Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011632

Physical Location & Seq

Genomic Coordinates Forward (+)

13205345 .. 13208289

Gene Structure 2945 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G35670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGGAGACGAAGCCAAACCCTAGACGTCCTTCAAACACAGTTCTACCATATCAAACACCACGATTAAGAGATCATTACCTTCTGGGAAAAAAGCTAGGCCAAGGCCAATTTGGAACAACCTATCTCTGCACAGAGAAATCAACCTCCGCTAATTACGCCTGCAAATCGATCCCGAAGCGAAAGCTCGTGTGTCGCGAGGATTACGAAGATGTATGGCGTGAGATTCAGATCATGCATCATCTCTCTGAGCATCCAAATGTTGTTAGGATCAAAGGGACTTATGAAGATTCGGTGTTTGTTCATATTGTTATGGAGGTTTGTGAAGGTGGTGAGCTTTTTGATCGGATTGTTTCTAAAGGTCATTTTAGTGAGCGTGAAGCTGTCAAGCTTATTAAGACGATTCTTGGTGTTGTTGAGGCTTGTCATTCTCTTGGTGTTATGCATAGAGATCTCAAACCTGAGAATTTCTTGTTTGATAGTCCTAAAGATGATGCTAAGCTTAAGGCTACCGATTTTGGTTTGTCTGTCTTCTATAAGCCAGGACAATATTTATATGACGTAGTTGGAAGTCCGTACTATGTTGCACCAGAGGTGCTAAAGAAATGTTATGGACCTGAAATAGATGTGTGGAGTGCTGGTGTTATCCTCTACATTTTACTCAGCGGTGTTCCTCCCTTCTGGGCAGAGACTGAGTCTGGAATCTTTAGACAGATATTGCAAGGGAAGTTAGATTTCAAATCTGACCCGTGGCCTACTATCTCAGAAGCTGCTAAAGATTTGATCTATAAAATGCTCGAAAGGAGCCCCAAGAAACGCATTTCTGCTCATGAAGCCTTGTGTCACCCATGGATTGTCGATGAACAAGCAGCACCAGACAAGCCTCTTGATCCAGCAGTCTTATCTCGTCTAAAGCAGTTTTCTCAAATGAATAAGATTAAGAAAATGGCATTACGGGTAATTGCTGAGAGACTTTCAGAGGAAGAAATTGGAGGTCTGAAGGAATTGTTCAAGATGATAGACACAGACAACAGCGGAACGATTACTTTTGAAGAGCTCAAAGCGGGTTTGAAGAGAGTCGGATCTGAACTGATGGAATCAGAAATCAAGTCTCTCATGGATGCGGCTGATATCGACAACAGTGGTACAATAGACTACGGAGAATTCCTAGCAGCAACCTTACACATGAACAAGATGGAGAGAGAGGAGAATCTGGTGGCTGCATTTTCGTACTTTGACAAAGACGGAAGCGGTTATATCACCATCGATGAGCTTCAGTCAGCTTGCACAGAGTTTGGTCTATGTGATACACCTCTGGACGACATGATCAAGGAGATTGATCTTGACAATGACGGGAAGATCGATTTCTCGGAGTTTACAGCAATGATGAGGAAAGGAGATGGAGTTGGGAGAAGCAGAACCATGATGAAGAACTTGAACTTCAACATTGCTGATGCTTTTGGAGTTGATGGTGAAAAATCTGATGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009787 GO:0009789 GO:0009931 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023051 GO:0023052 GO:0023056 GO:0032409 GO:0032412 GO:0032879 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080092 GO:0140096 GO:1901016 GO:1901379 GO:1901419 GO:1901421 GO:1901564 GO:1901979 GO:1904062 GO:1905957 GO:1905959 GO:2000241 GO:2001257

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

495

Amino Acids

55.92

Weight (kDa)

5.27

Isoelectric Point (pI)

