RLG00000004774

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013452
Physical Location & Seq
Reverse (-)
62158930 .. 62163961
5032 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004774

Sequence Viewer

Length: 3357 bp
ATGAAAGGCCGATCTCACCGGCTTCAGAGTTCGGACCCGCCGGACGACTGGGTGGATGAGTCGTGGACGGTGGATTGTTTGTGTGGTGTGACTTTCGACGATGGGGAAGAGATGGTGAATTGTGATGAGTGCGGTGTTTGGGTGCACACTCGGTGTTCTAGGTATGTGAAGGGAGATGATAATTTTGTTTGTGATAAATGTAAGAGCAGGAATAGTAGGAATGATAGTGAAGAAACTGAGGTTGCGCAATTGTTGGTTGAGCTGCCTACCAAGACTGTGAGAATGGAGAGTTCATTTCCGCCACCACCGAATATGCCTACTCGGCGCCCTCTTAGGCTTTGGACTGATATACCAATGGAGGAGAGGGTGCATGTGCAGGGAATTCCAGGGGGTGACCCTGCTATATTTGGTGGGTTGTCGTCGGTTTTTACTCCAGAGCTGTGGAAGTCGACAGGATATGTGCCCAAAAAGTTTAATTTTCAGTATAGGGAGTTCCCTTGTTGGGATAAGAAGGAGGAGGCTGATGTTAGATTTGATGAAGACAGTGAGAATGCTGTGGATAAAGGTGCCGGCGTTCTGTTCTCTTTGTTGAATGAGAGTGTCTTGGCAAGCCCAGTGGCTGCTTTGGTTGGAATGAGGAGCCGAGAAGAAGGCGGGTATGACAAGAAGGTCTCTTTGAAAGAGACAAAAAGGTGGGATAAGGAGGTTCGTGATCTTAGATGTGCTCAGAGTGGTGTGAAGAAGGAGAGGAGTTTACTGCGGCCCATGGTATTACATACGGGTAAGCGTAAGAAGGACGATCTGGGTACCTCCAAAGATCGGAGTGCCAAGAAGAGGGCTAGAGCTGCTGAGAAAGAGGCTGATGCAAAGAAGAGAGGGGCTCAATCTTCTAAATCAGTGTTTACACCCTCAAGTGATGCAAAACAATTGGAATTTTCTGAGGACAGAGGTCCGAAGATTTCTAAGGCTGATGTTCAGAGTACGAAGTACAAGAAGTCGAGCAACTCTGTGGTTAGAGAACCTGCACCAAATGTTTCTCTTGCGACAGACTGTACTGTTGAAAATCACTCTTCAGAATCCTTACTTTCTGATAGGTCTAAAAAGATTGATGATGGGCTGAAGGAAGACAAGGTGGAACACCAAGTTTCTGCAGTGCCGGGGCACATGACTATCACCAAAATGGATGGTGCTGCTGTTGCATCATTGTTAGAATTAAATGATGCTAGGACTGATTGTCTACAAGAACAGGGAGATAGCACAGAAGATGAAAATTTGAATGTGAAACCACCCATTGAAAATGTTAGCACTGCACCGGAGGTTGAAGTTCAGAATCACTGTCCTACTAGCGACGGGTCCGTACAACGTTCTCCCAATAAGAAAACTGAAGACCATGAGGACAATTCTCAGAGCCCGGTGCATGTGCAGTCTTCTCTTCATGGTGAGGCAAAAGATCTCGGCAAATCTTCTGATCAAGTGTCTGAAAGTTCGAAACTTAATGGTGTAATAGTAAACATCCCACTGTCCAGTGACCATAAGGTGCAAGGTGCTGACAGAACTTCTGAAGCAGTTAGTGATTCTCATACAGAGAGGGGTGATGAATTATCTGGCGATTGCCAGCCTAAACGGGAATCAGAAGATTTGGCGGATTCAGTTACTCTGCAAAAATGTTCTTCAGATGTCAAACATGGTTTGAAGCTTTCAGAAGATCTTTCAAAACCAGGCGCGATCTTAAACTCTGCAGCAGCACCGGGTCAGCTTAAAACAGCAAGTGTTGGGAAATCATGTACTGTTCCATCCACCAGTTTGACCGCCAAATCATCCACTCCTCAGAACTTAAAATCTGGTGATGTTCAGAATCCTAATCCATTTACTAAGCAGCGAGTAATGTCTGAAAGTAAAGTTAGCATTAAGAAAGATCGTGCTTCATCTGCGGACATGGATCACGATAAGGACAATATGCCAAGGAAAATAGTAAAAGAGCAGTTAAGATCCCCTACAAGTTCTTCTCTGAAAACCTCACACTTCAGCAGGAATTCACATGATTCTGTATCCAAGCGGACCACATCAGAATCAAAAGATTCATTGCTTCACTCATCTTCCAAGACATTGTCAGAAGGGGATACTGCAGTTCCTTCAGGCTCTAGTGAGAAGGTTTTACATGGGCAGAACAAAAGTTCAGCGTCAAGTGCATTGCAAAGAGGTGAAAAGTTAAACCAGACAACTTCTTCTAAGACTAGTCAGAGTCATGCACCACCTTCTTGTCCACCTGCACCATCAAGTTCTCAAGCAAAACTAAGTGATGAGGAACTCGCTTTACTTTTACATCAAGAACTCAACAGTTCTCCCAGAGTACCTCGTGTACCACGTGCTCGTCATGCGTCTAGCTTACCTCAGCTGGCTTCTCCAACTGCAACAAGCATGTTAATAAAGCGTACATCTTCTTCTAGCGGAAAGGATCACAACTCGGGCTCTAGAAGAAAAGTGAGGGATGCATATAAAGATGGGGTCCGAAGTTCCCGTGAACTGGATGATGAAACTAAGAGGATGGACAGAGTTCCATCTTCTCCTGATCCTAGAAGGCAAGACGCAGCTAGTACAGTAGATGCTACTAGGAGGGAGGAAAATGCATCTTCAACAGCCTCACATTCTTACAAAAAGACCATCCCTTCTACCTCCATTCCAACTGCAAACAGTGGTCCATCTTCATCCACTGAGGCTAATGATCGCAATGTGTCATCAGTACGCAGTTCACCGAGGAATGCGTCTGACGATGATACAGGGGCAGTTGGTCCTGTTCATCCTACTTTACCAGGCTTGATAAATGAGATTATGAGTAAAGGTAGACGAATGACGTATGAGGAGCTCTGCAATGCTGTGATGCCGCACTGGCATAATCTGAGAAAGCACAATGGAGAGCGTTATGCATATACAAGTCCTTCGCAGGCTGTTCTTGATTGCCTTCGGAATCGACATGAATGGGCTCGATTAGTTGATCGTGGTCCGAAGACAAACCCTAGGAAAAAGCGAAAACTTGATGCTGATGATTCAGAGGATAATGAATATGGCAGGGTCAACAATCCAAAGGAGTTGGAAGGTAAAAGCATAGAGACACAGAGAGAGGACTATCCAAAGGGAAAGAGAAAAGCTCGAAAACGCAGAAGACTGGCCCTTCAAGGAAGAGGAATAAAGGATGTGAGGGAGAAACGGAAGGCAGATTTGCTCACCGATGATGATGTAGGGCCATCATTTTCTAATTCCACAGAGGAAACCGTGTCTGAGGATGATATTCAAGGAGGTGGAGCAGGTCCAGTTGGAAGTGAGGCCTCTTCTAGTTCAGAGGAGGAAGGGACGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

1119

Amino Acids

122.47

Weight (kDa)

7.35

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7648 PF24659 935 - 997 4.8e-34 Domain of unknown function (DUF7648)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013452)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32810
fragaria_vesca FvH4_5g10620
malus_domestica MD06G1121800.v1.1
prunus_persica Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1 Prupe.5G132500_v2.0.a1
pyrus_communis pycom06g11290
rosa_chinensis RchiOBHm_Chr7g0187521
rosa_laevigata RLG00000004774
rosa_multiflora Rmu_sc0019127.1_g000003
rosa_roxburghii Rroxscaffold_3G00267360
rosa_rugosa Rorug06G0482200 Rorug06G0482300 Rorug06G0482400
rosa_samantha Rh7AG087000 Rh7BG088400 Rh7CG087700 Rh7DG089300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2275
AasI GACNNNNNNGTC 1 cut(s) 668
Acc16I TGCGCA 1 cut(s) 246
Acc36I ACCTGC 3 cut(s) 1030, 2275, 3293
Acc65I GGTACC 1 cut(s) 806
AccB1I GGYRCC 3 cut(s) 324, 566, 806
AccI GTMKAC 3 cut(s) 449, 1237, 2842
AccII CGCG 1 cut(s) 1724
AclI AACGTT 1 cut(s) 1363
AclWI GGATC 4 cut(s) 1947, 1983, 2463, 2564
AcsI RAATTY 4 cut(s) 381, 932, 1270, 2032
