Rroxscaffold_3G00267360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013452
Physical Location & Seq
Reverse (-)
60408833 .. 60414574
5742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267360.1

Sequence Viewer

Length: 3291 bp
ATGAAAGGCCGATCTCACCGGCTTCAGAGTTCGGACCCGCCGGACGACTGGGTGGATGAGTCGTGGACGGTGGATTGTTTGTGTGGTGTGACTTTCGACGATGGGGAAGAGATGGTGAATTGTGATGAGTGTGGTGTTTGGGTGCACACTCGGTGTTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGATAAATGTAAGAGCAGAAATAGTAGGAATGATAGTGAGGAAACTGAGGTTGCGCAATTGTTGGTTGAGCTGCCTACCAAGACTGTGAGAATGGAGAGTTCGTTTCCGCCACCACCGAATATGCCTACTCGGCGCCCTCTTAGGCTTTGGACTGATATACCGATGGAGGAGAGGGTGCATGTGCAGGGAATTCCAGGGGGTGACCCTGCTATATTTGGTGGGTTGTCGTCGGTTTTTACTCCGGAGCTGTGGAAGTCGACGGGATATGTGCCCAAAAAGTTTAATTTTCAGTATAGGGAGTTCCCTTGTTGGGATAAGAAGGAGGAGGCTGATGTTAGATTTGATGAAGACAGTGAGAATGCTGTGGATAAAGGTGCTGGCGTTCTGTTCTCTTTGTTGAATGAGAGTGTCTTGGCAAGCCCAGTGGCTGCTTTGGTTGGAATGAGGAGCCGAGAAGAAGGCGGGTATGACAAGAAGGTCTCTTTGAAAGAGACAAAAAGGTGGGATAAGGAGGTTCGTGATCTTAGATGTGCTCAGAGTGGTGTGAAGAAGGAGAGAAGTTTACTGCGGCCCATGGTATTACATACGGGTAAGCGTAAGAAGGACGATCTGGGTACATCCAAAGATCGGAGTGCCAAGAAGAGGGCTAGAGCTGCTGAGAAAGAGTCTGATGCAAAGAAGAGAGGGGCTCAATCTTCTAAATCAGTGTTTACACCCTCAAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATATCTAAGGCTGATGTTCAGAGTATGAAGTACAAGAAGTCGAGCAACTCTGTGGTTAGAGAACCTGCACCAAATGTTTCTCTTGCGACAGACTGTACTGTTGAAAATCACTCTTCAGAAGCCTTACTTTCTGATAGGTCTAAAAAGATTGATGATGGGCTGAAGGAAGACAAGGTGGAACACCAAGTTTCTGCAGTGCCGGGGCACATGACTATCACCAACATGGATGGTGCTGCTGTTGCATCATTGTTAGAATTAAATGATGCTAGGACTGATTGTCTACAAGAACAGGGAGATAGCACAGAAGATGAAAATTTGAATGTGAAACCACCCATTGAAAATGTTAGCACTGCACCGGAGGTTGAAGTTCAGAATCACTGTCCTACTAGCGACGGGTCCGTACAACGTTCTCCCAATAAGAAAACTGAAGACCATGAGGACAATTCTCAGAGCCCGGTGAATGTGCAGTCTTCTCTTCACGGTGAGGCAAAAGATCTCGGCAAATCTTCTGATCAAGTGTCTGAAAGTTCGAAACTTAATGGGGGTGATGAATTATCTGGCGATTGCCAGCCTAAACGGGAATCAGAAGATTTGGCGGATTCAGTTACTCTGCAAAAATGTTCTTCAGATGTCAAACATGGTTTGAAGCTTTCAGAAGATCTTTCAAAACCAGGCGGGATCTTAAACTCTGCAGCAGCACCGGGTCAGCTTAAAACAGCAAGTGCTGGGAAATCTTGTACTGTTCCATCCACCAGTTTGACCCCCAAATCATCCACTCCTCATAACTTAAAATCTGGTGATGTTCAGAATCCTAATCCATTTACTAAGCAACGAGTAATGTCTGAAAGTAAAGTTAGCATTAAGAAAGATCGTGCTTCATCTGCGGACATGGATCGCGATAAGGACAATATGCCAAGGAAAATAGTAAAAGAGCAGTTAAGATCCCCTACAAGTTCTGCTCTGAAAACCTCACACTTCAGCAGGAATTCACATGATTCTGTATCCAAGCGGACCACATCAGAATCAAAAGATTCATTGCTTCACTCATCTTCCAAGACATTGTCAGAAGGGGATACTGCAGTTCCTTCAGGCTCTAGTGAGAAGGTTTTACATGGGCAGAACAAAAGTTCAGCGTCAAGTGCATTGCAAAGAGGTGAAAAGTTAAACCAGACAACTTCTTCTAAGACTAGTCAGAGTCATGCACCACCTTCTTGTCCACCTGCACCATCAAGTTCTCAAGCAAAACTAAGTGATGAGGAACTTGCTTTACTTTTACATCAAGAACTCAACAGTTCTCCCAGAGTACCTCGTGTACCACGTGCTCGTCATGCGTCTAGCTTACCTCAGCTGTCTTCGCCAACTGCAACAAGCATGTTAATAAAGCGTACATCTTCTTCTAGCGGAAAGGATCACAACTCGGGCTCTAGAAGAAAAGTGAGGGATGCATATAAAGATGGGGTCCGAAGTTCCCGTGAACTGGATGATGAAACTAAGAGGATGGACAGAGTCCCATCTTCTCCTGATCCTAGAAGACAAGACGCAGCTAGTACAGTAGATGCTACTAGGAGGGAGGAAAATGCATCTTCAACAGCCTCACATTCTTACAAAAAGACTATCCCTTCTACCTCCATTCCAACTGCAAACAGTGGTCCATCTTCATCCACCGAGGCTAATGATCGCAATGTGTCATCAGTACGCAGTTCACCGAGGAATGCATCTGACGATGATACAGGGGCAGTTGGTCCAGTTCATCCTACTTTACCAGGCTTGATAAATGAGATTATGAGTAAAGGTAGACGAATGACGTATGAGGAGCTCTGCAATGCTGTGTTGCCGCACTGGCATAATCTGAGAAAGCACAATGGAGAGCGTTATGCATATACAAGTCCTTCGCAGGCTGTTCTTGATTGCCTTCGGAATCGACATGAATGGGCTCGATTAGTTGATCGTGGTCCGAAGACAAACCCTAGGAAAAAGCGAAAACTTGATGCTGATGATTCAGAGGATAATGAATATGGCAGGGTCAACAATCCAAAGGAGTTGGAAGGTAAAAGCATAGAGACACAGAGAGAGGACTATCCAAAGGGCAAGAGAAAAGCTCGAAAACGCAGAAGACTGGCCCTTCAAGGAAGAGGAATAAAGGATGTCAGGGAGAAACGGAAGGCAGATTTGCTCACCGATGATGATGTAGGGCCATCATTTTCTAATTCCACAGAGGAAACTGTGTCTGAGGATGATATTCAGGGGGTGGAGCAGGTCCAGTTGGAAGTGAGGCCTCTTCTAGTTCAGAGGAGGAAGGGACAAGTTGATTGTCCAGTGTCTGGTCTGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1096

Amino Acids

120.59

Weight (kDa)

8.38

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 905 - 967 3.7e-34 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2185
AasI GACNNNNNNGTC 1 cut(s) 668
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 3 cut(s) 1030, 2185, 3202
AccB1I GGYRCC 1 cut(s) 324
AccB7I CCANNNNNTGG 1 cut(s) 3278
AccI GTMKAC 3 cut(s) 449, 1237, 2752
AccII CGCG 1 cut(s) 1854
AccIII TCCGGA 1 cut(s) 433
AclI AACGTT 1 cut(s) 1363
AclWI GGATC 5 cut(s) 1643, 1857, 1893, 2373, 2474
AcsI RAATTY 4 cut(s) 381, 932, 1270, 1942
AcuI CTGAAG 7 cut(s) 8, 1056, 1139, 1404, 1566, 1918, 2028
AcvI CACGTG 1 cut(s) 2276
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 153
AfiI CCNNNNNNNGG 5 cut(s) 502, 819, 834, 2434, 3278
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 2144
AjnI CCWGG 3 cut(s) 385, 1627, 2719
Alw21I GWGCWC 4 cut(s) 147, 727, 2281, 2775
Alw26I GTCTC 3 cut(s) 676, 677, 3011
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 5 cut(s) 1643, 1857, 1893, 2373, 2474
AlwNI CAGNNNCTG 2 cut(s) 620, 3278
Ama87I CYCGRG 1 cut(s) 2374
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 5 cut(s) 7, 761, 3075, 3149, 3230
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 7 cut(s) 262, 620, 845, 1190, 1649, 1652, 2498
ApoI RAATTY 4 cut(s) 381, 932, 1270, 1942
AspA2I CCTAGG 1 cut(s) 2924
AspLEI GCGC 2 cut(s) 247, 327
AsuC2I CCSGG 3 cut(s) 1158, 1412, 1659
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 1 cut(s) 2374
