FvH4_5g10620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013452
Physical Location & Seq
Reverse (-)
6032150 .. 6037913
5764 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10620.t1

Sequence Viewer

Length: 3354 bp
ATGAAAGGACGATCCCACCGGTTTCAGAGTTCGGACCCGCCGGACGACTGGGTGGACGAGTCGTGGACGGTGGATTGTTTGTGTGGTGTGACTTTCGACGATGGGGAGGAGATGGTGAATTGCGACGAGTGCGGTGTTTGGGTGCACACGCGGTGTTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGATAAATGTAAGAGGAGGAATAGTAGGAATGATAGTGAGGAAACTGAGGTTGCGCAATTGTTGGTTGAGCTGCCGACTAAGACAGTGAGGATGGAGAGTTCGTTTCCGCCGCCGCCGAGTATGCCTGCTCGGCGGCCTCTTAGGCTTTGGACAGATATTCCGATGGAGGAGAGGGTGCATGTGCAGGGGATTCCGGGGGGTGATCCTGCTTTATTTGGTGGACTGTCGTCGGTTTTTACTCCGGAGCTGTGGAAGTCTACGGGATATGTGCCGAAAAAGTTTAATTTTCAGTACAGGGAGTTCCCTTGTTGGGACAAGAAAGAGGAGGCTGATGATAGATTTGATGAAGACAGTGAGAATGCTGTGGATAAAGGTGCCGGTGTTCTGTTCTCGTTGTTGAATGAGAGTGTCTTGGCAAACCCAGTGGCTGCTTTGGTTGGAATGAGGAGCCGAGAAGGTGGGTATGACAAGAGGGTGTCTTTGAAAGAGACAAAAAGGTGGGATAAGGAGGTTCGTGATCTTAGGTGTGCTCAGAGTGGTGGGAAGAAGGAGAGGAGTTTGCTTCGGCCCATGGTGTTACATACAGGAAAGCGTAAGAAGGACGATCTTGGGACATCCAAAGATCGGATTGCCAAGAAGAGGGCTAGAGCTGCTGAGAAAGAGGCTGATGCAAGGAAGAGAGGGGCTCAATCGTCTAAATCAGTGTTTACACCCTCAAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATTTCTAAGGCTGATGTTCAGAGTGTGAAGTATAAGAGGTCAAGCAACTCTGTGGTTCGAGAACCTGCAACAAATGTTTCTCTTGCGACAGACTATACTGTTGAAAAGCACTCTTCCGAAGCCTTACTTTCTGATAGGTCTAAAACAGTTGGTGATGGACTAAAGGAAGACAAGGTGGAACACCAAGTTTCTACAGTGCCGGGGAACATGACTATCACCAAAATGGATGATGCTGCTGTTGCATCATTGTTAGAATTAAATGATGCTAGTAGGACTGATTGTCTACAAGAACAGGGAGATAGCACAGAAGATGATAATGTGAATGTCAAACCACCCATCGAAAATGTTAGCACACCACCAGAGGTTGAAGATCAGAATCACTGTCCTACTGGCGACAGGTCCATTCAACGTTCTCCCAATGGAAAAACTGAAGACCATGAGGACATTTCTCGGAGCCTGTTGGATGTACAGTCTTCTCTTCACGGTGATGCAAAAGATCTTGGCAAATGTTCCGATCAAGTGTCTGAAAGTGCAAAAGATAATGCTGTAACATTAAACATCCCACTATCCAGTGACCAGAAGGTGCAAAGTGCCGAAAAAACTTCTGAAGCAGTTGATTCTCATACAGACAGGGGTGATGTAGTATCTGGTGATTGCCAGCCTAAACGGGAATCAGAAAGTTTGGCAGGTTCAATTACTCTGCAAAAATGTTCTTCAGATGTCAAACATGGTTCAAAGCTTTCAGAAGATCTTTCAAAGGCTGGTGGGATCTTAAACTCTGCAGCAACACCGGGTCAGCTTAAAACAACTTCAAGTGCTGGGAAATCTTCTACTGTTCCATGCACGAGTTTGACCCCCAAATCATCCACTCCTCAGAACTTAAAATCTGGGGATGTTCAGAATCCTAATCCATTTACTAAGCAACGAGTAGTGTCTGAAAGTAAAGTTAGCATCAAGAAAGATCGTGCTTCATCTGCAGACATGGATCATGATAAGGACAATATGCCAAGGAAAATAGTAAAAGAGCACTTAAGATCCCCTACAAGTTCTGCTCTGAAAACCCCACACTTTAGCAGGAATTCACATGATTCTGTATCCAAGCGGACCACATCAGAATCAAAAGATTCATTGCTTCACTCATCATCCAAGACATTGTCAGAAGGAGATACTGCAGTTCCTGCCGGCTCTAGTGAGAAGGTACATGGGCAGAATAAAAGTTCAGCTTCAAGTGCAATGCAAAGAGGTGAAAAGTTGAACCAGACAACTTCTTCCAAGACTAGTCAGAATCATGCACCACCTGCTTGTCCACCTGCACCATCTAGTTCTCAAGCAAAACTAAGTGACGAGGAACTCGCTTTACTTTTACATCAAGAACTCAACAGTTCTCCCAGAGTACCTCGTGTACCACGTGCTCGTCATGCGTCTAGCTTACCTCAGCTGGCTTCTCCAACTGCAGCAAGCATGTTAATAAAGCGTACATCTTCTTCTAGCGGAAAGGATCACAACGCGGGCTCTAGAAGAAAAGTGAGGGATGCTTATAAAGATGGGGTCCGAAGTTCCCGTGAACTGGATGATGAAGCTAAGAAGATGGACAGAGTCCCATCTTCTCCAGATCGTAGAAGACAAGACACAGCAAGTACAGTGGACGCTGCTGCTAAGAGGGAGGAGAATGCATCTTCAACAGCCTCACATTCTTACAAAAAGACTATCCCTTCTACCTCCATTCCAACTGCAAGCAGTGGTCGATCTTCATCCACTGAGGCTAATGATCGCAATTTGCCATCTGTACGCAGTTCACCGAGGAATGTGTCTGACGATGATATGGGGGCAGTTGGTCCTGTTCATCATACTTTACCAGGCTTAATAAATGAGATTATGAGTAAAGGCAGACGAATGACGTATGAGGAGCTCTGCAATGCTGTGATGCCGCACTGGCATAACCTGAGAAAGCACAATGGTGAGCGTTATGCATATACAAGTCCTTCGCAGGCCGTTCTTGATTGCCTTCGGAATCGACATGAATGGGCTCGATTAGTTGATCGTGGTCCGAAGACAAACCCTAGGAAAAAGCGAAGACCTGATGCTGACGATTCAGAGGATAATGAATATGGCAGGGTCAATCCAAAGGAGTTAGACAGTAAAAGCATTGATACACAGAGAGAGGACTTCCCGAAGGGCAAGAGAAAAGCTCGAAAACGCAGAAGACTGGCCCTTCATGGACGAGGAATAAAGGATGTCAGGGAGAAACGGAAAACAGATGTGCTCACCGACGATGATGTTGGGCCGTCGTTTTCTAATTCCACCGAGGAAACTGTGTCAGAGGATGATAATCAGGGAGGTGGAGCAGGTCCAGTTGGAAGTGAGGCCACTTCTAGTTCAGAGGAGGCAGGGACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1118

Amino Acids

122.43

Weight (kDa)

8.22

Isoelectric Point (pI)

57.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 935 - 997 4.8e-34 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2493
AarI CACCTGC 2 cut(s) 2260, 2272
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 5 cut(s) 1027, 1631, 2260, 2272, 3290
AccB1I GGYRCC 1 cut(s) 566
AccI GTMKAC 2 cut(s) 449, 1237
AccII CGCG 2 cut(s) 151, 2462
AccIII TCCGGA 1 cut(s) 433
AclI AACGTT 1 cut(s) 1363
AclWI GGATC 6 cut(s) 6, 389, 1730, 1947, 1983, 2460
AcsI RAATTY 2 cut(s) 929, 2032
AcuI CTGAAG 3 cut(s) 1404, 1581, 1653
AcvI CACGTG 1 cut(s) 2363
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 8 cut(s) 485, 1422, 2154, 2349, 2358, 2431, 2593, 2742
AfiI CCNNNNNNNGG 4 cut(s) 502, 816, 831, 2521
AflII CTTAAG 1 cut(s) 1984
AgeI ACCGGT 1 cut(s) 18
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 2231
AjnI CCWGG 1 cut(s) 2809
AleI CACNNNNGTG 1 cut(s) 2910
