MD15G1403900.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013298
Physical Location & Seq
Reverse (-)
50473831 .. 50478228
4398 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1403900.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGGTAATCGATTTATTTGCATGTTAAAGAAGGACACCAAAGAAACTGGATCGAGAAGCAAGAGAATGAGTCGCTCGCAGAGGAAACCGGCGGCGGAGGAAGACTTGTTGCACAGGCAAGCTCTGTCTAGGGTTCTCCATCAGCACCAAATGTCTCAGAGGTTTGACGGATCCATGTCTAGGAGGGTTGGGGGGTCCACAAGCTCCAGGAGACTCGCTCTCTCTGATCCATTATCTAATGGGAAGCAGGGACCGGAATTTTTGGAGAAGCTTACATCAAAAAAGTTTATTCTTGTACATGGAGAAGGATTTGGGGCATGGTGTTGGTACAAAACAATTGCTTTACTAGAGGAAGCAGGATTGCTTCCAATCACCTTTGATCTCAAGGGTTCTGGTATTGATCTCGCAGATACGAACTCTATAACTACACTGGAAGAGTATTCAAAGCCATTGATTGACTATCTACAAAATCTTCCAGAGGATGAAAAGGTCATCTTAGTTGGTCACAGTAGTGGGGGTGCCTGCGTATCTTATGCAATGGAGCACTTCTCGCAGAAAATTTCAAAAGCAGTTTTCATCTGTGCTACTATGGTGTCTGATGGGCAGAGACCTTTTGATGTGTTTGCTGCAGAGCTTGGTTCCGCAGAACAATTTATGCAAGGATCAAAGTTTTTGATTCATGGGAATGGAAAAGAAAAGCCTCCTACAGGATTCATGTTTGAGAAAGAGCAGATGAAAGGGTTATACTTCAACCAATCTAATGCGAAGGATGTTGCTCTGGCCATGGTTTCCATGAGACCTATCCCACTAGGTCCGATCATGGAAAAACTGTCATTGACACCTGAAAATTATGGTTCTAGCCGGCGATTCTTCATTCAAACATTGGATGATCGGGCACTTTCACCAGACGTCCAAGAAAAGCTAGTGAGCGAAAACCCACCGGAGGGAGTGTACAAGATAAAAGGGAGTGACCACTGCCCGTTCTTCTCAAAGCCGCAATCATTGCACAAAATGTTGTTGGAAATTGCTCAAATTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

347

Amino Acids

38.62

Weight (kDa)

8.66

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 95 - 208 3.4e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 96 - 212 1.3e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 97 - 333 1e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013298)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33990 AT4G09900
fragaria_vesca FvH4_5g32490
malus_domestica MD08G1216700.v1.1 MD15G1403900.v1.1
prunus_persica Prupe.1G550200_v2.0.a1
pyrus_communis pycom08g18760 pycom15g36120
rosa_chinensis RchiOBHm_Chr7g0229071
rosa_laevigata RLG00000001533
rosa_multiflora Rmu_sc0000271.1_g000022
rosa_roxburghii Rroxscaffold_3G00230770
rosa_rugosa Rorug07G0255400
rosa_samantha Rh7AG406500 Rh7BG385900 Rh7CG424300 Rh7DG400800
rosa_wichuraiana Rw7G033740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 912
AccB1I GGYRCC 1 cut(s) 518
AciI CCGC 4 cut(s) 92, 95, 642, 995
AclWI GGATC 5 cut(s) 58, 165, 178, 221, 670
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 3 cut(s) 257, 558, 1032
AcyI GRCGYC 1 cut(s) 909
AfaI GTAC 3 cut(s) 297, 329, 953
AfiI CCNNNNNNNGG 4 cut(s) 180, 707, 943, 944
AgsI TTSAA 4 cut(s) 444, 564, 751, 878
AjnI CCWGG 1 cut(s) 206
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 5 cut(s) 122, 204, 271, 634, 922
AluI AGCT 5 cut(s) 122, 204, 271, 634, 922
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 4 cut(s) 159, 205, 601, 790
AlwI GGATC 5 cut(s) 58, 165, 178, 221, 670
AoxI GGCC 1 cut(s) 780
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 3 cut(s) 257, 558, 1032
