Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0011632

Physical Location & Seq

Genomic Coordinates Forward (+)

6404496 .. 6408527

Gene Structure 4032 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1048300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1503 bp

ATGAAGAAAGGATTGCATTCCCAAGCGGGGAAGCTCCACACAGTACTGCCGTACGAAACGCCACGACTCAGAGACCATTACCTCATGGGCAAGAAATTGGGCCAAGGCCAATTCGGCACCACCTACCTCTGCACCCACAAGCCCACCGGCGCCCAGTTCGCCTGCAAGTCCATCCCCAAGCGCAAGCTCCTCTGCCAGGAGGACTACGACGACGTCTGGAGGGAGATTCAGATCATGCACCACCTCTCCGAGCACCCCACCGTCGTCCAGATCAAGGGAACCTACGAGGACTCCGTCTTCGTGCACCTCGTCATGGAGCTCTGCGCCGGCGGGGAGCTCTTTGATAGGATCGTCCGCAAGGGGCATTACAGCGAGAGGGAGGCGGCCAAGCTCATCAAGACCATCGTCGGGGTGGTGGAGGGCTGCCACTCCCTCGGCGTCATGCACCGCGATCTCAAGCCCGAGAATTTCTTGTTCGACACGCCTTCCGATGACGCCACGCTCAAAGCCACCGATTTCGGCTTGTCCGTCTTCTACAAGCCAGGACAAGTTTTCCAGGACGTCGTTGGGAGTCCCTATTATGTTGCACCTGAGGTGTTGAACAAGATTTATGGACCTGAAGCGGATGTATGGAGTGCTGGTGTGATACTTTACATCCTCTTAAGCGGGGTTCCTCCTTTTTGGGCAGAAACTGAAGCAGGGATATTCAGACAGATTTTACATGGCAAAATCGATTTTGAATCTGAGCCTTGGCCTAATATTTCCAAAAGTGCGAAGGATCTCATGTGTAAGATGCTTGAGAGGAACCCAAAGAAAAGAATATCTGCCCATGAAGTCCTATGTCACCCCTGGATTGTGGACGACAGAGTTGCCCCTGATAAACCACTAGATTCTGCGGTTTTGTCCCGCCTCAAGCAGTTCTCAGCAATGAATAAGCTTAAGAAGATGGCTCTTCGAGTTATAGCAGAAAGACTGTCAGAAGAAGAGATTGGTGGATTAAAAGAGTTGTTCAAAATGATCGACACAGATAATAGCGGGACAATAACATTTGAGGAACTTAAAGAGGGATTGAAAAAAGTGGGCTCGGAGCTAATGGAGTCTGAAATCAAGTCCCTTATGGAAGCAGCTGATATCGACAACAGCGGAACAATAGACTACGGTGAATTTCTTGCTGCTACCTTGCACTTAAACAAGATGGAAAGGGAGGAGAATTTGATTGCAGCCTTTTCCTTTTTTGACAAAGATGGTAGTGGATATATTACCGTCGATGAGCTTCAACAGGCTTGCAAGGAGTTTGGTCTAGGTGACGTTCATCTGGATGAAATTGTCAAAGAGATTGATGAAGACAATGATGGGCGTATCGATTATGGGGAGTTTGCTGCAATGATGAGGAAGGGGGGTGATGGAGGAATTGGGAGGAGCAGAACCATGAGAAACAATTTGAACTTTAACTTGGCTGACGCTTTCGGAATAAAAGATCAGTCTACTTCTACCGGACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009787 GO:0009789 GO:0009931 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023051 GO:0023052 GO:0023056 GO:0032409 GO:0032412 GO:0032879 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080092 GO:0140096 GO:1901016 GO:1901379 GO:1901419 GO:1901421 GO:1901564 GO:1901979 GO:1904062 GO:1905957 GO:1905959 GO:2000241 GO:2001257

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

501

Amino Acids

56.09

Weight (kDa)

5.54

Isoelectric Point (pI)