AcuI CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2118
AcvI CACGTG 1 cut(s) 2366
AcyI GRCGYC 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 153
AfiI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 2234
AjnI CCWGG 3 cut(s) 385, 1717, 2809
Alw21I GWGCWC 4 cut(s) 147, 727, 2371, 2865
Alw26I GTCTC 3 cut(s) 676, 677, 3101
Alw44I GTGCAC 1 cut(s) 143
AlwI GGATC 4 cut(s) 1947, 1983, 2463, 2564
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 2464
AoxI GGCC 5 cut(s) 7, 761, 3165, 3239, 3321
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 8 cut(s) 262, 620, 845, 1190, 1739, 1742, 1876, 2588
ApoI RAATTY 4 cut(s) 381, 932, 1270, 2032
Asp718I GGTACC 1 cut(s) 806
AspA2I CCTAGG 1 cut(s) 3014
AspLEI GCGC 3 cut(s) 247, 327, 1724
AsuC2I CCSGG 3 cut(s) 1158, 1412, 1749
AsuII TTCGAA 1 cut(s) 1487
AvaI CYCGRG 1 cut(s) 2464
AvaII GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3305
AvrII CCTAGG 1 cut(s) 3014
BaeGI GKGCMC 3 cut(s) 147, 465, 1164
BaeI ACNNNNGTAYC 2 cut(s) 2112, 2145
BanI GGYRCC 3 cut(s) 324, 566, 806
BanII GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
BarI GAAGNNNNNNTAC 2 cut(s) 2425, 2457
BauI CACGAG 1 cut(s) 2355
BbrPI CACGTG 1 cut(s) 2366
BbsI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 3011, 3166
Bbv12I GWGCWC 4 cut(s) 147, 727, 2371, 2865
BbvCI CCTCAGC 1 cut(s) 2391
BbvI GCAGC 8 cut(s) 249, 607, 832, 1177, 1751, 1754, 1888, 2600
BciT130I CCWGG 3 cut(s) 387, 1719, 2811
BciVI GTATCC 2 cut(s) 2059, 2113
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 3 cut(s) 1158, 1412, 1749
BcoDI GTCTC 3 cut(s) 676, 677, 3101
BcuI ACTAGT 1 cut(s) 2234
BfmI CTRYAG 3 cut(s) 1149, 1737, 2124
BfoI RGCGCY 1 cut(s) 328
BfuAI ACCTGC 3 cut(s) 1030, 2275, 3293
BfuI GTATCC 2 cut(s) 2059, 2113
BglI GCCNNNNNGGC 2 cut(s) 322, 2887
BglII AGATCT 2 cut(s) 1450, 1705
BlnI CCTAGG 1 cut(s) 3014
Bme1390I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1749, 2811
Bme18I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3305
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 1 cut(s) 2464
BmiI GGNNCC 7 cut(s) 36, 326, 568, 641, 808, 1354, 2507
BmrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1749, 2811
BmrI ACTGGG 2 cut(s) 58, 608
BmsI GCATC 9 cut(s) 853, 907, 1208, 1210, 2479, 2593, 2636, 2868, 3025
BmuI ACTGGG 2 cut(s) 58, 608
BoxI GACNNNNGTC 1 cut(s) 948
BpiI GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 3011, 3166