AspS9I GGNCC 3 cut(s) 195, 251, 812
AsuHPI GGTGA 2 cut(s) 364, 894
AvaII GGWCC 3 cut(s) 195, 251, 812
BaeGI GKGCMC 1 cut(s) 898
BalI TGGCCA 1 cut(s) 782
BamHI GGATCC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 1 cut(s) 613
BccI CCATC 2 cut(s) 147, 593
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 208
BcoDI GTCTC 4 cut(s) 159, 205, 601, 790
BfaI CTAG 6 cut(s) 129, 180, 347, 809, 858, 923
BfmI CTRYAG 2 cut(s) 627, 705
BisI GCNGC 3 cut(s) 93, 627, 995
BlsI GCNGC 3 cut(s) 94, 628, 996
Bme1390I CCNGG 1 cut(s) 208
Bme18I GGWCC 3 cut(s) 195, 251, 812
BmgT120I GGNCC 3 cut(s) 195, 251, 812
BmiI GGNNCC 5 cut(s) 172, 196, 252, 520, 640
BmrFI CCNGG 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 4 cut(s) 202, 234, 533, 565
BpmI CTGGAG 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 368
Bsa29I ATCGAT 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 909
BsaI GGTCTC 2 cut(s) 601, 790
BsaJI CCNNGG 1 cut(s) 783
BsaWI WCCGGW 2 cut(s) 253, 940
BsaXI ACNNNNNCTCC 2 cut(s) 935, 965
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 707, 943, 944
Bse118I RCCGGY 2 cut(s) 88, 861
Bse1I ACTGG 2 cut(s) 52, 435
Bse3DI GCAATG 2 cut(s) 543, 1001
BseBI CCWGG 1 cut(s) 208
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 783
BseGI GGATG 3 cut(s) 487, 775, 892
BseLI CCNNNNNNNGG 4 cut(s) 180, 707, 943, 944
BseMI GCAATG 2 cut(s) 543, 1001
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 52, 435
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 1 cut(s) 613
BshFI GGCC 1 cut(s) 782
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 4 cut(s) 89, 254, 862, 941
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 4 cut(s) 180, 707, 943, 944
BsmAI GTCTC 4 cut(s) 159, 205, 601, 790
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 1 cut(s) 782
Bso31I GGTCTC 2 cut(s) 601, 790
Bsp1286I GDGCHC 2 cut(s) 546, 898
Bsp1407I TGTACA 2 cut(s) 295, 951
Bsp143I GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 4 cut(s) 92, 95, 642, 995
BspANI GGCC 1 cut(s) 782
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 10
BspLI GGNNCC 5 cut(s) 172, 196, 252, 520, 640
BspMAI CTGCAG 1 cut(s) 631
BspPI GGATC 5 cut(s) 58, 165, 178, 221, 670
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 2 cut(s) 601, 790
BsrDI GCAATG 2 cut(s) 543, 1001
BsrFI RCCGGY 2 cut(s) 88, 861
BsrGI TGTACA 2 cut(s) 295, 951
BsrI ACTGG 2 cut(s) 52, 435
BssAI RCCGGY 2 cut(s) 88, 861
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
BssNI GRCGYC 1 cut(s) 909
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 2 cut(s) 509, 831
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 1 cut(s) 909
BstAPI GCANNNNNTGC 1 cut(s) 1003
BstAUI TGTACA 2 cut(s) 295, 951
BstC8I GCNNGC 4 cut(s) 77, 120, 523, 863
BstDEI CTNAG 2 cut(s) 156, 496
BstDSI CCRYGG 1 cut(s) 783
BstENI CCTNNNNNAGG 1 cut(s) 705
BstF5I GGATG 3 cut(s) 487, 775, 892
