Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011632

Physical Location & Seq

Genomic Coordinates Forward (+)

7036742 .. 7040731

Gene Structure 3990 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1041800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGAAGAAAGGATCGCATTCAGTATTGCCGTACGAAACGCCGCGGCTGAGAGACCATTACCTGATGGGCAAGAAATTGGGCCAAGGACAATTCGGCACCACCTACCTCTGCACCCACAAGCCCACCGGCGCCCACTACGCCTGCAAGTCCATCCCCAAGCGCAAGCTCCTCTGCCAGGAGGACTACGACGACGTCTGGAGGGAGATTCAAATCATGCACCACCTCTCCGAGCACCCCAGCGTCGTCCAGATCAAGGGCACCTACGAGGACTCCGTCTTCGTGCACCTCGTCATGGAGCTCTGTGCCGGCGGCGAGCTGTTTGATAGGATCGTCAAGAAGGGGCATTACAGCGAGAGGGAGGCGGCCAAGCTCATCAAGACCATCGTTGGGGTGGTGGAGGGCTGCCACTCCCTCGGTGTCATGCACCGCGATCTCAAGCCGGAGAATTTCTTGTTCGACACGCCTTCGGATGATGCCACGCTCAAAGCCACCGATTTCGGCTTGTCCGTCTTCTATAAGCCAGGACAATATTTCTATGACATAGTTGGGAGTCCCTATTATGTTGCACCTGAGGTGTTGTCCAAGATTTATGGACCTGAAGTGGATGTATGGAGTGCTGGTGTGATACTTTACATCCTCTTAAGCGGGGTTCCTCCTTTTTGGGCAGAAACTGAATCAGGGATCTTCAGACAGATTTTACATGGCAAAATAGATTTTGAATCTGAGCCGTGGCCTAATATTTCAGAAAGTGCGAAAGATCTCATGTATAAGATGCTTGAGAGGCACCCGAAGAAAAGAATCTCTGCCCATGAAGTCCTATGTCACCCCTGGATTGTAGACGACAGAGTTGCCCCAGATAAACCACTAGATTCTGCGGTTCTGTCCCGCCTCAAGCAGTTCTCAGCAATGAATAAGCTTAAGAAGATGGCACTTCGTGTGATAGCGGAAAGACTGTCAGAAGAAGAGATTGGAGGTTTAAAAGAGCTGTTCAAAATGATTGACACAGATAGTAGCGGGACAATAACATTTGAGGAACTTAAAGAAGGATTGAAAAAAGTGGGCTCGGAGCTAATGGAGTCTGAAATCAAGTCCCTTATGGAAGCAGCTGATATAGACAACAGCGGAACAATAGACTATGGCGAATTTCTTGCTGCTACCTTGCACTTAAACAAGATGGAAAGAGAGGAGAATTTGATTGCAGCCTTTTCCTTTTTTGACAAAGATGGTAGTGGATATATTACTGTCGACGAGCTTCAACAGGCTTGCAAGGAGTTTGGTCTAGGTGACGTTCATCTGGATGAAATTGTCAAAGAGATTGATGAAGACAATGATGGGCGTATCGATTATGGGGAGTTTGCTGCAATGATGAGGAAGGGGGGTGATGGAGGAATTGGGAGGAGCAGAACCATGAGAAACAATTTGAACTTTAATTTGGGTGACGCTTTCGGAATAAAAGATCAGTCTACGTCTTCGTCTGCCGGAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009787 GO:0009789 GO:0009931 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023051 GO:0023052 GO:0023056 GO:0032409 GO:0032412 GO:0032879 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045595 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080092 GO:0140096 GO:1901016 GO:1901379 GO:1901419 GO:1901421 GO:1901564 GO:1901979 GO:1904062 GO:1905957 GO:1905959 GO:2000241 GO:2001257

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

