FvH4_5g37270

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004594
Physical Location & Seq
Reverse (-)
27403824 .. 27409233
5410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37270.t4

Sequence Viewer

Length: 849 bp
ATGGACAGGTTGTGCTGTTTTAGTGCTTCCAATTCTCAGTATGGAGGACGGTCATCGTCAAGCTCTGGCAAGGGAAAAAGTAACGAGGGGTTAATCAAGTTTGGTTTCAGCCTGGTAAAAGGAAAAGCTAATCATCCAATGGAGGATTATCATGTTGCTAAGTTCGTTAGGTCACAGGGACATGAGTTAGGATTGTTTGCTATATATGATGGCCATTTGGGTGATACAGTGCCTCAGTACTTACAGAAGCATTTGTTTCCAAATATTCTTAAGGAGGAAGAATTTTGGGTTGACCCTAACAGATCCATCTCAAAAGCCTATGAGAGGACAGACCAGGCAATCCTCTCACATAGCACTGACTTGGGGCGTGGGGGGTCCACTGCTGTCACTGCAATATTGTTAAATGGTCAGCGGTTATGGGTGGCCAATGTCGGAGATTCACGGGCAGTTCTTTCAAAGGAGGGTCAGGCAGTACAAATGTCAACAGACCATGAACCCAACACTGAACGAGGCAGCATTGAAAACAAAGGGGGCTTTGTCTCAAACATGCCAGGAGATGTACCTAGAGTTAATGGGCAGCTGGCAGTTTCTCGTGCTTTTGGAGACAAGAGCCTCAAGTCGCATTTACGATCAGACCCAGACATACAGGATACTTACATAGACACAAATACAGATGTTCTAATCCTTGCAAGTGACGGCCTTTGGAAGGTCATGGGTAATCAAGAGGCAGTTGATATTGCAAGAAAAAATAAAGACCCTATGAAGGCGGCTAAGGAGTTAACAGCTGAAGCTTTGAAAAGAGATAGTAAAGATGATATATCTTGCGTTGTTGTTAGATTTAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

283

Amino Acids

30.9

Weight (kDa)

