Rh6DG366500

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013916
Physical Location & Seq
Forward (+)
56308267 .. 56310872
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG366500.1

Sequence Viewer

Length: 687 bp
ATGTGCATCATTGAACCAAGTCACCAAACACAGTGTTCGGGCCCCTCTCCTTCGTCATCGCCCCTCAGCTTTCCGACAGAAACTCATTGCCGAAAAAGCTGTAAGATTCTGATCGGGGAAAAAATGGTCATGTGGGTATTTGGTTATGGTTCTTTGATATGGAAAGCCGGCTTTAACTTCGATGAACGCGTCGTTGGTTTCATCAAAGACTATCGCCGTGTCTTCTACCAAGGCAGTACTGACCATAGAGGGACTCCTGAGTTTCCAGGAAGAACAGTCACATTGGAACCTGCAGAGGGGGAAGTCTGTTGGGGAGCTGCCTACAAGATCTCAAAGAAGGAAGATCAAGAAATTGCTTTAGAGCACTTAGAAGTGAGGGAGAAGCAGTATGACAAGAAGGCTTATCTTGATTTCTTTACTGAGCCGACGGCTACGACTCCAGCTCTTTCAGGAGTAATGGTATACATTGCCTCTCCAGATAAAAAGCTTAATGTGAACTATTTGGGTCCTGCATCCATTGAAGATATTGCCAAACAGATTGTTCAGGCGAAAGGTCCCTCAGGACCTAATAGAGAGTATCTTTTTAAACTTGAAGAGGCCCTTCGTCAATTTGGTAATGTTAATTTGGTGCCCCATGAATTTATCTTAGTTGTGTATGCTACATTAGAACAACTTCTTTTCCTTTGA

Protein Analysis

228

Amino Acids

25.74

Weight (kDa)