AloI GAACNNNNNNTCC 2 cut(s) 274, 306
Alw21I GWGCWC 6 cut(s) 147, 724, 1983, 2368, 2865, 3219
Alw26I GTCTC 1 cut(s) 674
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 6 cut(s) 6, 389, 1730, 1947, 1983, 2460
AlwNI CAGNNNCTG 2 cut(s) 620, 2132
Aor13HI TCCGGA 1 cut(s) 433
AoxI GGCC 6 cut(s) 326, 758, 2943, 3162, 3236, 3318
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 8 cut(s) 262, 620, 842, 1187, 1736, 2408, 2603, 2606
ApoI RAATTY 2 cut(s) 929, 2032
AsiGI ACCGGT 1 cut(s) 18
AspA2I CCTAGG 1 cut(s) 3014
AspLEI GCGC 1 cut(s) 247
AsuC2I CCSGG 3 cut(s) 387, 1155, 1746
AvaII GGWCC 8 cut(s) 34, 947, 1353, 2058, 2503, 2789, 2999, 3302
AvrII CCTAGG 1 cut(s) 3014
BaeGI GKGCMC 1 cut(s) 147
BaeI ACNNNNGTAYC 2 cut(s) 2112, 2145
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 4 cut(s) 880, 2468, 2865, 2983
BarI GAAGNNNNNNTAC 2 cut(s) 2422, 2454
BauI CACGAG 2 cut(s) 1798, 2352
BbrPI CACGTG 1 cut(s) 2363
BbsI GAAGAC 8 cut(s) 546, 1128, 1392, 1419, 2581, 3011, 3034, 3163
Bbv12I GWGCWC 6 cut(s) 147, 724, 1983, 2368, 2865, 3219
BbvCI CCTCAGC 1 cut(s) 2388
BbvI GCAGC 8 cut(s) 249, 607, 829, 1174, 1748, 2420, 2590, 2593
BceAI ACGGC 2 cut(s) 2930, 3223
BciT130I CCWGG 1 cut(s) 2811
BciVI GTATCC 1 cut(s) 2059
BcnI CCSGG 3 cut(s) 387, 1155, 1746
BcoDI GTCTC 1 cut(s) 674
BcuI ACTAGT 1 cut(s) 2231
BfmI CTRYAG 5 cut(s) 1146, 1734, 1929, 2124, 2406
BfrI CTTAAG 1 cut(s) 1984
BfuAI ACCTGC 5 cut(s) 1027, 1631, 2260, 2272, 3290
BfuI GTATCC 1 cut(s) 2059
BglI GCCNNNNNGGC 3 cut(s) 322, 334, 2887
BglII AGATCT 2 cut(s) 1450, 1702
BlnI CCTAGG 1 cut(s) 3014
Bme1390I CCNGG 4 cut(s) 387, 1155, 1746, 2811
Bme18I GGWCC 8 cut(s) 34, 947, 1353, 2058, 2503, 2789, 2999, 3302
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmiI GGNNCC 5 cut(s) 36, 568, 641, 1409, 2504
BmrFI CCNGG 4 cut(s) 387, 1155, 1746, 2811
BmrI ACTGGG 2 cut(s) 58, 608
BmuI ACTGGG 2 cut(s) 58, 608
BoxI GACNNNNGTC 2 cut(s) 418, 945
BpiI GAAGAC 8 cut(s) 546, 1128, 1392, 1419, 2581, 3011, 3034, 3163
BplI GAGNNNNNCTC 4 cut(s) 862, 894, 3127, 3159
BpmI CTGGAG 1 cut(s) 2547
Bpu10I CCTNAGC 1 cut(s) 2388
BpuEI CTTGAG 2 cut(s) 892, 2265
BpuMI CCSGG 3 cut(s) 387, 1155, 1746
BsaAI YACGTR 1 cut(s) 2363
BsaBI GATNNNNATC 3 cut(s) 1329, 2704, 3282
BsaJI CCNNGG 7 cut(s) 386, 762, 1154, 1961, 2753, 3014, 3258