AvaII GGWCC 9 cut(s) 34, 950, 1353, 1968, 2416, 2606, 2699, 2909, 3214
AvrII CCTAGG 1 cut(s) 2924
BaeGI GKGCMC 3 cut(s) 147, 465, 1164
BaeI ACNNNNGTAYC 2 cut(s) 2022, 2055
BanI GGYRCC 1 cut(s) 324
BanII GRGCYC 5 cut(s) 883, 1412, 2381, 2775, 2893
BarI GAAGNNNNNNTAC 2 cut(s) 2335, 2367
BauI CACGAG 1 cut(s) 2265
BbrPI CACGTG 1 cut(s) 2276
BbsI GAAGAC 8 cut(s) 546, 1131, 1392, 1419, 2301, 2494, 2921, 3076
Bbv12I GWGCWC 4 cut(s) 147, 727, 2281, 2775
BbvCI CCTCAGC 1 cut(s) 2301
BbvI GCAGC 7 cut(s) 249, 607, 832, 1177, 1661, 1664, 2510
BciT130I CCWGG 3 cut(s) 387, 1629, 2721
BciVI GTATCC 2 cut(s) 1969, 2023
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 3 cut(s) 1158, 1412, 1659
BcoDI GTCTC 3 cut(s) 676, 677, 3011
BcuI ACTAGT 1 cut(s) 2144
BfmI CTRYAG 3 cut(s) 1149, 1647, 2034
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 3 cut(s) 1030, 2185, 3202
BfuI GTATCC 2 cut(s) 1969, 2023
BglI GCCNNNNNGGC 2 cut(s) 322, 2797
BglII AGATCT 2 cut(s) 1450, 1615
BisI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1650, 1653, 2499, 2792
BlnI CCTAGG 1 cut(s) 2924
BlsI GCNGC 9 cut(s) 264, 622, 762, 847, 1192, 1651, 1654, 2500, 2793
Bme1390I CCNGG 6 cut(s) 387, 1158, 1412, 1629, 1659, 2721
Bme18I GGWCC 9 cut(s) 34, 950, 1353, 1968, 2416, 2606, 2699, 2909, 3214
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 1 cut(s) 2374
BmiI GGNNCC 5 cut(s) 36, 326, 641, 1354, 2417
BmrFI CCNGG 6 cut(s) 387, 1158, 1412, 1629, 1659, 2721
BmrI ACTGGG 2 cut(s) 58, 608
BmsI GCATC 9 cut(s) 853, 907, 1208, 1210, 2389, 2503, 2546, 2681, 2935
BmuI ACTGGG 2 cut(s) 58, 608
BoxI GACNNNNGTC 1 cut(s) 948
BpiI GAAGAC 8 cut(s) 546, 1131, 1392, 1419, 2301, 2494, 2921, 3076
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 3040, 3072
Bpu10I CCTNAGC 1 cut(s) 2301
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 2 cut(s) 895, 2178
BpuMI CCSGG 3 cut(s) 1158, 1412, 1659
BsaAI YACGTR 1 cut(s) 2276
BsaHI GRCGYC 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 7 cut(s) 386, 765, 1157, 1871, 2622, 2663, 2924
BsaWI WCCGGW 2 cut(s) 433, 1312
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 5 cut(s) 502, 819, 834, 2434, 3278
Bse118I RCCGGY 1 cut(s) 18
Bse1I ACTGG 9 cut(s) 53, 614, 1710, 2439, 2702, 2801, 3078, 3217, 3272
Bse3DI GCAATG 4 cut(s) 1991, 2099, 2644, 2785
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 3 cut(s) 387, 1629, 2721
BseDI CCNNGG 7 cut(s) 386, 765, 1157, 1871, 2622, 2663, 2924
BseLI CCNNNNNNNGG 5 cut(s) 502, 819, 834, 2434, 3278
