MD08G1216700.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013298
Physical Location & Seq
Reverse (-)
27945018 .. 27949358
4341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1216700.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGGTAATCGATTTATTTGCATGTCAAAGAAGGACACCAAAGAAGTAGGATCGAGAAGCAAGAGAATGAATCGCTCGCAGAGGAAACTGGTCGCGGAGGAAGACTTGCTGCACAGGCAAGCTCTGTCTATGGTTCTTCAACAACATCAATCGTCGCAGAGGTTCGACGGATCCATGTCCAGGAGGATTGGGGGGTCCACGAGCTCCAGGAGACTCGCCTCTGATCCATTGACTAATGGGAAGCAGGGACCTGAATTTTTGGAGAAGCTTACGTCAAAAAAGTTCATTCTTGTACATGGAGGAGGATTTGGGGCATGGTGTTGGTACAAAACAATTGCTCTACTAGAGGAAGCGGGATTGCTTGCAATCACTTTTGATCTCAAGGGTTCTGGTATTGATCTCACAGATACGAACTCTGTAGCTACGCTGGAAGAGTATTCGAAGCCTTTGATTGACTATCTACAAAATCTTCCAGAGGATGAAAAGGTCATCTTGGTTGGTCACAGTAGTGGGGGTGCCTGCGTATCTTATGCACTGGAGCACTTTTCACAGAAAATTTCAAAAGCAGTTTTCATCTGTGCTACTATGGTGTCTGATGGCCAGAGACCCTTTGATGTGTTTGCTGAAGAGCTTGGTTCTGCAGAACAATTTATGCAAGGATCAAAGTTTTTGATTCATGGGAATGGGAACGAAAAGCCTCCTACAGGATTCATGTTTGAGAAAGAGCAGATGAAAGGGTTATACTTCAACCAATCTAATACGAAGGATGTTGCTCTGGCCATGGTTTCCATGAGACCTATCCCACTAGGTCCGATCATGGAAAAACTGTCATTGACGCCTGAAAATTATGGAACTAGCCGGCGGTTCTTCATTCAAACACTAGACGATCGGGCACTTTCACCAGATGTCCAAGAAAAGCTAGTAAGGGAAAACCCACCGGAGGGAGTGTACAAGATAAAAGGGAGCGACCACTGCCCGTTCTTCTCAAAGCCACAATCATTGCACAAAATGCTGGTGGAAATTGCTCAAATTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

346

Amino Acids

38.51

Weight (kDa)

8.46

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 94 - 202 2.6e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 95 - 221 7.1e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 96 - 332 1.1e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33990 AT4G09900
fragaria_vesca FvH4_5g32490
malus_domestica MD08G1216700.v1.1 MD15G1403900.v1.1
prunus_persica Prupe.1G550200_v2.0.a1
pyrus_communis pycom08g18760 pycom15g36120
rosa_chinensis RchiOBHm_Chr7g0229071
rosa_laevigata RLG00000001533
rosa_multiflora Rmu_sc0000271.1_g000022
rosa_roxburghii Rroxscaffold_3G00230770
rosa_rugosa Rorug07G0255400
rosa_samantha Rh7AG406500 Rh7BG385900 Rh7CG424300 Rh7DG400800
rosa_wichuraiana Rw7G033740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 515
AccII CGCG 1 cut(s) 95
AciI CCGC 3 cut(s) 95, 353, 862
AclWI GGATC 5 cut(s) 58, 165, 178, 218, 667
AcoI YGGCCR 2 cut(s) 598, 777
AcsI RAATTY 3 cut(s) 254, 555, 1029
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 836
AfaI GTAC 3 cut(s) 294, 326, 950
AfiI CCNNNNNNNGG 4 cut(s) 180, 704, 940, 941
AgsI TTSAA 4 cut(s) 140, 561, 748, 875
AjnI CCWGG 2 cut(s) 179, 206
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 6 cut(s) 122, 204, 268, 422, 631, 919
AluI AGCT 6 cut(s) 122, 204, 268, 422, 631, 919
Alw21I GWGCWC 2 cut(s) 206, 543
Alw26I GTCTC 3 cut(s) 205, 598, 787
AlwI GGATC 5 cut(s) 58, 165, 178, 218, 667
AoxI GGCC 2 cut(s) 598, 777
ApeKI GCWGC 1 cut(s) 109
ApoI RAATTY 3 cut(s) 254, 555, 1029
AspS9I GGNCC 3 cut(s) 195, 248, 809
AsuHPI GGTGA 1 cut(s) 891
AsuII TTCGAA 1 cut(s) 440
AvaII GGWCC 3 cut(s) 195, 248, 809
BaeGI GKGCMC 1 cut(s) 895
BalI TGGCCA 2 cut(s) 600, 779
