RchiOBHm_Chr7g0187521

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013452
Physical Location & Seq
Forward (+)
7333314 .. 7339034
5721 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16739

Sequence Viewer

Length: 3357 bp
ATGAAAGGCCGATCTCACCGGCTTCAGAGTTCGGACCCGCCGGACGACTGGGTGGATGAGTCGTGGACGGTGGATTGTTTGTGTGGTGTGACTTTCGACGATGGGGAAGAGATGGTGAATTGTGATGAGTGTGGTGTTTGGGTGCACACTCGGTGTTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGATAAATGTAAGAGCAGGAATAGTAGGAATGATAGTGAGGAAACTGAGGTTGCGCAATTGTTGGTTGAGCTGCCTACCAAGACTGTGAGAATGGAGAGTTCGTTTCCGCCACCACCGAATATGCCTACTCGGCGCCCTCTTAGGCTTTGGACTGATATACCAATGGAGGAGAGGGTGCATGTGCAGGGAATTCCAGGGGGTGACCCTGCTATATTTGGTGGCTTGTCCTCGGTTTTTACTCCGGAGCTGTGGAAGTCAACGGGATATGTGCCCAAAAAGTTTAATTTTCAGTATAGGGAGTTCCCTTGTTGGGATAAGAAGGAGGAGGCTGATGTTAGATTTGATGAAGACAGTGAGAATGCTGTGGATAAAGGTGCTGGCGTTCTGTTCTCTTTGTTGAATGAGAGTGTCTTGGCAAGCCCAGTGGCTGCTTTGGTTGGAATGAGGAGCCGAGAAGAAGGCGGGTATGACAAGAAGGTCTCTTTGAAAGAGACAAAAAGGTGGGATAAGGAGGTTCGTGATATTAGATGTGCTCAGAGTGGTGTGAAGAAGGAGAGGAGTTTACTGCGGCCCATGGTATTACATACGGGTAAGCGTAAGAAGGACGATCTGGGTACATCCAAAGATCGGAGTGCCAAGAAGAGGGCTAGAGCTACTGAGAAAGAGGCTGATGCAAAGAAGAGAGGGGCTCAATCTTCTAAATCAGTGTTTACACCCTCAAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATTTCTAAGGCTGATGTTCAGAGTATGAAGTACAAGAAGTCGAGCAACTCTGTGGTTAGAGAACCTGCACCAAATGTTTCTCTTGCGACAGACTGTACTGTTGAAAATCACTCTTCAGAAGCCTTACTTTCTGATAGGTCTAAAAAGATTGATGATGGGCTGAAGGAAGACAAGGTGGAACACCAAGTTTCTGCAGTGCCGGGGCACATGACTATCACCAAAATGGATGGTGCTGCTGTTGCATCATTGTTAGAATTAAATGATGCTAGGACTGATTGTCTACAAGAACAGGGAGATAGCACAGAAGATGAAAATTTGAATGTGAAACCACCCATTGAAAATGTTAGCACTGCGCCGGAGGTTGAAGTTCAGAATCACTGTCCTACTAGCGACGGGTCCGTGCAACGTTCTCCCAATAAGAAAACTGAAGACCATGAGGACAATTCTCAGAGCCCGGTGAATGTGCAGTCTTCTCTTCATGGTGAGGCAAAAGATCTCGGCAAATCTTCTGATCAAGTGTCTGAAAGTTTGAAACTTAATGGTGTAATAGTAAACGTCCCACTGTCCAGTGACCATAAGGTGCAAGGTGCTGACAGAACTTCTGAAGCAGTTAGTGATTCTCATACAGAGAGGGGTGATGAATTATCTGGCGATTGCCAGCCTAAACGGGAATCAGAAGATTTGGTGGATTCAGTTACTCTGCAAAAATGTTCTTCAGATGTCAAACATGGTTTGAAGCTTTCAGAAGATCTTTCAAAACCAGGCGGGATCTTAAACTCTGCAGCAGCATCGGGTCAGCTTAAAACAGCAAGTGCCGGGAAATCTTGTACTGTTCCATCCACCAGTTTGACCCCCAAATCATCCACTCCTCAGAACTTAAAATCTGGTGATGTTCAGAATCCTAATCCATTTACTAAGCAACAAGTAATGTCTGAAAGTAAAGCTAGCATTAAGAAAGATCGTGCTTCATCTGCGGACATGGATCACGATAAGGACAATATGCCAAGGAAAATAGTAAAAGAGCAGTTAAGATCCCCTACAAGTTCTGCTCTGAAAACCTCACACTTCAGCAGGAATTCACATGATTCTGTATCCAAGCGGACCACATCAGAATCAAAAGATTCATTGCTTCACTCATCTTCCAAGACATTGTCAGAAGGGGATACAGTTCCTTCAGGCTCTAGTGAGAAGGTTTTACATGGGCAGAACAAAAGTTCAGTGTCAAGTGCATTGCAAAGAGGTGAAAAGTTAAACCAGACAACTTCTTCTAAGACTAGTCAGAGTCATGCACCACCTTCTTGTCCACCTGCACCATCAAGTTCTCAAGCAAAACTAAGTGATGAGGAACTCGCTTTACTTTTACATCAAGAACTCAACAGTTCTCCCAGAGTACCTCGTGTACCACGTGCTCGTCATGCGTCTAGCTTACCTCAGCTGGCTTCTCCAACTGCAACAAGCATGTTAATAAAGCGTACATCTTCTTCTAGCGGAAAGGATCACAACTCGGGCTCCAGAAGAAAAGTGAGGGATGCATATAAAGATGGGGTCCGAAGTTCCCGTGAACTGGATGATGAAACTAAGAGGATGGACAGAGTCCCATCTTCTCCTGATCCTAGAAGACAAGATGCAGCTAGTACAGTAGATGCTACTAGGAGGGAGGAAAATGCATCTTCAACAGCCTCACATTCTTACAAAAAGACTATCCCTTCTACCTCCATTCCAACTGCAAACAGTGGTCCATCTTCATCCACTGAGGCTAATGATCGCAATGTGTCATCAGTACGCAGTTCACCGAGGAATGCGTCTGACGATGATACAGGGGCAGTTGGTCCTGTTCATCCTACTTTACCAGGCTTGATAAATGAGATTATGAGTAAAGGTAGACGAATGACGTATGAGGAGCTCTGCAATGCTGTGATGCCGCACTGGCATAATCTGAGAAAGCATAATGGAGAGCGTTATGCATATACCAGTCCTTCGCAGGCTGTTCTTGATTGCCTTCGGAATCGACATGAATGGGCTCGATTAGTTGATCGTGGTCCAAAGACAAACCCTAGGAAAAAGCGAAAACTTGATGCTGATGATTCAGAGGATAATGAATATGGCAGGGTCAACAATCCAAAGGAGTTGGAAGGTAAAAGCATAGAGACACAGAGAGAGGACTATCCAAAGGGCAAGAGAAAAGCTCGAAAACGCAGAAGACTGGCCCTTCAAGGAAGAGGAATAAAGGATGTCAGGGAGAAACGGAAGGCAGATTTGCTCACCGATGATGATGTAGGGCCATCATTTTCTAATTCCACAGAGGAAACTGTGTCTGAGGATGATACTCAGGGAGGTGGAGCAGGGGGTCCAGTTGGAAGTGAGGCCTCTTCTAGTTCAGAGGAGGAAGGGACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1118

Amino Acids

122.43

Weight (kDa)

7.15

Isoelectric Point (pI)

58.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 934 - 996 4.8e-34 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2272
AasI GACNNNNNNGTC 1 cut(s) 668
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 2 cut(s) 1030, 2272
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 2 cut(s) 1237, 2839
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 9 cut(s) 38, 299, 654, 760, 1722, 1931, 2056, 2445, 2879
AclI AACGTT 1 cut(s) 1363
AclWI GGATC 5 cut(s) 1733, 1947, 1983, 2460, 2561
AcsI RAATTY 4 cut(s) 381, 932, 1270, 2032
AcuI CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2115
AcvI CACGTG 1 cut(s) 2363
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 9 cut(s) 808, 989, 1054, 1786, 2349, 2358, 2431, 2593, 2739
AfiI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2521
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 2231
AjnI CCWGG 3 cut(s) 385, 1717, 2806
Alw21I GWGCWC 4 cut(s) 147, 727, 2368, 2862
Alw26I GTCTC 3 cut(s) 676, 677, 3098
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 5 cut(s) 1733, 1947, 1983, 2460, 2561
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 2461
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 5 cut(s) 7, 761, 3162, 3236, 3321
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 6 cut(s) 262, 620, 1190, 1739, 1742, 2585
ApoI RAATTY 4 cut(s) 381, 932, 1270, 2032
AspA2I CCTAGG 1 cut(s) 3011
AspLEI GCGC 3 cut(s) 247, 327, 1312
AsuC2I CCSGG 3 cut(s) 1158, 1412, 1774
AsuNHI GCTAGC 1 cut(s) 1901
AvaI CYCGRG 1 cut(s) 2461
AvaII GGWCC 9 cut(s) 34, 950, 1353, 2058, 2503, 2693, 2786, 2996, 3305
AvrII CCTAGG 1 cut(s) 3011
BaeGI GKGCMC 3 cut(s) 147, 465, 1164
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 5 cut(s) 883, 1412, 2468, 2862, 2980
BarI GAAGNNNNNNTAC 2 cut(s) 2422, 2454
BauI CACGAG 1 cut(s) 2352
BbrPI CACGTG 1 cut(s) 2363
BbsI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2581, 3163