43.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	28 - 284	7.2e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	29 - 276	1.4e-26	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	332 - 358	4.4e-08	EF hand domain
EF-hand_7	PF13499	332 - 392	2.3e-12	EF-hand domain pair
EF-hand_6	PF13405	332 - 360	1.1e-07	EF-hand domain
EF-hand_5	PF13202	332 - 354	3.4e-06	EF hand
EF-hand_8	PF13833	345 - 391	2.5e-06	EF-hand domain pair
EF-hand_7	PF13499	402 - 463	1.4e-15	EF-hand domain pair
EF-hand_6	PF13405	404 - 432	6.5e-07	EF-hand domain
EF-hand_1	PF00036	406 - 430	1.7e-06	EF hand domain
EF-hand_1	PF00036	438 - 464	5.3e-08	EF hand domain
EF-hand_8	PF13833	438 - 464	2.7e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35670 AT4G09570
fragaria_vesca	FvH4_2g11790
malus_domestica	MD05G1041800.v1.1 MD10G1048300.v1.1
prunus_persica	Prupe.8G057600_v2.0.a1
pyrus_communis	pycom10g03420
rosa_chinensis	RchiOBHm_Chr6g0272011
rosa_laevigata	RLG00000013709
rosa_multiflora	Rmu_co8430291.1_g000001 Rmu_sc0014039.1_g000006
rosa_roxburghii	Rroxscaffold_173G00434970 Rroxscaffold_7G00196830
rosa_rugosa	Rorug06G0064900
rosa_samantha	Rh6BG184900 Rh6CG183500 Rh6DG175000
rosa_wichuraiana	Rw0G003460 Rw6G015690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	28
AccII	CGCG	1 cut(s)	195
AciI	CCGC	6 cut(s)	147, 663, 1032, 1061, 1121, 1248
AclWI	GGATC	4 cut(s)	163, 275, 881, 1087
AcsI	RAATTY	2 cut(s)	463, 1160
AcuI	CTGAAG	2 cut(s)	1016, 1256
AcyI	GRCGYC	1 cut(s)	25
AfaI	GTAC	3 cut(s)	575, 1144, 1229
AflII	CTTAAG	1 cut(s)	500
AgsI	TTSAA	7 cut(s)	33, 736, 1009, 1049, 1069, 1435, 1441
AjnI	CCWGG	1 cut(s)	538
Alw21I	GWGCWC	1 cut(s)	1056
Alw26I	GTCTC	3 cut(s)	680, 961, 1113
AlwI	GGATC	4 cut(s)	163, 275, 881, 1087
AlwNI	CAGNNNCTG	2 cut(s)	689, 767
AoxI	GGCC	3 cut(s)	97, 103, 749
ApeKI	GCWGC	4 cut(s)	767, 866, 1169, 1217
ApoI	RAATTY	2 cut(s)	463, 1160
AspS9I	GGNCC	1 cut(s)	611
AsuHPI	GGTGA	4 cut(s)	341, 833, 1249, 1481
AvaII	GGWCC	1 cut(s)	611
BanII	GRGCYC	2 cut(s)	806, 1056
BauI	CACGAG	1 cut(s)	185
BbsI	GAAGAC	1 cut(s)	520