BseMI GCAATG 4 cut(s) 1991, 2099, 2644, 2785
BseMII CTCAG 8 cut(s) 228, 740, 840, 930, 1418, 2315, 2798, 3177
BseNI ACTGG 9 cut(s) 53, 614, 1710, 2439, 2702, 2801, 3078, 3217, 3272
BseRI GAGGAG 6 cut(s) 374, 530, 652, 1725, 2783, 3262
BseSI GKGCMC 3 cut(s) 147, 465, 1164
BseXI GCAGC 7 cut(s) 249, 607, 832, 1177, 1661, 1664, 2510
BseYI CCCAGC 1 cut(s) 1682
BsgI GTGCAG 5 cut(s) 395, 1008, 1293, 1442, 2163
Bsh1236I CGCG 1 cut(s) 1854
BshFI GGCC 5 cut(s) 9, 763, 3077, 3151, 3232
BshNI GGYRCC 1 cut(s) 324
BsiHKAI GWGCWC 4 cut(s) 147, 727, 2281, 2775
BsiHKCI CYCGRG 1 cut(s) 2374
BsiSI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1658
BslFI GGGAC 2 cut(s) 2450, 3270
BslI CCNNNNNNNGG 5 cut(s) 502, 819, 834, 2434, 3278
BsmAI GTCTC 3 cut(s) 676, 677, 3011
BsmFI GGGAC 2 cut(s) 2450, 3270
BsmI GAATGC 2 cut(s) 556, 2674
BsnI GGCC 5 cut(s) 9, 763, 3077, 3151, 3232
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 2374
Bsp119I TTCGAA 1 cut(s) 1487
Bsp13I TCCGGA 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 765
Bsp68I TCGCGA 1 cut(s) 1854
BspANI GGCC 5 cut(s) 9, 763, 3077, 3151, 3232
BspCNI CTCAG 8 cut(s) 229, 739, 841, 931, 1417, 2314, 2799, 3178
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 1 cut(s) 1854
BspLI GGNNCC 5 cut(s) 36, 326, 641, 1354, 2417
BspMAI CTGCAG 3 cut(s) 1153, 1651, 2038
BspMI ACCTGC 3 cut(s) 1030, 2185, 3202
BspPI GGATC 5 cut(s) 1643, 1857, 1893, 2373, 2474
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 676
BsrDI GCAATG 4 cut(s) 1991, 2099, 2644, 2785
BsrFI RCCGGY 1 cut(s) 18
BsrI ACTGG 9 cut(s) 53, 614, 1710, 2439, 2702, 2801, 3078, 3217, 3272
BssAI RCCGGY 1 cut(s) 18
BssECI CCNNGG 7 cut(s) 386, 765, 1157, 1871, 2622, 2663, 2924
BssNI GRCGYC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 2265
BssT1I CCWWGG 3 cut(s) 765, 1871, 2924
Bst2BI CACGAG 1 cut(s) 2265
Bst2UI CCWGG 3 cut(s) 387, 1629, 2721
Bst6I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3082, 3240
BstACI GRCGYC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 2276
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 4 cut(s) 571, 610, 1526, 2853
BstDSI CCRYGG 1 cut(s) 765
BstEII GGTNACC 1 cut(s) 392
BstFNI CGCG 1 cut(s) 1854
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 2 cut(s) 247, 327
BstMAI GTCTC 3 cut(s) 676, 677, 3011
BstMWI GCNNNNNNNGC 8 cut(s) 322, 845, 1196, 1838, 2096, 2285, 2311, 2797
BstNI CCWGG 3 cut(s) 387, 1629, 2721
BstNSI RCATGY 2 cut(s) 374, 2332
BstPAI GACNNNNGTC 1 cut(s) 948
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 6 cut(s) 385, 1156, 1410, 1627, 1657, 2719
