FvH4_5g32490

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013298
Physical Location & Seq
Forward (+)
23444928 .. 23448963
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32490.t1

Sequence Viewer

Length: 1035 bp
ATGGGTAACCGATTTATTTGCATGTCAAAGAAGGAAACCAAAGAAAATGGATCCAGAAGTAAGAGGGTGAATCGTTCACAGAGGAAGCTGCTTGCAGAAGATGAGTTTTTGCACAGGCAAGCTCTGTCTATGGCTATTCATCAGCACCAATTGTCCCAGAGGTTTGATGGATCCATGTCTAGGAGAATTAATGGCTCCACCAGCTCTAGGAGAACCACTCTTCCCGAATCAGTAGCAAAGCAGGTACCTGAAAACTTGGACAACATAACAACAAAAAAGTTTATTTTAGTACATGGAGAAGGCTTTGGAGCATGGTGTTGGTACAAAACAATTGCTTTGCTAGAGGAAGTAGGATTGCTTCCCACCACCTTTGATCTCAAGGGTTCTGGTATTGATCTAACAGATTCTAACTCTGTTACTACTTTAGCAGAGTATTCAAAACCGTTGATTGACTATCTACAAAACCTTCCAGAGGATGAAAAGGTTATTTTGGTTGGTCACAGTACTGGTGGTGCCTGTGTTTCTTATGCTCTGGAGCACTTTCCGCAAAAAATTTCTAAAGCAATATTCATCTGTGCCACTATGGTGTCTAATGATCAGAGACCGTTTGATGTATTTGCTGAAGAGCTTGGTTCAGCGGAAGATTTTATGCAACAATCAAAGTTCATGATTCATGGAAATGGTAAAGACCAGCCTCCTACAGGATTTATGTTTGAGAAAGAACAGATGAAAGGGTTATATTTCAATCAATCTCCTGCAAAGGATGTTGCTCTGGCCATGGTGTCCATGAGACCAATCCCACTGGGTCCAATCATGGAGAAACTGTCTCTGTCGCCTGAGAAGTATGGAACTGGCCGGCGCTTCTTCATTCAAACATTAGATGATCGAGCACTTTCACCAGATGTCCAAGAAAAGCTGGTGAGGGAAAATCCACCTGAGGGAGTTTTCAAGATAAAAAGCAGCGACCACTGCCCATTCTTCTCAAAGCCAACGTCACTGCACAAAATTTTGGTTGAAATTGCTCAAATCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

345

Amino Acids

38.68

Weight (kDa)

