FvH4_2g26710

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013916
Physical Location & Seq
Forward (+)
21407393 .. 21410061
2669 bp
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UTR
Exon/CDS
Intron
FvH4_2g26710.t1

Sequence Viewer

Length: 576 bp
ATGGTGATGTGGGTATTTGGTTATGGCTCTCTGATATGGAAGGCCGGGTTTAACTTCGATGAACGCGTCGTTGGTTTCATCAAAGACTATCGCCGTGTCTTCTACCAAGGCAGTACTGACCATAGAGGGACACCCGAGTTTCCAGGAAGAACAGTCACATTGGAACCTGCAGAGGGTGAAGTCTGTTGGGGAGCTGCCTACAAGATCAGCAAGAAGGAAGATCAGGAAATTGCTTTAGAGCACTTAGAAGTGAGGGAGAAGCAGTATGACAAGAAGGCTTATCTTGATTTCTTTACTGAGCCAACGGCTACGACTCCAGCTCTTTCAGGAGTAATGGTATACATTGCCTCTCCAGACAAAAAGCGTAATGTGAACTACTTGGGTCCTGCATCAATTGAAGATATTGCCAAACAGATTGTTGAGGCAAAAGGCCCCTCAGGACCTAATAGAGAGTACCTCTTTAAACTCGAAGAGGCCCTTCTTCAAATTGGATACAAAGACAAACATGTATTGGATCTTGCAAACGAAGTGAGGCGCATCATTTCAGAAAGGGATATGGAGCTCACTGCTTTATGA

Protein Analysis

192

Amino Acids

21.82

Weight (kDa)

