AT1G33990

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013298
Physical Location & Seq
Forward (+)
12355557 .. 12358031
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33990.1

Sequence Viewer

Length: 1047 bp
ATGGGTAACAAGATAATCTCAATGATGAAGAAAGATAGCAAAGATGGAGGAGGAGGAGGATCTAAGAGCAAGAGAATGAATCGGTCTCAGAGGAAATTACTTGCTGATGAAGAGATGTTGCATCGTCGAGCTCTTTCCATGGCGATTCATCAAGCTCAGCTTTCTCAGAGATTTGATGGATCTATGTCTAGACGGGTCGGGTCTACTAGTACTAGGAAACGTACTCTCTCTGACCCGTTTTCTAATGGCAAGCAGGTACCTGATTTTTCAGAGAGCTTGATAGTGAAGAAATTTGTTCTGGTGCACGGTGAAGGATTTGGGGCATGGTGTTGGTACAAAATGGTTGCTTCATTGGAAGAGTCAGGACTGTCTCCTGTTACAGTTGACCTCACTGGGTGTGGATTTAATATGACTGACACAAATACTGTCTCTACCTTGGAGGAATATTCAAAACCGTTGATCGATCTCCTCGAAAATCTCCCCGAAGAAGAGAAGGTTATTCTAGTGGGTCACAGTACTGGAGGTGCTTCCATTTCATATGCGTTAGAGAGGTTTCCAGAGAAAATCTCTAAAGCTATATTCGTATGTGCAACAATGGTTTCTGATGGCCAAAGACCTTTTGATGTTTTCTCTGAAGAGCTTGGTTCCGCAGAGCGGTTCATGAAAGAGTCGCAGTTCTTGATTTATGGGAACGGTAAAGACAAGCCTCCTACGGGGTTCATGTTTGAGAAGCCACATATGAAAGGCTTGTATTTCAATCAATCACCCAACAAGGATATAGCATTGGCGATGATCTCAATGAGACCTGTACCACTCGGACCAATGATGGAGAAAGTATCTCTAACCGCAGAAAGATATGGGAAAGGGAGAAGATTCTACGTCCAGACGCTAGATGACCGTGCACTTTCACCAGATGTTCAAGAGAAGTTAGTGAGAGAGAACAGCCCTGAAGGAGTCTTCAAGATCAAAGGAAGTGATCATTGCCCTTTCTTCTCAAAGCCTCAATCTCTCCACAAAATCCTTCTGGAGATTGCACAGATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

348

Amino Acids

38.85

Weight (kDa)

