AT1G34750

protein phosphatase 2C 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004594
Physical Location & Seq
Reverse (-)
12736000 .. 12738196
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34750.4

Sequence Viewer

Length: 849 bp
ATGGCGAAGTTATGTTGCTTCGGTTCTTCAGACTACGATCTTGTGGTTGGGAGGGCATCGACTAGTTCTGGAAAAGGGAGAAATAACGACGGCGAGATTAAATTCGGTTACAGCTTAGTGAAAGGGAAAGCTAATCATCCAATGGAGGATTATCATGTGTCTAAATTCGTGAAAATAGACGGTAACGAGCTCGGTTTGTTTGCAATCTACGATGGTCATTTGGGTGAACGTGTTCCTGCTTATCTACAGAAGCATTTGTTCTCCAATATCCTCAAAGAGGAGCAGTTTAGGTATGATCCTCAGAGATCAATAATTGCTGCCTATGAGAAGACAGACCAAGCCATTCTGTCACATTCTTCTGACTTAGGCCGTGGTGGCTCAACGGCTGTAACCGCTATTCTGATGAATGGACGGCGTTTATGGGTGGCAAATGTGGGTGATTCTCGGGCGGTTCTATCTCAAGGAGGTCAGGCAATACAGATGACTATTGATCATGAGCCGCACACTGAAAGGTTGAGCATCGAGGGTAAAGGAGGCTTTGTATCCAACATGCCAGGAGATGTCCCTCGGGTTAACGGGCAGCTAGCGGTTTCTCGTGCTTTTGGAGACAAAAGCCTCAAAACACATCTACGGTCAGATCCTGACGTTAAAGATTCATCGATCGATGATCACACAGATGTTCTTGTTCTTGCTAGTGACGGTCTATGGAAGGTGATGGCTAACCAAGAGGCGATTGATATTGCGAGAAGAATCAAAGATCCATTGAAAGCAGCGAAAGAACTAACAACCGAAGCGCTTAGAAGAGACAGTAAAGATGATATTTCTTGCATTGTCGTCAGATTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

282

Amino Acids

30.98

Weight (kDa)

