MD08G1183600.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004594
Physical Location & Seq
Forward (+)
22876089 .. 22880992
4904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1183600.v1.1.491

Sequence Viewer

Length: 777 bp
ATGTTAATTTTTCTGTGCAGTATGGGGTATGGCTTCAGCCTGGTAAAAGGAAAAGCAAATCATCCAATGGAGGATTATCATGTTGCTAAGTTTGTTCAGATCCAGGGACATGAGTTAGGATTGTTTGCTATTTATGATGGCCATTTGGGAGATAATGTGCCTGCATACCTACAGAAGCATTTATTTTCAAATATCCTTAAGGAGGAAGAGTTTTGGGTTGATCCAAACAGATCTATCTCAAAAGCCTATGAGAGGACAGACCAGGCAATTCTCTCGCATAGTTCTGACTTGGGCCGTGGCGGGTCTACTGCTGTTACTGCAATATTGTTAAACGGTCGGCGGTTATTGGTTGCAAATGTTGGAGATTCACGGGCAGTTCTTTCAAGGGCGGGTCAGGCAGTACAGATGTCTATAGATCATGAGCCCAACAACGAACGAGGCAGCATTGAAAACAAAGGTGGCTTTGTCTCTAACATGCCAGGAGATGTCCCTAGAGTCAATGGGCAGCTAGCAGTTTCTCGTGCCTTTGGAGACAAAAGCCTCAAGTCACATTTGCGATCGGATCCCGACATACAAGATGTCTACGTAGACAATAGTACTGATGTTCTAATCCTTGCCAGTGATGGTCTTTGGAAGGTCATGGAGAATCAAGAGGTGGTTAATATGGCAAGAAGGACTAAAGACCCTCTGAAGGCGGCAAAGCAATTAACCGCCGAAGCACTGAAAAGAGACAGTAAAGATGATATTTCATGCGTTGTTGTTAGACTAAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

259

Amino Acids

28.42

Weight (kDa)