Bbv12I	GWGCWC	1 cut(s)	1056
BbvI	GCAGC	4 cut(s)	754, 878, 1181, 1204
BccI	CCATC	5 cut(s)	1084, 1186, 1268, 1391, 1460
BciT130I	CCWGG	1 cut(s)	540
BclI	TGATCA	1 cut(s)	1323
BcoDI	GTCTC	3 cut(s)	680, 961, 1113
BfaI	CTAG	3 cut(s)	21, 95, 1166
BfrI	CTTAAG	1 cut(s)	500
BglII	AGATCT	1 cut(s)	448
BisI	GCNGC	5 cut(s)	768, 867, 1122, 1170, 1218
BlpI	GCTNAGC	1 cut(s)	495
BlsI	GCNGC	5 cut(s)	769, 868, 1123, 1171, 1219
Bme1390I	CCNGG	1 cut(s)	540
Bme18I	GGWCC	1 cut(s)	611
BmgT120I	GGNCC	1 cut(s)	611
BmiI	GGNNCC	1 cut(s)	803
BmrFI	CCNGG	1 cut(s)	540
BmsI	GCATC	5 cut(s)	244, 259, 481, 1108, 1441
BpiI	GAAGAC	1 cut(s)	520
Bpu1102I	GCTNAGC	1 cut(s)	495
Bsa29I	ATCGAT	3 cut(s)	167, 1263, 1359
BsaBI	GATNNNNATC	1 cut(s)	227
BsaHI	GRCGYC	1 cut(s)	25
BsaJI	CCNNGG	3 cut(s)	100, 746, 845
BsaXI	ACNNNNNCTCC	4 cut(s)	622, 652, 1386, 1416
Bse3DI	GCAATG	2 cut(s)	1386, 1443
Bse8I	GATNNNNATC	1 cut(s)	227
BseBI	CCWGG	1 cut(s)	540
BseCI	ATCGAT	3 cut(s)	167, 1263, 1359
BseDI	CCNNGG	3 cut(s)	100, 746, 845
BseGI	GGATG	2 cut(s)	250, 1123
BseJI	GATNNNNATC	1 cut(s)	227
BseMI	GCAATG	2 cut(s)	1386, 1443
BseMII	CTCAG	6 cut(s)	237, 450, 673, 681, 774, 954
BseRI	GAGGAG	1 cut(s)	1217
BseXI	GCAGC	4 cut(s)	754, 878, 1181, 1204
BsgI	GTGCAG	1 cut(s)	112
Bsh1236I	CGCG	1 cut(s)	195
BshFI	GGCC	3 cut(s)	99, 105, 751
BshVI	ATCGAT	3 cut(s)	167, 1263, 1359
BsiHKAI	GWGCWC	1 cut(s)	1056
BslFI	GGGAC	1 cut(s)	289
BsmAI	GTCTC	3 cut(s)	680, 961, 1113
BsmFI	GGGAC	1 cut(s)	289
BsnI	GGCC	3 cut(s)	99, 105, 751
Bsp1286I	GDGCHC	2 cut(s)	806, 1056
Bsp1720I	GCTNAGC	1 cut(s)	495
Bsp19I	CCATGG	1 cut(s)	845
Bsp68I	TCGCGA	1 cut(s)	195
BspACI	CCGC	6 cut(s)	147, 663, 1032, 1061, 1121, 1248
BspANI	GGCC	3 cut(s)	99, 105, 751
BspCNI	CTCAG	6 cut(s)	238, 451, 672, 682, 773, 955
BspDI	ATCGAT	3 cut(s)	167, 1263, 1359
BspFNI	CGCG	1 cut(s)	195
BspHI	TCATGA	1 cut(s)	826
BspLI	GGNNCC	1 cut(s)	803
BspPI	GGATC	4 cut(s)	163, 275, 881, 1087
BspQI	GCTCTTC	1 cut(s)	1044
BspTI	CTTAAG	1 cut(s)	500
BsrDI	GCAATG	2 cut(s)	1386, 1443
BssECI	CCNNGG	3 cut(s)	100, 746, 845
BssNI	GRCGYC	1 cut(s)	25