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 3130, 3162
BpmI CTGGAG 1 cut(s) 417
Bpu10I CCTNAGC 1 cut(s) 2391
Bpu14I TTCGAA 1 cut(s) 1487
BpuEI CTTGAG 2 cut(s) 895, 2268
BpuMI CCSGG 3 cut(s) 1158, 1412, 1749
BsaAI YACGTR 1 cut(s) 2366
BsaHI GRCGYC 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 6 cut(s) 386, 765, 1157, 1961, 2753, 3014
BsaWI WCCGGW 1 cut(s) 1312
Bsc4I CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
Bse118I RCCGGY 2 cut(s) 18, 569
Bse1I ACTGG 8 cut(s) 53, 614, 1524, 1800, 2529, 2891, 3168, 3308
Bse3DI GCAATG 4 cut(s) 2081, 2189, 2734, 2875
BseBI CCWGG 3 cut(s) 387, 1719, 2811
BseDI CCNNGG 6 cut(s) 386, 765, 1157, 1961, 2753, 3014
BseLI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
BseMI GCAATG 4 cut(s) 2081, 2189, 2734, 2875
BseNI ACTGG 8 cut(s) 53, 614, 1524, 1800, 2529, 2891, 3168, 3308
BseRI GAGGAG 7 cut(s) 374, 530, 652, 763, 1815, 2873, 3353
BseSI GKGCMC 3 cut(s) 147, 465, 1164
BseXI GCAGC 8 cut(s) 249, 607, 832, 1177, 1751, 1754, 1888, 2600
BsgI GTGCAG 5 cut(s) 395, 1008, 1293, 1442, 2253
Bsh1236I CGCG 1 cut(s) 1724
BshFI GGCC 5 cut(s) 9, 763, 3167, 3241, 3323
BshNI GGYRCC 3 cut(s) 324, 566, 806
BsiHKAI GWGCWC 4 cut(s) 147, 727, 2371, 2865
BsiHKCI CYCGRG 1 cut(s) 2464
BsiSI CCGG 7 cut(s) 19, 41, 570, 1157, 1313, 1412, 1748
BslI CCNNNNNNNGG 4 cut(s) 502, 819, 834, 2524
BsmAI GTCTC 3 cut(s) 676, 677, 3101
BsmI GAATGC 2 cut(s) 556, 2764
BsnI GGCC 5 cut(s) 9, 763, 3167, 3241, 3323
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 2464
Bsp119I TTCGAA 1 cut(s) 1487
Bsp19I CCATGG 1 cut(s) 765
BspANI GGCC 5 cut(s) 9, 763, 3167, 3241, 3323
BspFNI CGCG 1 cut(s) 1724
BspLI GGNNCC 7 cut(s) 36, 326, 568, 641, 808, 1354, 2507
BspMAI CTGCAG 3 cut(s) 1153, 1741, 2128
BspMI ACCTGC 3 cut(s) 1030, 2275, 3293
BspPI GGATC 4 cut(s) 1947, 1983, 2463, 2564
BspT104I TTCGAA 1 cut(s) 1487
BspT107I GGYRCC 3 cut(s) 324, 566, 806
BspTNI GGTCTC 1 cut(s) 676
BsrDI GCAATG 4 cut(s) 2081, 2189, 2734, 2875
BsrFI RCCGGY 2 cut(s) 18, 569
BsrI ACTGG 8 cut(s) 53, 614, 1524, 1800, 2529, 2891, 3168, 3308
BssAI RCCGGY 2 cut(s) 18, 569
BssECI CCNNGG 6 cut(s) 386, 765, 1157, 1961, 2753, 3014
BssNI GRCGYC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 2355
BssT1I CCWWGG 3 cut(s) 765, 1961, 3014
Bst2BI CACGAG 1 cut(s) 2355
Bst2UI CCWGG 3 cut(s) 387, 1719, 2811
Bst6I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3331