BamHI GGATCC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 206
BauI CACGAG 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 206, 543
BbvI GCAGC 1 cut(s) 96
BccI CCATC 1 cut(s) 590
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 181, 208
BcoDI GTCTC 3 cut(s) 205, 598, 787
BfaI CTAG 5 cut(s) 344, 806, 855, 881, 920
BfmI CTRYAG 3 cut(s) 417, 639, 702
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
Bme1390I CCNGG 2 cut(s) 181, 208
Bme18I GGWCC 3 cut(s) 195, 248, 809
BmgT120I GGNCC 3 cut(s) 195, 248, 809
BmiI GGNNCC 4 cut(s) 172, 196, 249, 517
BmrFI CCNGG 2 cut(s) 181, 208
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 190, 557
Bpu14I TTCGAA 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 365
Bsa29I ATCGAT 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 836
BsaI GGTCTC 2 cut(s) 598, 787
BsaJI CCNNGG 1 cut(s) 780
BsaWI WCCGGW 1 cut(s) 937
BsaXI ACNNNNNCTCC 2 cut(s) 932, 962
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 704, 940, 941
Bse118I RCCGGY 1 cut(s) 858
Bse1I ACTGG 2 cut(s) 93, 540
Bse3DI GCAATG 1 cut(s) 998
BseBI CCWGG 2 cut(s) 181, 208
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 2 cut(s) 484, 772
BseLI CCNNNNNNNGG 4 cut(s) 180, 704, 940, 941
BseMI GCAATG 1 cut(s) 998
BseNI ACTGG 2 cut(s) 93, 540
BseRI GAGGAG 1 cut(s) 315
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 95
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 2 cut(s) 600, 779
BshNI GGYRCC 1 cut(s) 515
BshVI ATCGAT 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 206, 543
BsiSI CCGG 2 cut(s) 859, 938
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 4 cut(s) 180, 704, 940, 941
BsmAI GTCTC 3 cut(s) 205, 598, 787
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 2 cut(s) 600, 779
Bso31I GGTCTC 2 cut(s) 598, 787
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 206, 543, 895
Bsp1407I TGTACA 2 cut(s) 292, 948
Bsp143I GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
Bsp19I CCATGG 1 cut(s) 780
BspACI CCGC 3 cut(s) 95, 353, 862
BspANI GGCC 2 cut(s) 600, 779
BspDI ATCGAT 1 cut(s) 10
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 4 cut(s) 172, 196, 249, 517
BspMAI CTGCAG 1 cut(s) 643
BspPI GGATC 5 cut(s) 58, 165, 178, 218, 667
BspQI GCTCTTC 1 cut(s) 621
BspT104I TTCGAA 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 2 cut(s) 598, 787
BsrDI GCAATG 1 cut(s) 998
BsrFI RCCGGY 1 cut(s) 858
BsrGI TGTACA 2 cut(s) 292, 948
BsrI ACTGG 2 cut(s) 93, 540
BssAI RCCGGY 1 cut(s) 858
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
BssNI GRCGYC 1 cut(s) 836
BssSI CACGAG 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 780
Bst2BI CACGAG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 181, 208
Bst4CI ACNGT 2 cut(s) 506, 828
Bst6I CTCTTC 2 cut(s) 426, 621
BstACI GRCGYC 1 cut(s) 836
BstAPI GCANNNNNTGC 1 cut(s) 1009
BstAUI TGTACA 2 cut(s) 292, 948
BstBI TTCGAA 1 cut(s) 440
BstC8I GCNNGC 5 cut(s) 77, 120, 363, 520, 860
BstDSI CCRYGG 1 cut(s) 780
BstENI CCTNNNNNAGG 1 cut(s) 702
BstF5I GGATG 2 cut(s) 484, 772
