pycom15g33130

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004594
Physical Location & Seq
Forward (+)
32475445 .. 32478656
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33130.4

Sequence Viewer

Length: 849 bp
ATGGATAGGTTATGCTGTTTTAGTTCTTCCCACTCTCAGTATCGGGGCCGTTCATCATGTGGCTCTGGTAAGGGAAGAAACAATGATGGGTTAATAAAGTATGGCTTCAGCCTGGTGAAAGGAAAAGCAAATCATCCAATGGAGGATTATCATGTTGCTAAGTTTGTTCGGACCCAGGGACATGAGTTAGGATTGTTTGCTATTTATGATGGTCATTTGGGTGATAATGTGCCTGCATACCTACAGAAGCATTTATTTTCAAATATCCTTAAGGAGGAAGAGTTTTGGATTGATCCTAACAGATCTATCTCAAAAGCCTATGAGAGGACAGACCAGGCAATTCTCTCTCACAGTTCTGACTTGGGGCGTGGAGGGTCAACTGCTGTAACTGCAATATTGTTAAACGGTCAGCGATTATGGGTGGCAAATGTTGGAGATTCACGGGCAGTTCTTTCAAGGGGGGGTCAGGCAGTTCAGGTGTCTACAGACCATGAGCCCAATACCGAAAGAGGCAGCATTGAAAACAAAGGTGGCTTTGTCTCTAACATGCCAGGAGATGTTCCTAGAGTCAATGGGCAGCTGGCAGTTTCTCGTGCTTTTGGAGACAAAAGCCTGAAGTCACATTTGCGATCGGATCCGGACATACAAGATATCTACGTAGACAATAGTACTGATGTTCTAGTCATTGCCAGTGATGGTCTTTGGAAGGTCATGGCAAATCAAGAGGCCGTTGATATGGCAAGAAAGACTAAAGACCCTCTGAAGGCGGCAAAGCAATTAACCGCAGAAGCGCTGAAAAGAGACAGTAAAGATGATATTTCCTGCGTCGTTGTTAGACTAAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

283

Amino Acids

30.9

Weight (kDa)