496

Amino Acids

55.63

Weight (kDa)

5.37

Isoelectric Point (pI)

40.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	22 - 278	9.1e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	22 - 272	2.6e-26	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	326 - 352	1.3e-07	EF hand domain
EF-hand_7	PF13499	326 - 386	4.8e-12	EF-hand domain pair
EF-hand_6	PF13405	326 - 354	4.7e-07	EF-hand domain
EF-hand_5	PF13202	326 - 348	8.2e-06	EF hand
EF-hand_8	PF13833	337 - 386	8.4e-08	EF-hand domain pair
EF-hand_7	PF13499	395 - 457	1.2e-14	EF-hand domain pair
EF-hand_6	PF13405	398 - 426	6.8e-08	EF-hand domain
EF-hand_1	PF00036	399 - 424	9.1e-07	EF hand domain
EF-hand_5	PF13202	399 - 420	7.7e-06	EF hand
EF-hand_1	PF00036	432 - 458	1e-06	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35670 AT4G09570
fragaria_vesca	FvH4_2g11790
malus_domestica	MD05G1041800.v1.1 MD10G1048300.v1.1
prunus_persica	Prupe.8G057600_v2.0.a1
pyrus_communis	pycom10g03420
rosa_chinensis	RchiOBHm_Chr6g0272011
rosa_laevigata	RLG00000013709
rosa_multiflora	Rmu_co8430291.1_g000001 Rmu_sc0014039.1_g000006
rosa_roxburghii	Rroxscaffold_173G00434970 Rroxscaffold_7G00196830
rosa_rugosa	Rorug06G0064900
rosa_samantha	Rh6BG184900 Rh6CG183500 Rh6DG175000
rosa_wichuraiana	Rw0G003460 Rw6G015690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	195
AccB1I	GGYRCC	4 cut(s)	95, 128, 257, 783
AccI	GTMKAC	3 cut(s)	837, 1245, 1463
AccII	CGCG	2 cut(s)	43, 429
AclWI	GGATC	3 cut(s)	19, 335, 689
AcoI	YGGCCR	1 cut(s)	363
AcsI	RAATTY	3 cut(s)	445, 1142, 1189
AcuI	CTGAAG	2 cut(s)	618, 670
AcyI	GRCGYC	2 cut(s)	129, 192
AdeI	CACNNNGTG	1 cut(s)	935
AfaI	GTAC	1 cut(s)	32
AfiI	CCNNNNNNNGG	4 cut(s)	175, 253, 292, 387
AflII	CTTAAG	2 cut(s)	640, 917
AgsI	TTSAA	6 cut(s)	209, 719, 991, 1051, 1256, 1423
AjnI	CCWGG	3 cut(s)	174, 520, 827
AjuI	GAANNNNNNNTTGG	4 cut(s)	951, 983, 1415, 1447
AluBI	AGCT	9 cut(s)	166, 298, 316, 370, 916, 985, 1069, 1106, 1252
AluI	AGCT	9 cut(s)	166, 298, 316, 370, 916, 985, 1069, 1106, 1252
Alw21I	GWGCWC	3 cut(s)	234, 285, 300
Alw26I	GTCTC	1 cut(s)	45
Alw44I	GTGCAC	1 cut(s)	281
AlwI	GGATC	3 cut(s)	19, 335, 689
AlwNI	CAGNNNCTG	1 cut(s)	671
AoxI	GGCC	3 cut(s)	79, 363, 731
ApaLI	GTGCAC	1 cut(s)	281
ApeKI	GCWGC	5 cut(s)	402, 1103, 1151, 1199, 1358