Bbv12I GWGCWC 4 cut(s) 147, 727, 2368, 2862
BbvCI CCTCAGC 1 cut(s) 2388
BbvI GCAGC 6 cut(s) 249, 607, 1177, 1751, 1754, 2597
BcgI CGANNNNNNTGC 2 cut(s) 1728, 1762
BciT130I CCWGG 3 cut(s) 387, 1719, 2808
BciVI GTATCC 2 cut(s) 2059, 2113
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 3 cut(s) 1158, 1412, 1774
BcoDI GTCTC 3 cut(s) 676, 677, 3098
BcuI ACTAGT 1 cut(s) 2231
BfmI CTRYAG 2 cut(s) 1149, 1737
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 2 cut(s) 1030, 2272
BfuI GTATCC 2 cut(s) 2059, 2113
BglI GCCNNNNNGGC 2 cut(s) 322, 2884
BglII AGATCT 2 cut(s) 1450, 1705
BisI GCNGC 8 cut(s) 263, 621, 761, 1191, 1740, 1743, 2586, 2879
BlnI CCTAGG 1 cut(s) 3011
BlsI GCNGC 8 cut(s) 264, 622, 762, 1192, 1741, 1744, 2587, 2880
Bme1390I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2808
Bme18I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2503, 2693, 2786, 2996, 3305
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 1 cut(s) 2461
BmiI GGNNCC 7 cut(s) 36, 326, 641, 1354, 2467, 2504, 3306
BmrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2808
BmrI ACTGGG 2 cut(s) 58, 608
BmtI GCTAGC 1 cut(s) 1905
BmuI ACTGGG 2 cut(s) 58, 608
BoxI GACNNNNGTC 1 cut(s) 948
BpiI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2581, 3163
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 3127, 3159
BpmI CTGGAG 1 cut(s) 2452
Bpu10I CCTNAGC 1 cut(s) 2388
BpuEI CTTGAG 2 cut(s) 895, 2265
BpuMI CCSGG 3 cut(s) 1158, 1412, 1774
BsaAI YACGTR 1 cut(s) 2363
BsaHI GRCGYC 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2750, 3011
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 2450, 2480
Bsc4I CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2521
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 9 cut(s) 53, 614, 1524, 1800, 2526, 2888, 2928, 3165, 3308
Bse3DI GCAATG 4 cut(s) 2081, 2186, 2731, 2872
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 3 cut(s) 387, 1719, 2808
BseDI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2750, 3011
BseLI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2521
BseMI GCAATG 4 cut(s) 2081, 2186, 2731, 2872
BseNI ACTGG 9 cut(s) 53, 614, 1524, 1800, 2526, 2888, 2928, 3165, 3308
BseRI GAGGAG 7 cut(s) 374, 530, 652, 763, 1815, 2870, 3353
BseSI GKGCMC 3 cut(s) 147, 465, 1164
BseXI GCAGC 6 cut(s) 249, 607, 1177, 1751, 1754, 2597
BsgI GTGCAG 4 cut(s) 395, 1008, 1442, 2250
BshFI GGCC 5 cut(s) 9, 763, 3164, 3238, 3323
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 4 cut(s) 147, 727, 2368, 2862
BsiHKCI CYCGRG 1 cut(s) 2461
BsiSI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
BslFI GGGAC 2 cut(s) 1499, 2537
BslI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2521
BsmAI GTCTC 3 cut(s) 676, 677, 3098
BsmFI GGGAC 2 cut(s) 1499, 2537
BsmI GAATGC 2 cut(s) 556, 2761
BsnI GGCC 5 cut(s) 9, 763, 3164, 3238, 3323