BstFNI CGCG 1 cut(s) 95
BstKTI GATC 8 cut(s) 53, 173, 226, 379, 400, 662, 816, 889
BstMAI GTCTC 3 cut(s) 205, 598, 787
BstMBI GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 3 cut(s) 115, 972, 1009
BstNI CCWGG 2 cut(s) 181, 208
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 179, 206
BstSFI CTRYAG 3 cut(s) 417, 639, 702
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 2 cut(s) 600, 779
BsuTUI ATCGAT 1 cut(s) 10
BtgI CCRYGG 1 cut(s) 780
BtsCI GGATG 2 cut(s) 484, 772
BtsI GCAGTG 1 cut(s) 970
BtsIMutI CAGTG 2 cut(s) 533, 970
Cac8I GCNNGC 5 cut(s) 77, 120, 363, 520, 860
Cfr10I RCCGGY 1 cut(s) 858
Cfr13I GGNCC 3 cut(s) 195, 248, 809
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 1 cut(s) 844
Csp6I GTAC 3 cut(s) 293, 325, 949
CspCI CAANNNNNGTGG 2 cut(s) 981, 1016
CviAII CATG 9 cut(s) 22, 175, 296, 315, 677, 712, 781, 790, 817
CviQI GTAC 3 cut(s) 293, 325, 949
DpnI GATC 8 cut(s) 52, 172, 225, 378, 399, 661, 815, 888
DpnII GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
EaeI YGGCCR 2 cut(s) 598, 777
Eam1104I CTCTTC 2 cut(s) 426, 621
EarI CTCTTC 2 cut(s) 426, 621
Ecl136II GAGCTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 2 cut(s) 598, 787
Eco47I GGWCC 3 cut(s) 195, 248, 809
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 204
EcoNI CCTNNNNNAGG 1 cut(s) 702
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 179, 206
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 780
FaeI CATG 9 cut(s) 25, 178, 299, 318, 680, 715, 784, 793, 820
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FaqI GGGAC 1 cut(s) 261
FatI CATG 9 cut(s) 21, 174, 295, 314, 676, 711, 780, 789, 816
FauI CCCGC 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 2 cut(s) 491, 779
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 5 cut(s) 344, 806, 855, 881, 920
GluI GCNGC 1 cut(s) 110
GsuI CTGGAG 2 cut(s) 190, 557
HaeIII GGCC 2 cut(s) 600, 779
HapII CCGG 2 cut(s) 859, 938
HgaI GACGC 1 cut(s) 844
Hin1I GRCGYC 1 cut(s) 836
Hin1II CATG 9 cut(s) 25, 178, 299, 318, 680, 715, 784, 793, 820
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 4 cut(s) 70, 213, 673, 708
HpaII CCGG 2 cut(s) 859, 938
HphI GGTGA 1 cut(s) 891
Hpy166II GTNNAC 2 cut(s) 198, 949
Hpy188I TCNGA 3 cut(s) 223, 595, 813
Hpy188III TCNNGA 2 cut(s) 54, 473
Hpy8I GTNNAC 2 cut(s) 198, 949
Hpy99I CGWCG 2 cut(s) 157, 170
HpyAV CCTTC 2 cut(s) 25, 757
HpyCH4III ACNGT 2 cut(s) 506, 828
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 7 cut(s) 21, 112, 365, 533, 641, 655, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 972, 1009
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 1 cut(s) 836
Hsp92II CATG 9 cut(s) 25, 178, 299, 318, 680, 715, 784, 793, 820
KroI GCCGGC 1 cut(s) 858
KroNI GCCGGC 1 cut(s) 860
Kzo9I GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 3 cut(s) 209, 538, 963
Lsp1109I GCAGC 1 cut(s) 96
MaeI CTAG 5 cut(s) 344, 806, 855, 881, 920
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 1 cut(s) 500
MalI GATC 8 cut(s) 52, 172, 225, 378, 399, 661, 815, 888
