AT1G44790

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013916
Physical Location & Seq
Reverse (-)
16912154 .. 16913664
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44790.1

Sequence Viewer

Length: 600 bp
ATGGCGATGTGGGTATTCGGGTACGGATCTTTAATATGGAAAACGGGTTTTCCTTTCGACGAAAGCCTCCCTGGTTTCATTAAAGGCTATCGCCGTGTCTTCCATCAAGGTAGTACTGATCATAGAGGAACTCCGGATTTTCCGGGAAGAACAGTGACATTAGAAGCTGCTCATGAAGAAGTTTGTTGTGGAGTTGCTTACAAGATAACTAAAGAGGAAGATAAAAGAGATGCTTTATTGCATTTGGAAGTGAGGGAGAAGCAATATGATCAGAAAGAGTATCTTGATTTCTTCACTGACTCTAATGCTTCGGAACCGGCTGTGGCAGGAGTAATGGTGTACATAGCTTCACCTGACAAGAAGAGCAACAACAACTACTTAGGACCAGCTCCTCTTGAAGATATTGCCAAACAGATAGTTAAAGCGAAAGGACCTTCAGGGCCAAACAGAGACTATCTCTTCAACCTCGAAGAGGCTCTTGCTCAACTCGGGTTTAAAGATAAACATGTCACAGATCTTGCAAACCAAGTTAGGCACATTCTTTCAGAATCCGAAGAGTTAGACATTGATGCCACCGCCGCCACCGCCAACAATGTCTAG

Protein Analysis

199

Amino Acids

22.23

Weight (kDa)