BstKTI GATC 8 cut(s) 53, 173, 229, 382, 403, 665, 819, 892
BstMAI GTCTC 4 cut(s) 159, 205, 601, 790
BstMBI GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
BstMWI GCNNNNNNNGC 2 cut(s) 550, 1003
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 206
BstSFI CTRYAG 2 cut(s) 627, 705
BstSLI GKGCMC 1 cut(s) 898
BstV1I GCAGC 1 cut(s) 613
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 1 cut(s) 782
BsuTUI ATCGAT 1 cut(s) 10
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 3 cut(s) 487, 775, 892
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 2 cut(s) 428, 973
Cac8I GCNNGC 4 cut(s) 77, 120, 523, 863
Cfr10I RCCGGY 2 cut(s) 88, 861
Cfr13I GGNCC 3 cut(s) 195, 251, 812
ClaI ATCGAT 1 cut(s) 10
Csp6I GTAC 3 cut(s) 296, 328, 952
CviAII CATG 9 cut(s) 22, 175, 299, 318, 680, 715, 784, 793, 820
CviQI GTAC 3 cut(s) 296, 328, 952
DdeI CTNAG 2 cut(s) 156, 496
DpnI GATC 8 cut(s) 52, 172, 228, 381, 402, 664, 818, 891
DpnII GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
EaeI YGGCCR 1 cut(s) 780
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EciI GGCGGA 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 783
Eco31I GGTCTC 2 cut(s) 601, 790
Eco47I GGWCC 3 cut(s) 195, 251, 812
EcoNI CCTNNNNNAGG 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 9 cut(s) 25, 178, 302, 321, 683, 718, 787, 796, 823
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 1 cut(s) 264
FatI CATG 9 cut(s) 21, 174, 298, 317, 679, 714, 783, 792, 819
Fnu4HI GCNGC 3 cut(s) 93, 627, 995
FokI GGATG 3 cut(s) 494, 782, 899
Fsp4HI GCNGC 3 cut(s) 93, 627, 995
FspBI CTAG 6 cut(s) 129, 180, 347, 809, 858, 923
GluI GCNGC 3 cut(s) 93, 627, 995
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 1 cut(s) 782
HapII CCGG 4 cut(s) 89, 254, 862, 941
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 9 cut(s) 25, 178, 302, 321, 683, 718, 787, 796, 823
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 5 cut(s) 70, 213, 676, 711, 867
HpaII CCGG 4 cut(s) 89, 254, 862, 941
HphI GGTGA 2 cut(s) 364, 894
Hpy166II GTNNAC 2 cut(s) 198, 952
Hpy188I TCNGA 4 cut(s) 159, 226, 598, 816
Hpy188III TCNNGA 2 cut(s) 54, 476
Hpy8I GTNNAC 2 cut(s) 198, 952
HpyAV CCTTC 3 cut(s) 25, 299, 760
HpyCH4III ACNGT 2 cut(s) 509, 831
HpyCH4IV ACGT 1 cut(s) 909
HpyCH4V TGCA 6 cut(s) 21, 112, 536, 629, 658, 1006
HpyF10VI GCNNNNNNNGC 2 cut(s) 550, 1003
HpyF3I CTNAG 2 cut(s) 156, 496
HpySE526I ACGT 1 cut(s) 909
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 9 cut(s) 25, 178, 302, 321, 683, 718, 787, 796, 823
KroI GCCGGC 1 cut(s) 861
KroNI GCCGGC 1 cut(s) 863
Kzo9I GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
LmnI GCTCC 2 cut(s) 209, 541
Lsp1109I GCAGC 1 cut(s) 613
MaeI CTAG 6 cut(s) 129, 180, 347, 809, 858, 923
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 2 cut(s) 503, 968
MalI GATC 8 cut(s) 52, 172, 228, 381, 402, 664, 818, 891