BstSFI CTRYAG 3 cut(s) 1149, 1647, 2034
BstSLI GKGCMC 3 cut(s) 147, 465, 1164
BstUI CGCG 1 cut(s) 1854
BstV1I GCAGC 7 cut(s) 249, 607, 832, 1177, 1661, 1664, 2510
BstV2I GAAGAC 8 cut(s) 546, 1131, 1392, 1419, 2301, 2494, 2921, 3076
BstX2I RGATCY 4 cut(s) 1450, 1615, 1635, 1898
BstYI RGATCY 4 cut(s) 1450, 1615, 1635, 1898
BsuI GTATCC 2 cut(s) 1969, 2023
BsuRI GGCC 5 cut(s) 9, 763, 3077, 3151, 3232
BtgI CCRYGG 1 cut(s) 765
BtsI GCAGTG 2 cut(s) 1158, 1305
BtsIMutI CAGTG 9 cut(s) 550, 621, 903, 1158, 1305, 1333, 2608, 2794, 3279
BtuMI TCGCGA 1 cut(s) 1854
BveI ACCTGC 3 cut(s) 1030, 2185, 3202
Cac8I GCNNGC 4 cut(s) 571, 610, 1526, 2853
CaiI CAGNNNCTG 2 cut(s) 620, 3278
CfoI GCGC 2 cut(s) 247, 327
Cfr10I RCCGGY 1 cut(s) 18
CseI GACGC 3 cut(s) 2079, 2277, 2504
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
DinI GGCGCC 1 cut(s) 326
DraIII CACNNNGTG 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 668
DriI GACNNNNNGTC 1 cut(s) 1233
DseDI GACNNNNNNGTC 1 cut(s) 668
Eam1104I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3082, 3240
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3082, 3240
EciI GGCGGA 2 cut(s) 288, 1568
Ecl136II GAGCTC 1 cut(s) 2773
Eco130I CCWWGG 3 cut(s) 765, 1871, 2924
Eco147I AGGCCT 1 cut(s) 3232
Eco24I GRGCYC 5 cut(s) 883, 1412, 2381, 2775, 2893
Eco31I GGTCTC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 9 cut(s) 34, 950, 1353, 1968, 2416, 2606, 2699, 2909, 3214
Eco53kI GAGCTC 1 cut(s) 2773
Eco57I CTGAAG 7 cut(s) 8, 1056, 1139, 1404, 1566, 1918, 2028
Eco72I CACGTG 1 cut(s) 2276
Eco88I CYCGRG 1 cut(s) 2374
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 2773
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 381, 1942
EcoRII CCWGG 3 cut(s) 385, 1627, 2719
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 3 cut(s) 765, 1871, 2924
EcoT22I ATGCAT 4 cut(s) 2404, 2539, 2674, 2836
EcoT38I GRGCYC 5 cut(s) 883, 1412, 2381, 2775, 2893
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 3 cut(s) 765, 1871, 2924
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 2 cut(s) 2450, 3270
FauI CCCGC 3 cut(s) 45, 647, 1625
FbaI TGATCA 1 cut(s) 1468
FblI GTMKAC 3 cut(s) 449, 1237, 2752
Fnu4HI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1650, 1653, 2499, 2792
FriOI GRGCYC 5 cut(s) 883, 1412, 2381, 2775, 2893
Fsp4HI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1650, 1653, 2499, 2792
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 2 cut(s) 246, 326
GluI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1650, 1653, 2499, 2792