5.53

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 179 1.2e-53 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 339
AccII CGCG 1 cut(s) 66
AclWI GGATC 1 cut(s) 522
AfaI GTAC 2 cut(s) 115, 455
AfiI CCNNNNNNNGG 1 cut(s) 173
AflIII ACRYGT 2 cut(s) 64, 505
AgsI TTSAA 2 cut(s) 398, 485
AjnI CCWGG 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 3 cut(s) 194, 320, 562
AluI AGCT 3 cut(s) 194, 320, 562
Alw21I GWGCWC 2 cut(s) 243, 564
AlwI GGATC 1 cut(s) 522
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 3 cut(s) 42, 430, 474
ApeKI GCWGC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 4 cut(s) 383, 431, 440, 475
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 2 cut(s) 16, 188
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 2 cut(s) 383, 440
AxyI CCTNAGG 1 cut(s) 436
BanII GRGCYC 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 243, 564
BbvI GCAGC 1 cut(s) 181
BceAI ACGGC 2 cut(s) 78, 321
BciT130I CCWGG 1 cut(s) 144
BciVI GTATCC 1 cut(s) 485
BcnI CCSGG 1 cut(s) 46
BfmI CTRYAG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 175
BfuI GTATCC 1 cut(s) 485
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 46, 144
Bme18I GGWCC 2 cut(s) 383, 440
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 4 cut(s) 383, 431, 440, 475
BmiI GGNNCC 3 cut(s) 165, 384, 433
BmrFI CCNGG 2 cut(s) 46, 144
BmsI GCATC 2 cut(s) 398, 546
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpmI CTGGAG 2 cut(s) 300, 336
BpuMI CCSGG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 106
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse21I CCTNAGG 1 cut(s) 436
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 288, 450
BseXI GCAGC 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 44, 432, 476
BsiHKAI GWGCWC 2 cut(s) 243, 564
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 3 cut(s) 44, 432, 476
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 243, 564
Bsp143I GATC 3 cut(s) 204, 220, 514
BspANI GGCC 3 cut(s) 44, 432, 476
BspCNI CTCAG 2 cut(s) 289, 449
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 165, 384, 433
BspMAI CTGCAG 1 cut(s) 172
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 1 cut(s) 522
BsrDI GCAATG 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 3 cut(s) 204, 220, 514
BssNAI GTATAC 1 cut(s) 340
BssT1I CCWWGG 1 cut(s) 106
Bst1107I GTATAC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 465
BstDEI CTNAG 3 cut(s) 244, 297, 436
BstFNI CGCG 1 cut(s) 66
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 3 cut(s) 207, 223, 517
BstMBI GATC 3 cut(s) 204, 220, 514
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 509
BstSCI CCNGG 2 cut(s) 44, 142
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BstZ17I GTATAC 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 436
BsuI GTATCC 1 cut(s) 485
BsuRI GGCC 3 cut(s) 44, 432, 476
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
BveI ACCTGC 1 cut(s) 175
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 4 cut(s) 383, 431, 440, 475
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 114, 454
CviAII CATG 1 cut(s) 506
CviQI GTAC 2 cut(s) 114, 454
DdeI CTNAG 3 cut(s) 244, 297, 436
DpnI GATC 3 cut(s) 206, 222, 516
DpnII GATC 3 cut(s) 204, 220, 514
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 2 cut(s) 383, 440
Eco53kI GAGCTC 1 cut(s) 562
Eco81I CCTNAGG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 562
EcoO109I RGGNCCY 4 cut(s) 383, 431, 440, 475
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 1 cut(s) 509
FaiI YATR 8 cut(s) 24, 37, 123, 267, 340, 507, 557, 574
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 1 cut(s) 142
FatI CATG 1 cut(s) 505
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 195
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 195
GlaI GCGC 1 cut(s) 536
GluI GCNGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 300, 336
HaeIII GGCC 3 cut(s) 44, 432, 476
HapII CCGG 1 cut(s) 45
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 537
Hin1II CATG 1 cut(s) 509
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 1 cut(s) 313
HpaII CCGG 1 cut(s) 45
HphI GGTGA 2 cut(s) 16, 188
Hpy166II GTNNAC 2 cut(s) 340, 373
Hpy188I TCNGA 2 cut(s) 33, 547
Hpy188III TCNNGA 5 cut(s) 224, 284, 327, 353, 438
Hpy8I GTNNAC 2 cut(s) 340, 373
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 34, 208, 268, 488
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 3 cut(s) 170, 389, 521
HpyF3I CTNAG 3 cut(s) 244, 297, 436
Hsp92II CATG 1 cut(s) 509
HspAI GCGC 1 cut(s) 535
Kzo9I GATC 3 cut(s) 204, 220, 514
LmnI GCTCC 2 cut(s) 191, 559
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 398, 546
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 206, 222, 516
MboI GATC 3 cut(s) 204, 220, 514
MboII GAAGA 6 cut(s) 91, 159, 230, 410, 473, 482
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 243, 564
MluCI AATT 3 cut(s) 228, 393, 486
MluI ACGCGT 1 cut(s) 64
MlyI GAGTC 1 cut(s) 307
MnlI CCTC 9 cut(s) 119, 166, 246, 358, 415, 445, 466, 467, 525
MseI TTAA 2 cut(s) 51, 462
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 46, 144
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 66
NciI CCSGG 1 cut(s) 46
NdeII GATC 3 cut(s) 204, 220, 514
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 3 cut(s) 165, 384, 433
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 509
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 2 cut(s) 383, 440
PscI ACATGT 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 564
Psp5II RGGWCCY 2 cut(s) 383, 440
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 3 cut(s) 165, 384, 433
PspPI GGNCC 4 cut(s) 383, 431, 440, 475
PspPPI RGGWCCY 2 cut(s) 383, 440
PstI CTGCAG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 2 cut(s) 115, 455
RsaNI GTAC 2 cut(s) 114, 454
SacI GAGCTC 1 cut(s) 564
SaqAI TTAA 2 cut(s) 51, 462
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 3 cut(s) 204, 220, 514
Sau96I GGNCC 4 cut(s) 383, 431, 440, 475
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 46, 144
SduI GDGCHC 2 cut(s) 243, 564
SetI ASST 6 cut(s) 169, 196, 322, 445, 459, 564
SfaNI GCATC 2 cut(s) 398, 546
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 2 cut(s) 383, 440
Sse9I AATT 3 cut(s) 228, 393, 486
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 44, 142
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 2 cut(s) 57, 468
TasI AATT 3 cut(s) 228, 393, 486
TatI WGTACW 1 cut(s) 113
Tru1I TTAA 2 cut(s) 51, 462
Tru9I TTAA 2 cut(s) 51, 462
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 67, 75
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 2 cut(s) 383, 440
XceI RCATGY 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 339
ZrmI AGTACT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.