35.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	29 - 285	2.1e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	29 - 279	4e-27	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	333 - 359	2.1e-08	EF hand domain
EF-hand_7	PF13499	333 - 393	8.3e-13	EF-hand domain pair
EF-hand_6	PF13405	333 - 361	9.4e-08	EF-hand domain
EF-hand_5	PF13202	333 - 355	3.2e-06	EF hand
EF-hand_8	PF13833	345 - 393	2.1e-07	EF-hand domain pair
EF-hand_7	PF13499	402 - 464	1.2e-14	EF-hand domain pair
EF-hand_6	PF13405	405 - 433	6.9e-08	EF-hand domain
EF-hand_1	PF00036	406 - 431	9.2e-07	EF hand domain
EF-hand_5	PF13202	406 - 427	7.8e-06	EF hand
EF-hand_1	PF00036	439 - 465	1e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35670 AT4G09570
fragaria_vesca	FvH4_2g11790
malus_domestica	MD05G1041800.v1.1 MD10G1048300.v1.1
prunus_persica	Prupe.8G057600_v2.0.a1
pyrus_communis	pycom10g03420
rosa_chinensis	RchiOBHm_Chr6g0272011
rosa_laevigata	RLG00000013709
rosa_multiflora	Rmu_co8430291.1_g000001 Rmu_sc0014039.1_g000006
rosa_roxburghii	Rroxscaffold_173G00434970 Rroxscaffold_7G00196830
rosa_rugosa	Rorug06G0064900
rosa_samantha	Rh6BG184900 Rh6CG183500 Rh6DG175000
rosa_wichuraiana	Rw0G003460 Rw6G015690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	216, 564
AccB1I	GGYRCC	2 cut(s)	116, 149
AccI	GTMKAC	1 cut(s)	1484
AccII	CGCG	1 cut(s)	450
AclWI	GGATC	2 cut(s)	356, 786
AcoI	YGGCCR	1 cut(s)	384
AcsI	RAATTY	3 cut(s)	466, 1163, 1210
AcuI	CTGAAG	2 cut(s)	639, 714
AcyI	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
AfaI	GTAC	2 cut(s)	45, 53
AfiI	CCNNNNNNNGG	5 cut(s)	27, 196, 274, 313, 408
AflII	CTTAAG	2 cut(s)	661, 938
AgsI	TTSAA	6 cut(s)	601, 740, 1012, 1072, 1277, 1444
AjnI	CCWGG	4 cut(s)	195, 541, 555, 848
AjuI	GAANNNNNNNTTGG	4 cut(s)	972, 1004, 1436, 1468
AluBI	AGCT	9 cut(s)	34, 187, 319, 337, 391, 937, 1090, 1127, 1273
AluI	AGCT	9 cut(s)	34, 187, 319, 337, 391, 937, 1090, 1127, 1273
Alw21I	GWGCWC	4 cut(s)	255, 306, 321, 339
Alw26I	GTCTC	1 cut(s)	66
Alw44I	GTGCAC	1 cut(s)	302
AlwI	GGATC	2 cut(s)	356, 786
AlwNI	CAGNNNCTG	1 cut(s)	692
Ama87I	CYCGRG	1 cut(s)	461
AoxI	GGCC	4 cut(s)	100, 106, 384, 752
ApaLI	GTGCAC	1 cut(s)	302
ApeKI	GCWGC	5 cut(s)	423, 1124, 1172, 1220, 1379
ApoI	RAATTY	3 cut(s)	466, 1163, 1210
ArsI	GACNNNNNNTTYG	2 cut(s)	281, 313
AspLEI	GCGC	3 cut(s)	152, 183, 326