ApoI	RAATTY	3 cut(s)	445, 1142, 1189
ArsI	GACNNNNNNTTYG	2 cut(s)	260, 292
AspLEI	GCGC	2 cut(s)	131, 162
AspS9I	GGNCC	2 cut(s)	79, 593
AsuHPI	GGTGA	4 cut(s)	815, 1295, 1391, 1448
AvaII	GGWCC	1 cut(s)	593
AxyI	CCTNAGG	1 cut(s)	570
BaeGI	GKGCMC	2 cut(s)	260, 285
BanI	GGYRCC	4 cut(s)	95, 128, 257, 783
BanII	GRGCYC	2 cut(s)	300, 1064
BarI	GAAGNNNNNNTAC	2 cut(s)	329, 361
BbsI	GAAGAC	4 cut(s)	268, 502, 1329, 1461
Bbv12I	GWGCWC	3 cut(s)	234, 285, 300
BbvI	GCAGC	5 cut(s)	389, 1115, 1138, 1211, 1345
BccI	CCATC	8 cut(s)	58, 158, 389, 919, 1168, 1217, 1325, 1376
BceAI	ACGGC	2 cut(s)	13, 712
BcgI	CGANNNNNNTGC	4 cut(s)	830, 864, 1130, 1164
BciT130I	CCWGG	3 cut(s)	176, 522, 829
BcoDI	GTCTC	1 cut(s)	45
BfaI	CTAG	2 cut(s)	866, 1280
BfoI	RGCGCY	1 cut(s)	132
BfrI	CTTAAG	2 cut(s)	640, 917
BglII	AGATCT	1 cut(s)	757
BisI	GCNGC	9 cut(s)	41, 44, 310, 363, 403, 1104, 1152, 1200, 1359
BlsI	GCNGC	9 cut(s)	42, 45, 311, 364, 404, 1105, 1153, 1201, 1360
Bme1390I	CCNGG	3 cut(s)	176, 522, 829
Bme18I	GGWCC	1 cut(s)	593
BmgT120I	GGNCC	2 cut(s)	79, 593
BmiI	GGNNCC	5 cut(s)	97, 130, 259, 651, 785
BmrFI	CCNGG	3 cut(s)	176, 522, 829
BmsI	GCATC	2 cut(s)	463, 762
BpiI	GAAGAC	4 cut(s)	268, 502, 1329, 1461
BpmI	CTGGAG	1 cut(s)	217
BpuEI	CTTGAG	3 cut(s)	419, 797, 875
Bsa29I	ATCGAT	1 cut(s)	1341
BsaBI	GATNNNNATC	1 cut(s)	209
BsaHI	GRCGYC	2 cut(s)	129, 192
BsaI	GGTCTC	1 cut(s)	45
BsaJI	CCNNGG	5 cut(s)	41, 82, 412, 728, 827
BsaXI	ACNNNNNCTCC	6 cut(s)	209, 239, 254, 284, 604, 634
Bsc4I	CCNNNNNNNGG	4 cut(s)	175, 253, 292, 387
Bse118I	RCCGGY	2 cut(s)	125, 305
Bse21I	CCTNAGG	1 cut(s)	570
Bse3DI	GCAATG	2 cut(s)	912, 1368
Bse8I	GATNNNNATC	1 cut(s)	209
BseBI	CCWGG	3 cut(s)	176, 522, 829
BseCI	ATCGAT	1 cut(s)	1341
BseDI	CCNNGG	5 cut(s)	41, 82, 412, 728, 827
BseGI	GGATG	5 cut(s)	150, 475, 610, 633, 1303
BseJI	GATNNNNATC	1 cut(s)	209
BseLI	CCNNNNNNNGG	4 cut(s)	175, 253, 292, 387
BseMI	GCAATG	2 cut(s)	912, 1368
BseMII	CTCAG	4 cut(s)	38, 561, 714, 915
BseRI	GAGGAG	3 cut(s)	158, 1199, 1411
BseSI	GKGCMC	2 cut(s)	260, 285
BseXI	GCAGC	5 cut(s)	389, 1115, 1138, 1211, 1345
BseYI	CCCAGC	1 cut(s)	236
BsgI	GTGCAG	1 cut(s)	94
Bsh1236I	CGCG	2 cut(s)	43, 429
BshFI	GGCC	3 cut(s)	81, 365, 733
BshNI	GGYRCC	4 cut(s)	95, 128, 257, 783
BshVI	ATCGAT	1 cut(s)	1341