6.71

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 18 - 249 2.9e-55 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 305, 582, 588
AciI CCGC 5 cut(s) 300, 340, 389, 695, 711
AclWI GGATC 4 cut(s) 94, 215, 557, 570
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 19, 710
AfaI GTAC 2 cut(s) 402, 598
AfiI CCNNNNNNNGG 3 cut(s) 202, 252, 691
AflII CTTAAG 1 cut(s) 197
AgsI TTSAA 3 cut(s) 189, 384, 449
AjnI CCWGG 4 cut(s) 39, 102, 261, 478
AluBI AGCT 1 cut(s) 508
AluI AGCT 1 cut(s) 508
Alw26I GTCTC 3 cut(s) 472, 525, 723
AlwI GGATC 4 cut(s) 94, 215, 557, 570
AoxI GGCC 2 cut(s) 139, 292
ApeKI GCWGC 2 cut(s) 441, 505
AspS9I GGNCC 1 cut(s) 292
AsuNHI GCTAGC 1 cut(s) 508
BalI TGGCCA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 426
BauI CACGAG 1 cut(s) 519
BbvI GCAGC 2 cut(s) 453, 517
BccI CCATC 2 cut(s) 131, 617
BceAI ACGGC 1 cut(s) 279
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BciT130I CCWGG 4 cut(s) 41, 104, 263, 480
BcoDI GTCTC 3 cut(s) 472, 525, 723
BfaI CTAG 2 cut(s) 492, 509
BfmI CTRYAG 2 cut(s) 170, 411
BfrI CTTAAG 1 cut(s) 197
BglII AGATCT 1 cut(s) 230
BisI GCNGC 3 cut(s) 442, 506, 696
BlsI GCNGC 3 cut(s) 443, 507, 697
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 4 cut(s) 41, 104, 263, 480
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 4 cut(s) 41, 104, 263, 480
BmtI GCTAGC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 527
BsaAI YACGTR 1 cut(s) 586
BsaJI CCNNGG 2 cut(s) 103, 295
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 194, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 252, 691
Bse1I ACTGG 1 cut(s) 618
BseBI CCWGG 4 cut(s) 41, 104, 263, 480
BseDI CCNNGG 2 cut(s) 103, 295
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 3 cut(s) 202, 252, 691
BseNI ACTGG 1 cut(s) 618
BseXI GCAGC 2 cut(s) 453, 517
BsgI GTGCAG 1 cut(s) 37
Bsh1285I CGRYCG 2 cut(s) 337, 560
BshFI GGCC 2 cut(s) 141, 294
BsiEI CGRYCG 2 cut(s) 337, 560
BslFI GGGAC 2 cut(s) 120, 473
BslI CCNNNNNNNGG 3 cut(s) 202, 252, 691
BsmAI GTCTC 3 cut(s) 472, 525, 723
BsmFI GGGAC 2 cut(s) 120, 473
BsnI GGCC 2 cut(s) 141, 294
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 6 cut(s) 99, 220, 230, 415, 557, 562
BspACI CCGC 5 cut(s) 300, 340, 389, 695, 711
BspANI GGCC 2 cut(s) 141, 294
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 1 cut(s) 564
BspOI GCTAGC 1 cut(s) 512
BspPI GGATC 4 cut(s) 94, 215, 557, 570
BspTI CTTAAG 1 cut(s) 197
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 2 cut(s) 103, 295
BssMI GATC 6 cut(s) 99, 220, 230, 415, 557, 562
BssSI CACGAG 1 cut(s) 519
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 4 cut(s) 41, 104, 263, 480
Bst4CI ACNGT 2 cut(s) 335, 734
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 197
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 162, 510
BstDEI CTNAG 2 cut(s) 87, 767
BstDSI CCRYGG 1 cut(s) 295
BstENI CCTNNNNNAGG 2 cut(s) 200, 250
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 6 cut(s) 102, 223, 233, 418, 560, 565
BstMAI GTCTC 3 cut(s) 472, 525, 723
BstMBI GATC 6 cut(s) 99, 220, 230, 415, 557, 562
BstMCI CGRYCG 2 cut(s) 337, 560
BstMWI GCNNNNNNNGC 2 cut(s) 317, 395
BstNI CCWGG 4 cut(s) 41, 104, 263, 480
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 4 cut(s) 39, 102, 261, 478
BstSFI CTRYAG 2 cut(s) 170, 411
BstSNI TACGTA 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 453, 517
BstX2I RGATCY 3 cut(s) 99, 230, 562
BstYI RGATCY 3 cut(s) 99, 230, 562
BsuRI GGCC 2 cut(s) 141, 294
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 625, 719
Cac8I GCNNGC 2 cut(s) 162, 510
CciI TCATGA 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 401, 597
CviAII CATG 6 cut(s) 80, 110, 419, 475, 640, 750
CviJI RGCY 9 cut(s) 33, 39, 141, 245, 294, 424, 462, 508, 540
CviKI_1 RGCY 9 cut(s) 33, 39, 141, 245, 294, 424, 462, 508, 540
CviQI GTAC 2 cut(s) 401, 597
DdeI CTNAG 2 cut(s) 87, 767
DpnI GATC 6 cut(s) 101, 222, 232, 417, 559, 564
DpnII GATC 6 cut(s) 99, 220, 230, 415, 557, 562