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 2461
Bsp13I TCCGGA 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 9 cut(s) 38, 299, 654, 760, 1722, 1931, 2056, 2445, 2879
BspANI GGCC 5 cut(s) 9, 763, 3164, 3238, 3323
BspEI TCCGGA 1 cut(s) 433
BspLI GGNNCC 7 cut(s) 36, 326, 641, 1354, 2467, 2504, 3306
BspMAI CTGCAG 2 cut(s) 1153, 1741
BspMI ACCTGC 2 cut(s) 1030, 2272
BspOI GCTAGC 1 cut(s) 1905
BspPI GGATC 5 cut(s) 1733, 1947, 1983, 2460, 2561
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 676
BsrDI GCAATG 4 cut(s) 2081, 2186, 2731, 2872
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 9 cut(s) 53, 614, 1524, 1800, 2526, 2888, 2928, 3165, 3308
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 7 cut(s) 386, 420, 765, 1157, 1961, 2750, 3011
BssNI GRCGYC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 2352
BssT1I CCWWGG 3 cut(s) 765, 1961, 3011
Bst2BI CACGAG 1 cut(s) 2352
Bst2UI CCWGG 3 cut(s) 387, 1719, 2808
Bst6I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3169, 3331
BstACI GRCGYC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 2363
BstC8I GCNNGC 6 cut(s) 571, 610, 1616, 1903, 2394, 2940
BstDSI CCRYGG 1 cut(s) 765
BstEII GGTNACC 1 cut(s) 392
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 3 cut(s) 247, 327, 1312
BstMAI GTCTC 3 cut(s) 676, 677, 3098
BstMWI GCNNNNNNNGC 5 cut(s) 322, 1196, 1928, 2372, 2884
BstNI CCWGG 3 cut(s) 387, 1719, 2808
BstNSI RCATGY 2 cut(s) 374, 2419
BstPAI GACNNNNGTC 1 cut(s) 948
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1772, 2806
BstSFI CTRYAG 2 cut(s) 1149, 1737
BstSLI GKGCMC 3 cut(s) 147, 465, 1164
BstV1I GCAGC 6 cut(s) 249, 607, 1177, 1751, 1754, 2597
BstV2I GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 2581, 3163
BstX2I RGATCY 4 cut(s) 1450, 1705, 1725, 1988
BstYI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
BsuI GTATCC 2 cut(s) 2059, 2113
BsuRI GGCC 5 cut(s) 9, 763, 3164, 3238, 3323
BtgI CCRYGG 1 cut(s) 765
BtsI GCAGTG 2 cut(s) 1158, 1305
BveI ACCTGC 2 cut(s) 1030, 2272
Cac8I GCNNGC 6 cut(s) 571, 610, 1616, 1903, 2394, 2940
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 3 cut(s) 247, 327, 1312
Cfr10I RCCGGY 1 cut(s) 18
CseI GACGC 2 cut(s) 2364, 2748
Csp6I GTAC 9 cut(s) 807, 988, 1053, 1785, 2348, 2357, 2430, 2592, 2738
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
CviQI GTAC 9 cut(s) 807, 988, 1053, 1785, 2348, 2357, 2430, 2592, 2738
DinI GGCGCC 1 cut(s) 326
DraIII CACNNNGTG 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 668
DriI GACNNNNNGTC 1 cut(s) 1233
DseDI GACNNNNNNGTC 1 cut(s) 668
Eam1104I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3169, 3331
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3169, 3331
EciI GGCGGA 1 cut(s) 288
Ecl136II GAGCTC 1 cut(s) 2860
Eco130I CCWWGG 3 cut(s) 765, 1961, 3011
Eco147I AGGCCT 1 cut(s) 3323
