Rroxscaffold_3G00221560

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004594
Physical Location & Seq
Forward (+)
4321010 .. 4326675
5666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221560.1

Sequence Viewer

Length: 711 bp
ATGGAGGATTATCATGTTGCTAAGTTCGTTAGGTCCCAGGGACATGAGTTAGGATTGTTTGCTATATATGATGGCCATTTGGGAGACACCGTGCCTCAATACTTACAGAAGCATTTGTTTTCAAATATTCTTAAGGAGGAAGAATTTTGGGTCGACCCTAACAGATCTATCTCAAAAGCCTATGAGAGGACAGACCAGGCAATTCTCTCGCATAGCACTGACTTGGGGCGTGGCGGGTCCACTGCTGTCACTGCAATATTGTTAAATGGTCAGCGGTTATATGTGGCCAACGTTGGAGATTCGCGGGCGGTTCTTTCAAAGGAGGGTCGGGCAGTACAGATGTCAATAGACCATGAACCCAATACTGAACGAGGCAGCATTGAAAACAAAGGTGGCTTTGTCTCAAACATGCCAGGAGATGTACCAAGAGTTAATGGGCAGCTGGCAGTTTCTCGTGCTTTTGGAGACAAAAGCCTCAAGTCGCATTTACGATCAGACCCAGACATACAGGATACTTACATAGACATGAATACAGATGTTCTAATCCTTGCCAGTGATGGCCTTTGGAAGGTCATGGGTAATCAAGAGGCAGTTGATATTGCAAGAAAAATTAAAGACCCTATGAAGGCAGCTAAGGAGTTAACAGCTGAAGCTTTGAAAAGAGATAGTAAAGATGATATATCTTGCGTTGTTGTTAGATTTAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

236

Amino Acids

26.13

Weight (kDa)

