvI CACGTG 2 cut(s) 2396, 2546
AcyI GRCGYC 1 cut(s) 2111
AdeI CACNNNGTG 2 cut(s) 153, 2309
AfaI GTAC 8 cut(s) 1509, 2193, 2382, 2391, 2461, 2664, 2763, 2824
AfiI CCNNNNNNNGG 7 cut(s) 391, 502, 649, 822, 1301, 2528, 3341
AflIII ACRYGT 3 cut(s) 1097, 2188, 2395
AhdI GACNNNNNGTC 1 cut(s) 1972
AhlI ACTAGT 2 cut(s) 1510, 3359
AjnI CCWGG 2 cut(s) 320, 2331
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
Alw21I GWGCWC 8 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364
Alw26I GTCTC 7 cut(s) 18, 1710, 1828, 2118, 2788, 3125, 3368
Alw44I GTGCAC 2 cut(s) 143, 882
AlwI GGATC 9 cut(s) 1610, 1679, 1938, 1998, 2152, 2170, 2490, 2604, 2656
AlwNI CAGNNNCTG 1 cut(s) 3062
Ama87I CYCGRG 2 cut(s) 385, 2038
Aor13HI TCCGGA 2 cut(s) 433, 2593
AoxI GGCC 6 cut(s) 326, 640, 764, 1147, 2064, 3351
ApaLI GTGCAC 2 cut(s) 143, 882
ApeKI GCWGC 5 cut(s) 848, 1235, 1313, 2615, 2657
ApoI RAATTY 6 cut(s) 530, 935, 1110, 1561, 2014, 3031
Asp700I GAANNNNTTC 1 cut(s) 1795
AspLEI GCGC 1 cut(s) 247
AsuC2I CCSGG 5 cut(s) 151, 386, 387, 3335, 3379
AsuHPI GGTGA 7 cut(s) 8, 983, 1133, 1177, 1625, 1871, 2243
AsuII TTCGAA 1 cut(s) 2983
AvaI CYCGRG 2 cut(s) 385, 2038
AvaII GGWCC 8 cut(s) 34, 66, 394, 953, 2532, 2717, 2810, 3020
BaeGI GKGCMC 2 cut(s) 147, 886
BalI TGGCCA 1 cut(s) 1149
BanII GRGCYC 4 cut(s) 293, 1418, 2364, 3004
BauI CACGAG 2 cut(s) 913, 2385
BbrPI CACGTG 2 cut(s) 2396, 2546
BbsI GAAGAC 5 cut(s) 278, 1034, 1143, 1965, 2608
Bbv12I GWGCWC 8 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364
BbvI GCAGC 5 cut(s) 835, 1247, 1300, 2627, 2669
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 2 cut(s) 322, 2333
BclI TGATCA 1 cut(s) 1474
BcnI CCSGG 5 cut(s) 151, 386, 387, 3335, 3379
BcoDI GTCTC 7 cut(s) 18, 1710, 1828, 2118, 2788, 3125, 3368
BcuI ACTAGT 2 cut(s) 1510, 3359
BfmI CTRYAG 1 cut(s) 1161
BfuAI ACCTGC 1 cut(s) 1033
BglI GCCNNNNNGGC 3 cut(s) 322, 334, 2908
BglII AGATCT 1 cut(s) 1456
BisI GCNGC 7 cut(s) 326, 849, 1236, 1314, 2616, 2658, 2903
BlsI GCNGC 7 cut(s) 327, 850, 1237, 1315, 2617, 2659, 2904
Bme1390I CCNGG 7 cut(s) 151, 322, 386, 387, 2333, 3335, 3379
Bme18I GGWCC 8 cut(s) 34, 66, 394, 953, 2532, 2717, 2810, 3020
BmeRI GACNNNNNGTC 1 cut(s) 1972
BmeT110I CYCGRG 2 cut(s) 385, 2038
BmiI GGNNCC 8 cut(s) 36, 395, 396, 641, 1368, 1657, 2533, 2534
