Rh6CG379900

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013916
Physical Location & Seq
Forward (+)
56642459 .. 56645340
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG379900.1

Sequence Viewer

Length: 510 bp
ATGGTCATGTGGGTATTTGGATATGGTTCTTTGATATGGAAAGCCGGCTTTAACTTCGATGAACGCATCGTTGGATTTATCAAAGACTATCGCCGTGTCTTCTACCAAGGCAGTACTGACCATAGAGGGACTCCTGAGTTTCCAGGAAGAACAGTCACATTGGAACCTGCAGAGGGGGAAGTCTGTTGGGGAGCTGCCTACAAGATCTCAAAGAAGGAAGATCAAGAAATTGCTTTAGAGCACTTAGAAGTGAGGGAGAAGCAGTATGACAAGAAGGCTTATCTTCATTTCTTTACTGAGCCGACGGGTACGACTCCAGCTCTTTCAGGAGTAATGGTATATATTGCCTCTCCAGATAAAAAGCTTAATGTGAACTATTTGGGTCCTGCATCCATTGAAGATATTGCCAAGTATGTAAATTTCATCTACCAAGCTGTTATTTGGTTAAATATTAATCCAAGTATTCAAAACCTAAATGAAGTAAGGAGCATTTTGCTTCTCCAAATCTAG

Protein Analysis

169

Amino Acids

19.39

Weight (kDa)

5.94

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 139 1.4e-38 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 175
AcsI RAATTY 1 cut(s) 418
AfaI GTAC 2 cut(s) 115, 310
AfiI CCNNNNNNNGG 1 cut(s) 173
AgsI TTSAA 2 cut(s) 398, 467
AjnI CCWGG 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 4 cut(s) 194, 320, 364, 434
AluI AGCT 4 cut(s) 194, 320, 364, 434
Alw21I GWGCWC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 418
AseI ATTAAT 1 cut(s) 453
AspS9I GGNCC 1 cut(s) 383
AvaII GGWCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 144
BfaI CTAG 1 cut(s) 508
BfmI CTRYAG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 175
BglII AGATCT 1 cut(s) 204
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 2 cut(s) 165, 384
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 2 cut(s) 75, 398
BpiI GAAGAC 1 cut(s) 91
BpmI CTGGAG 2 cut(s) 300, 336
BsaJI CCNNGG 1 cut(s) 106
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse118I RCCGGY 1 cut(s) 44
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMII CTCAG 2 cut(s) 126, 288
BseXI GCAGC 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 204, 220
BspCNI CTCAG 2 cut(s) 127, 289
BspLI GGNNCC 2 cut(s) 165, 384
BspMAI CTGCAG 1 cut(s) 172
BspMI ACCTGC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 2 cut(s) 204, 220
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 3 cut(s) 135, 244, 297
BstF5I GGATG 1 cut(s) 389
BstKTI GATC 2 cut(s) 207, 223
BstMBI GATC 2 cut(s) 204, 220
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BtsCI GGATG 1 cut(s) 389
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 114, 309
CviAII CATG 1 cut(s) 7
CviJI RGCY 8 cut(s) 44, 48, 194, 278, 301, 320, 364, 434
CviKI_1 RGCY 8 cut(s) 44, 48, 194, 278, 301, 320, 364, 434
CviQI GTAC 2 cut(s) 114, 309
DdeI CTNAG 3 cut(s) 135, 244, 297
DpnI GATC 2 cut(s) 206, 222
DpnII GATC 2 cut(s) 204, 220
Eco130I CCWWGG 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 383
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 1 cut(s) 10
FaiI YATR 8 cut(s) 8, 24, 37, 123, 267, 340, 342, 414
FaqI GGGAC 1 cut(s) 142
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 1 cut(s) 508
GluI GCNGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 300, 336
HapII CCGG 1 cut(s) 45
Hin1II CATG 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 2 cut(s) 130, 313
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 373
Hpy188III TCNNGA 4 cut(s) 134, 224, 327, 353
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 208, 268
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 170, 389
HpyF3I CTNAG 3 cut(s) 135, 244, 297
Hsp92II CATG 1 cut(s) 10
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 204, 220
LmnI GCTCC 2 cut(s) 191, 486
LpnPI CCDG 9 cut(s) 58, 129, 147, 156, 180, 312, 330, 366, 399
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 75, 398
MaeI CTAG 1 cut(s) 508
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 206, 222
MboI GATC 2 cut(s) 204, 220
MboII GAAGA 5 cut(s) 91, 159, 230, 275, 410
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 2 cut(s) 228, 418
MlyI GAGTC 2 cut(s) 124, 307
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 4 cut(s) 119, 166, 246, 358
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 4 cut(s) 51, 366, 446, 453
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
NaeI GCCGGC 1 cut(s) 46
NdeII GATC 2 cut(s) 204, 220
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 1 cut(s) 10
NlaIV GGNNCC 2 cut(s) 165, 384
NmuCI GTSAC 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 2 cut(s) 124, 307
PpsI GAGTC 2 cut(s) 124, 307
PpuMI RGGWCCY 1 cut(s) 383
PshBI ATTAAT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 165, 384
PspPI GGNCC 1 cut(s) 383
PspPPI RGGWCCY 1 cut(s) 383
PstI CTGCAG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 2 cut(s) 115, 310
RsaNI GTAC 2 cut(s) 114, 309
SaqAI TTAA 4 cut(s) 51, 366, 446, 453
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 2 cut(s) 204, 220
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 2 cut(s) 124, 307
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 243
SetI ASST 6 cut(s) 169, 196, 322, 366, 436, 474
SfaNI GCATC 2 cut(s) 75, 398
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 1 cut(s) 383
Sse9I AATT 2 cut(s) 228, 418
SspI AATATT 1 cut(s) 451
SspMI CTAG 1 cut(s) 508
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 57
TasI AATT 2 cut(s) 228, 418
TatI WGTACW 1 cut(s) 113
Tru1I TTAA 4 cut(s) 51, 366, 446, 453
Tru9I TTAA 4 cut(s) 51, 366, 446, 453
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 4 cut(s) 75, 275, 412, 492
VpaK11BI GGWCC 1 cut(s) 383
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 1 cut(s) 418
XspI CTAG 1 cut(s) 508
ZrmI AGTACT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.