BsaWI WCCGGW 2 cut(s) 18, 433
BsaXI ACNNNNNCTCC 4 cut(s) 199, 229, 278, 308
Bsc4I CCNNNNNNNGG 4 cut(s) 502, 816, 831, 2521
Bse118I RCCGGY 3 cut(s) 18, 569, 2135
Bse1I ACTGG 8 cut(s) 53, 614, 1348, 1524, 2526, 2891, 3165, 3305
Bse3DI GCAATG 3 cut(s) 2081, 2193, 2875
Bse8I GATNNNNATC 3 cut(s) 1329, 2704, 3282
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 2811
BseDI CCNNGG 7 cut(s) 386, 762, 1154, 1961, 2753, 3014, 3258
BseJI GATNNNNATC 3 cut(s) 1329, 2704, 3282
BseLI CCNNNNNNNGG 4 cut(s) 502, 816, 831, 2521
BseMI GCAATG 3 cut(s) 2081, 2193, 2875
BseMII CTCAG 8 cut(s) 228, 737, 837, 927, 1841, 2402, 2703, 2888
BseNI ACTGG 8 cut(s) 53, 614, 1348, 1524, 2526, 2891, 3165, 3305
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 8 cut(s) 249, 607, 829, 1174, 1748, 2420, 2590, 2593
BseYI CCCAGC 1 cut(s) 1772
BsgI GTGCAG 2 cut(s) 395, 2250
Bsh1236I CGCG 2 cut(s) 151, 2462
BshFI GGCC 6 cut(s) 328, 760, 2945, 3164, 3238, 3320
BshNI GGYRCC 1 cut(s) 566
BshTI ACCGGT 1 cut(s) 18
BsiHKAI GWGCWC 6 cut(s) 147, 724, 1983, 2368, 2865, 3219
BsiSI CCGG 8 cut(s) 19, 41, 386, 434, 570, 1154, 1745, 2136
BslFI GGGAC 3 cut(s) 518, 817, 2537
BslI CCNNNNNNNGG 4 cut(s) 502, 816, 831, 2521
BsmAI GTCTC 1 cut(s) 674
BsmFI GGGAC 3 cut(s) 518, 817, 2537
BsmI GAATGC 2 cut(s) 556, 2629
BsnI GGCC 6 cut(s) 328, 760, 2945, 3164, 3238, 3320
Bsp1286I GDGCHC 9 cut(s) 147, 724, 880, 1983, 2368, 2468, 2865, 2983, 3219
Bsp13I TCCGGA 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 1420
Bsp19I CCATGG 1 cut(s) 762
BspANI GGCC 6 cut(s) 328, 760, 2945, 3164, 3238, 3320
BspCNI CTCAG 8 cut(s) 229, 736, 838, 928, 1840, 2401, 2704, 2889
BspEI TCCGGA 1 cut(s) 433
BspFNI CGCG 2 cut(s) 151, 2462
BspHI TCATGA 1 cut(s) 1942
BspLI GGNNCC 5 cut(s) 36, 568, 641, 1409, 2504
BspMAI CTGCAG 4 cut(s) 1738, 1933, 2128, 2410
BspMI ACCTGC 5 cut(s) 1027, 1631, 2260, 2272, 3290
BspPI GGATC 6 cut(s) 6, 389, 1730, 1947, 1983, 2460
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 1984
BsrDI GCAATG 3 cut(s) 2081, 2193, 2875
BsrFI RCCGGY 3 cut(s) 18, 569, 2135
BsrGI TGTACA 1 cut(s) 1420
BsrI ACTGG 8 cut(s) 53, 614, 1348, 1524, 2526, 2891, 3165, 3305
BssAI RCCGGY 3 cut(s) 18, 569, 2135
BssECI CCNNGG 7 cut(s) 386, 762, 1154, 1961, 2753, 3014, 3258
BssSI CACGAG 2 cut(s) 1798, 2352
BssT1I CCWWGG 3 cut(s) 762, 1961, 3014