BstACI GRCGYC 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 2366
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 5 cut(s) 571, 610, 1616, 2397, 2943
BstDSI CCRYGG 1 cut(s) 765
BstEII GGTNACC 1 cut(s) 392
BstFNI CGCG 1 cut(s) 1724
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 3 cut(s) 247, 327, 1724
BstMAI GTCTC 3 cut(s) 676, 677, 3101
BstMWI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
BstNI CCWGG 3 cut(s) 387, 1719, 2811
BstNSI RCATGY 3 cut(s) 374, 1421, 2422
BstPAI GACNNNNGTC 1 cut(s) 948
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1747, 2809
BstSFI CTRYAG 3 cut(s) 1149, 1737, 2124
BstSLI GKGCMC 3 cut(s) 147, 465, 1164
BstUI CGCG 1 cut(s) 1724
BstV1I GCAGC 8 cut(s) 249, 607, 832, 1177, 1751, 1754, 1888, 2600
BstV2I GAAGAC 6 cut(s) 546, 1131, 1392, 1419, 3011, 3166
BstX2I RGATCY 3 cut(s) 1450, 1705, 1988
BstXI CCANNNNNNTGG 1 cut(s) 441
BstYI RGATCY 3 cut(s) 1450, 1705, 1988
BsuI GTATCC 2 cut(s) 2059, 2113
BsuRI GGCC 5 cut(s) 9, 763, 3167, 3241, 3323
BtgI CCRYGG 1 cut(s) 765
BtsI GCAGTG 2 cut(s) 1158, 1305
BveI ACCTGC 3 cut(s) 1030, 2275, 3293
Cac8I GCNNGC 5 cut(s) 571, 610, 1616, 2397, 2943
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 3 cut(s) 247, 327, 1724
Cfr10I RCCGGY 2 cut(s) 18, 569
CseI GACGC 4 cut(s) 2169, 2367, 2594, 2751
CspCI CAANNNNNGTGG 2 cut(s) 597, 632
DinI GGCGCC 1 cut(s) 326
DraIII CACNNNGTG 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 668
DriI GACNNNNNGTC 1 cut(s) 1233
DseDI GACNNNNNNGTC 1 cut(s) 668
Eam1104I CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3331
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 7 cut(s) 102, 827, 866, 1075, 1437, 3172, 3331
EciI GGCGGA 2 cut(s) 288, 1658
Ecl136II GAGCTC 1 cut(s) 2863
Eco130I CCWWGG 3 cut(s) 765, 1961, 3014
Eco147I AGGCCT 1 cut(s) 3323
Eco24I GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
Eco31I GGTCTC 1 cut(s) 676
Eco47I GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3305
Eco53kI GAGCTC 1 cut(s) 2863
Eco57I CTGAAG 8 cut(s) 8, 1056, 1139, 1404, 1581, 1656, 2008, 2118
Eco72I CACGTG 1 cut(s) 2366
Eco88I CYCGRG 1 cut(s) 2464
Eco91I GGTNACC 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 2863
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 381, 2032
EcoRII CCWGG 3 cut(s) 385, 1717, 2809
EcoT14I CCWWGG 3 cut(s) 765, 1961, 3014
EcoT22I ATGCAT 3 cut(s) 2494, 2629, 2926
EcoT38I GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 3 cut(s) 765, 1961, 3014