8.18

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 93 - 201 2e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 94 - 206 2.3e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 95 - 330 5.7e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33990 AT4G09900
fragaria_vesca FvH4_5g32490
malus_domestica MD08G1216700.v1.1 MD15G1403900.v1.1
prunus_persica Prupe.1G550200_v2.0.a1
pyrus_communis pycom08g18760 pycom15g36120
rosa_chinensis RchiOBHm_Chr7g0229071
rosa_laevigata RLG00000001533
rosa_multiflora Rmu_sc0000271.1_g000022
rosa_roxburghii Rroxscaffold_3G00230770
rosa_rugosa Rorug07G0255400
rosa_samantha Rh7AG406500 Rh7BG385900 Rh7CG424300 Rh7DG400800
rosa_wichuraiana Rw7G033740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 2 cut(s) 244, 512
AciI CCGC 2 cut(s) 545, 638
AclWI GGATC 4 cut(s) 45, 58, 165, 178
AcoI YGGCCR 2 cut(s) 774, 853
AcsI RAATTY 2 cut(s) 552, 1005
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 4 cut(s) 246, 291, 323, 505
AfiI CCNNNNNNNGG 5 cut(s) 180, 207, 472, 701, 938
AgsI TTSAA 5 cut(s) 438, 745, 872, 949, 1016
AleI CACNNNNGTG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 205, 237
AluBI AGCT 5 cut(s) 88, 122, 204, 628, 916
AluI AGCT 5 cut(s) 88, 122, 204, 628, 916
Alw21I GWGCWC 2 cut(s) 540, 892
Alw26I GTCTC 3 cut(s) 595, 784, 831
AlwI GGATC 4 cut(s) 45, 58, 165, 178
AoxI GGCC 2 cut(s) 774, 853
ApeKI GCWGC 2 cut(s) 88, 960
ApoI RAATTY 2 cut(s) 552, 1005
ArsI GACNNNNNNTTYG 2 cut(s) 977, 1009
AseI ATTAAT 1 cut(s) 189
Asp718I GGTACC 1 cut(s) 244
AspLEI GCGC 1 cut(s) 861
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 3 cut(s) 79, 888, 931
AvaII GGWCC 1 cut(s) 806
AxyI CCTNAGG 1 cut(s) 936
BalI TGGCCA 1 cut(s) 776
BamHI GGATCC 2 cut(s) 50, 170
BanI GGYRCC 2 cut(s) 244, 512
BarI GAAGNNNNNNTAC 2 cut(s) 342, 374
Bbv12I GWGCWC 2 cut(s) 540, 892
BbvI GCAGC 2 cut(s) 75, 972
BccI CCATC 1 cut(s) 161
BcgI CGANNNNNNTGC 1 cut(s) 34
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 3 cut(s) 595, 784, 831
BfaI CTAG 3 cut(s) 180, 207, 341
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 862
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 2 cut(s) 89, 961
BlsI GCNGC 2 cut(s) 90, 962
BmcAI AGTACT 1 cut(s) 505
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 6 cut(s) 52, 172, 196, 246, 514, 807
BmrI ACTGGG 1 cut(s) 812
BmuI ACTGGG 1 cut(s) 812
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpmI CTGGAG 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 362
BsaI GGTCTC 2 cut(s) 595, 784
BsaJI CCNNGG 1 cut(s) 777
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 207, 472, 701, 938
Bse118I RCCGGY 1 cut(s) 855
Bse1I ACTGG 3 cut(s) 511, 807, 856
Bse21I CCTNAGG 1 cut(s) 936
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 2 cut(s) 481, 769
BseLI CCNNNNNNNGG 5 cut(s) 180, 207, 472, 701, 938
BseMII CTCAG 2 cut(s) 828, 927
BseNI ACTGG 3 cut(s) 511, 807, 856
BseXI GCAGC 2 cut(s) 75, 972
BsgI GTGCAG 1 cut(s) 983
BshFI GGCC 2 cut(s) 776, 855
BshNI GGYRCC 2 cut(s) 244, 512
BsiHKAI GWGCWC 2 cut(s) 540, 892
BsiSI CCGG 1 cut(s) 856
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 5 cut(s) 180, 207, 472, 701, 938
BsmAI GTCTC 3 cut(s) 595, 784, 831
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 2 cut(s) 776, 855
Bso31I GGTCTC 2 cut(s) 595, 784
Bsp1286I GDGCHC 2 cut(s) 540, 892