BssSI	CACGAG	1 cut(s)	185
BssT1I	CCWWGG	2 cut(s)	100, 845
Bst2BI	CACGAG	1 cut(s)	185
Bst2UI	CCWGG	1 cut(s)	540
Bst4CI	ACNGT	2 cut(s)	40, 1139
Bst6I	CTCTTC	2 cut(s)	1044, 1064
BstACI	GRCGYC	1 cut(s)	25
BstAFI	CTTAAG	1 cut(s)	500
BstC8I	GCNNGC	2 cut(s)	160, 1282
BstDEI	CTNAG	7 cut(s)	246, 459, 495, 659, 690, 760, 963
BstDSI	CCRYGG	2 cut(s)	746, 845
BstF5I	GGATG	2 cut(s)	250, 1123
BstFNI	CGCG	1 cut(s)	195
BstMAI	GTCTC	3 cut(s)	680, 961, 1113
BstMWI	GCNNNNNNNGC	2 cut(s)	155, 830
BstNI	CCWGG	1 cut(s)	540
BstSCI	CCNGG	1 cut(s)	538
BstUI	CGCG	1 cut(s)	195
BstV1I	GCAGC	4 cut(s)	754, 878, 1181, 1204
BstV2I	GAAGAC	1 cut(s)	520
BstX2I	RGATCY	2 cut(s)	448, 1079
BstYI	RGATCY	2 cut(s)	448, 1079
Bsu15I	ATCGAT	3 cut(s)	167, 1263, 1359
BsuRI	GGCC	3 cut(s)	99, 105, 751
BsuTUI	ATCGAT	3 cut(s)	167, 1263, 1359
BtgI	CCRYGG	2 cut(s)	746, 845
BtsCI	GGATG	2 cut(s)	250, 1123
BtsIMutI	CAGTG	1 cut(s)	1144
BtuMI	TCGCGA	1 cut(s)	195
Cac8I	GCNNGC	2 cut(s)	160, 1282
CaiI	CAGNNNCTG	2 cut(s)	689, 767
CciI	TCATGA	1 cut(s)	826
Cfr13I	GGNCC	1 cut(s)	611
ClaI	ATCGAT	3 cut(s)	167, 1263, 1359
Csp6I	GTAC	3 cut(s)	574, 1143, 1228
CviAII	CATG	7 cut(s)	232, 827, 846, 1114, 1183, 1321, 1420
CviQI	GTAC	3 cut(s)	574, 1143, 1228
DdeI	CTNAG	7 cut(s)	246, 459, 495, 659, 690, 760, 963
Eam1104I	CTCTTC	2 cut(s)	1044, 1064
EarI	CTCTTC	2 cut(s)	1044, 1064
Ecl136II	GAGCTC	1 cut(s)	1054
Eco130I	CCWWGG	2 cut(s)	100, 845
Eco24I	GRGCYC	2 cut(s)	806, 1056
Eco32I	GATATC	1 cut(s)	1129
Eco47I	GGWCC	1 cut(s)	611
Eco53kI	GAGCTC	1 cut(s)	1054
Eco57I	CTGAAG	2 cut(s)	1016, 1256
EcoICRI	GAGCTC	1 cut(s)	1054
EcoRI	GAATTC	1 cut(s)	1160
EcoRII	CCWGG	1 cut(s)	538
EcoRV	GATATC	1 cut(s)	1129
EcoT14I	CCWWGG	2 cut(s)	100, 845
EcoT22I	ATGCAT	2 cut(s)	237, 444
EcoT38I	GRGCYC	2 cut(s)	806, 1056
ErhI	CCWWGG	2 cut(s)	100, 845
FaeI	CATG	7 cut(s)	235, 830, 849, 1117, 1186, 1324, 1423
FaqI	GGGAC	1 cut(s)	289
FatI	CATG	7 cut(s)	231, 826, 845, 1113, 1182, 1320, 1419
FauI	CCCGC	1 cut(s)	1054
FbaI	TGATCA	1 cut(s)	1323
Fnu4HI	GCNGC	5 cut(s)	768, 867, 1122, 1170, 1218