BsiHKAI	GWGCWC	3 cut(s)	234, 285, 300
BsiSI	CCGG	4 cut(s)	126, 306, 440, 1479
BsiWI	CGTACG	1 cut(s)	30
BslFI	GGGAC	4 cut(s)	537, 868, 1030, 1075
BslI	CCNNNNNNNGG	4 cut(s)	175, 253, 292, 387
BsmAI	GTCTC	1 cut(s)	45
BsmFI	GGGAC	4 cut(s)	537, 868, 1030, 1075
BsmI	GAATGC	1 cut(s)	16
BsnI	GGCC	3 cut(s)	81, 365, 733
Bso31I	GGTCTC	1 cut(s)	45
Bsp1286I	GDGCHC	5 cut(s)	234, 260, 285, 300, 1064
Bsp143I	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
BspANI	GGCC	3 cut(s)	81, 365, 733
BspCNI	CTCAG	4 cut(s)	39, 562, 715, 914
BspDI	ATCGAT	1 cut(s)	1341
BspFNI	CGCG	2 cut(s)	43, 429
BspLI	GGNNCC	5 cut(s)	97, 130, 259, 651, 785
BspPI	GGATC	3 cut(s)	19, 335, 689
BspT107I	GGYRCC	4 cut(s)	95, 128, 257, 783
BspTI	CTTAAG	2 cut(s)	640, 917
BspTNI	GGTCTC	1 cut(s)	45
BsrDI	GCAATG	2 cut(s)	912, 1368
BsrFI	RCCGGY	2 cut(s)	125, 305
BssAI	RCCGGY	2 cut(s)	125, 305
BssECI	CCNNGG	5 cut(s)	41, 82, 412, 728, 827
BssMI	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
BssNI	GRCGYC	2 cut(s)	129, 192
BssT1I	CCWWGG	1 cut(s)	82
Bst2UI	CCWGG	3 cut(s)	176, 522, 829
Bst4CI	ACNGT	2 cut(s)	954, 1243
Bst6I	CTCTTC	1 cut(s)	957
BstACI	GRCGYC	2 cut(s)	129, 192
BstAFI	CTTAAG	2 cut(s)	640, 917
BstC8I	GCNNGC	5 cut(s)	142, 164, 307, 314, 1264
BstDEI	CTNAG	4 cut(s)	47, 570, 723, 901
BstDSI	CCRYGG	2 cut(s)	41, 728
BstENI	CCTNNNNNAGG	1 cut(s)	173
BstF5I	GGATG	5 cut(s)	150, 475, 610, 633, 1303
BstFNI	CGCG	2 cut(s)	43, 429
BstH2I	RGCGCY	1 cut(s)	132
BstHHI	GCGC	2 cut(s)	131, 162
BstKTI	GATC	7 cut(s)	14, 252, 330, 433, 684, 760, 1459
BstMAI	GTCTC	1 cut(s)	45
BstMBI	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
BstMWI	GCNNNNNNNGC	2 cut(s)	137, 781
BstNI	CCWGG	3 cut(s)	176, 522, 829
BstSCI	CCNGG	3 cut(s)	174, 520, 827
BstSLI	GKGCMC	2 cut(s)	260, 285
BstUI	CGCG	2 cut(s)	43, 429
BstV1I	GCAGC	5 cut(s)	389, 1115, 1138, 1211, 1345
BstV2I	GAAGAC	4 cut(s)	268, 502, 1329, 1461
BstX2I	RGATCY	2 cut(s)	681, 757
BstYI	RGATCY	2 cut(s)	681, 757
Bsu15I	ATCGAT	1 cut(s)	1341
Bsu36I	CCTNAGG	1 cut(s)	570
BsuRI	GGCC	3 cut(s)	81, 365, 733
BsuTUI	ATCGAT	1 cut(s)	1341
BtgI	CCRYGG	2 cut(s)	41, 728
BtsCI	GGATG	5 cut(s)	150, 475, 610, 633, 1303
Cac8I	GCNNGC	5 cut(s)	142, 164, 307, 314, 1264
CaiI	CAGNNNCTG	1 cut(s)	671
CfoI	GCGC	2 cut(s)	131, 162
Cfr10I	RCCGGY	2 cut(s)	125, 305