BmrFI CCNGG 7 cut(s) 151, 322, 386, 387, 2333, 3335, 3379
BmrI ACTGGG 3 cut(s) 58, 462, 608
BmuI ACTGGG 3 cut(s) 58, 462, 608
BoxI GACNNNNGTC 1 cut(s) 951
BpiI GAAGAC 5 cut(s) 278, 1034, 1143, 1965, 2608
BplI GAGNNNNNCTC 4 cut(s) 3154, 3186, 3175, 3207
BpmI CTGGAG 4 cut(s) 1302, 1734, 2100, 2493
Bpu14I TTCGAA 1 cut(s) 2983
BpuMI CCSGG 5 cut(s) 151, 386, 387, 3335, 3379
BsaAI YACGTR 2 cut(s) 2396, 2546
BsaHI GRCGYC 1 cut(s) 2111
BsaI GGTCTC 3 cut(s) 18, 1710, 3368
BsaJI CCNNGG 8 cut(s) 69, 237, 385, 966, 1011, 1659, 3153, 3378
BsaWI WCCGGW 3 cut(s) 433, 553, 2593
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 7 cut(s) 391, 502, 649, 822, 1301, 2528, 3341
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 4 cut(s) 53, 457, 614, 2912
Bse3DI GCAATG 3 cut(s) 2219, 2410, 2755
BseAI TCCGGA 2 cut(s) 433, 2593
BseBI CCWGG 2 cut(s) 322, 2333
BseDI CCNNGG 8 cut(s) 69, 237, 385, 966, 1011, 1659, 3153, 3378
BseLI CCNNNNNNNGG 7 cut(s) 391, 502, 649, 822, 1301, 2528, 3341
BseMI GCAATG 3 cut(s) 2219, 2410, 2755
BseNI ACTGG 4 cut(s) 53, 457, 614, 2912
BseRI GAGGAG 3 cut(s) 374, 1428, 2894
BseSI GKGCMC 2 cut(s) 147, 886
BseX3I CGGCCG 1 cut(s) 326
BseXI GCAGC 5 cut(s) 835, 1247, 1300, 2627, 2669
BseYI CCCAGC 1 cut(s) 2171
BsgI GTGCAG 3 cut(s) 1254, 2283, 2333
Bsh1236I CGCG 1 cut(s) 1099
Bsh1285I CGRYCG 1 cut(s) 329
BshFI GGCC 6 cut(s) 328, 642, 766, 1149, 2066, 3353
BsiEI CGRYCG 1 cut(s) 329
BsiHKAI GWGCWC 8 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364
BsiHKCI CYCGRG 2 cut(s) 385, 2038
BslFI GGGAC 6 cut(s) 407, 446, 823, 1979, 2277, 2518
BslI CCNNNNNNNGG 7 cut(s) 391, 502, 649, 822, 1301, 2528, 3341
BsmAI GTCTC 7 cut(s) 18, 1710, 1828, 2118, 2788, 3125, 3368
BsmBI CGTCTC 1 cut(s) 2118
BsmFI GGGAC 6 cut(s) 407, 446, 823, 1979, 2277, 2518
BsmI GAATGC 2 cut(s) 676, 1432
BsnI GGCC 6 cut(s) 328, 642, 766, 1149, 2066, 3353
Bso31I GGTCTC 3 cut(s) 18, 1710, 3368
BsoBI CYCGRG 2 cut(s) 385, 2038
Bsp119I TTCGAA 1 cut(s) 2983
Bsp1286I GDGCHC 9 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364, 3004
Bsp13I TCCGGA 2 cut(s) 433, 2593
Bsp1407I TGTACA 1 cut(s) 2191
Bsp19I CCATGG 1 cut(s) 1659
BspACI CCGC 9 cut(s) 38, 84, 325, 790, 1836, 2660, 2903, 3229, 3324
BspANI GGCC 6 cut(s) 328, 642, 766, 1149, 2066, 3353
BspEI TCCGGA 2 cut(s) 433, 2593
BspFNI CGCG 1 cut(s) 1099
BspHI TCATGA 2 cut(s) 1396, 2050