FokI	GGATG	2 cut(s)	237, 1130
FriOI	GRGCYC	2 cut(s)	806, 1056
Fsp4HI	GCNGC	5 cut(s)	768, 867, 1122, 1170, 1218
FspBI	CTAG	3 cut(s)	21, 95, 1166
GluI	GCNGC	5 cut(s)	768, 867, 1122, 1170, 1218
HaeIII	GGCC	3 cut(s)	99, 105, 751
Hin1I	GRCGYC	1 cut(s)	25
Hin1II	CATG	7 cut(s)	235, 830, 849, 1117, 1186, 1324, 1423
HindIII	AAGCTT	2 cut(s)	386, 497
HinfI	GANTC	8 cut(s)	223, 287, 400, 692, 699, 1074, 1094, 1207
HphI	GGTGA	4 cut(s)	341, 833, 1249, 1481
Hpy166II	GTNNAC	1 cut(s)	1374
Hpy188III	TCNNGA	8 cut(s)	172, 194, 696, 827, 884, 1009, 1313, 1340
Hpy8I	GTNNAC	1 cut(s)	1374
HpyAV	CCTTC	5 cut(s)	39, 89, 317, 685, 991
HpyCH4III	ACNGT	2 cut(s)	40, 1139
HpyCH4IV	ACGT	2 cut(s)	25, 558
HpyCH4V	TGCA	8 cut(s)	129, 162, 235, 442, 584, 718, 1220, 1284
HpyF10VI	GCNNNNNNNGC	2 cut(s)	155, 830
HpyF3I	CTNAG	7 cut(s)	246, 459, 495, 659, 690, 760, 963
HpySE526I	ACGT	2 cut(s)	25, 558
Hsp92I	GRCGYC	1 cut(s)	25
Hsp92II	CATG	7 cut(s)	235, 830, 849, 1117, 1186, 1324, 1423
Ksp22I	TGATCA	1 cut(s)	1323
LguI	GCTCTTC	1 cut(s)	1044
LmnI	GCTCC	1 cut(s)	801
Lsp1109I	GCAGC	4 cut(s)	754, 878, 1181, 1204
LweI	GCATC	5 cut(s)	244, 259, 481, 1108, 1441
MaeI	CTAG	3 cut(s)	21, 95, 1166
MaeII	ACGT	2 cut(s)	25, 558
MaeIII	GTNAC	1 cut(s)	839
MboII	GAAGA	8 cut(s)	218, 296, 520, 992, 1061, 1081, 1366, 1438
MflI	RGATCY	2 cut(s)	448, 1079
MhlI	GDGCHC	2 cut(s)	806, 1056
MluCI	AATT	7 cut(s)	107, 151, 463, 957, 984, 1001, 1160
MlyI	GAGTC	2 cut(s)	701, 1083
MmeI	TCCRAC	2 cut(s)	544, 1057
Mph1103I	ATGCAT	2 cut(s)	237, 444
MseI	TTAA	4 cut(s)	65, 393, 501, 936
MslI	CAYNNNNRTG	2 cut(s)	255, 366
MspA1I	CMGCKG	2 cut(s)	663, 1032
MspCI	CTTAAG	1 cut(s)	500
MspR9I	CCNGG	1 cut(s)	540
MvaI	CCWGG	1 cut(s)	540
MvnI	CGCG	1 cut(s)	195
MwoI	GCNNNNNNNGC	2 cut(s)	155, 830
NcoI	CCATGG	1 cut(s)	845
NlaIII	CATG	7 cut(s)	235, 830, 849, 1117, 1186, 1324, 1423
NlaIV	GGNNCC	1 cut(s)	803
NmuCI	GTSAC	1 cut(s)	839
NruI	TCGCGA	1 cut(s)	195
NsiI	ATGCAT	2 cut(s)	237, 444
PagI	TCATGA	1 cut(s)	826
PciSI	GCTCTTC	1 cut(s)	1044
PcsI	WCGNNNNNNNCGW	2 cut(s)	192, 1356
PfeI	GAWTC	6 cut(s)	223, 287, 400, 699, 1094, 1207