7.13

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 44 - 241 2.1e-43 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 393, 449, 500, 587
AclWI GGATC 3 cut(s) 290, 632, 752
AcsI RAATTY 2 cut(s) 101, 164
AcuI CTGAAG 1 cut(s) 12
AfeI AGCGCT 1 cut(s) 795
AfiI CCNNNNNNNGG 1 cut(s) 277
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 1 cut(s) 766
AhlI ACTAGT 1 cut(s) 62
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 4 cut(s) 114, 131, 190, 583
AluI AGCT 4 cut(s) 114, 131, 190, 583
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 600, 798
AlwI GGATC 3 cut(s) 290, 632, 752
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 2 cut(s) 444, 567
Aor51HI AGCGCT 1 cut(s) 795
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 3 cut(s) 317, 580, 770
ApoI RAATTY 2 cut(s) 101, 164
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 796
AsuHPI GGTGA 3 cut(s) 236, 449, 724
AsuNHI GCTAGC 1 cut(s) 583
AvaI CYCGRG 2 cut(s) 444, 567
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 304, 592, 782
BccI CCATC 2 cut(s) 206, 709
BceAI ACGGC 4 cut(s) 106, 354, 399, 428
BciT130I CCWGG 1 cut(s) 555
BciVI GTATCC 1 cut(s) 553
BclI TGATCA 2 cut(s) 490, 667
BcoDI GTCTC 2 cut(s) 600, 798
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 3 cut(s) 63, 584, 693
BfmI CTRYAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 797
BfuI GTATCC 1 cut(s) 553
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 4 cut(s) 318, 500, 581, 771
BlsI GCNGC 4 cut(s) 319, 501, 582, 772
Bme1390I CCNGG 1 cut(s) 555
BmeT110I CYCGRG 2 cut(s) 444, 567
BmrFI CCNGG 1 cut(s) 555
BmsI GCATC 2 cut(s) 65, 528
BmtI GCTAGC 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 444
Bsa29I ATCGAT 2 cut(s) 659, 663
BsaJI CCNNGG 2 cut(s) 370, 566
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 555
BseCI ATCGAT 2 cut(s) 659, 663
BseDI CCNNGG 2 cut(s) 370, 566
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 3 cut(s) 304, 592, 782
Bsh1285I CGRYCG 1 cut(s) 663
BshFI GGCC 1 cut(s) 369
BshVI ATCGAT 2 cut(s) 659, 663
BsiEI CGRYCG 1 cut(s) 663
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 2 cut(s) 444, 567
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 2 cut(s) 600, 798
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 1 cut(s) 369
BsoBI CYCGRG 2 cut(s) 444, 567
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
BspACI CCGC 4 cut(s) 393, 449, 500, 587
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 313
BspDI ATCGAT 2 cut(s) 659, 663
BspHI TCATGA 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 587
BspPI GGATC 3 cut(s) 290, 632, 752
BssECI CCNNGG 2 cut(s) 370, 566
BssMI GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
BssSI CACGAG 1 cut(s) 594
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 182, 633, 701, 809
Bst6I CTCTTC 1 cut(s) 796
BstC8I GCNNGC 1 cut(s) 585
BstDEI CTNAG 4 cut(s) 115, 300, 364, 797
BstDSI CCRYGG 1 cut(s) 370
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 797
BstHHI GCGC 1 cut(s) 796
BstKTI GATC 8 cut(s) 40, 298, 308, 493, 640, 663, 670, 760
BstMAI GTCTC 2 cut(s) 600, 798
BstMBI GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
BstMCI CGRYCG 1 cut(s) 663
BstMWI GCNNNNNNNGC 2 cut(s) 375, 392
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 3 cut(s) 304, 592, 782
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 637, 757
BstYI RGATCY 2 cut(s) 637, 757
Bsu15I ATCGAT 2 cut(s) 659, 663
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 1 cut(s) 369
BsuTUI ATCGAT 2 cut(s) 659, 663
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 585
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 493
CfoI GCGC 1 cut(s) 796
ClaI ATCGAT 2 cut(s) 659, 663
CviAII CATG 3 cut(s) 155, 494, 550
DdeI CTNAG 4 cut(s) 115, 300, 364, 797
DpnI GATC 8 cut(s) 39, 297, 307, 492, 639, 662, 669, 759
DpnII GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
Eam1104I CTCTTC 1 cut(s) 796
EarI CTCTTC 1 cut(s) 796
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco47III AGCGCT 1 cut(s) 795
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 2 cut(s) 444, 567
EcoICRI GAGCTC 1 cut(s) 190
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 3 cut(s) 158, 497, 553
FaiI YATR 8 cut(s) 13, 156, 294, 324, 421, 495, 551, 706
FaqI GGGAC 1 cut(s) 548
FatI CATG 3 cut(s) 154, 493, 549
FbaI TGATCA 2 cut(s) 490, 667
Fnu4HI GCNGC 4 cut(s) 318, 500, 581, 771
FokI GGATG 1 cut(s) 123
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 318, 500, 581, 771
FspBI CTAG 3 cut(s) 63, 584, 693
GlaI GCGC 1 cut(s) 795
GluI GCNGC 4 cut(s) 318, 500, 581, 771
HaeII RGCGCY 1 cut(s) 797
HaeIII GGCC 1 cut(s) 369
HhaI GCGC 1 cut(s) 796
Hin1II CATG 3 cut(s) 158, 497, 553
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 3 cut(s) 440, 653, 750
HpaI GTTAAC 1 cut(s) 574
HphI GGTGA 3 cut(s) 236, 449, 724
Hpy166II GTNNAC 2 cut(s) 227, 574
Hpy188I TCNGA 6 cut(s) 31, 303, 361, 402, 637, 839
Hpy188III TCNNGA 4 cut(s) 69, 169, 494, 641
Hpy8I GTNNAC 2 cut(s) 227, 574
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 703
HpyCH4III ACNGT 4 cut(s) 182, 633, 701, 809
HpyCH4IV ACGT 2 cut(s) 229, 645
HpyCH4V TGCA 2 cut(s) 203, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 392
HpyF3I CTNAG 4 cut(s) 115, 300, 364, 797
HpySE526I ACGT 2 cut(s) 229, 645
Hsp92II CATG 3 cut(s) 158, 497, 553
HspAI GCGC 1 cut(s) 794
Ksp22I TGATCA 2 cut(s) 490, 667
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 6 cut(s) 54, 249, 455, 540, 567, 654
Lsp1109I GCAGC 3 cut(s) 304, 592, 782
LweI GCATC 2 cut(s) 65, 528
MaeI CTAG 3 cut(s) 63, 584, 693
MaeII ACGT 2 cut(s) 229, 645
MaeIII GTNAC 5 cut(s) 107, 182, 348, 388, 695
MalI GATC 8 cut(s) 39, 297, 307, 492, 639, 662, 669, 759
MboI GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
MboII GAAGA 5 cut(s) 18, 340, 348, 759, 813
MflI RGATCY 2 cut(s) 637, 757
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 3 cut(s) 101, 164, 312
MmeI TCCRAC 1 cut(s) 570
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 4 cut(s) 99, 573, 648, 842
MslI CAYNNNNRTG 2 cut(s) 222, 675
MspR9I CCNGG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 2 cut(s) 375, 392
NdeII GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
NheI GCTAGC 1 cut(s) 583
NlaIII CATG 3 cut(s) 158, 497, 553
NmuCI GTSAC 2 cut(s) 348, 695
NspI RCATGY 1 cut(s) 553
PagI TCATGA 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 440, 653, 750
PkrI GCNGC 4 cut(s) 319, 501, 582, 772
Ple19I CGATCG 1 cut(s) 663
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 637, 757
PvuI CGATCG 1 cut(s) 663
RseI CAYNNNNRTG 2 cut(s) 222, 675
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 4 cut(s) 99, 573, 648, 842
SatI GCNGC 4 cut(s) 318, 500, 581, 771
Sau3AI GATC 8 cut(s) 37, 295, 305, 490, 637, 660, 667, 757
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 2 cut(s) 65, 528
SfcI CTRYAG 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 222, 675
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 62
Sse9I AATT 3 cut(s) 101, 164, 312
SsiI CCGC 4 cut(s) 393, 449, 500, 587
SspMI CTAG 3 cut(s) 63, 584, 693
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 4 cut(s) 182, 633, 701, 809
TaiI ACGT 2 cut(s) 232, 648
TaqI TCGA 4 cut(s) 59, 522, 659, 663
TasI AATT 3 cut(s) 101, 164, 312
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 3 cut(s) 440, 653, 750
Tru1I TTAA 4 cut(s) 99, 573, 648, 842
Tru9I TTAA 4 cut(s) 99, 573, 648, 842
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 2 cut(s) 348, 695
TseI GCWGC 3 cut(s) 317, 580, 770
Tsp45I GTSAC 2 cut(s) 348, 695
TspDTI ATGAA 2 cut(s) 419, 645
TspRI CASTG 1 cut(s) 511
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 2 cut(s) 101, 164
XceI RCATGY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 3 cut(s) 63, 584, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.