FauI CCCGC 2 cut(s) 45, 647
FbaI TGATCA 1 cut(s) 1468
FblI GTMKAC 3 cut(s) 449, 1237, 2842
FriOI GRGCYC 5 cut(s) 883, 1412, 2471, 2865, 2983
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 3 cut(s) 246, 326, 1723
GsuI CTGGAG 1 cut(s) 417
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 5 cut(s) 9, 763, 3167, 3241, 3323
HapII CCGG 7 cut(s) 19, 41, 570, 1157, 1313, 1412, 1748
HgaI GACGC 4 cut(s) 2169, 2367, 2594, 2751
HhaI GCGC 3 cut(s) 247, 327, 1724
Hin1I GRCGYC 1 cut(s) 325
Hin6I GCGC 3 cut(s) 245, 325, 1722
HinP1I GCGC 3 cut(s) 245, 325, 1722
HincII GTYRAC 2 cut(s) 450, 3073
HindII GTYRAC 2 cut(s) 450, 3073
HindIII AAGCTT 1 cut(s) 1694
HpaII CCGG 7 cut(s) 19, 41, 570, 1157, 1313, 1412, 1748
Hpy188III TCNNGA 7 cut(s) 434, 710, 1943, 2327, 2472, 2567, 2951
Hpy99I CGWCG 3 cut(s) 101, 424, 1352
HpyCH4IV ACGT 3 cut(s) 1363, 2365, 2852
HpyF10VI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
HpySE526I ACGT 3 cut(s) 1363, 2365, 2852
Hsp92I GRCGYC 1 cut(s) 325
HspAI GCGC 3 cut(s) 245, 325, 1722
KasI GGCGCC 1 cut(s) 324
KpnI GGTACC 1 cut(s) 810
KroI GCCGGC 1 cut(s) 569
KroNI GCCGGC 1 cut(s) 571
Ksp22I TGATCA 1 cut(s) 1468
LmnI GCTCC 3 cut(s) 639, 2860, 3299
Lsp1109I GCAGC 8 cut(s) 249, 607, 832, 1177, 1751, 1754, 1888, 2600
LweI GCATC 9 cut(s) 853, 907, 1208, 1210, 2479, 2593, 2636, 2868, 3025
MaeII ACGT 3 cut(s) 1363, 2365, 2852
MaeIII GTNAC 4 cut(s) 88, 392, 1526, 1651
MfeI CAATTG 2 cut(s) 248, 926
MflI RGATCY 3 cut(s) 1450, 1705, 1988
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 2 cut(s) 68, 2251
MmeI TCCRAC 5 cut(s) 610, 2429, 2705, 3069, 3292
Mph1103I ATGCAT 3 cut(s) 2494, 2629, 2926
MroNI GCCGGC 1 cut(s) 569
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 2395
MspI CCGG 7 cut(s) 19, 41, 570, 1157, 1313, 1412, 1748
MspR9I CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1749, 2811
MunI CAATTG 2 cut(s) 248, 926
Mva1269I GAATGC 2 cut(s) 556, 2764
MvaI CCWGG 3 cut(s) 387, 1719, 2811
MvnI CGCG 1 cut(s) 1724
MwoI GCNNNNNNNGC 7 cut(s) 322, 845, 1196, 1928, 2186, 2375, 2887
NaeI GCCGGC 1 cut(s) 571
NarI GGCGCC 1 cut(s) 325
NciI CCSGG 3 cut(s) 1158, 1412, 1749
NcoI CCATGG 1 cut(s) 765
NgoMIV GCCGGC 1 cut(s) 569
NlaIV GGNNCC 7 cut(s) 36, 326, 568, 641, 808, 1354, 2507
NmeAIII GCCGAG 3 cut(s) 301, 668, 1434
NmuCI GTSAC 3 cut(s) 88, 392, 1526
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 3 cut(s) 2494, 2629, 2926