PflFI	GACNNNGTC	1 cut(s)	691
PkrI	GCNGC	5 cut(s)	769, 868, 1123, 1171, 1219
PleI	GAGTC	2 cut(s)	700, 1082
PpsI	GAGTC	2 cut(s)	700, 1082
Psp124BI	GAGCTC	1 cut(s)	1056
Psp6I	CCWGG	1 cut(s)	538
PspGI	CCWGG	1 cut(s)	538
PspN4I	GGNNCC	1 cut(s)	803
PspPI	GGNCC	1 cut(s)	611
PstNI	CAGNNNCTG	2 cut(s)	689, 767
PsuI	RGATCY	2 cut(s)	448, 1079
PsyI	GACNNNGTC	1 cut(s)	691
RruI	TCGCGA	1 cut(s)	195
RsaI	GTAC	3 cut(s)	575, 1144, 1229
RsaNI	GTAC	3 cut(s)	574, 1143, 1228
RseI	CAYNNNNRTG	2 cut(s)	255, 366
SacI	GAGCTC	1 cut(s)	1056
SapI	GCTCTTC	1 cut(s)	1044
SaqAI	TTAA	4 cut(s)	65, 393, 501, 936
SatI	GCNGC	5 cut(s)	768, 867, 1122, 1170, 1218
Sau96I	GGNCC	1 cut(s)	611
SchI	GAGTC	2 cut(s)	701, 1083
ScrFI	CCNGG	1 cut(s)	540
SduI	GDGCHC	2 cut(s)	806, 1056
SfaNI	GCATC	5 cut(s)	244, 259, 481, 1108, 1441
SinI	GGWCC	1 cut(s)	611
SmiMI	CAYNNNNRTG	2 cut(s)	255, 366
SmlI	CTYRAG	1 cut(s)	500
SmoI	CTYRAG	1 cut(s)	500
Sse9I	AATT	7 cut(s)	107, 151, 463, 957, 984, 1001, 1160
SsiI	CCGC	6 cut(s)	147, 663, 1032, 1061, 1121, 1248
SspI	AATATT	1 cut(s)	548
SspMI	CTAG	3 cut(s)	21, 95, 1166
SstI	GAGCTC	1 cut(s)	1056
StyD4I	CCNGG	1 cut(s)	538
StyI	CCWWGG	2 cut(s)	100, 845
TaaI	ACNGT	2 cut(s)	40, 1139
TaiI	ACGT	2 cut(s)	28, 561
TaqI	TCGA	6 cut(s)	167, 795, 855, 1131, 1263, 1359
TasI	AATT	7 cut(s)	107, 151, 463, 957, 984, 1001, 1160
TauI	GCSGC	1 cut(s)	1124
TfiI	GAWTC	6 cut(s)	223, 287, 400, 699, 1094, 1207
Tru1I	TTAA	4 cut(s)	65, 393, 501, 936
Tru9I	TTAA	4 cut(s)	65, 393, 501, 936
TscAI	CASTG	1 cut(s)	1144
TseFI	GTSAC	1 cut(s)	839
TseI	GCWGC	4 cut(s)	767, 866, 1169, 1217
Tsp45I	GTSAC	1 cut(s)	839
TspDTI	ATGAA	7 cut(s)	290, 297, 843, 873, 941, 1199, 1439
TspGWI	ACGGA	3 cut(s)	561, 1170, 1257
TspRI	CASTG	1 cut(s)	1144
Tth111I	GACNNNGTC	1 cut(s)	691
Vha464I	CTTAAG	1 cut(s)	500
VpaK11BI	GGWCC	1 cut(s)	611
XapI	RAATTY	2 cut(s)	463, 1160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	107
XspI	CTAG	3 cut(s)	21, 95, 1166
ZraI	GACGTC	1 cut(s)	26
Zsp2I	ATGCAT	2 cut(s)	237, 444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.