AT4G09900

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013298
Physical Location & Seq
Reverse (-)
6221628 .. 6224221
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G09900.1

Sequence Viewer

Length: 1050 bp
ATGGGTAACAGAGTAATCTGCATGAAGAAGAAAGATGTTGTTATCAGAAGTGGAGGAGATGGGTCCAGAAGCAAGAGAGTGAATCGTTCACAGAGGAAGTTGCTTGCTGATGAAGAGAATTTGCATCGAAGAGCTTTGTCTATGGCTATTCATCAAGCTCAAGTTTCTCAGAGATTTGATGGATCCATGTCTAGACGAATCGGGTCTACTAGCTCTAGAAGAGGAACACTCTCTGACTCATTTTCTAATAACAAGCAGGTTCCTGAGTTTTTGGAGAGCTTGAAGGTTAAGAAATTCGTTTTGGTGCATGGTGAAGGGTTTGGGGCATGGTGTTGGTACAAAACTATTGCGTCATTGGAGGAGTCTGGACTGTCTCCTGTTACGGTTGACCTCGCTGGATCTGGATTTAATATGACAGACGCAAATAGTGTTTCCACCTTAGAAGAATATTCAAAACCGTTGATCGAGCTCATTCAAAATCTTCCTGCTGAAGAAAAGGTGATTCTGGTAGGTCACAGTACTGGAGGGGCATGTGTTTCATATGCGTTAGAGCGGTTTCCAGAGAAAATCTCAAAAGCTATATTCATTTGTGCAACTATGGTTACTGATGGACAAAGACCCTTCGATGTCTTCGCTGATGAGCTTGGTTCTGCAGAACGGTTCATGAAAGAGTCACAGTTCTTGATCTATGGAAACGGCAAGGACAACCCCGCGACAGGGTTCATGTTCGAGAAACAACACATGAAAGGCTTGTACTTCAATCAGTCACCAAATAAGGACATTGCATTGTCAATGATCTCGATGCGGCCTGTACCATTAGGTCCCATGATGGAGAAGTTGTCACTTAGTGCAGAAAGATATGGGAAAGGTCGGAGATTCTATGTCCAGACGCTCGATGACTTGGCTCTTTCACCGGACGTTCAAGAGAAACTGGTTCGAGAGAATAGTCCCGAAGCAGTTTTTAAGATCAAAGGAAGTGATCATTGCCCATTCTTCTCTAAACCTCAGTCTCTTCACAAGATCCTTCTTGAAATAGCACAGATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

349

Amino Acids

38.93

Weight (kDa)