GsaI CCCAGC 1 cut(s) 1686
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 5 cut(s) 9, 763, 3077, 3151, 3232
HapII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1658
HgaI GACGC 3 cut(s) 2079, 2277, 2504
HhaI GCGC 2 cut(s) 247, 327
Hin1I GRCGYC 1 cut(s) 325
Hin6I GCGC 2 cut(s) 245, 325
HinP1I GCGC 2 cut(s) 245, 325
HincII GTYRAC 2 cut(s) 450, 2983
HindII GTYRAC 2 cut(s) 450, 2983
HindIII AAGCTT 1 cut(s) 1604
HpaII CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1658
Hpy188III TCNNGA 7 cut(s) 434, 710, 1853, 2237, 2382, 2477, 2861
Hpy99I CGWCG 4 cut(s) 101, 424, 454, 1352
HpyCH4IV ACGT 3 cut(s) 1363, 2275, 2762
HpyF10VI GCNNNNNNNGC 8 cut(s) 322, 845, 1196, 1838, 2096, 2285, 2311, 2797
HpySE526I ACGT 3 cut(s) 1363, 2275, 2762
Hsp92I GRCGYC 1 cut(s) 325
HspAI GCGC 2 cut(s) 245, 325
KasI GGCGCC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 1468
LmnI GCTCC 4 cut(s) 436, 639, 2770, 3208
Lsp1109I GCAGC 7 cut(s) 249, 607, 832, 1177, 1661, 1664, 2510
LweI GCATC 9 cut(s) 853, 907, 1208, 1210, 2389, 2503, 2546, 2681, 2935
MaeII ACGT 3 cut(s) 1363, 2275, 2762
MaeIII GTNAC 3 cut(s) 88, 392, 1561
MfeI CAATTG 2 cut(s) 248, 926
MflI RGATCY 4 cut(s) 1450, 1615, 1635, 1898
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 4 cut(s) 68, 866, 2161, 2472
MmeI TCCRAC 4 cut(s) 610, 2615, 2979, 3201
Mph1103I ATGCAT 4 cut(s) 2404, 2539, 2674, 2836
MroI TCCGGA 1 cut(s) 433
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 2305
MspI CCGG 7 cut(s) 19, 41, 434, 1157, 1313, 1412, 1658
MspR9I CCNGG 6 cut(s) 387, 1158, 1412, 1629, 1659, 2721
MunI CAATTG 2 cut(s) 248, 926
Mva1269I GAATGC 2 cut(s) 556, 2674
MvaI CCWGG 3 cut(s) 387, 1629, 2721
MvnI CGCG 1 cut(s) 1854
MwoI GCNNNNNNNGC 8 cut(s) 322, 845, 1196, 1838, 2096, 2285, 2311, 2797
NarI GGCGCC 1 cut(s) 325
NciI CCSGG 3 cut(s) 1158, 1412, 1659
NcoI CCATGG 1 cut(s) 765
NlaIV GGNNCC 5 cut(s) 36, 326, 641, 1354, 2417
NmeAIII GCCGAG 3 cut(s) 301, 668, 1434
NmuCI GTSAC 2 cut(s) 88, 392
NruI TCGCGA 1 cut(s) 1854
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 4 cut(s) 2404, 2539, 2674, 2836
NspI RCATGY 2 cut(s) 374, 2332
NspV TTCGAA 1 cut(s) 1487
PaqCI CACCTGC 1 cut(s) 2185
PceI AGGCCT 1 cut(s) 3232
PcsI WCGNNNNNNNCGW 1 cut(s) 2272
PctI GAATGC 2 cut(s) 556, 2674
PfeI GAWTC 9 cut(s) 1330, 1538, 1556, 1765, 1952, 1979, 1988, 2875, 2954
PflFI GACNNNGTC 2 cut(s) 2017, 2462
PflMI CCANNNNNTGG 1 cut(s) 3278
PkrI GCNGC 9 cut(s) 264, 622, 762, 847, 1192, 1651, 1654, 2500, 2793
PleI GAGTC 4 cut(s) 67, 865, 2160, 2471