8.59

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 482, 660
AccIII TCCGGA 1 cut(s) 637
AciI CCGC 2 cut(s) 767, 783
AclWI GGATC 3 cut(s) 287, 629, 642
AcuI CTGAAG 3 cut(s) 91, 635, 782
AfaI GTAC 1 cut(s) 670
AfeI AGCGCT 1 cut(s) 792
AfiI CCNNNNNNNGG 3 cut(s) 274, 324, 763
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 3 cut(s) 261, 456, 521
AjnI CCWGG 4 cut(s) 111, 174, 333, 550
AluBI AGCT 1 cut(s) 580
AluI AGCT 1 cut(s) 580
Alw26I GTCTC 3 cut(s) 544, 597, 795
AlwI GGATC 3 cut(s) 287, 629, 642
Aor13HI TCCGGA 1 cut(s) 637
Aor51HI AGCGCT 1 cut(s) 792
AoxI GGCC 2 cut(s) 46, 726
ApeKI GCWGC 2 cut(s) 513, 577
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 2 cut(s) 46, 171
AsuHPI GGTGA 2 cut(s) 127, 233
AvaII GGWCC 1 cut(s) 171
BamHI GGATCC 1 cut(s) 634
BanII GRGCYC 1 cut(s) 498
BauI CACGAG 1 cut(s) 591
BbvI GCAGC 2 cut(s) 525, 589
BccI CCATC 3 cut(s) 80, 203, 689
BceAI ACGGC 2 cut(s) 33, 713
BciT130I CCWGG 4 cut(s) 113, 176, 335, 552
BcoDI GTCTC 3 cut(s) 544, 597, 795
BfaI CTAG 2 cut(s) 564, 680
BfmI CTRYAG 2 cut(s) 242, 483
BfoI RGCGCY 1 cut(s) 794
BfrI CTTAAG 1 cut(s) 269
BglII AGATCT 1 cut(s) 302
BisI GCNGC 3 cut(s) 514, 578, 768
BlsI GCNGC 3 cut(s) 515, 579, 769
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 4 cut(s) 113, 176, 335, 552
Bme18I GGWCC 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 46, 171
BmiI GGNNCC 3 cut(s) 47, 173, 636
BmrFI CCNGG 4 cut(s) 113, 176, 335, 552
BsaAI YACGTR 1 cut(s) 658
BsaJI CCNNGG 2 cut(s) 174, 175
BsaWI WCCGGW 1 cut(s) 637
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 324, 763
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 637
BseBI CCWGG 4 cut(s) 113, 176, 335, 552
BseDI CCNNGG 2 cut(s) 174, 175
BseGI GGATG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 274, 324, 763
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 690
BseXI GCAGC 2 cut(s) 525, 589
Bsh1285I CGRYCG 1 cut(s) 632
BshFI GGCC 2 cut(s) 48, 728
BsiEI CGRYCG 1 cut(s) 632
BsiSI CCGG 1 cut(s) 638
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 3 cut(s) 274, 324, 763
BsmAI GTCTC 3 cut(s) 544, 597, 795
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 2 cut(s) 48, 728
Bsp1286I GDGCHC 1 cut(s) 498
Bsp13I TCCGGA 1 cut(s) 637
Bsp143I GATC 4 cut(s) 292, 302, 629, 634
BspACI CCGC 2 cut(s) 767, 783
BspANI GGCC 2 cut(s) 48, 728
BspCNI CTCAG 1 cut(s) 49
BspEI TCCGGA 1 cut(s) 637
BspLI GGNNCC 3 cut(s) 47, 173, 636
BspPI GGATC 3 cut(s) 287, 629, 642
BspTI CTTAAG 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 2 cut(s) 174, 175
BssMI GATC 4 cut(s) 292, 302, 629, 634
BssSI CACGAG 1 cut(s) 591
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 4 cut(s) 113, 176, 335, 552
Bst4CI ACNGT 3 cut(s) 353, 407, 806
Bst6I CTCTTC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 234, 582
BstDEI CTNAG 3 cut(s) 36, 159, 839
BstENI CCTNNNNNAGG 2 cut(s) 272, 322
BstF5I GGATG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 4 cut(s) 295, 305, 632, 637
BstMAI GTCTC 3 cut(s) 544, 597, 795
BstMBI GATC 4 cut(s) 292, 302, 629, 634
BstMCI CGRYCG 1 cut(s) 632
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 4 cut(s) 113, 176, 335, 552
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 4 cut(s) 111, 174, 333, 550
BstSFI CTRYAG 2 cut(s) 242, 483
BstSNI TACGTA 1 cut(s) 658
BstV1I GCAGC 2 cut(s) 525, 589
BstX2I RGATCY 2 cut(s) 302, 634
BstYI RGATCY 2 cut(s) 302, 634
BsuRI GGCC 2 cut(s) 48, 728
BtsCI GGATG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 234, 582
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 46, 171
CseI GACGC 1 cut(s) 814
Csp6I GTAC 1 cut(s) 669
CviAII CATG 6 cut(s) 57, 152, 182, 491, 547, 712
CviQI GTAC 1 cut(s) 669
DdeI CTNAG 3 cut(s) 36, 159, 839
DpnI GATC 4 cut(s) 294, 304, 631, 636
DpnII GATC 4 cut(s) 292, 302, 629, 634
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco105I TACGTA 1 cut(s) 658
Eco24I GRGCYC 1 cut(s) 498
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 171
Eco47III AGCGCT 1 cut(s) 792
Eco57I CTGAAG 3 cut(s) 91, 635, 782
EcoNI CCTNNNNNAGG 2 cut(s) 272, 322
EcoRII CCWGG 4 cut(s) 111, 174, 333, 550