AspS9I	GGNCC	2 cut(s)	100, 614
AsuHPI	GGTGA	4 cut(s)	836, 1172, 1316, 1412
AvaI	CYCGRG	1 cut(s)	461
AvaII	GGWCC	1 cut(s)	614
AxyI	CCTNAGG	1 cut(s)	591
BaeGI	GKGCMC	1 cut(s)	306
BanI	GGYRCC	2 cut(s)	116, 149
BanII	GRGCYC	3 cut(s)	321, 339, 1085
BbsI	GAAGAC	3 cut(s)	289, 523, 1350
Bbv12I	GWGCWC	4 cut(s)	255, 306, 321, 339
BbvI	GCAGC	5 cut(s)	410, 1136, 1159, 1232, 1366
BccI	CCATC	7 cut(s)	179, 410, 940, 1189, 1238, 1346, 1397
BceAI	ACGGC	1 cut(s)	34
BcgI	CGANNNNNNTGC	2 cut(s)	851, 885
BciT130I	CCWGG	4 cut(s)	197, 543, 557, 850
BcoDI	GTCTC	1 cut(s)	66
BfaI	CTAG	2 cut(s)	887, 1301
BfoI	RGCGCY	1 cut(s)	153
BfrI	CTTAAG	2 cut(s)	661, 938
BglI	GCCNNNNNGGC	1 cut(s)	114
BisI	GCNGC	6 cut(s)	384, 424, 1125, 1173, 1221, 1380
BlsI	GCNGC	6 cut(s)	385, 425, 1126, 1174, 1222, 1381
BmcAI	AGTACT	1 cut(s)	45
Bme1390I	CCNGG	4 cut(s)	197, 543, 557, 850
Bme18I	GGWCC	1 cut(s)	614
BmeT110I	CYCGRG	1 cut(s)	461
BmgT120I	GGNCC	2 cut(s)	100, 614
BmiI	GGNNCC	5 cut(s)	118, 151, 280, 672, 806
BmrFI	CCNGG	4 cut(s)	197, 543, 557, 850
BmrI	ACTGGG	1 cut(s)	148
BmsI	GCATC	1 cut(s)	783
BmuI	ACTGGG	1 cut(s)	148
BoxI	GACNNNNGTC	1 cut(s)	404
BpiI	GAAGAC	3 cut(s)	289, 523, 1350
BpmI	CTGGAG	1 cut(s)	238
BpuEI	CTTGAG	3 cut(s)	440, 818, 896
Bsa29I	ATCGAT	2 cut(s)	732, 1362
BsaBI	GATNNNNATC	1 cut(s)	230
BsaHI	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
BsaI	GGTCTC	1 cut(s)	66
BsaJI	CCNNGG	4 cut(s)	103, 433, 749, 848
BsaWI	WCCGGW	1 cut(s)	1493
BsaXI	ACNNNNNCTCC	6 cut(s)	230, 260, 275, 305, 625, 655
Bsc4I	CCNNNNNNNGG	5 cut(s)	27, 196, 274, 313, 408
Bse118I	RCCGGY	2 cut(s)	146, 326
Bse1I	ACTGG	1 cut(s)	154
Bse21I	CCTNAGG	1 cut(s)	591
Bse3DI	GCAATG	2 cut(s)	933, 1389
Bse8I	GATNNNNATC	1 cut(s)	230
BseBI	CCWGG	4 cut(s)	197, 543, 557, 850
BseCI	ATCGAT	2 cut(s)	732, 1362
BseDI	CCNNGG	4 cut(s)	103, 433, 749, 848
BseGI	GGATG	4 cut(s)	171, 631, 654, 1324
BseJI	GATNNNNATC	1 cut(s)	230
BseLI	CCNNNNNNNGG	5 cut(s)	27, 196, 274, 313, 408
BseMI	GCAATG	2 cut(s)	933, 1389
BseMII	CTCAG	4 cut(s)	82, 582, 735, 936
BseNI	ACTGG	1 cut(s)	154
BseRI	GAGGAG	3 cut(s)	179, 1220, 1432
BseSI	GKGCMC	1 cut(s)	306
BseXI	GCAGC	5 cut(s)	410, 1136, 1159, 1232, 1366
BsgI	GTGCAG	1 cut(s)	115
Bsh1236I	CGCG	1 cut(s)	450
BshFI	GGCC	4 cut(s)	102, 108, 386, 754
BshNI	GGYRCC	2 cut(s)	116, 149
BshVI	ATCGAT	2 cut(s)	732, 1362
BsiHKAI	GWGCWC	4 cut(s)	255, 306, 321, 339