9.05

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 98 - 206 8.9e-10 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 99 - 336 1.4e-17 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 99 - 209 2.5e-13 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33990 AT4G09900
fragaria_vesca FvH4_5g32490
malus_domestica MD08G1216700.v1.1 MD15G1403900.v1.1
prunus_persica Prupe.1G550200_v2.0.a1
pyrus_communis pycom08g18760 pycom15g36120
rosa_chinensis RchiOBHm_Chr7g0229071
rosa_laevigata RLG00000001533
rosa_multiflora Rmu_sc0000271.1_g000022
rosa_roxburghii Rroxscaffold_3G00230770
rosa_rugosa Rorug07G0255400
rosa_samantha Rh7AG406500 Rh7BG385900 Rh7CG424300 Rh7DG400800
rosa_wichuraiana Rw7G033740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 553
AccI GTMKAC 1 cut(s) 206
AccII CGCG 1 cut(s) 713
AciI CCGC 3 cut(s) 553, 711, 805
AclWI GGATC 4 cut(s) 177, 190, 406, 1015
AcsI RAATTY 2 cut(s) 118, 293
AcuI CTGAAG 1 cut(s) 510
AdeI CACNNNGTG 1 cut(s) 848
AfaI GTAC 4 cut(s) 338, 520, 755, 813
AfiI CCNNNNNNNGG 2 cut(s) 716, 717
AgsI TTSAA 6 cut(s) 283, 453, 476, 760, 923, 1031
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 7 cut(s) 134, 158, 213, 279, 469, 578, 643
AluI AGCT 7 cut(s) 134, 158, 213, 279, 469, 578, 643
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 378, 1014
AlwI GGATC 4 cut(s) 177, 190, 406, 1015
AoxI GGCC 1 cut(s) 806
ApoI RAATTY 2 cut(s) 118, 293
AspS9I GGNCC 2 cut(s) 63, 821
AsuHPI GGTGA 4 cut(s) 323, 511, 759, 903
AvaII GGWCC 2 cut(s) 63, 821
BamHI GGATCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 471
BccI CCATC 4 cut(s) 53, 173, 602, 823
BceAI ACGGC 1 cut(s) 712
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 378, 1014
BfaI CTAG 3 cut(s) 192, 210, 216
BfmI CTRYAG 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 1 cut(s) 806
BlsI GCNGC 1 cut(s) 807
BmcAI AGTACT 1 cut(s) 520
Bme18I GGWCC 2 cut(s) 63, 821
BmgT120I GGNCC 2 cut(s) 63, 821
BmiI GGNNCC 4 cut(s) 64, 184, 261, 823
BmsI GCATC 2 cut(s) 133, 792
BpiI GAAGAC 1 cut(s) 622
BplI GAGNNNNNCTC 4 cut(s) 213, 245, 554, 586
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 913
BsaXI ACNNNNNCTCC 2 cut(s) 824, 854
Bsc4I CCNNNNNNNGG 2 cut(s) 716, 717
Bse1I ACTGG 2 cut(s) 526, 936
Bse3DI GCAATG 2 cut(s) 780, 982
BseLI CCNNNNNNNGG 2 cut(s) 716, 717
BseMI GCAATG 2 cut(s) 780, 982
BseMII CTCAG 3 cut(s) 182, 255, 1019
BseNI ACTGG 2 cut(s) 526, 936
BseRI GAGGAG 2 cut(s) 69, 374
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 1 cut(s) 808
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 1 cut(s) 914
BslFI GGGAC 2 cut(s) 807, 933
BslI CCNNNNNNNGG 2 cut(s) 716, 717
BsmAI GTCTC 2 cut(s) 378, 1014
BsmFI GGGAC 2 cut(s) 807, 933
BsnI GGCC 1 cut(s) 808
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
BspACI CCGC 3 cut(s) 553, 711, 805
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 3 cut(s) 181, 256, 1018
BspFNI CGCG 1 cut(s) 713
BspHI TCATGA 1 cut(s) 663
BspLI GGNNCC 4 cut(s) 64, 184, 261, 823
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 4 cut(s) 177, 190, 406, 1015
BspQI GCTCTTC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 553
BsrDI GCAATG 2 cut(s) 780, 982
BsrI ACTGG 2 cut(s) 526, 936
BssMI GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
Bst4CI ACNGT 6 cut(s) 372, 385, 459, 518, 660, 678
Bst6I CTCTTC 4 cut(s) 108, 124, 214, 1017
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 5 cut(s) 168, 264, 439, 845, 1005
BstFNI CGCG 1 cut(s) 713
BstKTI GATC 8 cut(s) 185, 401, 465, 687, 798, 969, 982, 1023
BstMAI GTCTC 2 cut(s) 378, 1014
BstMBI GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
BstNSI RCATGY 1 cut(s) 534
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 713
BstV2I GAAGAC 1 cut(s) 622
BstX2I RGATCY 3 cut(s) 182, 398, 1020
BstYI RGATCY 3 cut(s) 182, 398, 1020
BsuRI GGCC 1 cut(s) 808
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 1 cut(s) 105
CciI TCATGA 1 cut(s) 663
Cfr13I GGNCC 2 cut(s) 63, 821
CseI GACGC 3 cut(s) 339, 428, 898
Csp6I GTAC 4 cut(s) 337, 519, 754, 812
CviAII CATG 9 cut(s) 22, 187, 308, 327, 531, 664, 724, 742, 826
CviQI GTAC 4 cut(s) 337, 519, 754, 812
DdeI CTNAG 5 cut(s) 168, 264, 439, 845, 1005
DpnI GATC 8 cut(s) 184, 400, 464, 686, 797, 968, 981, 1022
DpnII GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
DraIII CACNNNGTG 1 cut(s) 848