8.97

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 98 - 335 6.6e-20 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 98 - 204 3.3e-13 alpha/beta hydrolase fold
Hydrolase_4 PF12146 98 - 205 1.7e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33990 AT4G09900
fragaria_vesca FvH4_5g32490
malus_domestica MD08G1216700.v1.1 MD15G1403900.v1.1
prunus_persica Prupe.1G550200_v2.0.a1
pyrus_communis pycom08g18760 pycom15g36120
rosa_chinensis RchiOBHm_Chr7g0229071
rosa_laevigata RLG00000001533
rosa_multiflora Rmu_sc0000271.1_g000022
rosa_roxburghii Rroxscaffold_3G00230770
rosa_rugosa Rorug07G0255400
rosa_samantha Rh7AG406500 Rh7BG385900 Rh7CG424300 Rh7DG400800
rosa_wichuraiana Rw7G033740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 256
AccBSI CCGCTC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 203
AciI CCGC 3 cut(s) 648, 655, 846
AclWI GGATC 2 cut(s) 67, 187
AcoI YGGCCR 1 cut(s) 607
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 2 cut(s) 654, 969
AdeI CACNNNGTG 1 cut(s) 396
AfaI GTAC 6 cut(s) 211, 223, 258, 335, 517, 810
AfiI CCNNNNNNNGG 2 cut(s) 654, 713
AgsI TTSAA 4 cut(s) 450, 757, 920, 961
AhlI ACTAGT 1 cut(s) 206
AluBI AGCT 6 cut(s) 131, 155, 160, 276, 575, 640
AluI AGCT 6 cut(s) 131, 155, 160, 276, 575, 640
Alw21I GWGCWC 3 cut(s) 133, 306, 904
Alw26I GTCTC 4 cut(s) 90, 375, 433, 796
Alw44I GTGCAC 2 cut(s) 302, 900
AlwI GGATC 2 cut(s) 67, 187
AoxI GGCC 1 cut(s) 607
ApaLI GTGCAC 2 cut(s) 302, 900
ApoI RAATTY 1 cut(s) 290
Asp718I GGTACC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 818
AsuHPI GGTGA 3 cut(s) 320, 756, 900
AvaII GGWCC 1 cut(s) 818
BaeGI GKGCMC 2 cut(s) 306, 904
BalI TGGCCA 1 cut(s) 609
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 949
Bbv12I GWGCWC 3 cut(s) 133, 306, 904
BccI CCATC 4 cut(s) 38, 170, 599, 820
BclI TGATCA 1 cut(s) 976
BcoDI GTCTC 4 cut(s) 90, 375, 433, 796
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 5 cut(s) 189, 207, 213, 503, 890
BfuAI ACCTGC 1 cut(s) 244
BlpI GCTNAGC 1 cut(s) 156
BmcAI AGTACT 2 cut(s) 211, 517
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 2 cut(s) 258, 646
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 949
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 2 cut(s) 540, 1046
Bpu1102I GCTNAGC 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 462
BsaI GGTCTC 2 cut(s) 90, 796
BsaJI CCNNGG 2 cut(s) 138, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 654, 713
Bse1I ACTGG 2 cut(s) 397, 523
Bse3DI GCAATG 1 cut(s) 979
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 138, 435
BseLI CCNNNNNNNGG 2 cut(s) 654, 713
BseMI GCAATG 1 cut(s) 979
BseMII CTCAG 3 cut(s) 101, 170, 179
BseNI ACTGG 2 cut(s) 397, 523
BseRI GAGGAG 4 cut(s) 63, 66, 69, 458
BseSI GKGCMC 2 cut(s) 306, 904
BshFI GGCC 1 cut(s) 609
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 3 cut(s) 133, 306, 904
BslI CCNNNNNNNGG 2 cut(s) 654, 713
BsmAI GTCTC 4 cut(s) 90, 375, 433, 796
BsnI GGCC 1 cut(s) 609
Bso31I GGTCTC 2 cut(s) 90, 796
Bsp1286I GDGCHC 3 cut(s) 133, 306, 904
Bsp143I GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 3 cut(s) 648, 655, 846
BspANI GGCC 1 cut(s) 609
BspCNI CTCAG 3 cut(s) 100, 169, 178
BspDI ATCGAT 1 cut(s) 462
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 2 cut(s) 258, 646
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 2 cut(s) 67, 187
BspQI GCTCTTC 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 2 cut(s) 90, 796
BsrBI CCGCTC 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 979
BsrI ACTGG 2 cut(s) 397, 523
BssECI CCNNGG 2 cut(s) 138, 435
BssMI GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
BssT1I CCWWGG 2 cut(s) 138, 435
Bst4CI ACNGT 8 cut(s) 308, 369, 382, 427, 456, 515, 695, 899
Bst6I CTCTTC 4 cut(s) 105, 351, 483, 630
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 4 cut(s) 63, 87, 156, 165
BstDSI CCRYGG 1 cut(s) 138
BstKTI GATC 7 cut(s) 62, 182, 462, 466, 795, 966, 979
BstMAI GTCTC 4 cut(s) 90, 375, 433, 796
BstMBI GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
BstSLI GKGCMC 2 cut(s) 306, 904
BstV2I GAAGAC 1 cut(s) 949
BstX2I RGATCY 2 cut(s) 59, 179
BstYI RGATCY 2 cut(s) 59, 179
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 1 cut(s) 609
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 138
BtgZI GCGATG 1 cut(s) 803
BtsIMutI CAGTG 1 cut(s) 390
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 251
CciI TCATGA 1 cut(s) 660
Cfr13I GGNCC 1 cut(s) 818