BsiHKCI	CYCGRG	1 cut(s)	461
BsiSI	CCGG	3 cut(s)	147, 327, 1494
BsiWI	CGTACG	1 cut(s)	51
BslFI	GGGAC	4 cut(s)	558, 889, 1051, 1096
BslI	CCNNNNNNNGG	5 cut(s)	27, 196, 274, 313, 408
BsmAI	GTCTC	1 cut(s)	66
BsmFI	GGGAC	4 cut(s)	558, 889, 1051, 1096
BsmI	GAATGC	1 cut(s)	16
BsnI	GGCC	4 cut(s)	102, 108, 386, 754
Bso31I	GGTCTC	1 cut(s)	66
BsoBI	CYCGRG	1 cut(s)	461
Bsp1286I	GDGCHC	5 cut(s)	255, 306, 321, 339, 1085
Bsp143I	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
BspANI	GGCC	4 cut(s)	102, 108, 386, 754
BspCNI	CTCAG	4 cut(s)	81, 583, 736, 935
BspDI	ATCGAT	2 cut(s)	732, 1362
BspFNI	CGCG	1 cut(s)	450
BspLI	GGNNCC	5 cut(s)	118, 151, 280, 672, 806
BspPI	GGATC	2 cut(s)	356, 786
BspQI	GCTCTTC	1 cut(s)	957
BspT107I	GGYRCC	2 cut(s)	116, 149
BspTI	CTTAAG	2 cut(s)	661, 938
BspTNI	GGTCTC	1 cut(s)	66
BsrDI	GCAATG	2 cut(s)	933, 1389
BsrFI	RCCGGY	2 cut(s)	146, 326
BsrI	ACTGG	1 cut(s)	154
BssAI	RCCGGY	2 cut(s)	146, 326
BssECI	CCNNGG	4 cut(s)	103, 433, 749, 848
BssMI	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
BssNI	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
BssT1I	CCWWGG	2 cut(s)	103, 749
Bst2UI	CCWGG	4 cut(s)	197, 543, 557, 850
Bst4CI	ACNGT	5 cut(s)	43, 262, 975, 1160, 1264
Bst6I	CTCTTC	2 cut(s)	957, 978
BstACI	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
BstAFI	CTTAAG	2 cut(s)	661, 938
BstC8I	GCNNGC	4 cut(s)	163, 185, 328, 1285
BstDEI	CTNAG	4 cut(s)	68, 591, 744, 922
BstENI	CCTNNNNNAGG	1 cut(s)	194
BstF5I	GGATG	4 cut(s)	171, 631, 654, 1324
BstFNI	CGCG	1 cut(s)	450
BstH2I	RGCGCY	1 cut(s)	153
BstHHI	GCGC	3 cut(s)	152, 183, 326
BstKTI	GATC	7 cut(s)	234, 273, 351, 454, 781, 1020, 1480
BstMAI	GTCTC	1 cut(s)	66
BstMBI	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
BstMWI	GCNNNNNNNGC	2 cut(s)	114, 158
BstNI	CCWGG	4 cut(s)	197, 543, 557, 850
BstPAI	GACNNNNGTC	1 cut(s)	404
BstSCI	CCNGG	4 cut(s)	195, 541, 555, 848
BstSLI	GKGCMC	1 cut(s)	306
BstUI	CGCG	1 cut(s)	450
BstV1I	GCAGC	5 cut(s)	410, 1136, 1159, 1232, 1366
BstV2I	GAAGAC	3 cut(s)	289, 523, 1350
BstX2I	RGATCY	1 cut(s)	778
BstYI	RGATCY	1 cut(s)	778
Bsu15I	ATCGAT	2 cut(s)	732, 1362
Bsu36I	CCTNAGG	1 cut(s)	591
BsuRI	GGCC	4 cut(s)	102, 108, 386, 754
BsuTUI	ATCGAT	2 cut(s)	732, 1362
BtsCI	GGATG	4 cut(s)	171, 631, 654, 1324
Cac8I	GCNNGC	4 cut(s)	163, 185, 328, 1285
CaiI	CAGNNNCTG	1 cut(s)	692