BspLI GGNNCC 8 cut(s) 36, 395, 396, 641, 1368, 1657, 2533, 2534
BspMAI CTGCAG 1 cut(s) 1165
BspMI ACCTGC 1 cut(s) 1033
BspPI GGATC 9 cut(s) 1610, 1679, 1938, 1998, 2152, 2170, 2490, 2604, 2656
BspQI GCTCTTC 3 cut(s) 983, 1707, 3198
BspT104I TTCGAA 1 cut(s) 2983
BspTNI GGTCTC 3 cut(s) 18, 1710, 3368
BsrDI GCAATG 3 cut(s) 2219, 2410, 2755
BsrFI RCCGGY 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 2191
BsrI ACTGG 4 cut(s) 53, 457, 614, 2912
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 8 cut(s) 69, 237, 385, 966, 1011, 1659, 3153, 3378
BssNI GRCGYC 1 cut(s) 2111
BssSI CACGAG 2 cut(s) 913, 2385
BssT1I CCWWGG 3 cut(s) 966, 1659, 3153
Bst2BI CACGAG 2 cut(s) 913, 2385
Bst2UI CCWGG 2 cut(s) 322, 2333
Bst6I CTCTTC 5 cut(s) 102, 869, 983, 1707, 3198
BstACI GRCGYC 1 cut(s) 2111
BstAPI GCANNNNNTGC 1 cut(s) 2624
BstAUI TGTACA 1 cut(s) 2191
BstBAI YACGTR 2 cut(s) 2396, 2546
BstBI TTCGAA 1 cut(s) 2983
BstC8I GCNNGC 4 cut(s) 788, 2157, 2316, 3331
BstDSI CCRYGG 3 cut(s) 69, 1011, 1659
BstFNI CGCG 1 cut(s) 1099
BstHHI GCGC 1 cut(s) 247
BstMAI GTCTC 7 cut(s) 18, 1710, 1828, 2118, 2788, 3125, 3368
BstMCI CGRYCG 1 cut(s) 329
BstMWI GCNNNNNNNGC 8 cut(s) 322, 334, 848, 2216, 2359, 2624, 2908, 3330
BstNI CCWGG 2 cut(s) 322, 2333
BstNSI RCATGY 6 cut(s) 374, 1785, 2000, 2192, 3298, 3333
BstPAI GACNNNNGTC 1 cut(s) 951
BstSCI CCNGG 7 cut(s) 149, 320, 384, 385, 2331, 3333, 3377
BstSFI CTRYAG 1 cut(s) 1161
BstSLI GKGCMC 2 cut(s) 147, 886
BstUI CGCG 1 cut(s) 1099
BstV1I GCAGC 5 cut(s) 835, 1247, 1300, 2627, 2669
BstV2I GAAGAC 5 cut(s) 278, 1034, 1143, 1965, 2608
BstX2I RGATCY 5 cut(s) 1456, 1684, 2003, 2144, 2648
BstYI RGATCY 5 cut(s) 1456, 1684, 2003, 2144, 2648
BstZI CGGCCG 1 cut(s) 326
BsuRI GGCC 6 cut(s) 328, 642, 766, 1149, 2066, 3353
BtgI CCRYGG 3 cut(s) 69, 1011, 1659
BtgZI GCGATG 1 cut(s) 1614
BtsI GCAGTG 2 cut(s) 1170, 2165
BveI ACCTGC 1 cut(s) 1033
Cac8I GCNNGC 4 cut(s) 788, 2157, 2316, 3331
CaiI CAGNNNCTG 1 cut(s) 3062
CciI TCATGA 2 cut(s) 1396, 2050
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 18
Cfr9I CCCGGG 1 cut(s) 385
CseI GACGC 1 cut(s) 2874
Csp6I GTAC 8 cut(s) 1508, 2192, 2381, 2390, 2460, 2663, 2762, 2823
CviQI GTAC 8 cut(s) 1508, 2192, 2381, 2390, 2460, 2663, 2762, 2823
DraIII CACNNNGTG 2 cut(s) 153, 2309
DrdI GACNNNNNNGTC 1 cut(s) 1013