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco105I TACGTA 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 426
Eco57I CTGAAG 2 cut(s) 19, 710
EcoNI CCTNNNNNAGG 2 cut(s) 200, 250
EcoRII CCWGG 4 cut(s) 39, 102, 261, 478
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 6 cut(s) 83, 113, 422, 478, 643, 753
FaqI GGGAC 2 cut(s) 120, 473
FatI CATG 6 cut(s) 79, 109, 418, 474, 639, 749
FauI CCCGC 2 cut(s) 293, 382
FblI GTMKAC 3 cut(s) 305, 582, 588
Fnu4HI GCNGC 3 cut(s) 442, 506, 696
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 3 cut(s) 442, 506, 696
FspBI CTAG 2 cut(s) 492, 509
GluI GCNGC 3 cut(s) 442, 506, 696
HaeIII GGCC 2 cut(s) 141, 294
Hin1II CATG 6 cut(s) 83, 113, 422, 478, 643, 753
HinfI GANTC 3 cut(s) 365, 495, 646
Hpy166II GTNNAC 3 cut(s) 306, 583, 589
Hpy188I TCNGA 4 cut(s) 99, 286, 562, 690
Hpy188III TCNNGA 3 cut(s) 419, 566, 650
Hpy8I GTNNAC 3 cut(s) 306, 583, 589
HpyAV CCTTC 3 cut(s) 628, 666, 685
HpyCH4III ACNGT 2 cut(s) 335, 734
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 4 cut(s) 18, 164, 320, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 395
HpyF3I CTNAG 2 cut(s) 87, 767
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 6 cut(s) 83, 113, 422, 478, 643, 753
Kzo9I GATC 6 cut(s) 99, 220, 230, 415, 557, 562
Lsp1109I GCAGC 2 cut(s) 453, 517
MaeI CTAG 2 cut(s) 492, 509
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 313, 546
MalI GATC 6 cut(s) 101, 222, 232, 417, 559, 564
MboI GATC 6 cut(s) 99, 220, 230, 415, 557, 562
MboII GAAGA 1 cut(s) 218
MflI RGATCY 3 cut(s) 99, 230, 562
MhlI GDGCHC 1 cut(s) 426
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 6, 267, 704
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 7 cut(s) 64, 196, 246, 431, 551, 646, 696
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 5 cut(s) 5, 198, 329, 660, 707
Msp20I TGGCCA 1 cut(s) 141
MspCI CTTAAG 1 cut(s) 197
MspR9I CCNGG 4 cut(s) 41, 104, 263, 480
MvaI CCWGG 4 cut(s) 41, 104, 263, 480
MwoI GCNNNNNNNGC 2 cut(s) 317, 395
NdeII GATC 6 cut(s) 99, 220, 230, 415, 557, 562
NheI GCTAGC 1 cut(s) 508
NlaIII CATG 6 cut(s) 83, 113, 422, 478, 643, 753
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 1 cut(s) 478
PagI TCATGA 1 cut(s) 418
PfeI GAWTC 2 cut(s) 365, 646
PkrI GCNGC 3 cut(s) 443, 507, 697
Ple19I CGATCG 1 cut(s) 560
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Ppu21I YACGTR 1 cut(s) 586
Psp6I CCWGG 4 cut(s) 39, 102, 261, 478
PspGI CCWGG 4 cut(s) 39, 102, 261, 478
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 1 cut(s) 292
PsuI RGATCY 3 cut(s) 99, 230, 562
PvuI CGATCG 1 cut(s) 560
RsaI GTAC 2 cut(s) 402, 598
RsaNI GTAC 2 cut(s) 401, 597
SaqAI TTAA 5 cut(s) 5, 198, 329, 660, 707
SatI GCNGC 3 cut(s) 442, 506, 696
Sau3AI GATC 6 cut(s) 99, 220, 230, 415, 557, 562
Sau96I GGNCC 1 cut(s) 292
ScaI AGTACT 1 cut(s) 598
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 4 cut(s) 41, 104, 263, 480
SduI GDGCHC 1 cut(s) 426
SetI ASST 6 cut(s) 171, 460, 510, 588, 639, 657
SfcI CTRYAG 2 cut(s) 170, 411
SmlI CTYRAG 2 cut(s) 197, 542
SmoI CTYRAG 2 cut(s) 197, 542
SnaBI TACGTA 1 cut(s) 586
Sse9I AATT 3 cut(s) 6, 267, 704
SsiI CCGC 5 cut(s) 300, 340, 389, 695, 711
SspI AATATT 1 cut(s) 324
SspMI CTAG 2 cut(s) 492, 509
StyD4I CCNGG 4 cut(s) 39, 102, 261, 478
TaaI ACNGT 2 cut(s) 335, 734
TaiI ACGT 1 cut(s) 588
TasI AATT 3 cut(s) 6, 267, 704
TatI WGTACW 2 cut(s) 400, 596
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 2 cut(s) 365, 646
Tru1I TTAA 5 cut(s) 5, 198, 329, 660, 707
Tru9I TTAA 5 cut(s) 5, 198, 329, 660, 707
TscAI CASTG 2 cut(s) 625, 726
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 2 cut(s) 441, 505
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 738
TspRI CASTG 2 cut(s) 625, 726
Vha464I CTTAAG 1 cut(s) 197
XagI CCTNNNNNAGG 2 cut(s) 200, 250
XceI RCATGY 1 cut(s) 478
XmiI GTMKAC 3 cut(s) 305, 582, 588
XspI CTAG 2 cut(s) 492, 509
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.