CfoI	GCGC	3 cut(s)	152, 183, 326
Cfr10I	RCCGGY	2 cut(s)	146, 326
Cfr13I	GGNCC	2 cut(s)	100, 614
ClaI	ATCGAT	2 cut(s)	732, 1362
CseI	GACGC	3 cut(s)	427, 503, 1469
Csp6I	GTAC	2 cut(s)	44, 52
CviAII	CATG	8 cut(s)	85, 235, 313, 442, 722, 784, 830, 1429
CviQI	GTAC	2 cut(s)	44, 52
DdeI	CTNAG	4 cut(s)	68, 591, 744, 922
DinI	GGCGCC	1 cut(s)	151
DpnI	GATC	7 cut(s)	233, 272, 350, 453, 780, 1019, 1479
DpnII	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
EaeI	YGGCCR	1 cut(s)	384
Eam1104I	CTCTTC	2 cut(s)	957, 978
EarI	CTCTTC	2 cut(s)	957, 978
Ecl136II	GAGCTC	2 cut(s)	319, 337
Eco130I	CCWWGG	2 cut(s)	103, 749
Eco24I	GRGCYC	3 cut(s)	321, 339, 1085
Eco31I	GGTCTC	1 cut(s)	66
Eco32I	GATATC	1 cut(s)	1132
Eco47I	GGWCC	1 cut(s)	614
Eco53kI	GAGCTC	2 cut(s)	319, 337
Eco57I	CTGAAG	2 cut(s)	639, 714
Eco81I	CCTNAGG	1 cut(s)	591
Eco88I	CYCGRG	1 cut(s)	461
EcoICRI	GAGCTC	2 cut(s)	319, 337
EcoNI	CCTNNNNNAGG	1 cut(s)	194
EcoRII	CCWGG	4 cut(s)	195, 541, 555, 848
EcoRV	GATATC	1 cut(s)	1132
EcoT14I	CCWWGG	2 cut(s)	103, 749
EcoT38I	GRGCYC	3 cut(s)	321, 339, 1085
EgeI	GGCGCC	1 cut(s)	151
EheI	GGCGCC	1 cut(s)	151
ErhI	CCWWGG	2 cut(s)	103, 749
FaeI	CATG	8 cut(s)	88, 238, 316, 445, 725, 787, 833, 1432
FaqI	GGGAC	4 cut(s)	558, 889, 1051, 1096
FatI	CATG	8 cut(s)	84, 234, 312, 441, 721, 783, 829, 1428
FauI	CCCGC	5 cut(s)	19, 323, 659, 914, 1028
FblI	GTMKAC	1 cut(s)	1484
Fnu4HI	GCNGC	6 cut(s)	384, 424, 1125, 1173, 1221, 1380
FokI	GGATG	4 cut(s)	158, 638, 641, 1331
FriOI	GRGCYC	3 cut(s)	321, 339, 1085
Fsp4HI	GCNGC	6 cut(s)	384, 424, 1125, 1173, 1221, 1380
FspBI	CTAG	2 cut(s)	887, 1301
GlaI	GCGC	3 cut(s)	151, 182, 325
GluI	GCNGC	6 cut(s)	384, 424, 1125, 1173, 1221, 1380
GsuI	CTGGAG	1 cut(s)	238
HaeII	RGCGCY	1 cut(s)	153
HaeIII	GGCC	4 cut(s)	102, 108, 386, 754
HapII	CCGG	3 cut(s)	147, 327, 1494
HgaI	GACGC	3 cut(s)	427, 503, 1469
HhaI	GCGC	3 cut(s)	152, 183, 326
Hin1I	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
Hin1II	CATG	8 cut(s)	88, 238, 316, 445, 725, 787, 833, 1432
Hin6I	GCGC	3 cut(s)	150, 181, 324
HinP1I	GCGC	3 cut(s)	150, 181, 324
HindIII	AAGCTT	1 cut(s)	935
HinfI	GANTC	7 cut(s)	66, 226, 290, 571, 740, 890, 1097
HpaII	CCGG	3 cut(s)	147, 327, 1494
HphI	GGTGA	4 cut(s)	836, 1172, 1316, 1412
Hpy166II	GTNNAC	3 cut(s)	304, 859, 1485
Hpy188III	TCNNGA	4 cut(s)	217, 268, 397, 1316
Hpy8I	GTNNAC	3 cut(s)	304, 859, 1485