Cfr13I	GGNCC	2 cut(s)	79, 593
Cfr42I	CCGCGG	1 cut(s)	44
ClaI	ATCGAT	1 cut(s)	1341
CseI	GACGC	2 cut(s)	229, 1448
Csp6I	GTAC	1 cut(s)	31
CviAII	CATG	7 cut(s)	214, 292, 421, 701, 763, 809, 1408
CviQI	GTAC	1 cut(s)	31
DdeI	CTNAG	4 cut(s)	47, 570, 723, 901
DinI	GGCGCC	1 cut(s)	130
DpnI	GATC	7 cut(s)	13, 251, 329, 432, 683, 759, 1458
DpnII	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
DraI	TTTAAA	1 cut(s)	978
DraIII	CACNNNGTG	1 cut(s)	935
EaeI	YGGCCR	1 cut(s)	363
Eam1104I	CTCTTC	1 cut(s)	957
EarI	CTCTTC	1 cut(s)	957
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	82
Eco24I	GRGCYC	2 cut(s)	300, 1064
Eco31I	GGTCTC	1 cut(s)	45
Eco47I	GGWCC	1 cut(s)	593
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	2 cut(s)	618, 670
Eco81I	CCTNAGG	1 cut(s)	570
EcoICRI	GAGCTC	1 cut(s)	298
EcoNI	CCTNNNNNAGG	1 cut(s)	173
EcoRII	CCWGG	3 cut(s)	174, 520, 827
EcoT14I	CCWWGG	1 cut(s)	82
EcoT38I	GRGCYC	2 cut(s)	300, 1064
EgeI	GGCGCC	1 cut(s)	130
EheI	GGCGCC	1 cut(s)	130
ErhI	CCWWGG	1 cut(s)	82
FaeI	CATG	7 cut(s)	217, 295, 424, 704, 766, 812, 1411
FaqI	GGGAC	4 cut(s)	537, 868, 1030, 1075
FatI	CATG	7 cut(s)	213, 291, 420, 700, 762, 808, 1407
FauI	CCCGC	3 cut(s)	638, 893, 1007
FblI	GTMKAC	3 cut(s)	837, 1245, 1463
Fnu4HI	GCNGC	9 cut(s)	41, 44, 310, 363, 403, 1104, 1152, 1200, 1359
FokI	GGATG	5 cut(s)	137, 482, 617, 620, 1310
FriOI	GRGCYC	2 cut(s)	300, 1064
Fsp4HI	GCNGC	9 cut(s)	41, 44, 310, 363, 403, 1104, 1152, 1200, 1359
FspBI	CTAG	2 cut(s)	866, 1280
GlaI	GCGC	2 cut(s)	130, 161
GluI	GCNGC	9 cut(s)	41, 44, 310, 363, 403, 1104, 1152, 1200, 1359
GsaI	CCCAGC	1 cut(s)	240
GsuI	CTGGAG	1 cut(s)	217
HaeII	RGCGCY	1 cut(s)	132
HaeIII	GGCC	3 cut(s)	81, 365, 733
HapII	CCGG	4 cut(s)	126, 306, 440, 1479
HgaI	GACGC	2 cut(s)	229, 1448
HhaI	GCGC	2 cut(s)	131, 162
Hin1I	GRCGYC	2 cut(s)	129, 192
Hin1II	CATG	7 cut(s)	217, 295, 424, 704, 766, 812, 1411
Hin6I	GCGC	2 cut(s)	129, 160
HinP1I	GCGC	2 cut(s)	129, 160
HincII	GTYRAC	1 cut(s)	1246
HindII	GTYRAC	1 cut(s)	1246
HindIII	AAGCTT	1 cut(s)	914
HinfI	GANTC	8 cut(s)	205, 269, 550, 674, 719, 798, 869, 1076
HpaII	CCGG	4 cut(s)	126, 306, 440, 1479
HphI	GGTGA	4 cut(s)	815, 1295, 1391, 1448
Hpy166II	GTNNAC	4 cut(s)	283, 838, 1246, 1464
Hpy188I	TCNGA	9 cut(s)	229, 469, 689, 724, 745, 958, 1066, 1081, 1448