Bsp143I GATC 6 cut(s) 50, 170, 373, 394, 595, 883
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 2 cut(s) 545, 638
BspANI GGCC 2 cut(s) 776, 855
BspCNI CTCAG 2 cut(s) 829, 928
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 6 cut(s) 52, 172, 196, 246, 514, 807
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 4 cut(s) 45, 58, 165, 178
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 2 cut(s) 244, 512
BspTNI GGTCTC 2 cut(s) 595, 784
BsrFI RCCGGY 1 cut(s) 855
BsrI ACTGG 3 cut(s) 511, 807, 856
BssAI RCCGGY 1 cut(s) 855
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 6 cut(s) 50, 170, 373, 394, 595, 883
BssT1I CCWWGG 1 cut(s) 777
Bst4CI ACNGT 4 cut(s) 444, 503, 606, 825
Bst6I CTCTTC 2 cut(s) 225, 618
BstC8I GCNNGC 3 cut(s) 93, 120, 857
BstDEI CTNAG 2 cut(s) 837, 936
BstDSI CCRYGG 1 cut(s) 777
BstEII GGTNACC 1 cut(s) 5
BstENI CCTNNNNNAGG 2 cut(s) 470, 699
BstF5I GGATG 2 cut(s) 481, 769
BstH2I RGCGCY 1 cut(s) 862
BstHHI GCGC 1 cut(s) 861
BstKTI GATC 6 cut(s) 53, 173, 376, 397, 598, 886
BstMAI GTCTC 3 cut(s) 595, 784, 831
BstMBI GATC 6 cut(s) 50, 170, 373, 394, 595, 883
BstMWI GCNNNNNNNGC 3 cut(s) 201, 544, 969
BstNSI RCATGY 1 cut(s) 25
BstPI GGTNACC 1 cut(s) 5
BstSFI CTRYAG 1 cut(s) 699
BstV1I GCAGC 2 cut(s) 75, 972
BstX2I RGATCY 2 cut(s) 50, 170
BstYI RGATCY 2 cut(s) 50, 170
Bsu36I CCTNAGG 1 cut(s) 936
BsuRI GGCC 2 cut(s) 776, 855
BtgI CCRYGG 1 cut(s) 777
BtsCI GGATG 2 cut(s) 481, 769
BtsI GCAGTG 2 cut(s) 967, 995
BtsIMutI CAGTG 3 cut(s) 800, 967, 995
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 3 cut(s) 93, 120, 857
CciI TCATGA 1 cut(s) 666
CfoI GCGC 1 cut(s) 861
Cfr10I RCCGGY 1 cut(s) 855
Cfr13I GGNCC 1 cut(s) 806
Csp6I GTAC 4 cut(s) 245, 290, 322, 504
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 9 cut(s) 22, 175, 293, 312, 667, 674, 778, 787, 814
CviQI GTAC 4 cut(s) 245, 290, 322, 504
DdeI CTNAG 2 cut(s) 837, 936
DpnI GATC 6 cut(s) 52, 172, 375, 396, 597, 885
DpnII GATC 6 cut(s) 50, 170, 373, 394, 595, 883
EaeI YGGCCR 2 cut(s) 774, 853
Eam1104I CTCTTC 2 cut(s) 225, 618
EarI CTCTTC 2 cut(s) 225, 618
Eco130I CCWWGG 1 cut(s) 777
Eco31I GGTCTC 2 cut(s) 595, 784
Eco47I GGWCC 1 cut(s) 806
Eco57I CTGAAG 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 936
Eco91I GGTNACC 1 cut(s) 5
EcoNI CCTNNNNNAGG 2 cut(s) 470, 699
EcoO65I GGTNACC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 9 cut(s) 25, 178, 296, 315, 670, 677, 781, 790, 817
FaqI GGGAC 1 cut(s) 139
FatI CATG 9 cut(s) 21, 174, 292, 311, 666, 673, 777, 786, 813
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 2 cut(s) 89, 961
FokI GGATG 2 cut(s) 488, 776
Fsp4HI GCNGC 2 cut(s) 89, 961
FspBI CTAG 3 cut(s) 180, 207, 341
GlaI GCGC 1 cut(s) 860
GluI GCNGC 2 cut(s) 89, 961
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 862
HaeIII GGCC 2 cut(s) 776, 855
HapII CCGG 1 cut(s) 856
HhaI GCGC 1 cut(s) 861
Hin1II CATG 9 cut(s) 25, 178, 296, 315, 670, 677, 781, 790, 817
Hin6I GCGC 1 cut(s) 859
HinP1I GCGC 1 cut(s) 859
HinfI GANTC 4 cut(s) 70, 227, 404, 670
HpaII CCGG 1 cut(s) 856
HphI GGTGA 3 cut(s) 79, 888, 931
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 6 cut(s) 54, 224, 470, 533, 667, 949
Hpy8I GTNNAC 1 cut(s) 77
HpyAV CCTTC 3 cut(s) 25, 293, 476
HpyCH4III ACNGT 4 cut(s) 444, 503, 606, 825
HpyCH4IV ACGT 1 cut(s) 992