Eco24I GRGCYC 5 cut(s) 883, 1412, 2468, 2862, 2980
Eco31I GGTCTC 1 cut(s) 676
Eco47I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2503, 2693, 2786, 2996, 3305
Eco53kI GAGCTC 1 cut(s) 2860
Eco57I CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2115
Eco72I CACGTG 1 cut(s) 2363
Eco88I CYCGRG 1 cut(s) 2461
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 2860
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 381, 2032
EcoRII CCWGG 3 cut(s) 385, 1717, 2806
EcoT14I CCWWGG 3 cut(s) 765, 1961, 3011
EcoT22I ATGCAT 3 cut(s) 2491, 2626, 2923
EcoT38I GRGCYC 5 cut(s) 883, 1412, 2468, 2862, 2980
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 3 cut(s) 765, 1961, 3011
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 2 cut(s) 1499, 2537
FauI CCCGC 3 cut(s) 45, 647, 1715
FbaI TGATCA 1 cut(s) 1468
FblI GTMKAC 2 cut(s) 1237, 2839
Fnu4HI GCNGC 8 cut(s) 263, 621, 761, 1191, 1740, 1743, 2586, 2879
FriOI GRGCYC 5 cut(s) 883, 1412, 2468, 2862, 2980
Fsp4HI GCNGC 8 cut(s) 263, 621, 761, 1191, 1740, 1743, 2586, 2879
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 3 cut(s) 246, 326, 1311
GluI GCNGC 8 cut(s) 263, 621, 761, 1191, 1740, 1743, 2586, 2879
GsuI CTGGAG 1 cut(s) 2452
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 5 cut(s) 9, 763, 3164, 3238, 3323
HapII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
HgaI GACGC 2 cut(s) 2364, 2748
HhaI GCGC 3 cut(s) 247, 327, 1312
Hin1I GRCGYC 1 cut(s) 325
Hin6I GCGC 3 cut(s) 245, 325, 1310
HinP1I GCGC 3 cut(s) 245, 325, 1310
HincII GTYRAC 2 cut(s) 450, 3070
HindII GTYRAC 2 cut(s) 450, 3070
HindIII AAGCTT 1 cut(s) 1694
HpaII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
Hpy188III TCNNGA 7 cut(s) 434, 710, 1943, 2324, 2469, 2564, 2948
Hpy99I CGWCG 2 cut(s) 101, 1352
HpyCH4IV ACGT 4 cut(s) 1363, 1512, 2362, 2849
HpyF10VI GCNNNNNNNGC 5 cut(s) 322, 1196, 1928, 2372, 2884
HpySE526I ACGT 4 cut(s) 1363, 1512, 2362, 2849
Hsp92I GRCGYC 1 cut(s) 325
HspAI GCGC 3 cut(s) 245, 325, 1310
KasI GGCGCC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 1468
LmnI GCTCC 5 cut(s) 436, 639, 2471, 2857, 3296
Lsp1109I GCAGC 6 cut(s) 249, 607, 1177, 1751, 1754, 2597
MaeII ACGT 4 cut(s) 1363, 1512, 2362, 2849
MaeIII GTNAC 4 cut(s) 88, 392, 1526, 1651
MfeI CAATTG 2 cut(s) 248, 926
MflI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 3 cut(s) 68, 2248, 2559
MmeI TCCRAC 5 cut(s) 610, 2426, 2702, 3066, 3292
Mph1103I ATGCAT 3 cut(s) 2491, 2626, 2923
MroI TCCGGA 1 cut(s) 433
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 2392
MspI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1773
MspR9I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2808
MunI CAATTG 2 cut(s) 248, 926
Mva1269I GAATGC 2 cut(s) 556, 2761
MvaI CCWGG 3 cut(s) 387, 1719, 2808
MwoI GCNNNNNNNGC 5 cut(s) 322, 1196, 1928, 2372, 2884
NarI GGCGCC 1 cut(s) 325