5.15

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 179 5e-54 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 3 cut(s) 576, 579, 585
AclWI GGATC 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 3 cut(s) 23, 115, 341
AfiI CCNNNNNNNGG 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 2 cut(s) 398, 463
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 3 cut(s) 167, 347, 389
AluI AGCT 3 cut(s) 167, 347, 389
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 1 cut(s) 167
AspS9I GGNCC 3 cut(s) 383, 431, 440
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 342
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 2 cut(s) 383, 431
BbsI GAAGAC 1 cut(s) 91
BbvI GCAGC 1 cut(s) 154
BccI CCATC 1 cut(s) 111
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 72
BclI TGATCA 2 cut(s) 118, 268
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 444
BfaI CTAG 1 cut(s) 598
BglII AGATCT 1 cut(s) 514
BisI GCNGC 2 cut(s) 168, 579
BlsI GCNGC 2 cut(s) 169, 580
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 72, 144
Bme18I GGWCC 2 cut(s) 383, 431
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 3 cut(s) 383, 431, 440
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 2 cut(s) 72, 144
BmsI GCATC 2 cut(s) 220, 559
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpuMI CCSGG 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 70
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse118I RCCGGY 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 472
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 154
BshFI GGCC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 134, 143, 317
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 444
BsnI GGCC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 488
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 4 cut(s) 26, 118, 268, 514
BspACI CCGC 3 cut(s) 576, 579, 585
BspANI GGCC 1 cut(s) 442
BspEI TCCGGA 1 cut(s) 133
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 315
BspPI GGATC 1 cut(s) 34
BspQI GCTCTTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 4 cut(s) 26, 118, 268, 514
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 4 cut(s) 356, 464, 465, 549
BstAUI TGTACA 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 470
BstKTI GATC 4 cut(s) 29, 121, 271, 517
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 4 cut(s) 26, 118, 268, 514
BstMWI GCNNNNNNNGC 2 cut(s) 578, 584
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 2 cut(s) 70, 142
BstV1I GCAGC 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 2 cut(s) 26, 514
BstYI RGATCY 2 cut(s) 26, 514
BsuRI GGCC 1 cut(s) 442
BtgZI GCGATG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 159, 294
CciI TCATGA 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 3 cut(s) 383, 431, 440
Csp6I GTAC 3 cut(s) 22, 114, 340
CviAII CATG 2 cut(s) 173, 506
CviJI RGCY 8 cut(s) 66, 87, 167, 320, 347, 389, 442, 476
CviKI_1 RGCY 8 cut(s) 66, 87, 167, 320, 347, 389, 442, 476
CviQI GTAC 3 cut(s) 22, 114, 340
DdeI CTNAG 1 cut(s) 379
DpnI GATC 4 cut(s) 28, 120, 270, 516
DpnII GATC 4 cut(s) 26, 118, 268, 514
DraI TTTAAA 1 cut(s) 496
Eam1104I CTCTTC 4 cut(s) 356, 464, 465, 549
EarI CTCTTC 4 cut(s) 356, 464, 465, 549
Eco47I GGWCC 2 cut(s) 383, 431
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 488
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 176, 509
FaiI YATR 6 cut(s) 37, 123, 174, 267, 344, 507
FalI AAGNNNNNCTT 6 cut(s) 217, 249, 267, 299, 462, 494
FatI CATG 2 cut(s) 172, 505
FbaI TGATCA 2 cut(s) 118, 268
Fnu4HI GCNGC 2 cut(s) 168, 579
Fsp4HI GCNGC 2 cut(s) 168, 579
FspBI CTAG 1 cut(s) 598
GluI GCNGC 2 cut(s) 168, 579
HaeIII GGCC 1 cut(s) 442
HapII CCGG 3 cut(s) 134, 143, 317
Hin1II CATG 2 cut(s) 176, 509
HinfI GANTC 2 cut(s) 299, 548
HpaII CCGG 3 cut(s) 134, 143, 317
HphI GGTGA 1 cut(s) 342
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 4 cut(s) 273, 313, 547, 553
Hpy188III TCNNGA 4 cut(s) 134, 173, 284, 395
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 444
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 241, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 584
HpyF3I CTNAG 1 cut(s) 379
Hsp92II CATG 2 cut(s) 176, 509
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 2 cut(s) 118, 268
Kzo9I GATC 4 cut(s) 26, 118, 268, 514
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 9 cut(s) 57, 84, 147, 156, 312, 330, 366, 399, 423
Lsp1109I GCAGC 1 cut(s) 154
LweI GCATC 2 cut(s) 220, 559
MaeI CTAG 1 cut(s) 598
MaeIII GTNAC 2 cut(s) 154, 508
MalI GATC 4 cut(s) 28, 120, 270, 516
MboI GATC 4 cut(s) 26, 118, 268, 514
MflI RGATCY 2 cut(s) 26, 514
MlyI GAGTC 1 cut(s) 293
MnlI CCTC 7 cut(s) 77, 119, 208, 246, 402, 466, 476
MroI TCCGGA 1 cut(s) 133
MseI TTAA 4 cut(s) 32, 81, 420, 495
MspI CCGG 3 cut(s) 134, 143, 317
MspR9I CCNGG 2 cut(s) 72, 144
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 578, 584
NciI CCSGG 1 cut(s) 144
NdeII GATC 4 cut(s) 26, 118, 268, 514
NlaIII CATG 2 cut(s) 176, 509
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 2 cut(s) 154, 508
NspI RCATGY 1 cut(s) 509
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 356
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 2 cut(s) 169, 580
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 431
PscI ACATGT 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 3 cut(s) 383, 431, 440
PspPPI RGGWCCY 1 cut(s) 431
PsuI RGATCY 2 cut(s) 26, 514
RsaI GTAC 3 cut(s) 23, 115, 341
RsaNI GTAC 3 cut(s) 22, 114, 340
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 4 cut(s) 32, 81, 420, 495
SatI GCNGC 2 cut(s) 168, 579
Sau3AI GATC 4 cut(s) 26, 118, 268, 514
Sau96I GGNCC 3 cut(s) 383, 431, 440
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 72, 144
SetI ASST 7 cut(s) 112, 169, 349, 355, 391, 436, 468
SfaNI GCATC 2 cut(s) 220, 559
SinI GGWCC 2 cut(s) 383, 431
SsiI CCGC 3 cut(s) 576, 579, 585
SspMI CTAG 1 cut(s) 598
StyD4I CCNGG 2 cut(s) 70, 142
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 2 cut(s) 57, 468
TatI WGTACW 2 cut(s) 113, 339
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 4 cut(s) 32, 81, 420, 495
Tru9I TTAA 4 cut(s) 32, 81, 420, 495
TscAI CASTG 2 cut(s) 159, 301
TseFI GTSAC 2 cut(s) 154, 508
TseI GCWGC 1 cut(s) 167
Tsp45I GTSAC 2 cut(s) 154, 508
TspDTI ATGAA 2 cut(s) 67, 189
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 2 cut(s) 159, 301
VpaK11BI GGWCC 2 cut(s) 383, 431
XagI CCTNNNNNAGG 1 cut(s) 470
XceI RCATGY 1 cut(s) 509
XspI CTAG 1 cut(s) 598
ZrmI AGTACT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.