DriI GACNNNNNGTC 1 cut(s) 1972
DseDI GACNNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 2 cut(s) 326, 1147
EagI CGGCCG 1 cut(s) 326
Eam1104I CTCTTC 5 cut(s) 102, 869, 983, 1707, 3198
Eam1105I GACNNNNNGTC 1 cut(s) 1972
EarI CTCTTC 5 cut(s) 102, 869, 983, 1707, 3198
EciI GGCGGA 1 cut(s) 3339
Ecl136II GAGCTC 3 cut(s) 291, 1416, 2362
EclXI CGGCCG 1 cut(s) 326
Eco130I CCWWGG 3 cut(s) 966, 1659, 3153
Eco147I AGGCCT 1 cut(s) 3353
Eco24I GRGCYC 4 cut(s) 293, 1418, 2364, 3004
Eco31I GGTCTC 3 cut(s) 18, 1710, 3368
Eco32I GATATC 1 cut(s) 1846
Eco47I GGWCC 8 cut(s) 34, 66, 394, 953, 2532, 2717, 2810, 3020
Eco52I CGGCCG 1 cut(s) 326
Eco53kI GAGCTC 3 cut(s) 291, 1416, 2362
Eco57I CTGAAG 9 cut(s) 8, 1151, 1665, 1731, 2124, 2637, 3084, 3179, 3306
Eco72I CACGTG 2 cut(s) 2396, 2546
Eco88I CYCGRG 2 cut(s) 385, 2038
EcoICRI GAGCTC 3 cut(s) 291, 1416, 2362
EcoO109I RGGNCCY 2 cut(s) 394, 2532
EcoRII CCWGG 2 cut(s) 320, 2331
EcoRV GATATC 1 cut(s) 1846
EcoT14I CCWWGG 3 cut(s) 966, 1659, 3153
EcoT22I ATGCAT 3 cut(s) 676, 2410, 2947
EcoT38I GRGCYC 4 cut(s) 293, 1418, 2364, 3004
ErhI CCWWGG 3 cut(s) 966, 1659, 3153
Esp3I CGTCTC 1 cut(s) 2118
FaqI GGGAC 6 cut(s) 407, 446, 823, 1979, 2277, 2518
FauI CCCGC 1 cut(s) 45
FauNDI CATATG 1 cut(s) 2875
FbaI TGATCA 1 cut(s) 1474
FblI GTMKAC 2 cut(s) 449, 757
Fnu4HI GCNGC 7 cut(s) 326, 849, 1236, 1314, 2616, 2658, 2903
FriOI GRGCYC 4 cut(s) 293, 1418, 2364, 3004
Fsp4HI GCNGC 7 cut(s) 326, 849, 1236, 1314, 2616, 2658, 2903
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 1 cut(s) 246
GluI GCNGC 7 cut(s) 326, 849, 1236, 1314, 2616, 2658, 2903
GsaI CCCAGC 1 cut(s) 2175
GsuI CTGGAG 4 cut(s) 1302, 1734, 2100, 2493
HaeIII GGCC 6 cut(s) 328, 642, 766, 1149, 2066, 3353
HgaI GACGC 1 cut(s) 2874
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 2111
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 2 cut(s) 55, 1625
HindII GTYRAC 2 cut(s) 55, 1625
HpaI GTTAAC 1 cut(s) 55
HphI GGTGA 7 cut(s) 8, 983, 1133, 1177, 1625, 1871, 2243
Hpy99I CGWCG 3 cut(s) 128, 271, 3254
HpyCH4IV ACGT 7 cut(s) 1280, 2111, 2395, 2447, 2545, 3178, 3386
HpyF10VI GCNNNNNNNGC 8 cut(s) 322, 334, 848, 2216, 2359, 2624, 2908, 3330
HpySE526I ACGT 7 cut(s) 1280, 2111, 2395, 2447, 2545, 3178, 3386
Hsp92I GRCGYC 1 cut(s) 2111
HspAI GCGC 1 cut(s) 245
KflI GGGWCCC 2 cut(s) 394, 2532
