Rh6AG365400

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0013916
Physical Location & Seq
Forward (+)
56429774 .. 56432327
2554 bp
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UTR
Exon/CDS
Intron
Rh6AG365400.1

Sequence Viewer

Length: 564 bp
ATGGTCATGTGGGTATTTGGTTATGGTTCTTTGATATGGAAAGCCGGCTTTAACTTCGATGAACGCGTCGTCGGATTCATCAAAGACTATCGCCGTGTCTTCTACCAAGGCAGTACTGACCACAGAGGGACTCCTGAGTTTCCAGGAAGAACAGTCACATTGGAACCTGCAGAGGGGGAAGTCTGTTGGGGAGCTGCCTACAAGATCTCAAAGAAGGAAGATCAAGAAATTGCTTTAGAGCACTTAGAAGTGAGGGAGAAGCAGTATGACAAGAAGGCTTATCTTCATTTCTTTACTGAGCCGACAGCTACGACTCCAGCTCTTTCAGGAGTAATGGTATATATTGCCTCTCCAGATAAAAAGCTTAATGTGAACTACTTGGGTCCTGCATCCATTGAAGATATTGCCAAACAGATTGTTCAGGCAAAAGGTCCCTCAGGACCTAATAGAGAGTATCTTTTTAAACTTGAAGAGGCCCTTCTTCAATTTGGTAATGTTAATTTGGTGCCCCATGAATTTATCTTAGTTATGTATGCTACATTAGAACAACTTCTTTTCCTTTGA

Protein Analysis

187

Amino Acids

21.32

Weight (kDa)

5.62

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 164 1.4e-50 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 175
AccB1I GGYRCC 1 cut(s) 505
AccII CGCG 1 cut(s) 66
AcsI RAATTY 1 cut(s) 515
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 3 cut(s) 398, 470, 485
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 4 cut(s) 194, 308, 320, 364
AluI AGCT 4 cut(s) 194, 308, 320, 364
Alw21I GWGCWC 1 cut(s) 243
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 515
Asp700I GAANNNNTTC 1 cut(s) 549
AspS9I GGNCC 4 cut(s) 383, 431, 440, 475
AvaII GGWCC 3 cut(s) 383, 431, 440
AxyI CCTNAGG 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 175
BglII AGATCT 1 cut(s) 204
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 3 cut(s) 383, 431, 440
BmgT120I GGNCC 4 cut(s) 383, 431, 440, 475
BmiI GGNNCC 4 cut(s) 165, 384, 433, 507
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 398
BpiI GAAGAC 1 cut(s) 91
BpmI CTGGAG 2 cut(s) 300, 336
BsaJI CCNNGG 1 cut(s) 106
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse118I RCCGGY 1 cut(s) 44
Bse21I CCTNAGG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMII CTCAG 3 cut(s) 126, 288, 450
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 476
BshNI GGYRCC 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 2 cut(s) 142, 417
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 142, 417
BsnI GGCC 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 243, 510
Bsp143I GATC 2 cut(s) 204, 220
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 3 cut(s) 127, 289, 449
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 165, 384, 433, 507
BspMAI CTGCAG 1 cut(s) 172
BspMI ACCTGC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 2 cut(s) 204, 220
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 5 cut(s) 135, 244, 297, 436, 523
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 2 cut(s) 207, 223
BstMBI GATC 2 cut(s) 204, 220
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 436
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 389
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 4 cut(s) 383, 431, 440, 475
CseI GACGC 1 cut(s) 55
Csp6I GTAC 1 cut(s) 114
CviAII CATG 2 cut(s) 7, 512
CviJI RGCY 9 cut(s) 44, 48, 194, 278, 301, 308, 320, 364, 476
CviKI_1 RGCY 9 cut(s) 44, 48, 194, 278, 301, 308, 320, 364, 476
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 5 cut(s) 135, 244, 297, 436, 523
DpnI GATC 2 cut(s) 206, 222
DpnII GATC 2 cut(s) 204, 220
DraI TTTAAA 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 106
Eco47I GGWCC 3 cut(s) 383, 431, 440
Eco81I CCTNAGG 1 cut(s) 436
EcoO109I RGGNCCY 4 cut(s) 383, 431, 440, 475
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 10, 515
FaiI YATR 9 cut(s) 8, 24, 37, 267, 340, 342, 513, 530, 534
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 2 cut(s) 142, 417
FatI CATG 2 cut(s) 6, 511
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 1 cut(s) 195
GluI GCNGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 300, 336
HaeIII GGCC 1 cut(s) 476
HapII CCGG 1 cut(s) 45
HgaI GACGC 1 cut(s) 55
Hin1II CATG 2 cut(s) 10, 515
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 3 cut(s) 75, 130, 313
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 5 cut(s) 134, 224, 327, 353, 438
Hpy8I GTNNAC 1 cut(s) 373
Hpy99I CGWCG 2 cut(s) 71, 74
HpyAV CCTTC 3 cut(s) 208, 268, 488
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 170, 389
HpyF3I CTNAG 5 cut(s) 135, 244, 297, 436, 523
Hsp92II CATG 2 cut(s) 10, 515
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 204, 220
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 206, 222
MboI GATC 2 cut(s) 204, 220
MboII GAAGA 7 cut(s) 91, 159, 230, 275, 410, 473, 482
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 243, 510
MluCI AATT 4 cut(s) 228, 485, 499, 515
MluI ACGCGT 1 cut(s) 64
MlyI GAGTC 2 cut(s) 124, 307
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 6 cut(s) 119, 166, 246, 358, 445, 466
MroNI GCCGGC 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 4 cut(s) 51, 366, 462, 498
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 66
NaeI GCCGGC 1 cut(s) 46
NdeII GATC 2 cut(s) 204, 220
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 2 cut(s) 10, 515
NlaIV GGNNCC 4 cut(s) 165, 384, 433, 507
NmuCI GTSAC 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PdiI GCCGGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 2 cut(s) 124, 307
PpsI GAGTC 2 cut(s) 124, 307
PpuMI RGGWCCY 3 cut(s) 383, 431, 440
Psp5II RGGWCCY 3 cut(s) 383, 431, 440
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 4 cut(s) 165, 384, 433, 507
PspPI GGNCC 4 cut(s) 383, 431, 440, 475
PspPPI RGGWCCY 3 cut(s) 383, 431, 440
PstI CTGCAG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SaqAI TTAA 4 cut(s) 51, 366, 462, 498
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 2 cut(s) 204, 220
Sau96I GGNCC 4 cut(s) 383, 431, 440, 475
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 2 cut(s) 124, 307
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 2 cut(s) 243, 510
SetI ASST 7 cut(s) 169, 196, 310, 322, 366, 433, 445
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 3 cut(s) 383, 431, 440
Sse9I AATT 4 cut(s) 228, 485, 499, 515
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 57
TasI AATT 4 cut(s) 228, 485, 499, 515
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 4 cut(s) 51, 366, 462, 498
Tru9I TTAA 4 cut(s) 51, 366, 462, 498
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 4 cut(s) 67, 75, 275, 528
VpaK11BI GGWCC 3 cut(s) 383, 431, 440
XapI RAATTY 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 549
ZrmI AGTACT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.