7.09

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 42 - 273 2.2e-57 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 412, 767
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 211, 423
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 807
AfaI GTAC 3 cut(s) 239, 474, 561
AfiI CCNNNNNNNGG 3 cut(s) 324, 706, 763
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 3 cut(s) 456, 521, 796
AjnI CCWGG 3 cut(s) 111, 333, 550
AluBI AGCT 5 cut(s) 63, 128, 580, 785, 791
AluI AGCT 5 cut(s) 63, 128, 580, 785, 791
Alw26I GTCTC 2 cut(s) 544, 597
AlwI GGATC 1 cut(s) 297
AoxI GGCC 3 cut(s) 211, 423, 697
ApeKI GCWGC 2 cut(s) 513, 577
ApoI RAATTY 1 cut(s) 281
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 375
BalI TGGCCA 2 cut(s) 213, 425
BauI CACGAG 1 cut(s) 591
BbvI GCAGC 2 cut(s) 525, 589
BccI CCATC 2 cut(s) 203, 314
BceAI ACGGC 1 cut(s) 712
BciT130I CCWGG 3 cut(s) 113, 335, 552
BciVI GTATCC 1 cut(s) 643
BcoDI GTCTC 2 cut(s) 544, 597
BfaI CTAG 1 cut(s) 564
BfrI CTTAAG 1 cut(s) 269
BfuI GTATCC 1 cut(s) 643
BisI GCNGC 3 cut(s) 514, 578, 768
BlsI GCNGC 3 cut(s) 515, 579, 769
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 3 cut(s) 113, 335, 552
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 3 cut(s) 113, 335, 552
Bpu10I CCTNAGC 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 599
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 706, 763
BseBI CCWGG 3 cut(s) 113, 335, 552
BseGI GGATG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 324, 706, 763
BseMII CTCAG 2 cut(s) 50, 248
BseXI GCAGC 2 cut(s) 525, 589
BshFI GGCC 3 cut(s) 213, 425, 699
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 3 cut(s) 324, 706, 763
BsmAI GTCTC 2 cut(s) 544, 597
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 3 cut(s) 213, 425, 699
Bsp143I GATC 2 cut(s) 302, 629
BspACI CCGC 2 cut(s) 412, 767
BspANI GGCC 3 cut(s) 213, 425, 699
BspCNI CTCAG 2 cut(s) 49, 247
BspLI GGNNCC 1 cut(s) 376
BspPI GGATC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 269
BssMI GATC 2 cut(s) 302, 629
BssSI CACGAG 1 cut(s) 591
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 3 cut(s) 113, 335, 552
Bst4CI ACNGT 2 cut(s) 51, 229
BstAFI CTTAAG 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 4 cut(s) 36, 159, 234, 771
BstENI CCTNNNNNAGG 2 cut(s) 322, 704
BstF5I GGATG 1 cut(s) 133
BstKTI GATC 2 cut(s) 305, 632
BstMAI GTCTC 2 cut(s) 544, 597
BstMBI GATC 2 cut(s) 302, 629
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 3 cut(s) 113, 335, 552
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 3 cut(s) 111, 333, 550
BstV1I GCAGC 2 cut(s) 525, 589
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 3 cut(s) 213, 425, 699
BtsCI GGATG 1 cut(s) 133
BtsI GCAGTG 2 cut(s) 378, 387
BtsIMutI CAGTG 5 cut(s) 234, 354, 378, 387, 501
Cac8I GCNNGC 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 3 cut(s) 238, 473, 560
CviAII CATG 5 cut(s) 152, 182, 491, 547, 712
CviQI GTAC 3 cut(s) 238, 473, 560
DdeI CTNAG 4 cut(s) 36, 159, 234, 771
DpnI GATC 2 cut(s) 304, 631
DpnII GATC 2 cut(s) 302, 629
EaeI YGGCCR 2 cut(s) 211, 423
Eco47I GGWCC 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 807
EcoNI CCTNNNNNAGG 2 cut(s) 322, 704
EcoRII CCWGG 3 cut(s) 111, 333, 550
FaeI CATG 5 cut(s) 155, 185, 494, 550, 715
FaqI GGGAC 1 cut(s) 192
FatI CATG 5 cut(s) 151, 181, 490, 546, 711
Fnu4HI GCNGC 3 cut(s) 514, 578, 768
FokI GGATG 1 cut(s) 120
Fsp4HI GCNGC 3 cut(s) 514, 578, 768
FspBI CTAG 1 cut(s) 564
GluI GCNGC 3 cut(s) 514, 578, 768
HaeIII GGCC 3 cut(s) 213, 425, 699
Hin1II CATG 5 cut(s) 155, 185, 494, 550, 715
HincII GTYRAC 3 cut(s) 292, 483, 780
HindII GTYRAC 3 cut(s) 292, 483, 780
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 1 cut(s) 437
HpaI GTTAAC 1 cut(s) 780
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 4 cut(s) 292, 378, 483, 780
Hpy188I TCNGA 2 cut(s) 434, 634
Hpy188III TCNNGA 1 cut(s) 722
Hpy8I GTNNAC 4 cut(s) 292, 378, 483, 780
HpyAV CCTTC 2 cut(s) 700, 757
HpyCH4III ACNGT 2 cut(s) 51, 229
HpyCH4V TGCA 3 cut(s) 392, 689, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 4 cut(s) 36, 159, 234, 771
Hsp92II CATG 5 cut(s) 155, 185, 494, 550, 715
KspAI GTTAAC 1 cut(s) 780
Kzo9I GATC 2 cut(s) 302, 629
Lsp1109I GCAGC 2 cut(s) 525, 589
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 4 cut(s) 80, 171, 385, 692
MalI GATC 2 cut(s) 304, 631
MboI GATC 2 cut(s) 302, 629
MboII GAAGA 1 cut(s) 290
MflI RGATCY 1 cut(s) 302
MlsI TGGCCA 2 cut(s) 213, 425
MluCI AATT 2 cut(s) 31, 281
MluNI TGGCCA 2 cut(s) 213, 425
MmeI TCCRAC 1 cut(s) 412
Mox20I TGGCCA 2 cut(s) 213, 425
MscI TGGCCA 2 cut(s) 213, 425
MseI TTAA 5 cut(s) 92, 270, 401, 570, 779
MslI CAYNNNNRTG 1 cut(s) 219
Msp20I TGGCCA 2 cut(s) 213, 425
MspA1I CMGCKG 3 cut(s) 412, 580, 785
MspCI CTTAAG 1 cut(s) 269
MspR9I CCNGG 3 cut(s) 113, 335, 552
MvaI CCWGG 3 cut(s) 113, 335, 552
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeII GATC 2 cut(s) 302, 629
NlaIII CATG 5 cut(s) 155, 185, 494, 550, 715
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 3 cut(s) 171, 385, 692
NspI RCATGY 1 cut(s) 550
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 3 cut(s) 515, 579, 769
Psp6I CCWGG 3 cut(s) 111, 333, 550
PspGI CCWGG 3 cut(s) 111, 333, 550
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 375
PsuI RGATCY 1 cut(s) 302
PvuII CAGCTG 2 cut(s) 580, 785
RsaI GTAC 3 cut(s) 239, 474, 561
RsaNI GTAC 3 cut(s) 238, 473, 560
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 5 cut(s) 92, 270, 401, 570, 779
SatI GCNGC 3 cut(s) 514, 578, 768
Sau3AI GATC 2 cut(s) 302, 629
Sau96I GGNCC 1 cut(s) 375
ScaI AGTACT 1 cut(s) 239
ScrFI CCNGG 3 cut(s) 113, 335, 552
SetI ASST 9 cut(s) 11, 65, 130, 173, 565, 582, 711, 787, 793
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 269, 614
SmoI CTYRAG 2 cut(s) 269, 614
Sse9I AATT 2 cut(s) 31, 281
SsiI CCGC 2 cut(s) 412, 767
SspI AATATT 2 cut(s) 265, 396
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 3 cut(s) 111, 333, 550
TaaI ACNGT 2 cut(s) 51, 229
TasI AATT 2 cut(s) 31, 281
TatI WGTACW 2 cut(s) 237, 472
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 5 cut(s) 92, 270, 401, 570, 779
Tru9I TTAA 5 cut(s) 92, 270, 401, 570, 779
TscAI CASTG 5 cut(s) 234, 361, 385, 394, 508
TseFI GTSAC 3 cut(s) 171, 385, 692
TseI GCWGC 2 cut(s) 513, 577
Tsp45I GTSAC 3 cut(s) 171, 385, 692
TspDTI ATGAA 2 cut(s) 507, 776
TspRI CASTG 5 cut(s) 234, 361, 385, 394, 508
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 375
XagI CCTNNNNNAGG 2 cut(s) 322, 704
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 550
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.