6.0

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 44 - 205 8.7e-51 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013916)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G44790
fragaria_vesca FvH4_2g26710
malus_domestica MD00G1157300.v1.1
prunus_persica Prupe.1G500300_v2.0.a1
pyrus_communis pycom12654g00130
rosa_chinensis RchiOBHm_Chr6g0295581
rosa_laevigata RLG00000011787
rosa_multiflora Rmu_sc0005000.1_g000008
rosa_roxburghii Rroxscaffold_7G00172350
rosa_rugosa Rorug06G0252200 Rorug06G0252300 Rorug06G0252400
rosa_samantha Rh6AG365400 Rh6BG373600 Rh6CG379900 Rh6DG366500
rosa_wichuraiana Rw6G031900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 628
AccI GTMKAC 1 cut(s) 462
AccII CGCG 1 cut(s) 189
AcsI RAATTY 1 cut(s) 638
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 3 cut(s) 14, 521, 593
AjnI CCWGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
AluBI AGCT 5 cut(s) 69, 99, 317, 443, 487
AluI AGCT 5 cut(s) 69, 99, 317, 443, 487
Alw21I GWGCWC 1 cut(s) 366
AoxI GGCC 2 cut(s) 40, 597
ApaI GGGCCC 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 672
AspS9I GGNCC 6 cut(s) 40, 41, 506, 554, 563, 598
AsuHPI GGTGA 1 cut(s) 14
AvaII GGWCC 3 cut(s) 506, 554, 563
AxyI CCTNAGG 1 cut(s) 559
BaeGI GKGCMC 2 cut(s) 44, 633
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 304
BceAI ACGGC 2 cut(s) 201, 444
BciT130I CCWGG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 298
BglII AGATCT 1 cut(s) 327
BisI GCNGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 319
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 3 cut(s) 506, 554, 563
BmgT120I GGNCC 6 cut(s) 40, 41, 506, 554, 563, 598
BmiI GGNNCC 6 cut(s) 42, 43, 288, 507, 556, 630
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 2 cut(s) 15, 521
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 2 cut(s) 423, 459
Bpu10I CCTNAGC 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 229
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 444, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse118I RCCGGY 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 559
Bse3DI GCAATG 2 cut(s) 85, 465
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 1 cut(s) 512
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMI GCAATG 2 cut(s) 85, 465
BseMII CTCAG 4 cut(s) 79, 249, 411, 573
BseSI GKGCMC 2 cut(s) 44, 633
BseXI GCAGC 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 42, 599
BshNI GGYRCC 1 cut(s) 628
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 2 cut(s) 265, 540
BslI CCNNNNNNNGG 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 265, 540
BsnI GGCC 2 cut(s) 42, 599
Bsp120I GGGCCC 1 cut(s) 40
Bsp1286I GDGCHC 3 cut(s) 44, 366, 633
Bsp143I GATC 3 cut(s) 111, 327, 343
BspANI GGCC 2 cut(s) 42, 599
BspCNI CTCAG 4 cut(s) 78, 250, 412, 572
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 6 cut(s) 42, 43, 288, 507, 556, 630
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 628
BsrDI GCAATG 2 cut(s) 85, 465
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 3 cut(s) 111, 327, 343
BssNAI GTATAC 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 229
Bst1107I GTATAC 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 33, 277
Bst6I CTCTTC 1 cut(s) 588
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 6 cut(s) 65, 258, 367, 420, 559, 646
BstF5I GGATG 1 cut(s) 512
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 3 cut(s) 114, 330, 346
BstMBI GATC 3 cut(s) 111, 327, 343
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 291
BstSLI GKGCMC 2 cut(s) 44, 633
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BstZ17I GTATAC 1 cut(s) 463
Bsu36I CCTNAGG 1 cut(s) 559
BsuRI GGCC 2 cut(s) 42, 599
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 512
BtsIMutI CAGTG 1 cut(s) 38
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 6 cut(s) 40, 41, 506, 554, 563, 598
CseI GACGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 237
CviAII CATG 2 cut(s) 130, 635
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 6 cut(s) 65, 258, 367, 420, 559, 646
DpnI GATC 3 cut(s) 113, 329, 345
DpnII GATC 3 cut(s) 111, 327, 343
DraI TTTAAA 1 cut(s) 586
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 3 cut(s) 506, 554, 563
Eco81I CCTNAGG 1 cut(s) 559
EcoO109I RGGNCCY 5 cut(s) 41, 506, 554, 563, 598
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 2 cut(s) 133, 638
FaiI YATR 8 cut(s) 131, 147, 160, 246, 390, 463, 636, 657
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 265, 540
FatI CATG 2 cut(s) 129, 634
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 1 cut(s) 318
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 318
GluI GCNGC 1 cut(s) 318
GsuI CTGGAG 2 cut(s) 423, 459
HaeIII GGCC 2 cut(s) 42, 599
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 178
Hin1II CATG 2 cut(s) 133, 638
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 106, 253, 436
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 463, 496
Hpy188I TCNGA 2 cut(s) 75, 111
Hpy188III TCNNGA 6 cut(s) 257, 347, 407, 450, 476, 561
Hpy8I GTNNAC 2 cut(s) 463, 496
Hpy99I CGWCG 2 cut(s) 194, 430
HpyAV CCTTC 4 cut(s) 60, 331, 391, 611
HpyCH4III ACNGT 2 cut(s) 33, 277
HpyCH4V TGCA 3 cut(s) 6, 293, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 6 cut(s) 65, 258, 367, 420, 559, 646
Hsp92II CATG 2 cut(s) 133, 638
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 111, 327, 343
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 2 cut(s) 15, 521
MaeIII GTNAC 2 cut(s) 20, 277
MalI GATC 3 cut(s) 113, 329, 345
MboI GATC 3 cut(s) 111, 327, 343
MboII GAAGA 5 cut(s) 214, 282, 353, 533, 605
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 3 cut(s) 44, 366, 633
MluCI AATT 4 cut(s) 351, 608, 622, 638
MluI ACGCGT 1 cut(s) 187
MlyI GAGTC 2 cut(s) 247, 430
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 8 cut(s) 55, 74, 242, 289, 369, 481, 568, 589
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 672
MseI TTAA 4 cut(s) 174, 489, 585, 621
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 96
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 3 cut(s) 111, 327, 343
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 2 cut(s) 133, 638
NlaIV GGNNCC 6 cut(s) 42, 43, 288, 507, 556, 630
NmuCI GTSAC 2 cut(s) 20, 277
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 672
PfeI GAWTC 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 319
PleI GAGTC 2 cut(s) 247, 430
PpsI GAGTC 2 cut(s) 247, 430
PpuMI RGGWCCY 3 cut(s) 506, 554, 563
Psp5II RGGWCCY 3 cut(s) 506, 554, 563
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 6 cut(s) 42, 43, 288, 507, 556, 630
PspOMI GGGCCC 1 cut(s) 40
PspPI GGNCC 6 cut(s) 40, 41, 506, 554, 563, 598
PspPPI RGGWCCY 3 cut(s) 506, 554, 563
PstI CTGCAG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
SaqAI TTAA 4 cut(s) 174, 489, 585, 621
SatI GCNGC 1 cut(s) 318
Sau3AI GATC 3 cut(s) 111, 327, 343
Sau96I GGNCC 6 cut(s) 40, 41, 506, 554, 563, 598
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 2 cut(s) 247, 430
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 3 cut(s) 44, 366, 633
SetI ASST 8 cut(s) 71, 101, 292, 319, 445, 489, 556, 568
SfaNI GCATC 2 cut(s) 15, 521
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 3 cut(s) 506, 554, 563
Sse9I AATT 4 cut(s) 351, 608, 622, 638
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 2 cut(s) 33, 277
TaqI TCGA 1 cut(s) 180
TasI AATT 4 cut(s) 351, 608, 622, 638
TatI WGTACW 1 cut(s) 236
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 4 cut(s) 174, 489, 585, 621
Tru9I TTAA 4 cut(s) 174, 489, 585, 621
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 2 cut(s) 20, 277
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 2 cut(s) 20, 277
TspDTI ATGAA 3 cut(s) 190, 198, 651
TspRI CASTG 1 cut(s) 38
VpaK11BI GGWCC 3 cut(s) 506, 554, 563
XapI RAATTY 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.