Bst2BI CACGAG 2 cut(s) 1798, 2352
Bst2UI CCWGG 1 cut(s) 2811
Bst6I CTCTTC 4 cut(s) 824, 863, 1072, 1437
BstAFI CTTAAG 1 cut(s) 1984
BstAPI GCANNNNNTGC 2 cut(s) 2132, 2252
BstAUI TGTACA 1 cut(s) 1420
BstBAI YACGTR 1 cut(s) 2363
BstC8I GCNNGC 8 cut(s) 318, 1613, 2137, 2394, 2413, 2464, 2689, 2943
BstDSI CCRYGG 1 cut(s) 762
BstFNI CGCG 2 cut(s) 151, 2462
BstHHI GCGC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 674
BstNI CCWGG 1 cut(s) 2811
BstNSI RCATGY 2 cut(s) 374, 2419
BstPAI GACNNNNGTC 2 cut(s) 418, 945
BstSCI CCNGG 4 cut(s) 385, 1153, 1744, 2809
BstSFI CTRYAG 5 cut(s) 1146, 1734, 1929, 2124, 2406
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 2 cut(s) 151, 2462
BstV1I GCAGC 8 cut(s) 249, 607, 829, 1174, 1748, 2420, 2590, 2593
BstV2I GAAGAC 8 cut(s) 546, 1128, 1392, 1419, 2581, 3011, 3034, 3163
BstX2I RGATCY 4 cut(s) 1450, 1702, 1722, 1988
BstYI RGATCY 4 cut(s) 1450, 1702, 1722, 1988
BsuI GTATCC 1 cut(s) 2059
BsuRI GGCC 6 cut(s) 328, 760, 2945, 3164, 3238, 3320
BtgI CCRYGG 1 cut(s) 762
BtsI GCAGTG 1 cut(s) 2698
BveI ACCTGC 5 cut(s) 1027, 1631, 2260, 2272, 3290
Cac8I GCNNGC 8 cut(s) 318, 1613, 2137, 2394, 2413, 2464, 2689, 2943
CaiI CAGNNNCTG 2 cut(s) 620, 2132
CciI TCATGA 1 cut(s) 1942
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 3 cut(s) 18, 569, 2135
CseI GACGC 2 cut(s) 2364, 2609
Csp6I GTAC 8 cut(s) 484, 1421, 2153, 2348, 2357, 2430, 2592, 2741
CspAI ACCGGT 1 cut(s) 18
CspCI CAANNNNNGTGG 4 cut(s) 597, 632, 2577, 2612
CviQI GTAC 8 cut(s) 484, 1421, 2153, 2348, 2357, 2430, 2592, 2741
DraIII CACNNNGTG 1 cut(s) 153
DriI GACNNNNNGTC 1 cut(s) 1233
Eam1104I CTCTTC 4 cut(s) 824, 863, 1072, 1437
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 4 cut(s) 824, 863, 1072, 1437
EciI GGCGGA 1 cut(s) 288
Ecl136II GAGCTC 1 cut(s) 2863
Eco130I CCWWGG 3 cut(s) 762, 1961, 3014
Eco24I GRGCYC 4 cut(s) 880, 2468, 2865, 2983
Eco47I GGWCC 8 cut(s) 34, 947, 1353, 2058, 2503, 2789, 2999, 3302
Eco53kI GAGCTC 1 cut(s) 2863
Eco57I CTGAAG 3 cut(s) 1404, 1581, 1653
Eco72I CACGTG 1 cut(s) 2363
EcoICRI GAGCTC 1 cut(s) 2863
EcoRI GAATTC 1 cut(s) 2032
EcoRII CCWGG 1 cut(s) 2809
EcoT14I CCWWGG 3 cut(s) 762, 1961, 3014
EcoT22I ATGCAT 2 cut(s) 2629, 2926
EcoT38I GRGCYC 4 cut(s) 880, 2468, 2865, 2983
ErhI CCWWGG 3 cut(s) 762, 1961, 3014
FalI AAGNNNNNCTT 4 cut(s) 1065, 1097, 2161, 2193
