AT1G48900

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009104
Physical Location & Seq
Reverse (-)
18084715 .. 18087934
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G48900.2

Sequence Viewer

Length: 1488 bp
ATGGTGTTGGCGGAGCTAGGCGGGCGGATTACCCGCGCGATACAGCAGATGAGCAATGTGACAATTATCGATGAGAAGGCTTTGAATGAATGCTTGAACGAGATCACTCGTGCTTTGCTTCAATCCGATGTTTCATTTCCCCTTGTGAAAGAGATGCAGAGTAATATTAAGAAAATCGTCAATCTTGAAGATCTAGCAGCTGGTCACAACAAGCGCCGGATCATTGAACAGGCTATCTTTAGTGAACTCTGTAAGATGTTGGATCCAGGGAAGCCTGCGTTTGCTCCTAAAAAAGCTAAAGCTAGTGTAGTTATGTTTGTTGGATTACAAGGTGCTGGAAAGACGACAACTTGTACTAAGTATGCTTATTATCATCAGAAGAAAGGATACAAACCAGCTTTGGTTTGTGCGGATACTTTCAGGGCTGGTGCGTTCGATCAGCTGAAACAGAATGCTACCAAGGCTAAGATCCCCTTTTATGGAAGCTACACGGAATCCGATCCTGTGAAAATTGCTGTTGAGGGAGTAGATACGTTTAAGAAAGAAAACTGTGATCTCATTATTGTTGATACCAGTGGTCGCCATAAACAAGAAGCTTCTCTCTTTGAAGAAATGCGTCAAGTTGCTGAAGCAACGAAACCAGATCTTGTTATATTTGTTATGGATAGCAGTATTGGTCAAGCTGCATTTGATCAAGCTCAAGCCTTTAAGCAGAGTGTTGCTGTTGGAGCTGTAATTATTACTAAGATGGACGGCCATGCCAAGGGTGGTGGGGCTCTTAGCGCTGTTGCAGCTACGAAAAGTCCTGTGATATTTATCGGAACAGGAGAGCATATGGATGAGTTTGAAGTGTTTGACGTCAAACCATTTGTCAGCCGTCTCTTAGGAATGGGTGATTGGTCTGGATTCGTGGATAAGCTACAAGAGGTAGTACCTAAAGATCAACAGCCTGAACTTCTGGAAAAGCTCTCTCAGGGTAACTTTACGTTGAGAATTATGTACGACCAGTTCCAGAACATACTGAACATGGGTCCACTTAAGGAGGTTTTCTCAATGCTGCCTGGAATTAGTGCTGAAATGATGCCGAAAGGACATGAGAAAGAAAGCCAGGCGAAGATAAAGCGGTACATGACAATGATGGATTCTATGACAAATGATGAACTGGACAGCTCAAACCCAAAGGTGTTTAACGAGTCACGGATGATGCGGATAGCAAGAGGGTCAGGGAGGCAAGTAAGAGAAGTGATGGAGATGTTGGAAGAGTACAAGAGGCTAGCGAAGATATGGAGCAAGATGAAAGGACTCAAGATCCCAAAGAATGGAGATATGAGTGCACTCTCGAGAAACATGAACGCACAGCACATGAGCAAGGTCCTCCCACCTCAGATGCTCAAGCAGATTGGCGGCATGGGCGGTCTGCAGAGTCTAATGAAGCAGATGGGTTCCGGCAAGGACATGATGGGAATGTTCGGTGGCGGAGACAAGTAG

Protein Analysis

495

Amino Acids

54.78

Weight (kDa)