NspI RCATGY 3 cut(s) 374, 1421, 2422
NspV TTCGAA 1 cut(s) 1487
PaqCI CACCTGC 1 cut(s) 2275
PceI AGGCCT 1 cut(s) 3323
PcsI WCGNNNNNNNCGW 1 cut(s) 2362
PctI GAATGC 2 cut(s) 556, 2764
PdiI GCCGGC 1 cut(s) 571
PflFI GACNNNGTC 1 cut(s) 2107
PleI GAGTC 2 cut(s) 67, 2250
PluTI GGCGCC 1 cut(s) 328
PmaCI CACGTG 1 cut(s) 2366
PmlI CACGTG 1 cut(s) 2366
PpsI GAGTC 2 cut(s) 67, 2250
Ppu21I YACGTR 1 cut(s) 2366
PshAI GACNNNNGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 2865
Psp1406I AACGTT 1 cut(s) 1363
Psp6I CCWGG 3 cut(s) 385, 1717, 2809
PspCI CACGTG 1 cut(s) 2366
PspEI GGTNACC 1 cut(s) 392
PspGI CCWGG 3 cut(s) 385, 1717, 2809
PspN4I GGNNCC 7 cut(s) 36, 326, 568, 641, 808, 1354, 2507
PsrI GAACNNNNNNTAC 4 cut(s) 476, 508, 2298, 2330
PstI CTGCAG 3 cut(s) 1153, 1741, 2128
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 1450, 1705, 1988
PsyI GACNNNGTC 1 cut(s) 2107
PvuII CAGCTG 1 cut(s) 2395
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 2865
SalI GTCGAC 1 cut(s) 448
SchI GAGTC 2 cut(s) 68, 2251
ScrFI CCNGG 6 cut(s) 387, 1158, 1412, 1719, 1749, 2811
SfaNI GCATC 9 cut(s) 853, 907, 1208, 1210, 2479, 2593, 2636, 2868, 3025
SfcI CTRYAG 3 cut(s) 1149, 1737, 2124
SfoI GGCGCC 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 1487
SinI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3305
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 910, 2283
SmoI CTYRAG 2 cut(s) 910, 2283
SpeI ACTAGT 1 cut(s) 2234
SseBI AGGCCT 1 cut(s) 3323
SspDI GGCGCC 1 cut(s) 324
SstI GAGCTC 1 cut(s) 2865
StuI AGGCCT 1 cut(s) 3323
StyD4I CCNGG 6 cut(s) 385, 1156, 1410, 1717, 1747, 2809
StyI CCWWGG 3 cut(s) 765, 1961, 3014
TaiI ACGT 3 cut(s) 1366, 2368, 2855
TaqI TCGA 7 cut(s) 96, 449, 998, 1487, 2968, 2983, 3148
TatI WGTACW 4 cut(s) 987, 1052, 1784, 2594
TauI GCSGC 2 cut(s) 763, 2884
TseFI GTSAC 3 cut(s) 88, 392, 1526
TseI GCWGC 8 cut(s) 262, 620, 845, 1190, 1739, 1742, 1876, 2588
Tsp45I GTSAC 3 cut(s) 88, 392, 1526
TspGWI ACGGA 2 cut(s) 1345, 3220
Tth111I GACNNNGTC 1 cut(s) 2107
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 9 cut(s) 34, 950, 1353, 2058, 2506, 2696, 2789, 2999, 3305
XapI RAATTY 4 cut(s) 381, 932, 1270, 2032
XbaI TCTAGA 1 cut(s) 2471
XceI RCATGY 3 cut(s) 374, 1421, 2422
XmaJI CCTAGG 1 cut(s) 3014
XmiI GTMKAC 3 cut(s) 449, 1237, 2842
Zsp2I ATGCAT 3 cut(s) 2494, 2629, 2926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.