EcoRV GATATC 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 498
FaeI CATG 6 cut(s) 60, 155, 185, 494, 550, 715
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 192
FatI CATG 6 cut(s) 56, 151, 181, 490, 546, 711
FblI GTMKAC 2 cut(s) 482, 660
Fnu4HI GCNGC 3 cut(s) 514, 578, 768
FokI GGATG 1 cut(s) 120
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 3 cut(s) 514, 578, 768
FspBI CTAG 2 cut(s) 564, 680
GlaI GCGC 1 cut(s) 792
GluI GCNGC 3 cut(s) 514, 578, 768
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 2 cut(s) 48, 728
HapII CCGG 1 cut(s) 638
HgaI GACGC 1 cut(s) 814
HhaI GCGC 1 cut(s) 793
Hin1II CATG 6 cut(s) 60, 155, 185, 494, 550, 715
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 2 cut(s) 437, 567
HpaII CCGG 1 cut(s) 638
HphI GGTGA 2 cut(s) 127, 233
Hpy166II GTNNAC 3 cut(s) 378, 483, 661
Hpy188I TCNGA 4 cut(s) 171, 358, 634, 762
Hpy188III TCNNGA 2 cut(s) 638, 722
Hpy8I GTNNAC 3 cut(s) 378, 483, 661
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 2 cut(s) 700, 757
HpyCH4III ACNGT 3 cut(s) 353, 407, 806
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 2 cut(s) 236, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 3 cut(s) 36, 159, 839
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 6 cut(s) 60, 155, 185, 494, 550, 715
HspAI GCGC 1 cut(s) 791
Kpn2I TCCGGA 1 cut(s) 637
Kzo9I GATC 4 cut(s) 292, 302, 629, 634
Lsp1109I GCAGC 2 cut(s) 525, 589
MaeI CTAG 2 cut(s) 564, 680
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 2 cut(s) 385, 618
MalI GATC 4 cut(s) 294, 304, 631, 636
MboI GATC 4 cut(s) 292, 302, 629, 634
MboII GAAGA 3 cut(s) 18, 87, 290
MflI RGATCY 2 cut(s) 302, 634
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 2 cut(s) 339, 776
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 7 cut(s) 136, 268, 318, 365, 503, 718, 768
MroI TCCGGA 1 cut(s) 637
MseI TTAA 4 cut(s) 92, 270, 401, 779
MslI CAYNNNNRTG 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 580
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 1 cut(s) 638
MspR9I CCNGG 4 cut(s) 113, 176, 335, 552
MvaI CCWGG 4 cut(s) 113, 176, 335, 552
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 4 cut(s) 292, 302, 629, 634
NlaIII CATG 6 cut(s) 60, 155, 185, 494, 550, 715
NlaIV GGNNCC 3 cut(s) 47, 173, 636
NmuCI GTSAC 1 cut(s) 618
NspI RCATGY 1 cut(s) 550
PasI CCCWGGG 1 cut(s) 175
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 3 cut(s) 515, 579, 769
Ple19I CGATCG 1 cut(s) 632
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
Ppu21I YACGTR 1 cut(s) 658
Psp6I CCWGG 4 cut(s) 111, 174, 333, 550
PspGI CCWGG 4 cut(s) 111, 174, 333, 550
PspN4I GGNNCC 3 cut(s) 47, 173, 636
PspPI GGNCC 2 cut(s) 46, 171
PsuI RGATCY 2 cut(s) 302, 634
PvuI CGATCG 1 cut(s) 632
PvuII CAGCTG 1 cut(s) 580
RsaI GTAC 1 cut(s) 670
RsaNI GTAC 1 cut(s) 669
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 4 cut(s) 92, 270, 401, 779
SatI GCNGC 3 cut(s) 514, 578, 768
Sau3AI GATC 4 cut(s) 292, 302, 629, 634
Sau96I GGNCC 2 cut(s) 46, 171
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 4 cut(s) 113, 176, 335, 552
SduI GDGCHC 1 cut(s) 498
SetI ASST 7 cut(s) 11, 243, 480, 532, 582, 660, 711
SfcI CTRYAG 2 cut(s) 242, 483
SinI GGWCC 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SnaBI TACGTA 1 cut(s) 658
Sse9I AATT 2 cut(s) 339, 776
SsiI CCGC 2 cut(s) 767, 783
SspI AATATT 1 cut(s) 396
SspMI CTAG 2 cut(s) 564, 680
StyD4I CCNGG 4 cut(s) 111, 174, 333, 550
TaaI ACNGT 3 cut(s) 353, 407, 806
TaiI ACGT 1 cut(s) 660
TasI AATT 2 cut(s) 339, 776
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 4 cut(s) 92, 270, 401, 779
Tru9I TTAA 4 cut(s) 92, 270, 401, 779
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 1 cut(s) 618
TseI GCWGC 2 cut(s) 513, 577
Tsp45I GTSAC 1 cut(s) 618
TspDTI ATGAA 1 cut(s) 42
TspRI CASTG 1 cut(s) 697
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 171
XagI CCTNNNNNAGG 2 cut(s) 272, 322
XceI RCATGY 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 482, 660
XspI CTAG 2 cut(s) 564, 680
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.