5.99

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 1 - 227 4.6e-56 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccII CGCG 1 cut(s) 304
AciI CCGC 4 cut(s) 234, 274, 304, 308
AclI AACGTT 1 cut(s) 291
AcoI YGGCCR 2 cut(s) 73, 285
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 2 cut(s) 336, 423
AfiI CCNNNNNNNGG 3 cut(s) 186, 568, 625
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 123, 318, 383, 658
AjnI CCWGG 3 cut(s) 36, 195, 412
AluBI AGCT 4 cut(s) 442, 632, 647, 653
AluI AGCT 4 cut(s) 442, 632, 647, 653
Alw26I GTCTC 3 cut(s) 78, 406, 459
AoxI GGCC 3 cut(s) 73, 285, 559
ApeKI GCWGC 3 cut(s) 375, 439, 629
ApoI RAATTY 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 33, 237
AvaII GGWCC 2 cut(s) 33, 237
BalI TGGCCA 2 cut(s) 75, 287
BauI CACGAG 1 cut(s) 453
BbvI GCAGC 3 cut(s) 387, 451, 641
BccI CCATC 2 cut(s) 65, 551
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BciT130I CCWGG 3 cut(s) 38, 197, 414
BciVI GTATCC 1 cut(s) 505
BcoDI GTCTC 3 cut(s) 78, 406, 459
BfrI CTTAAG 1 cut(s) 131
BfuI GTATCC 1 cut(s) 505
BglII AGATCT 1 cut(s) 164
BisI GCNGC 3 cut(s) 376, 440, 630
BlsI GCNGC 3 cut(s) 377, 441, 631
Bme1390I CCNGG 3 cut(s) 38, 197, 414
Bme18I GGWCC 2 cut(s) 33, 237
BmgT120I GGNCC 2 cut(s) 33, 237
BmiI GGNNCC 2 cut(s) 35, 238
BmrFI CCNGG 3 cut(s) 38, 197, 414
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 36, 37
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 568, 625
Bse1I ACTGG 1 cut(s) 552
BseBI CCWGG 3 cut(s) 38, 197, 414
BseDI CCNNGG 2 cut(s) 36, 37
BseLI CCNNNNNNNGG 3 cut(s) 186, 568, 625
BseNI ACTGG 1 cut(s) 552
BseXI GCAGC 3 cut(s) 387, 451, 641
Bsh1236I CGCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 75, 287, 561
BslFI GGGAC 2 cut(s) 19, 54
BslI CCNNNNNNNGG 3 cut(s) 186, 568, 625
BsmAI GTCTC 3 cut(s) 78, 406, 459
BsmFI GGGAC 2 cut(s) 19, 54
BsnI GGCC 3 cut(s) 75, 287, 561
Bsp143I GATC 2 cut(s) 164, 491
BspACI CCGC 4 cut(s) 234, 274, 304, 308
BspANI GGCC 3 cut(s) 75, 287, 561
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 35, 238
BspTI CTTAAG 1 cut(s) 131
BsrI ACTGG 1 cut(s) 552
BssECI CCNNGG 2 cut(s) 36, 37
BssMI GATC 2 cut(s) 164, 491
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 38, 197, 414
Bst4CI ACNGT 1 cut(s) 91
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 306, 444
BstDEI CTNAG 2 cut(s) 21, 633
BstENI CCTNNNNNAGG 2 cut(s) 184, 566
BstFNI CGCG 1 cut(s) 304
BstKTI GATC 2 cut(s) 167, 494
BstMAI GTCTC 3 cut(s) 78, 406, 459
BstMBI GATC 2 cut(s) 164, 491
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 3 cut(s) 38, 197, 414
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 3 cut(s) 36, 195, 412
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 3 cut(s) 387, 451, 641
BstX2I RGATCY 1 cut(s) 164
BstYI RGATCY 1 cut(s) 164
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 3 cut(s) 75, 287, 561
BtsI GCAGTG 2 cut(s) 240, 249
BtsIMutI CAGTG 4 cut(s) 216, 240, 249, 559
Cac8I GCNNGC 2 cut(s) 306, 444
Cfr13I GGNCC 2 cut(s) 33, 237
Csp6I GTAC 2 cut(s) 335, 422
CviAII CATG 6 cut(s) 14, 44, 353, 409, 526, 574
CviQI GTAC 2 cut(s) 335, 422
DdeI CTNAG 2 cut(s) 21, 633
DpnI GATC 2 cut(s) 166, 493
DpnII GATC 2 cut(s) 164, 491
EaeI YGGCCR 2 cut(s) 73, 285
Eco47I GGWCC 2 cut(s) 33, 237
Eco57I CTGAAG 1 cut(s) 669
EcoNI CCTNNNNNAGG 2 cut(s) 184, 566
EcoO109I RGGNCCY 1 cut(s) 33
EcoRII CCWGG 3 cut(s) 36, 195, 412
FaeI CATG 6 cut(s) 17, 47, 356, 412, 529, 577
FaqI GGGAC 2 cut(s) 19, 54
FatI CATG 6 cut(s) 13, 43, 352, 408, 525, 573
FauI CCCGC 2 cut(s) 227, 297
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 376, 440, 630
Fsp4HI GCNGC 3 cut(s) 376, 440, 630
GluI GCNGC 3 cut(s) 376, 440, 630
HaeIII GGCC 3 cut(s) 75, 287, 561
Hin1II CATG 6 cut(s) 17, 47, 356, 412, 529, 577
HincII GTYRAC 2 cut(s) 154, 642
HindII GTYRAC 2 cut(s) 154, 642
HindIII AAGCTT 1 cut(s) 651
HinfI GANTC 1 cut(s) 299
HpaI GTTAAC 1 cut(s) 642
Hpy166II GTNNAC 3 cut(s) 154, 240, 642
Hpy188I TCNGA 1 cut(s) 496
Hpy188III TCNNGA 1 cut(s) 584
Hpy8I GTNNAC 3 cut(s) 154, 240, 642
HpyAV CCTTC 2 cut(s) 562, 619
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 2 cut(s) 254, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 21, 633
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 6 cut(s) 17, 47, 356, 412, 529, 577
KspAI GTTAAC 1 cut(s) 642
Kzo9I GATC 2 cut(s) 164, 491
Lsp1109I GCAGC 3 cut(s) 387, 451, 641
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 166, 493
MboI GATC 2 cut(s) 164, 491
MboII GAAGA 1 cut(s) 152
MflI RGATCY 1 cut(s) 164
MlsI TGGCCA 2 cut(s) 75, 287
MluCI AATT 3 cut(s) 143, 201, 609
MluNI TGGCCA 2 cut(s) 75, 287
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 7 cut(s) 105, 130, 180, 316, 365, 485, 580
Mox20I TGGCCA 2 cut(s) 75, 287
MscI TGGCCA 2 cut(s) 75, 287
MseI TTAA 5 cut(s) 132, 263, 432, 612, 641
MslI CAYNNNNRTG 1 cut(s) 524
Msp20I TGGCCA 2 cut(s) 75, 287
MspA1I CMGCKG 3 cut(s) 274, 442, 647
MspCI CTTAAG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 38, 197, 414
MvaI CCWGG 3 cut(s) 38, 197, 414
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 251
NdeII GATC 2 cut(s) 164, 491
NlaIII CATG 6 cut(s) 17, 47, 356, 412, 529, 577
NlaIV GGNNCC 2 cut(s) 35, 238
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 412
PasI CCCWGGG 1 cut(s) 37
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 3 cut(s) 377, 441, 631
PpuMI RGGWCCY 1 cut(s) 33
Psp1406I AACGTT 1 cut(s) 291
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 3 cut(s) 36, 195, 412
PspGI CCWGG 3 cut(s) 36, 195, 412
PspN4I GGNNCC 2 cut(s) 35, 238
PspPI GGNCC 2 cut(s) 33, 237
PspPPI RGGWCCY 1 cut(s) 33
PsuI RGATCY 1 cut(s) 164
PvuII CAGCTG 2 cut(s) 442, 647
RsaI GTAC 2 cut(s) 336, 423
RsaNI GTAC 2 cut(s) 335, 422
RseI CAYNNNNRTG 1 cut(s) 524
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 5 cut(s) 132, 263, 432, 612, 641
SatI GCNGC 3 cut(s) 376, 440, 630
Sau3AI GATC 2 cut(s) 164, 491
Sau96I GGNCC 2 cut(s) 33, 237
ScrFI CCNGG 3 cut(s) 38, 197, 414
SetI ASST 8 cut(s) 35, 294, 394, 444, 573, 634, 649, 655
SinI GGWCC 2 cut(s) 33, 237
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 131, 476
SmoI CTYRAG 2 cut(s) 131, 476
Sse9I AATT 3 cut(s) 143, 201, 609
SsiI CCGC 4 cut(s) 234, 274, 304, 308
SspI AATATT 2 cut(s) 127, 258
StyD4I CCNGG 3 cut(s) 36, 195, 412
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 153
TasI AATT 3 cut(s) 143, 201, 609
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 5 cut(s) 132, 263, 432, 612, 641
Tru9I TTAA 5 cut(s) 132, 263, 432, 612, 641
TscAI CASTG 4 cut(s) 223, 247, 256, 559
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 3 cut(s) 375, 439, 629
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 3 cut(s) 369, 542, 638
TspRI CASTG 4 cut(s) 223, 247, 256, 559
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 33, 237
XagI CCTNNNNNAGG 2 cut(s) 184, 566
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.