Hpy188III	TCNNGA	5 cut(s)	196, 247, 334, 376, 1295
Hpy8I	GTNNAC	4 cut(s)	283, 838, 1246, 1464
Hpy99I	CGWCG	4 cut(s)	191, 194, 245, 1250
HpyAV	CCTTC	4 cut(s)	331, 474, 1037, 1366
HpyCH4III	ACNGT	2 cut(s)	954, 1243
HpyCH4IV	ACGT	3 cut(s)	192, 1287, 1466
HpyF10VI	GCNNNNNNNGC	2 cut(s)	137, 781
HpyF3I	CTNAG	4 cut(s)	47, 570, 723, 901
HpySE526I	ACGT	3 cut(s)	192, 1287, 1466
Hsp92I	GRCGYC	2 cut(s)	129, 192
Hsp92II	CATG	7 cut(s)	217, 295, 424, 704, 766, 812, 1411
HspAI	GCGC	2 cut(s)	129, 160
KasI	GGCGCC	1 cut(s)	128
KroI	GCCGGC	1 cut(s)	305
KroNI	GCCGGC	1 cut(s)	307
KspI	CCGCGG	1 cut(s)	44
Kzo9I	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
LmnI	GCTCC	4 cut(s)	171, 295, 1066, 1398
Lsp1109I	GCAGC	5 cut(s)	389, 1115, 1138, 1211, 1345
LweI	GCATC	2 cut(s)	463, 762
MaeI	CTAG	2 cut(s)	866, 1280
MaeII	ACGT	3 cut(s)	192, 1287, 1466
MaeIII	GTNAC	3 cut(s)	821, 1283, 1436
MalI	GATC	7 cut(s)	13, 251, 329, 432, 683, 759, 1458
MboI	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
MflI	RGATCY	2 cut(s)	681, 757
MhlI	GDGCHC	5 cut(s)	234, 260, 285, 300, 1064
MluCI	AATT	9 cut(s)	74, 89, 445, 1142, 1189, 1302, 1389, 1417, 1429
Mly113I	GGCGCC	1 cut(s)	129
MlyI	GAGTC	3 cut(s)	263, 559, 1085
MroNI	GCCGGC	1 cut(s)	305
MseI	TTAA	6 cut(s)	641, 918, 977, 1038, 1166, 1428
MslI	CAYNNNNRTG	1 cut(s)	1296
MspA1I	CMGCKG	3 cut(s)	43, 1106, 1122
MspCI	CTTAAG	2 cut(s)	640, 917
MspI	CCGG	4 cut(s)	126, 306, 440, 1479
MspR9I	CCNGG	3 cut(s)	176, 522, 829
Mva1269I	GAATGC	1 cut(s)	16
MvaI	CCWGG	3 cut(s)	176, 522, 829
MvnI	CGCG	2 cut(s)	43, 429
MwoI	GCNNNNNNNGC	2 cut(s)	137, 781
NaeI	GCCGGC	1 cut(s)	307
NarI	GGCGCC	1 cut(s)	129
NdeII	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
NgoMIV	GCCGGC	1 cut(s)	305
NlaIII	CATG	7 cut(s)	217, 295, 424, 704, 766, 812, 1411
NlaIV	GGNNCC	5 cut(s)	97, 130, 259, 651, 785
NmuCI	GTSAC	3 cut(s)	821, 1283, 1436
PcsI	WCGNNNNNNNCGW	3 cut(s)	270, 285, 504
PctI	GAATGC	1 cut(s)	16
PdiI	GCCGGC	1 cut(s)	307
PfeI	GAWTC	5 cut(s)	205, 674, 719, 798, 869
Pfl23II	CGTACG	1 cut(s)	30
PflFI	GACNNNGTC	2 cut(s)	191, 272
PkrI	GCNGC	9 cut(s)	42, 45, 311, 364, 404, 1105, 1153, 1201, 1360
PleI	GAGTC	3 cut(s)	263, 558, 1084
PluTI	GGCGCC	1 cut(s)	132
PpsI	GAGTC	3 cut(s)	263, 558, 1084
Psp124BI	GAGCTC	1 cut(s)	300