Kpn2I TCCGGA 2 cut(s) 433, 2593
Ksp22I TGATCA 1 cut(s) 1474
KspAI GTTAAC 1 cut(s) 55
LguI GCTCTTC 3 cut(s) 983, 1707, 3198
LmnI GCTCC 5 cut(s) 436, 1321, 1413, 2881, 3326
Lsp1109I GCAGC 5 cut(s) 835, 1247, 1300, 2627, 2669
MaeII ACGT 7 cut(s) 1280, 2111, 2395, 2447, 2545, 3178, 3386
MaeIII GTNAC 5 cut(s) 191, 774, 971, 1532, 1943
MfeI CAATTG 3 cut(s) 118, 929, 1116
MflI RGATCY 5 cut(s) 1456, 1684, 2003, 2144, 2648
MhlI GDGCHC 9 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364, 3004
MlsI TGGCCA 1 cut(s) 1149
MluI ACGCGT 1 cut(s) 1097
MluNI TGGCCA 1 cut(s) 1149
MlyI GAGTC 2 cut(s) 1001, 1046
MmeI TCCRAC 4 cut(s) 290, 1263, 3093, 3241
Mox20I TGGCCA 1 cut(s) 1149
Mph1103I ATGCAT 3 cut(s) 676, 2410, 2947
MroI TCCGGA 2 cut(s) 433, 2593
MroXI GAANNNNTTC 1 cut(s) 1795
MscI TGGCCA 1 cut(s) 1149
MslI CAYNNNNRTG 3 cut(s) 1227, 1446, 2450
Msp20I TGGCCA 1 cut(s) 1149
MspA1I CMGCKG 2 cut(s) 1238, 2660
MspR9I CCNGG 7 cut(s) 151, 322, 386, 387, 2333, 3335, 3379
MunI CAATTG 3 cut(s) 118, 929, 1116
Mva1269I GAATGC 2 cut(s) 676, 1432
MvaI CCWGG 2 cut(s) 322, 2333
MvnI CGCG 1 cut(s) 1099
MwoI GCNNNNNNNGC 8 cut(s) 322, 334, 848, 2216, 2359, 2624, 2908, 3330
NciI CCSGG 5 cut(s) 151, 386, 387, 3335, 3379
NcoI CCATGG 1 cut(s) 1659
NdeI CATATG 1 cut(s) 2875
NlaIV GGNNCC 8 cut(s) 36, 395, 396, 641, 1368, 1657, 2533, 2534
NmuCI GTSAC 4 cut(s) 191, 971, 1532, 1943
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 3 cut(s) 676, 2410, 2947
NspI RCATGY 6 cut(s) 374, 1785, 2000, 2192, 3298, 3333
NspV TTCGAA 1 cut(s) 2983
PaeI GCATGC 1 cut(s) 3333
PaeR7I CTCGAG 1 cut(s) 2038
PagI TCATGA 2 cut(s) 1396, 2050
PceI AGGCCT 1 cut(s) 3353
PciI ACATGT 1 cut(s) 2188
PciSI GCTCTTC 3 cut(s) 983, 1707, 3198
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 2392
PctI GAATGC 2 cut(s) 676, 1432
PdmI GAANNNNTTC 1 cut(s) 1795
PkrI GCNGC 7 cut(s) 327, 850, 1237, 1315, 2617, 2659, 2904
PleI GAGTC 2 cut(s) 1001, 1046
PmaCI CACGTG 2 cut(s) 2396, 2546
PmlI CACGTG 2 cut(s) 2396, 2546
PpsI GAGTC 2 cut(s) 1001, 1046
Ppu21I YACGTR 2 cut(s) 2396, 2546
PpuMI RGGWCCY 2 cut(s) 394, 2532
PscI ACATGT 1 cut(s) 2188
PshAI GACNNNNGTC 1 cut(s) 951
Psp124BI GAGCTC 3 cut(s) 293, 1418, 2364
Psp5II RGGWCCY 2 cut(s) 394, 2532
Psp6I CCWGG 2 cut(s) 320, 2331
PspCI CACGTG 2 cut(s) 2396, 2546
PspFI CCCAGC 1 cut(s) 2171