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 1 cut(s) 895
Csp6I GTAC 6 cut(s) 210, 222, 257, 334, 516, 809
CviAII CATG 4 cut(s) 139, 324, 661, 721
CviQI GTAC 6 cut(s) 210, 222, 257, 334, 516, 809
DdeI CTNAG 4 cut(s) 63, 87, 156, 165
DpnI GATC 7 cut(s) 61, 181, 461, 465, 794, 965, 978
DpnII GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
DraIII CACNNNGTG 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 607
Eam1104I CTCTTC 4 cut(s) 105, 351, 483, 630
EarI CTCTTC 4 cut(s) 105, 351, 483, 630
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 2 cut(s) 138, 435
Eco24I GRGCYC 1 cut(s) 133
Eco31I GGTCTC 2 cut(s) 90, 796
Eco47I GGWCC 1 cut(s) 818
Eco53kI GAGCTC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 654, 969
EcoICRI GAGCTC 1 cut(s) 131
EcoT14I CCWWGG 2 cut(s) 138, 435
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 138, 435
FaeI CATG 4 cut(s) 142, 327, 664, 724
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FatI CATG 4 cut(s) 138, 323, 660, 720
FauNDI CATATG 2 cut(s) 538, 738
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 133
FspBI CTAG 5 cut(s) 189, 207, 213, 503, 890
GsuI CTGGAG 2 cut(s) 540, 1046
HaeIII GGCC 1 cut(s) 609
HgaI GACGC 1 cut(s) 895
Hin1II CATG 4 cut(s) 142, 327, 664, 724
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 7 cut(s) 79, 145, 359, 668, 873, 954, 1039
HphI GGTGA 3 cut(s) 320, 756, 900
Hpy166II GTNNAC 4 cut(s) 204, 304, 385, 902
Hpy188I TCNGA 7 cut(s) 90, 168, 232, 271, 604, 634, 818
Hpy188III TCNNGA 9 cut(s) 189, 363, 557, 661, 679, 883, 920, 961, 1025
Hpy8I GTNNAC 4 cut(s) 204, 304, 385, 902
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 4 cut(s) 305, 487, 944, 1031
HpyCH4III ACNGT 8 cut(s) 308, 369, 382, 427, 456, 515, 695, 899
HpyCH4IV ACGT 2 cut(s) 220, 879
HpyCH4V TGCA 5 cut(s) 121, 304, 590, 902, 1034
HpyF3I CTNAG 4 cut(s) 63, 87, 156, 165
HpySE526I ACGT 2 cut(s) 220, 879
Hsp92II CATG 4 cut(s) 142, 327, 664, 724
KpnI GGTACC 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
LguI GCTCTTC 1 cut(s) 630
LweI GCATC 1 cut(s) 130
MaeI CTAG 5 cut(s) 189, 207, 213, 503, 890
MaeII ACGT 2 cut(s) 220, 879
MaeIII GTNAC 3 cut(s) 5, 376, 509
MalI GATC 7 cut(s) 61, 181, 461, 465, 794, 965, 978
MbiI CCGCTC 1 cut(s) 655
MboI GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
MflI RGATCY 2 cut(s) 59, 179
MhlI GDGCHC 3 cut(s) 133, 306, 904
MlsI TGGCCA 1 cut(s) 609
MluCI AATT 2 cut(s) 95, 290
MluNI TGGCCA 1 cut(s) 609
MlyI GAGTC 3 cut(s) 368, 677, 963
Mox20I TGGCCA 1 cut(s) 609
MscI TGGCCA 1 cut(s) 609
MseI TTAA 2 cut(s) 405, 1045
Msp20I TGGCCA 1 cut(s) 609
NcoI CCATGG 1 cut(s) 138
NdeI CATATG 2 cut(s) 538, 738
NdeII GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
NlaIII CATG 4 cut(s) 142, 327, 664, 724
NlaIV GGNNCC 2 cut(s) 258, 646
NmuCI GTSAC 1 cut(s) 509
PagI TCATGA 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 630
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PfeI GAWTC 4 cut(s) 79, 145, 873, 1039
PleI GAGTC 3 cut(s) 367, 676, 962
PpsI GAGTC 3 cut(s) 367, 676, 962
Psp124BI GAGCTC 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 258, 646
PspPI GGNCC 1 cut(s) 818
PsuI RGATCY 2 cut(s) 59, 179
RsaI GTAC 6 cut(s) 211, 223, 258, 335, 517, 810
RsaNI GTAC 6 cut(s) 210, 222, 257, 334, 516, 809
SacI GAGCTC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 405, 1045
Sau3AI GATC 7 cut(s) 59, 179, 459, 463, 792, 963, 976
Sau96I GGNCC 1 cut(s) 818
ScaI AGTACT 2 cut(s) 211, 517
SchI GAGTC 3 cut(s) 368, 677, 963
SduI GDGCHC 3 cut(s) 133, 306, 904
SfaNI GCATC 1 cut(s) 130
SinI GGWCC 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 2 cut(s) 95, 290
SsiI CCGC 3 cut(s) 648, 655, 846
SspI AATATT 1 cut(s) 446
SspMI CTAG 5 cut(s) 189, 207, 213, 503, 890
SstI GAGCTC 1 cut(s) 133
StyI CCWWGG 2 cut(s) 138, 435
TaaI ACNGT 8 cut(s) 308, 369, 382, 427, 456, 515, 695, 899
TaiI ACGT 2 cut(s) 223, 882
TaqI TCGA 3 cut(s) 127, 462, 471
TaqII GACCGA 1 cut(s) 72
TasI AATT 2 cut(s) 95, 290
TatI WGTACW 2 cut(s) 209, 515
TfiI GAWTC 4 cut(s) 79, 145, 873, 1039
Tru1I TTAA 2 cut(s) 405, 1045
Tru9I TTAA 2 cut(s) 405, 1045
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 1 cut(s) 509
Tsp45I GTSAC 1 cut(s) 509
TspRI CASTG 1 cut(s) 397
VneI GTGCAC 2 cut(s) 302, 900
VpaK11BI GGWCC 1 cut(s) 818
XapI RAATTY 1 cut(s) 290
XbaI TCTAGA 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 203
XspI CTAG 5 cut(s) 189, 207, 213, 503, 890
ZrmI AGTACT 2 cut(s) 211, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.