NciI CCSGG 3 cut(s) 1158, 1412, 1774
NcoI CCATGG 1 cut(s) 765
NheI GCTAGC 1 cut(s) 1901
NlaIV GGNNCC 7 cut(s) 36, 326, 641, 1354, 2467, 2504, 3306
NmeAIII GCCGAG 3 cut(s) 301, 668, 1434
NmuCI GTSAC 3 cut(s) 88, 392, 1526
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 3 cut(s) 2491, 2626, 2923
NspI RCATGY 2 cut(s) 374, 2419
PaqCI CACCTGC 1 cut(s) 2272
PceI AGGCCT 1 cut(s) 3323
PcsI WCGNNNNNNNCGW 1 cut(s) 2359
PctI GAATGC 2 cut(s) 556, 2761
PflFI GACNNNGTC 2 cut(s) 2107, 2549
PkrI GCNGC 8 cut(s) 264, 622, 762, 1192, 1741, 1744, 2587, 2880
PleI GAGTC 3 cut(s) 67, 2247, 2558
PluTI GGCGCC 1 cut(s) 328
PmaCI CACGTG 1 cut(s) 2363
PmlI CACGTG 1 cut(s) 2363
PpsI GAGTC 3 cut(s) 67, 2247, 2558
Ppu21I YACGTR 1 cut(s) 2363
PshAI GACNNNNGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 2862
Psp1406I AACGTT 1 cut(s) 1363
Psp6I CCWGG 3 cut(s) 385, 1717, 2806
PspCI CACGTG 1 cut(s) 2363
PspEI GGTNACC 1 cut(s) 392
PspGI CCWGG 3 cut(s) 385, 1717, 2806
PspN4I GGNNCC 7 cut(s) 36, 326, 641, 1354, 2467, 2504, 3306
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2295, 2327
PstI CTGCAG 2 cut(s) 1153, 1741
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 4 cut(s) 1450, 1705, 1725, 1988
PsyI GACNNNGTC 2 cut(s) 2107, 2549
PvuII CAGCTG 1 cut(s) 2392
RsaI GTAC 9 cut(s) 808, 989, 1054, 1786, 2349, 2358, 2431, 2593, 2739
RsaNI GTAC 9 cut(s) 807, 988, 1053, 1785, 2348, 2357, 2430, 2592, 2738
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 2862
SatI GCNGC 8 cut(s) 263, 621, 761, 1191, 1740, 1743, 2586, 2879
SchI GAGTC 3 cut(s) 68, 2248, 2559
ScrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1774, 2808
SfcI CTRYAG 2 cut(s) 1149, 1737
SfoI GGCGCC 1 cut(s) 326
SinI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2503, 2693, 2786, 2996, 3305
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 910, 2280
SmoI CTYRAG 2 cut(s) 910, 2280
SpeI ACTAGT 1 cut(s) 2231
SseBI AGGCCT 1 cut(s) 3323
SsiI CCGC 9 cut(s) 38, 299, 654, 760, 1722, 1931, 2056, 2445, 2879
SspDI GGCGCC 1 cut(s) 324
SstI GAGCTC 1 cut(s) 2862
StuI AGGCCT 1 cut(s) 3323
StyD4I CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1772, 2806
StyI CCWWGG 3 cut(s) 765, 1961, 3011
TaiI ACGT 4 cut(s) 1366, 1515, 2365, 2852
TaqI TCGA 5 cut(s) 96, 998, 2965, 2980, 3145
TatI WGTACW 4 cut(s) 987, 1052, 1784, 2591
TauI GCSGC 2 cut(s) 763, 2881
TseFI GTSAC 3 cut(s) 88, 392, 1526
TseI GCWGC 6 cut(s) 262, 620, 1190, 1739, 1742, 2585
Tsp45I GTSAC 3 cut(s) 88, 392, 1526
TspGWI ACGGA 2 cut(s) 1345, 3217
Tth111I GACNNNGTC 2 cut(s) 2107, 2549
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2503, 2693, 2786, 2996, 3305
XapI RAATTY 4 cut(s) 381, 932, 1270, 2032
XceI RCATGY 2 cut(s) 374, 2419
XmaJI CCTAGG 1 cut(s) 3011
XmiI GTMKAC 2 cut(s) 1237, 2839
Zsp2I ATGCAT 3 cut(s) 2491, 2626, 2923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.