FaqI GGGAC 3 cut(s) 518, 817, 2537
FauI CCCGC 2 cut(s) 45, 2455
FblI GTMKAC 2 cut(s) 449, 1237
FriOI GRGCYC 4 cut(s) 880, 2468, 2865, 2983
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 1776
GsuI CTGGAG 1 cut(s) 2547
HaeIII GGCC 6 cut(s) 328, 760, 2945, 3164, 3238, 3320
HapII CCGG 8 cut(s) 19, 41, 386, 434, 570, 1154, 1745, 2136
HgaI GACGC 2 cut(s) 2364, 2609
HhaI GCGC 1 cut(s) 247
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 1691
HpaII CCGG 8 cut(s) 19, 41, 386, 434, 570, 1154, 1745, 2136
Hpy99I CGWCG 5 cut(s) 101, 128, 424, 3227, 3244
HpyCH4IV ACGT 3 cut(s) 1363, 2362, 2852
HpySE526I ACGT 3 cut(s) 1363, 2362, 2852
HspAI GCGC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 433
KroI GCCGGC 1 cut(s) 2135
KroNI GCCGGC 1 cut(s) 2137
LmnI GCTCC 5 cut(s) 436, 639, 1407, 2860, 3296
Lsp1109I GCAGC 8 cut(s) 249, 607, 829, 1174, 1748, 2420, 2590, 2593
MaeII ACGT 3 cut(s) 1363, 2362, 2852
MaeIII GTNAC 5 cut(s) 88, 768, 1501, 1526, 2294
MfeI CAATTG 2 cut(s) 248, 923
MflI RGATCY 4 cut(s) 1450, 1702, 1722, 1988
MhlI GDGCHC 9 cut(s) 147, 724, 880, 1983, 2368, 2468, 2865, 2983, 3219
MlyI GAGTC 2 cut(s) 68, 2559
MmeI TCCRAC 5 cut(s) 610, 1395, 2426, 2705, 3289
Mph1103I ATGCAT 2 cut(s) 2629, 2926
MroI TCCGGA 1 cut(s) 433
MroNI GCCGGC 1 cut(s) 2135
MseI TTAA 9 cut(s) 474, 1211, 1508, 1727, 1755, 1835, 1985, 2420, 2816
MslI CAYNNNNRTG 3 cut(s) 1175, 1440, 2910
MspA1I CMGCKG 1 cut(s) 2392
MspCI CTTAAG 1 cut(s) 1984
MspI CCGG 8 cut(s) 19, 41, 386, 434, 570, 1154, 1745, 2136
MspR9I CCNGG 4 cut(s) 387, 1155, 1746, 2811
MunI CAATTG 2 cut(s) 248, 923
Mva1269I GAATGC 2 cut(s) 556, 2629
MvaI CCWGG 1 cut(s) 2811
MvnI CGCG 2 cut(s) 151, 2462
NaeI GCCGGC 1 cut(s) 2137
NciI CCSGG 3 cut(s) 387, 1155, 1746
NcoI CCATGG 1 cut(s) 762
NgoMIV GCCGGC 1 cut(s) 2135
NlaIV GGNNCC 5 cut(s) 36, 568, 641, 1409, 2504
NmeAIII GCCGAG 3 cut(s) 301, 333, 668
NmuCI GTSAC 3 cut(s) 88, 1526, 2294
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 2 cut(s) 2629, 2926
NspI RCATGY 2 cut(s) 374, 2419
OliI CACNNNNGTG 1 cut(s) 2910
PagI TCATGA 1 cut(s) 1942
PaqCI CACCTGC 2 cut(s) 2260, 2272
PcsI WCGNNNNNNNCGW 1 cut(s) 2359
PctI GAATGC 2 cut(s) 556, 2629
PdiI GCCGGC 1 cut(s) 2137
PflFI GACNNNGTC 2 cut(s) 2107, 2549
PinAI ACCGGT 1 cut(s) 18
PleI GAGTC 2 cut(s) 67, 2558