MboI GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
MboII GAAGA 7 cut(s) 113, 128, 443, 461, 638, 859, 973
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 3 cut(s) 206, 543, 895
MlsI TGGCCA 2 cut(s) 600, 779
MluCI AATT 7 cut(s) 254, 333, 555, 647, 844, 1020, 1029
MluNI TGGCCA 2 cut(s) 600, 779
MlyI GAGTC 1 cut(s) 207
Mox20I TGGCCA 2 cut(s) 600, 779
MroNI GCCGGC 1 cut(s) 858
MscI TGGCCA 2 cut(s) 600, 779
MslI CAYNNNNRTG 2 cut(s) 507, 681
Msp20I TGGCCA 2 cut(s) 600, 779
MspI CCGG 2 cut(s) 859, 938
MspR9I CCNGG 2 cut(s) 181, 208
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 2 cut(s) 181, 208
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 3 cut(s) 115, 972, 1009
NaeI GCCGGC 1 cut(s) 860
NcoI CCATGG 1 cut(s) 780
NdeII GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
NgoMIV GCCGGC 1 cut(s) 858
NlaIII CATG 9 cut(s) 25, 178, 299, 318, 680, 715, 784, 793, 820
NlaIV GGNNCC 4 cut(s) 172, 196, 249, 517
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 25
NspV TTCGAA 1 cut(s) 440
OliI CACNNNNGTG 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 621
PdiI GCCGGC 1 cut(s) 860
PfeI GAWTC 3 cut(s) 70, 673, 708
PfoI TCCNGGA 2 cut(s) 179, 206
PkrI GCNGC 1 cut(s) 111
Ple19I CGATCG 1 cut(s) 889
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 248
Psp124BI GAGCTC 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 179, 206
PspGI CCWGG 2 cut(s) 179, 206
PspN4I GGNNCC 4 cut(s) 172, 196, 249, 517
PspPI GGNCC 3 cut(s) 195, 248, 809
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 643
PsuI RGATCY 1 cut(s) 170
PvuI CGATCG 1 cut(s) 889
RsaI GTAC 3 cut(s) 294, 326, 950
RsaNI GTAC 3 cut(s) 293, 325, 949
RseI CAYNNNNRTG 2 cut(s) 507, 681
SacI GAGCTC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 621
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 50, 170, 223, 376, 397, 659, 813, 886
Sau96I GGNCC 3 cut(s) 195, 248, 809
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 181, 208
SduI GDGCHC 3 cut(s) 206, 543, 895
SfcI CTRYAG 3 cut(s) 417, 639, 702
SfuI TTCGAA 1 cut(s) 440
SinI GGWCC 3 cut(s) 195, 248, 809
SmiMI CAYNNNNRTG 2 cut(s) 507, 681
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 7 cut(s) 254, 333, 555, 647, 844, 1020, 1029
SsiI CCGC 3 cut(s) 95, 353, 862
SspMI CTAG 5 cut(s) 344, 806, 855, 881, 920
SstI GAGCTC 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 179, 206
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 2 cut(s) 506, 828
TaiI ACGT 1 cut(s) 275
TaqI TCGA 4 cut(s) 10, 53, 165, 440
TasI AATT 7 cut(s) 254, 333, 555, 647, 844, 1020, 1029
TatI WGTACW 2 cut(s) 292, 948
TfiI GAWTC 3 cut(s) 70, 673, 708
TscAI CASTG 2 cut(s) 540, 977
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 8 cut(s) 83, 274, 495, 562, 665, 700, 746, 859
TspGWI ACGGA 2 cut(s) 183, 1023
TspRI CASTG 2 cut(s) 540, 977
VpaK11BI GGWCC 3 cut(s) 195, 248, 809
XagI CCTNNNNNAGG 1 cut(s) 702
XapI RAATTY 3 cut(s) 254, 555, 1029
XceI RCATGY 1 cut(s) 25
XspI CTAG 5 cut(s) 344, 806, 855, 881, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.