MboI GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
MboII GAAGA 5 cut(s) 113, 446, 464, 862, 976
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 2 cut(s) 546, 898
MlsI TGGCCA 1 cut(s) 782
MluCI AATT 7 cut(s) 257, 336, 558, 650, 847, 1023, 1032
MluNI TGGCCA 1 cut(s) 782
MlyI GAGTC 2 cut(s) 79, 207
MmeI TCCRAC 1 cut(s) 999
MnlI CCTC 8 cut(s) 75, 91, 153, 177, 343, 472, 711, 937
Mox20I TGGCCA 1 cut(s) 782
MroNI GCCGGC 1 cut(s) 861
MscI TGGCCA 1 cut(s) 782
MseI TTAA 1 cut(s) 26
MslI CAYNNNNRTG 2 cut(s) 510, 684
Msp20I TGGCCA 1 cut(s) 782
MspI CCGG 4 cut(s) 89, 254, 862, 941
MspR9I CCNGG 1 cut(s) 208
MunI CAATTG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 550, 1003
NaeI GCCGGC 1 cut(s) 863
NcoI CCATGG 1 cut(s) 783
NdeII GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
NgoMIV GCCGGC 1 cut(s) 861
NlaIII CATG 9 cut(s) 25, 178, 302, 321, 683, 718, 787, 796, 823
NlaIV GGNNCC 5 cut(s) 172, 196, 252, 520, 640
NmuCI GTSAC 2 cut(s) 503, 968
NspI RCATGY 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 510
PdiI GCCGGC 1 cut(s) 863
PfeI GAWTC 3 cut(s) 676, 711, 867
PfoI TCCNGGA 1 cut(s) 206
PkrI GCNGC 3 cut(s) 94, 628, 996
PleI GAGTC 2 cut(s) 78, 207
PpsI GAGTC 2 cut(s) 78, 207
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 5 cut(s) 172, 196, 252, 520, 640
PspPI GGNCC 3 cut(s) 195, 251, 812
PstI CTGCAG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 3 cut(s) 297, 329, 953
RsaNI GTAC 3 cut(s) 296, 328, 952
RseI CAYNNNNRTG 2 cut(s) 510, 684
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 3 cut(s) 93, 627, 995
Sau3AI GATC 8 cut(s) 50, 170, 226, 379, 400, 662, 816, 889
Sau96I GGNCC 3 cut(s) 195, 251, 812
SchI GAGTC 2 cut(s) 79, 207
ScrFI CCNGG 1 cut(s) 208
SduI GDGCHC 2 cut(s) 546, 898
SfcI CTRYAG 2 cut(s) 627, 705
SinI GGWCC 3 cut(s) 195, 251, 812
SmiMI CAYNNNNRTG 2 cut(s) 510, 684
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 7 cut(s) 257, 336, 558, 650, 847, 1023, 1032
SsiI CCGC 4 cut(s) 92, 95, 642, 995
SspMI CTAG 6 cut(s) 129, 180, 347, 809, 858, 923
StyD4I CCNGG 1 cut(s) 206
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 2 cut(s) 509, 831
TaiI ACGT 1 cut(s) 912
TaqI TCGA 2 cut(s) 10, 53
TasI AATT 7 cut(s) 257, 336, 558, 650, 847, 1023, 1032
TatI WGTACW 2 cut(s) 295, 951
TauI GCSGC 2 cut(s) 95, 997
TfiI GAWTC 3 cut(s) 676, 711, 867
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TscAI CASTG 2 cut(s) 435, 980
TseFI GTSAC 2 cut(s) 503, 968
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 2 cut(s) 503, 968
TspDTI ATGAA 6 cut(s) 498, 565, 668, 703, 749, 862
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 435, 980
VpaK11BI GGWCC 3 cut(s) 195, 251, 812
XagI CCTNNNNNAGG 1 cut(s) 705
XapI RAATTY 3 cut(s) 257, 558, 1032
XceI RCATGY 1 cut(s) 25
XspI CTAG 6 cut(s) 129, 180, 347, 809, 858, 923
ZraI GACGTC 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.