PluTI GGCGCC 1 cut(s) 328
PmaCI CACGTG 1 cut(s) 2276
PmlI CACGTG 1 cut(s) 2276
PpsI GAGTC 4 cut(s) 67, 865, 2160, 2471
Ppu21I YACGTR 1 cut(s) 2276
PshAI GACNNNNGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 2775
Psp1406I AACGTT 1 cut(s) 1363
Psp6I CCWGG 3 cut(s) 385, 1627, 2719
PspCI CACGTG 1 cut(s) 2276
PspEI GGTNACC 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 1682
PspGI CCWGG 3 cut(s) 385, 1627, 2719
PspN4I GGNNCC 5 cut(s) 36, 326, 641, 1354, 2417
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2208, 2240
PstI CTGCAG 3 cut(s) 1153, 1651, 2038
PstNI CAGNNNCTG 2 cut(s) 620, 3278
PsuI RGATCY 4 cut(s) 1450, 1615, 1635, 1898
PsyI GACNNNGTC 2 cut(s) 2017, 2462
PvuII CAGCTG 1 cut(s) 2305
RruI TCGCGA 1 cut(s) 1854
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 2775
SalI GTCGAC 1 cut(s) 448
SatI GCNGC 9 cut(s) 263, 621, 761, 846, 1191, 1650, 1653, 2499, 2792
SchI GAGTC 4 cut(s) 68, 866, 2161, 2472
ScrFI CCNGG 6 cut(s) 387, 1158, 1412, 1629, 1659, 2721
SfaNI GCATC 9 cut(s) 853, 907, 1208, 1210, 2389, 2503, 2546, 2681, 2935
SfcI CTRYAG 3 cut(s) 1149, 1647, 2034
SfoI GGCGCC 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 1487
SinI GGWCC 9 cut(s) 34, 950, 1353, 1968, 2416, 2606, 2699, 2909, 3214
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 910, 2193
SmoI CTYRAG 2 cut(s) 910, 2193
SpeI ACTAGT 1 cut(s) 2144
SseBI AGGCCT 1 cut(s) 3232
SspDI GGCGCC 1 cut(s) 324
SstI GAGCTC 1 cut(s) 2775
StuI AGGCCT 1 cut(s) 3232
StyD4I CCNGG 6 cut(s) 385, 1156, 1410, 1627, 1657, 2719
StyI CCWWGG 3 cut(s) 765, 1871, 2924
TaiI ACGT 3 cut(s) 1366, 2278, 2765
TaqI TCGA 7 cut(s) 96, 449, 998, 1487, 2878, 2893, 3058
TatI WGTACW 4 cut(s) 987, 1052, 1694, 2504
TauI GCSGC 2 cut(s) 763, 2794
TfiI GAWTC 9 cut(s) 1330, 1538, 1556, 1765, 1952, 1979, 1988, 2875, 2954
TscAI CASTG 9 cut(s) 550, 621, 903, 1158, 1312, 1340, 2608, 2801, 3279
TseFI GTSAC 2 cut(s) 88, 392
TseI GCWGC 7 cut(s) 262, 620, 845, 1190, 1649, 1652, 2498
Tsp45I GTSAC 2 cut(s) 88, 392
TspGWI ACGGA 2 cut(s) 1345, 3130
TspRI CASTG 9 cut(s) 550, 621, 903, 1158, 1312, 1340, 2608, 2801, 3279
Tth111I GACNNNGTC 2 cut(s) 2017, 2462
Van91I CCANNNNNTGG 1 cut(s) 3278
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 9 cut(s) 34, 950, 1353, 1968, 2416, 2606, 2699, 2909, 3214
XapI RAATTY 4 cut(s) 381, 932, 1270, 1942
XbaI TCTAGA 1 cut(s) 2381
XceI RCATGY 2 cut(s) 374, 2332
XmaJI CCTAGG 1 cut(s) 2924
XmiI GTMKAC 3 cut(s) 449, 1237, 2752
Zsp2I ATGCAT 4 cut(s) 2404, 2539, 2674, 2836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.