Hpy99I	CGWCG	6 cut(s)	212, 215, 266, 410, 566, 1268
HpyAV	CCTTC	3 cut(s)	495, 769, 1387
HpyCH4III	ACNGT	5 cut(s)	43, 262, 975, 1160, 1264
HpyCH4IV	ACGT	3 cut(s)	213, 561, 1308
HpyF10VI	GCNNNNNNNGC	2 cut(s)	114, 158
HpyF3I	CTNAG	4 cut(s)	68, 591, 744, 922
HpySE526I	ACGT	3 cut(s)	213, 561, 1308
Hsp92I	GRCGYC	5 cut(s)	150, 213, 438, 495, 561
Hsp92II	CATG	8 cut(s)	88, 238, 316, 445, 725, 787, 833, 1432
HspAI	GCGC	3 cut(s)	150, 181, 324
KasI	GGCGCC	1 cut(s)	149
KroI	GCCGGC	1 cut(s)	326
KroNI	GCCGGC	1 cut(s)	328
Kzo9I	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
LguI	GCTCTTC	1 cut(s)	957
LmnI	GCTCC	6 cut(s)	39, 192, 316, 334, 1087, 1419
Lsp1109I	GCAGC	5 cut(s)	410, 1136, 1159, 1232, 1366
LweI	GCATC	1 cut(s)	783
MaeI	CTAG	2 cut(s)	887, 1301
MaeII	ACGT	3 cut(s)	213, 561, 1308
MaeIII	GTNAC	2 cut(s)	842, 1304
MalI	GATC	7 cut(s)	233, 272, 350, 453, 780, 1019, 1479
MboI	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
MboII	GAAGA	8 cut(s)	16, 289, 523, 944, 955, 992, 995, 1355
MflI	RGATCY	1 cut(s)	778
MhlI	GDGCHC	5 cut(s)	255, 306, 321, 339, 1085
MluCI	AATT	8 cut(s)	95, 110, 466, 1163, 1210, 1323, 1410, 1438
Mly113I	GGCGCC	1 cut(s)	150
MlyI	GAGTC	4 cut(s)	60, 284, 580, 1106
MreI	CGCCGGCG	1 cut(s)	326
MroNI	GCCGGC	1 cut(s)	326
MseI	TTAA	6 cut(s)	662, 939, 998, 1059, 1187, 1449
MslI	CAYNNNNRTG	1 cut(s)	1317
MspA1I	CMGCKG	2 cut(s)	1127, 1143
MspCI	CTTAAG	2 cut(s)	661, 938
MspI	CCGG	3 cut(s)	147, 327, 1494
MspR9I	CCNGG	4 cut(s)	197, 543, 557, 850
Mva1269I	GAATGC	1 cut(s)	16
MvaI	CCWGG	4 cut(s)	197, 543, 557, 850
MvnI	CGCG	1 cut(s)	450
MwoI	GCNNNNNNNGC	2 cut(s)	114, 158
NaeI	GCCGGC	1 cut(s)	328
NarI	GGCGCC	1 cut(s)	150
NdeII	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
NgoMIV	GCCGGC	1 cut(s)	326
NlaIII	CATG	8 cut(s)	88, 238, 316, 445, 725, 787, 833, 1432
NlaIV	GGNNCC	5 cut(s)	118, 151, 280, 672, 806
NmeAIII	GCCGAG	1 cut(s)	414
NmuCI	GTSAC	2 cut(s)	842, 1304
PciSI	GCTCTTC	1 cut(s)	957
PcsI	WCGNNNNNNNCGW	3 cut(s)	291, 306, 525
PctI	GAATGC	1 cut(s)	16
PdiI	GCCGGC	1 cut(s)	328
PfeI	GAWTC	3 cut(s)	226, 740, 890
Pfl23II	CGTACG	1 cut(s)	51
PflFI	GACNNNGTC	2 cut(s)	212, 293
PfoI	TCCNGGA	1 cut(s)	555
PkrI	GCNGC	6 cut(s)	385, 425, 1126, 1174, 1222, 1381
PleI	GAGTC	4 cut(s)	60, 284, 579, 1105
PluTI	GGCGCC	1 cut(s)	153
PpsI	GAGTC	4 cut(s)	60, 284, 579, 1105