PspGI CCWGG 2 cut(s) 320, 2331
PspN4I GGNNCC 8 cut(s) 36, 395, 396, 641, 1368, 1657, 2533, 2534
PspPPI RGGWCCY 2 cut(s) 394, 2532
PspXI VCTCGAGB 1 cut(s) 2038
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2382, 2414
PstI CTGCAG 1 cut(s) 1165
PstNI CAGNNNCTG 1 cut(s) 3062
PsuI RGATCY 5 cut(s) 1456, 1684, 2003, 2144, 2648
PvuII CAGCTG 1 cut(s) 1238
RsaI GTAC 8 cut(s) 1509, 2193, 2382, 2391, 2461, 2664, 2763, 2824
RsaNI GTAC 8 cut(s) 1508, 2192, 2381, 2390, 2460, 2663, 2762, 2823
RseI CAYNNNNRTG 3 cut(s) 1227, 1446, 2450
SacI GAGCTC 3 cut(s) 293, 1418, 2364
SapI GCTCTTC 3 cut(s) 983, 1707, 3198
SatI GCNGC 7 cut(s) 326, 849, 1236, 1314, 2616, 2658, 2903
SchI GAGTC 2 cut(s) 1001, 1046
ScrFI CCNGG 7 cut(s) 151, 322, 386, 387, 2333, 3335, 3379
SduI GDGCHC 9 cut(s) 147, 158, 293, 730, 886, 1224, 1418, 2364, 3004
SfcI CTRYAG 1 cut(s) 1161
Sfr274I CTCGAG 1 cut(s) 2038
SfuI TTCGAA 1 cut(s) 2983
SinI GGWCC 8 cut(s) 34, 66, 394, 953, 2532, 2717, 2810, 3020
SlaI CTCGAG 1 cut(s) 2038
SmaI CCCGGG 1 cut(s) 387
SmiMI CAYNNNNRTG 3 cut(s) 1227, 1446, 2450
SmlI CTYRAG 1 cut(s) 2038
SmoI CTYRAG 1 cut(s) 2038
SpeI ACTAGT 2 cut(s) 1510, 3359
SphI GCATGC 1 cut(s) 3333
SseBI AGGCCT 1 cut(s) 3353
SsiI CCGC 9 cut(s) 38, 84, 325, 790, 1836, 2660, 2903, 3229, 3324
SspI AATATT 2 cut(s) 1343, 2683
SstI GAGCTC 3 cut(s) 293, 1418, 2364
StuI AGGCCT 1 cut(s) 3353
StyD4I CCNGG 7 cut(s) 149, 320, 384, 385, 2331, 3333, 3377
StyI CCWWGG 3 cut(s) 966, 1659, 3153
TaiI ACGT 7 cut(s) 1283, 2114, 2398, 2450, 2548, 3181, 3389
TaqI TCGA 5 cut(s) 2039, 2599, 2983, 2989, 3172
TaqII GACCGA 1 cut(s) 2482
TatI WGTACW 1 cut(s) 2191
TauI GCSGC 2 cut(s) 328, 2905
TseFI GTSAC 4 cut(s) 191, 971, 1532, 1943
TseI GCWGC 5 cut(s) 848, 1235, 1313, 2615, 2657
Tsp45I GTSAC 4 cut(s) 191, 971, 1532, 1943
TspGWI ACGGA 1 cut(s) 1000
TspMI CCCGGG 1 cut(s) 385
VneI GTGCAC 2 cut(s) 143, 882
VpaK11BI GGWCC 8 cut(s) 34, 66, 394, 953, 2532, 2717, 2810, 3020
XapI RAATTY 6 cut(s) 530, 935, 1110, 1561, 2014, 3031
XceI RCATGY 6 cut(s) 374, 1785, 2000, 2192, 3298, 3333
XcmI CCANNNNNNNNNTGG 2 cut(s) 1544, 1696
XhoI CTCGAG 1 cut(s) 2038
XmaI CCCGGG 1 cut(s) 385
XmiI GTMKAC 2 cut(s) 449, 757
XmnI GAANNNNTTC 1 cut(s) 1795
ZraI GACGTC 1 cut(s) 2112
Zsp2I ATGCAT 3 cut(s) 676, 2410, 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.