HpyCH4V TGCA 6 cut(s) 21, 95, 112, 652, 758, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 544, 969
HpyF3I CTNAG 2 cut(s) 837, 936
HpySE526I ACGT 1 cut(s) 992
Hsp92II CATG 9 cut(s) 25, 178, 296, 315, 670, 677, 781, 790, 817
HspAI GCGC 1 cut(s) 859
KpnI GGTACC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 855
KroNI GCCGGC 1 cut(s) 857
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 50, 170, 373, 394, 595, 883
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 3 cut(s) 200, 308, 535
Lsp1109I GCAGC 2 cut(s) 75, 972
MaeI CTAG 3 cut(s) 180, 207, 341
MaeII ACGT 1 cut(s) 992
MaeIII GTNAC 4 cut(s) 5, 415, 497, 993
MalI GATC 6 cut(s) 52, 172, 375, 396, 597, 885
MboI GATC 6 cut(s) 50, 170, 373, 394, 595, 883
MboII GAAGA 6 cut(s) 110, 212, 635, 653, 856, 970
MfeI CAATTG 2 cut(s) 149, 330
MflI RGATCY 2 cut(s) 50, 170
MhlI GDGCHC 2 cut(s) 540, 892
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 6 cut(s) 149, 186, 330, 552, 1005, 1017
MluNI TGGCCA 1 cut(s) 776
MnlI CCTC 8 cut(s) 57, 75, 153, 337, 466, 705, 915, 931
Mox20I TGGCCA 1 cut(s) 776
MroNI GCCGGC 1 cut(s) 855
MscI TGGCCA 1 cut(s) 776
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 2 cut(s) 584, 678
Msp20I TGGCCA 1 cut(s) 776
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 1 cut(s) 856
MunI CAATTG 2 cut(s) 149, 330
MwoI GCNNNNNNNGC 3 cut(s) 201, 544, 969
NaeI GCCGGC 1 cut(s) 857
NcoI CCATGG 1 cut(s) 777
NdeII GATC 6 cut(s) 50, 170, 373, 394, 595, 883
NgoMIV GCCGGC 1 cut(s) 855
NlaIII CATG 9 cut(s) 25, 178, 296, 315, 670, 677, 781, 790, 817
NlaIV GGNNCC 6 cut(s) 52, 172, 196, 246, 514, 807
NmuCI GTSAC 2 cut(s) 497, 993
NspI RCATGY 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 584
PagI TCATGA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 618
PdiI GCCGGC 1 cut(s) 857
PfeI GAWTC 4 cut(s) 70, 227, 404, 670
PkrI GCNGC 2 cut(s) 90, 962
PshBI ATTAAT 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 5
PspN4I GGNNCC 6 cut(s) 52, 172, 196, 246, 514, 807
PspPI GGNCC 1 cut(s) 806
PsuI RGATCY 2 cut(s) 50, 170
RsaI GTAC 4 cut(s) 246, 291, 323, 505
RsaNI GTAC 4 cut(s) 245, 290, 322, 504
RseI CAYNNNNRTG 2 cut(s) 584, 678
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 2 cut(s) 89, 961
Sau3AI GATC 6 cut(s) 50, 170, 373, 394, 595, 883
Sau96I GGNCC 1 cut(s) 806
ScaI AGTACT 1 cut(s) 505
SduI GDGCHC 2 cut(s) 540, 892
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 2 cut(s) 584, 678
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 149, 186, 330, 552, 1005, 1017
SsiI CCGC 2 cut(s) 545, 638
SspI AATATT 1 cut(s) 567
SspMI CTAG 3 cut(s) 180, 207, 341
StyI CCWWGG 1 cut(s) 777
TaaI ACNGT 4 cut(s) 444, 503, 606, 825
TaiI ACGT 1 cut(s) 995
TaqI TCGA 1 cut(s) 886
TasI AATT 6 cut(s) 149, 186, 330, 552, 1005, 1017
TatI WGTACW 2 cut(s) 289, 503
TfiI GAWTC 4 cut(s) 70, 227, 404, 670
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 3 cut(s) 807, 974, 1002
TseFI GTSAC 2 cut(s) 497, 993
TseI GCWGC 2 cut(s) 88, 960
Tsp45I GTSAC 2 cut(s) 497, 993
TspDTI ATGAA 7 cut(s) 128, 492, 559, 655, 662, 743, 856
TspRI CASTG 3 cut(s) 807, 974, 1002
VpaK11BI GGWCC 1 cut(s) 806
VspI ATTAAT 1 cut(s) 189
XagI CCTNNNNNAGG 2 cut(s) 470, 699
XapI RAATTY 2 cut(s) 552, 1005
XceI RCATGY 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XspI CTAG 3 cut(s) 180, 207, 341
ZrmI AGTACT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.