Psp6I	CCWGG	3 cut(s)	174, 520, 827
PspFI	CCCAGC	1 cut(s)	236
PspGI	CCWGG	3 cut(s)	174, 520, 827
PspLI	CGTACG	1 cut(s)	30
PspN4I	GGNNCC	5 cut(s)	97, 130, 259, 651, 785
PspPI	GGNCC	2 cut(s)	79, 593
PstNI	CAGNNNCTG	1 cut(s)	671
PsuI	RGATCY	2 cut(s)	681, 757
PsyI	GACNNNGTC	2 cut(s)	191, 272
PvuII	CAGCTG	1 cut(s)	1106
RsaI	GTAC	1 cut(s)	32
RsaNI	GTAC	1 cut(s)	31
RseI	CAYNNNNRTG	1 cut(s)	1296
SacI	GAGCTC	1 cut(s)	300
SacII	CCGCGG	1 cut(s)	44
SalI	GTCGAC	1 cut(s)	1244
SaqAI	TTAA	6 cut(s)	641, 918, 977, 1038, 1166, 1428
SatI	GCNGC	9 cut(s)	41, 44, 310, 363, 403, 1104, 1152, 1200, 1359
Sau3AI	GATC	7 cut(s)	11, 249, 327, 430, 681, 757, 1456
Sau96I	GGNCC	2 cut(s)	79, 593
SchI	GAGTC	3 cut(s)	263, 559, 1085
ScrFI	CCNGG	3 cut(s)	176, 522, 829
SduI	GDGCHC	5 cut(s)	234, 260, 285, 300, 1064
SfaNI	GCATC	2 cut(s)	463, 762
SfoI	GGCGCC	1 cut(s)	130
Sfr303I	CCGCGG	1 cut(s)	44
SgrAI	CRCCGGYG	1 cut(s)	125
SgrBI	CCGCGG	1 cut(s)	44
SinI	GGWCC	1 cut(s)	593
SmiMI	CAYNNNNRTG	1 cut(s)	1296
SmlI	CTYRAG	5 cut(s)	434, 640, 776, 890, 917
SmoI	CTYRAG	5 cut(s)	434, 640, 776, 890, 917
Sse9I	AATT	9 cut(s)	74, 89, 445, 1142, 1189, 1302, 1389, 1417, 1429
SspDI	GGCGCC	1 cut(s)	128
SspI	AATATT	2 cut(s)	530, 739
SspMI	CTAG	2 cut(s)	866, 1280
SstI	GAGCTC	1 cut(s)	300
StyD4I	CCNGG	3 cut(s)	174, 520, 827
StyI	CCWWGG	1 cut(s)	82
TaaI	ACNGT	2 cut(s)	954, 1243
TaiI	ACGT	3 cut(s)	195, 1290, 1469
TaqI	TCGA	3 cut(s)	456, 1245, 1341
TasI	AATT	9 cut(s)	74, 89, 445, 1142, 1189, 1302, 1389, 1417, 1429
TauI	GCSGC	4 cut(s)	43, 46, 312, 365
TfiI	GAWTC	5 cut(s)	205, 674, 719, 798, 869
Tru1I	TTAA	6 cut(s)	641, 918, 977, 1038, 1166, 1428
Tru9I	TTAA	6 cut(s)	641, 918, 977, 1038, 1166, 1428
TseFI	GTSAC	3 cut(s)	821, 1283, 1436
TseI	GCWGC	5 cut(s)	402, 1103, 1151, 1199, 1358
Tsp45I	GTSAC	3 cut(s)	821, 1283, 1436
TspDTI	ATGAA	6 cut(s)	17, 825, 923, 1280, 1314, 1335
TspGWI	ACGGA	2 cut(s)	262, 496
Tth111I	GACNNNGTC	2 cut(s)	191, 272
Vha464I	CTTAAG	2 cut(s)	640, 917
VneI	GTGCAC	1 cut(s)	281
VpaK11BI	GGWCC	1 cut(s)	593
XagI	CCTNNNNNAGG	1 cut(s)	173
XapI	RAATTY	3 cut(s)	445, 1142, 1189
XcmI	CCANNNNNNNNNTGG	1 cut(s)	388
XmiI	GTMKAC	3 cut(s)	837, 1245, 1463
XspI	CTAG	2 cut(s)	866, 1280
ZraI	GACGTC	1 cut(s)	193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.