PmaCI CACGTG 1 cut(s) 2363
PmlI CACGTG 1 cut(s) 2363
PpsI GAGTC 2 cut(s) 67, 2558
Ppu21I YACGTR 1 cut(s) 2363
PshAI GACNNNNGTC 2 cut(s) 418, 945
PsiI TTATAA 1 cut(s) 2493
Psp124BI GAGCTC 1 cut(s) 2865
Psp1406I AACGTT 1 cut(s) 1363
Psp6I CCWGG 1 cut(s) 2809
PspCI CACGTG 1 cut(s) 2363
PspFI CCCAGC 1 cut(s) 1772
PspGI CCWGG 1 cut(s) 2809
PspN4I GGNNCC 5 cut(s) 36, 568, 641, 1409, 2504
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2295, 2327
PstI CTGCAG 4 cut(s) 1738, 1933, 2128, 2410
PstNI CAGNNNCTG 2 cut(s) 620, 2132
PsuI RGATCY 4 cut(s) 1450, 1702, 1722, 1988
PsyI GACNNNGTC 2 cut(s) 2107, 2549
PvuII CAGCTG 1 cut(s) 2392
RsaI GTAC 8 cut(s) 485, 1422, 2154, 2349, 2358, 2431, 2593, 2742
RsaNI GTAC 8 cut(s) 484, 1421, 2153, 2348, 2357, 2430, 2592, 2741
RseI CAYNNNNRTG 3 cut(s) 1175, 1440, 2910
SacI GAGCTC 1 cut(s) 2865
SaqAI TTAA 9 cut(s) 474, 1211, 1508, 1727, 1755, 1835, 1985, 2420, 2816
SchI GAGTC 2 cut(s) 68, 2559
ScrFI CCNGG 4 cut(s) 387, 1155, 1746, 2811
SduI GDGCHC 9 cut(s) 147, 724, 880, 1983, 2368, 2468, 2865, 2983, 3219
SfcI CTRYAG 5 cut(s) 1146, 1734, 1929, 2124, 2406
SinI GGWCC 8 cut(s) 34, 947, 1353, 2058, 2503, 2789, 2999, 3302
SmiMI CAYNNNNRTG 3 cut(s) 1175, 1440, 2910
SmlI CTYRAG 3 cut(s) 907, 1984, 2280
SmoI CTYRAG 3 cut(s) 907, 1984, 2280
SpeI ACTAGT 1 cut(s) 2231
SstI GAGCTC 1 cut(s) 2865
StyD4I CCNGG 4 cut(s) 385, 1153, 1744, 2809
StyI CCWWGG 3 cut(s) 762, 1961, 3014
TaiI ACGT 3 cut(s) 1366, 2365, 2855
TaqI TCGA 7 cut(s) 96, 1012, 1293, 2698, 2968, 2983, 3145
TatI WGTACW 3 cut(s) 483, 1420, 2591
TauI GCSGC 4 cut(s) 304, 307, 328, 2884
Tru1I TTAA 9 cut(s) 474, 1211, 1508, 1727, 1755, 1835, 1985, 2420, 2816
Tru9I TTAA 9 cut(s) 474, 1211, 1508, 1727, 1755, 1835, 1985, 2420, 2816
TseFI GTSAC 3 cut(s) 88, 1526, 2294
TseI GCWGC 8 cut(s) 262, 620, 842, 1187, 1736, 2408, 2603, 2606
Tsp45I GTSAC 3 cut(s) 88, 1526, 2294
TspGWI ACGGA 1 cut(s) 3217
Tth111I GACNNNGTC 2 cut(s) 2107, 2549
Vha464I CTTAAG 1 cut(s) 1984
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 8 cut(s) 34, 947, 1353, 2058, 2503, 2789, 2999, 3302
XapI RAATTY 2 cut(s) 929, 2032
XbaI TCTAGA 1 cut(s) 2468
XceI RCATGY 2 cut(s) 374, 2419
XmaJI CCTAGG 1 cut(s) 3014
XmiI GTMKAC 2 cut(s) 449, 1237
Zsp2I ATGCAT 2 cut(s) 2629, 2926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.