PshAI	GACNNNNGTC	1 cut(s)	404
Psp124BI	GAGCTC	2 cut(s)	321, 339
Psp6I	CCWGG	4 cut(s)	195, 541, 555, 848
PspGI	CCWGG	4 cut(s)	195, 541, 555, 848
PspLI	CGTACG	1 cut(s)	51
PspN4I	GGNNCC	5 cut(s)	118, 151, 280, 672, 806
PspPI	GGNCC	2 cut(s)	100, 614
PstNI	CAGNNNCTG	1 cut(s)	692
PsuI	RGATCY	1 cut(s)	778
PsyI	GACNNNGTC	2 cut(s)	212, 293
PvuII	CAGCTG	1 cut(s)	1127
RsaI	GTAC	2 cut(s)	45, 53
RsaNI	GTAC	2 cut(s)	44, 52
RseI	CAYNNNNRTG	1 cut(s)	1317
SacI	GAGCTC	2 cut(s)	321, 339
SapI	GCTCTTC	1 cut(s)	957
SaqAI	TTAA	6 cut(s)	662, 939, 998, 1059, 1187, 1449
SatI	GCNGC	6 cut(s)	384, 424, 1125, 1173, 1221, 1380
Sau3AI	GATC	7 cut(s)	231, 270, 348, 451, 778, 1017, 1477
Sau96I	GGNCC	2 cut(s)	100, 614
ScaI	AGTACT	1 cut(s)	45
SchI	GAGTC	4 cut(s)	60, 284, 580, 1106
ScrFI	CCNGG	4 cut(s)	197, 543, 557, 850
SduI	GDGCHC	5 cut(s)	255, 306, 321, 339, 1085
SfaNI	GCATC	1 cut(s)	783
SfoI	GGCGCC	1 cut(s)	151
SgrAI	CRCCGGYG	2 cut(s)	146, 326
SinI	GGWCC	1 cut(s)	614
SmiMI	CAYNNNNRTG	1 cut(s)	1317
SmlI	CTYRAG	5 cut(s)	455, 661, 797, 911, 938
SmoI	CTYRAG	5 cut(s)	455, 661, 797, 911, 938
Sse9I	AATT	8 cut(s)	95, 110, 466, 1163, 1210, 1323, 1410, 1438
SspDI	GGCGCC	1 cut(s)	149
SspI	AATATT	1 cut(s)	760
SspMI	CTAG	2 cut(s)	887, 1301
SstI	GAGCTC	2 cut(s)	321, 339
StyD4I	CCNGG	4 cut(s)	195, 541, 555, 848
StyI	CCWWGG	2 cut(s)	103, 749
TaaI	ACNGT	5 cut(s)	43, 262, 975, 1160, 1264
TaiI	ACGT	3 cut(s)	216, 564, 1311
TaqI	TCGA	7 cut(s)	477, 732, 955, 1020, 1134, 1266, 1362
TasI	AATT	8 cut(s)	95, 110, 466, 1163, 1210, 1323, 1410, 1438
TatI	WGTACW	1 cut(s)	43
TauI	GCSGC	1 cut(s)	386
TfiI	GAWTC	3 cut(s)	226, 740, 890
Tru1I	TTAA	6 cut(s)	662, 939, 998, 1059, 1187, 1449
Tru9I	TTAA	6 cut(s)	662, 939, 998, 1059, 1187, 1449
TseFI	GTSAC	2 cut(s)	842, 1304
TseI	GCWGC	5 cut(s)	423, 1124, 1172, 1220, 1379
Tsp45I	GTSAC	2 cut(s)	842, 1304
TspDTI	ATGAA	6 cut(s)	17, 846, 944, 1301, 1335, 1356
TspGWI	ACGGA	2 cut(s)	283, 517
Tth111I	GACNNNGTC	2 cut(s)	212, 293
Vha464I	CTTAAG	2 cut(s)	661, 938
VneI	GTGCAC	1 cut(s)	302
VpaK11BI	GGWCC	1 cut(s)	614
XagI	CCTNNNNNAGG	1 cut(s)	194
XapI	RAATTY	3 cut(s)	466, 1163, 1210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	409, 563
XmiI	GTMKAC	1 cut(s)	1484
XspI	CTAG	2 cut(s)	887, 1301
ZraI	GACGTC	2 cut(s)	214, 562
ZrmI	AGTACT	1 cut(s)	45

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.