Eam1104I CTCTTC 4 cut(s) 108, 124, 214, 1017
EarI CTCTTC 4 cut(s) 108, 124, 214, 1017
Ecl136II GAGCTC 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 63, 821
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 821
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 9 cut(s) 25, 190, 311, 330, 534, 667, 727, 745, 829
FaqI GGGAC 2 cut(s) 807, 933
FatI CATG 9 cut(s) 21, 186, 307, 326, 530, 663, 723, 741, 825
FauI CCCGC 1 cut(s) 718
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 1 cut(s) 806
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 806
FspBI CTAG 3 cut(s) 192, 210, 216
GluI GCNGC 1 cut(s) 806
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 1 cut(s) 808
HapII CCGG 1 cut(s) 914
HgaI GACGC 3 cut(s) 339, 428, 898
Hin1II CATG 9 cut(s) 25, 190, 311, 330, 534, 667, 727, 745, 829
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 8 cut(s) 82, 198, 236, 362, 502, 671, 876, 1042
HpaII CCGG 1 cut(s) 914
HphI GGTGA 4 cut(s) 323, 511, 759, 903
Hpy166II GTNNAC 3 cut(s) 89, 207, 388
Hpy188I TCNGA 4 cut(s) 47, 171, 235, 873
Hpy8I GTNNAC 3 cut(s) 89, 207, 388
HpyAV CCTTC 4 cut(s) 277, 308, 631, 1034
HpyCH4III ACNGT 6 cut(s) 372, 385, 459, 518, 660, 678
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 7 cut(s) 21, 124, 307, 593, 653, 785, 851
HpyF3I CTNAG 5 cut(s) 168, 264, 439, 845, 1005
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 9 cut(s) 25, 190, 311, 330, 534, 667, 727, 745, 829
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
LguI GCTCTTC 1 cut(s) 124
LweI GCATC 2 cut(s) 133, 792
MaeI CTAG 3 cut(s) 192, 210, 216
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 7 cut(s) 5, 379, 512, 601, 672, 765, 840
MalI GATC 8 cut(s) 184, 400, 464, 686, 797, 968, 981, 1022
MbiI CCGCTC 1 cut(s) 553
MboI GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
MflI RGATCY 3 cut(s) 182, 398, 1020
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 2 cut(s) 118, 293
MlyI GAGTC 3 cut(s) 230, 371, 680
MmeI TCCRAC 1 cut(s) 851
MnlI CCTC 7 cut(s) 47, 87, 215, 352, 401, 518, 1014
MseI TTAA 4 cut(s) 288, 408, 963, 1048
MspI CCGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 713
NdeI CATATG 1 cut(s) 541
NdeII GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
NlaIII CATG 9 cut(s) 25, 190, 311, 330, 534, 667, 727, 745, 829
NlaIV GGNNCC 4 cut(s) 64, 184, 261, 823
NmuCI GTSAC 4 cut(s) 512, 672, 765, 840
NspI RCATGY 1 cut(s) 534
PagI TCATGA 1 cut(s) 663
PciSI GCTCTTC 1 cut(s) 124
PfeI GAWTC 5 cut(s) 82, 198, 502, 876, 1042
PkrI GCNGC 1 cut(s) 807
PleI GAGTC 3 cut(s) 230, 370, 679
PpsI GAGTC 3 cut(s) 230, 370, 679
PpuMI RGGWCCY 1 cut(s) 821
Psp124BI GAGCTC 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 821
PspN4I GGNNCC 4 cut(s) 64, 184, 261, 823
PspPI GGNCC 2 cut(s) 63, 821
PspPPI RGGWCCY 1 cut(s) 821
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 3 cut(s) 182, 398, 1020
RsaI GTAC 4 cut(s) 338, 520, 755, 813
RsaNI GTAC 4 cut(s) 337, 519, 754, 812
SacI GAGCTC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 124
SaqAI TTAA 4 cut(s) 288, 408, 963, 1048
SatI GCNGC 1 cut(s) 806
Sau3AI GATC 8 cut(s) 182, 398, 462, 684, 795, 966, 979, 1020
Sau96I GGNCC 2 cut(s) 63, 821
ScaI AGTACT 1 cut(s) 520
SchI GAGTC 3 cut(s) 230, 371, 680
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 133, 792
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 2 cut(s) 63, 821
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 2 cut(s) 118, 293
SsiI CCGC 3 cut(s) 553, 711, 805
SspI AATATT 1 cut(s) 449
SspMI CTAG 3 cut(s) 192, 210, 216
SstI GAGCTC 1 cut(s) 471
TaaI ACNGT 6 cut(s) 372, 385, 459, 518, 660, 678
TaiI ACGT 1 cut(s) 921
TaqI TCGA 7 cut(s) 127, 465, 624, 729, 800, 894, 937
TasI AATT 2 cut(s) 118, 293
TatI WGTACW 2 cut(s) 518, 753
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 5 cut(s) 82, 198, 502, 876, 1042
Tru1I TTAA 4 cut(s) 288, 408, 963, 1048
Tru9I TTAA 4 cut(s) 288, 408, 963, 1048
TseFI GTSAC 4 cut(s) 512, 672, 765, 840
Tsp45I GTSAC 4 cut(s) 512, 672, 765, 840
TspDTI ATGAA 9 cut(s) 38, 126, 140, 528, 574, 652, 680, 712, 758
VpaK11BI GGWCC 2 cut(s) 63, 821
XapI RAATTY 2 cut(s) 118, 293
XbaI TCTAGA 2 cut(s) 191, 215
XceI RCATGY 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 3 cut(s) 192, 210, 216
ZrmI AGTACT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.