9.02

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 6 - 83 1.5e-13 SRP54-type protein, helical bundle domain
SRP54 PF00448 102 - 296 2.7e-78 SRP54-type protein, GTPase domain
cobW PF02492 103 - 253 3.9e-06 CobW/HypB/UreG, nucleotide-binding domain
SRP_SPB PF02978 327 - 419 1.5e-25 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 861
AccB7I CCANNNNNTGG 1 cut(s) 1319
AccII CGCG 2 cut(s) 36, 38
AclWI GGATC 6 cut(s) 227, 257, 270, 463, 494, 1303
AcoI YGGCCR 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 5 cut(s) 355, 933, 1001, 1127, 1265
AfeI AGCGCT 1 cut(s) 784
AfiI CCNNNNNNNGG 3 cut(s) 479, 763, 1319
AflII CTTAAG 1 cut(s) 1037
AgsI TTSAA 7 cut(s) 85, 97, 122, 188, 227, 608, 848
AjnI CCWGG 3 cut(s) 265, 1060, 1107
AjuI GAANNNNNNNTTGG 2 cut(s) 880, 912
Alw21I GWGCWC 1 cut(s) 1336
Alw26I GTCTC 2 cut(s) 884, 1473
Alw44I GTGCAC 1 cut(s) 1332
AlwI GGATC 6 cut(s) 227, 257, 270, 463, 494, 1303
Ama87I CYCGRG 1 cut(s) 1339
Aor51HI AGCGCT 1 cut(s) 784
AoxI GGCC 1 cut(s) 754
ApaLI GTGCAC 1 cut(s) 1332
ApeKI GCWGC 4 cut(s) 197, 683, 791, 1057
ArsI GACNNNNNNTTYG 2 cut(s) 855, 887
AspLEI GCGC 3 cut(s) 38, 216, 785
AspS9I GGNCC 2 cut(s) 1031, 1372
AsuHPI GGTGA 1 cut(s) 905
AsuNHI GCTAGC 1 cut(s) 1273
AvaI CYCGRG 1 cut(s) 1339
AvaII GGWCC 2 cut(s) 1031, 1372
BaeGI GKGCMC 1 cut(s) 1336
BamHI GGATCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 778
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BauI CACGAG 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 1336
BbvI GCAGC 4 cut(s) 209, 670, 803, 1044
BccI CCATC 5 cut(s) 742, 1132, 1240, 1432, 1453
BceAI ACGGC 2 cut(s) 769, 861
BciT130I CCWGG 3 cut(s) 267, 1062, 1109
BciVI GTATCC 2 cut(s) 380, 406
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 884, 1473
BfaI CTAG 4 cut(s) 17, 194, 303, 1274
BfmI CTRYAG 1 cut(s) 1418
BfoI RGCGCY 2 cut(s) 217, 786
BfrI CTTAAG 1 cut(s) 1037
BfuI GTATCC 2 cut(s) 380, 406
BglII AGATCT 2 cut(s) 190, 643
BisI GCNGC 5 cut(s) 198, 684, 792, 1058, 1405
BlsI GCNGC 5 cut(s) 199, 685, 793, 1059, 1406
Bme1390I CCNGG 3 cut(s) 267, 1062, 1109
Bme18I GGWCC 2 cut(s) 1031, 1372
BmeT110I CYCGRG 1 cut(s) 1339
BmgT120I GGNCC 2 cut(s) 1031, 1372
BmiI GGNNCC 3 cut(s) 264, 1032, 1444
BmrFI CCNGG 3 cut(s) 267, 1062, 1109
BmsI GCATC 4 cut(s) 144, 1071, 1194, 1377
BmtI GCTAGC 1 cut(s) 1277
BplI GAGNNNNNCTC 2 cut(s) 1034, 1066
BpuEI CTTGAG 3 cut(s) 684, 1289, 1376
Bsa29I ATCGAT 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 858
BsaJI CCNNGG 3 cut(s) 266, 459, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 479, 763, 1319
Bse1I ACTGG 3 cut(s) 573, 1006, 1167
Bse3DI GCAATG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 815
BseBI CCWGG 3 cut(s) 267, 1062, 1109
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 266, 459, 762
BseGI GGATG 2 cut(s) 844, 1206
BseJI GATNNNNATC 1 cut(s) 815
BseLI CCNNNNNNNGG 3 cut(s) 479, 763, 1319
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 986, 1397
BseNI ACTGG 3 cut(s) 573, 1006, 1167
BseSI GKGCMC 1 cut(s) 1336
BseXI GCAGC 4 cut(s) 209, 670, 803, 1044
Bsh1236I CGCG 2 cut(s) 36, 38
BshFI GGCC 1 cut(s) 756
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 1336
BsiHKCI CYCGRG 1 cut(s) 1339
BsiSI CCGG 2 cut(s) 217, 1446
BslI CCNNNNNNNGG 3 cut(s) 479, 763, 1319
BsmAI GTCTC 2 cut(s) 884, 1473
BsmBI CGTCTC 1 cut(s) 884
BsmI GAATGC 2 cut(s) 95, 457
BsnI GGCC 1 cut(s) 756
BsoBI CYCGRG 1 cut(s) 1339
Bsp1286I GDGCHC 2 cut(s) 778, 1336
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 2 cut(s) 985, 1396
BspDI ATCGAT 1 cut(s) 69
BspFNI CGCG 2 cut(s) 36, 38
BspLI GGNNCC 3 cut(s) 264, 1032, 1444
BspMAI CTGCAG 1 cut(s) 1422
BspOI GCTAGC 1 cut(s) 1277
BspPI GGATC 6 cut(s) 227, 257, 270, 463, 494, 1303
BspTI CTTAAG 1 cut(s) 1037
BsrDI GCAATG 1 cut(s) 61
BsrI ACTGG 3 cut(s) 573, 1006, 1167
BssECI CCNNGG 3 cut(s) 266, 459, 762
BssNI GRCGYC 1 cut(s) 858
BssSI CACGAG 1 cut(s) 108
BssT1I CCWWGG 2 cut(s) 459, 762
Bst2BI CACGAG 1 cut(s) 108
Bst2UI CCWGG 3 cut(s) 267, 1062, 1109
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 1254
BstACI GRCGYC 1 cut(s) 858
BstAFI CTTAAG 1 cut(s) 1037
BstC8I GCNNGC 3 cut(s) 23, 276, 1275
BstDEI CTNAG 7 cut(s) 357, 465, 744, 779, 883, 972, 1383
BstF5I GGATG 2 cut(s) 844, 1206
BstFNI CGCG 2 cut(s) 36, 38
BstH2I RGCGCY 2 cut(s) 217, 786
BstHHI GCGC 3 cut(s) 38, 216, 785
BstMAI GTCTC 2 cut(s) 884, 1473
BstMWI GCNNNNNNNGC 6 cut(s) 22, 461, 728, 782, 791, 1410
BstNI CCWGG 3 cut(s) 267, 1062, 1109
BstSCI CCNGG 3 cut(s) 265, 1060, 1107
BstSFI CTRYAG 1 cut(s) 1418
BstSLI GKGCMC 1 cut(s) 1336
BstUI CGCG 2 cut(s) 36, 38
BstV1I GCAGC 4 cut(s) 209, 670, 803, 1044
BstX2I RGATCY 5 cut(s) 190, 262, 468, 643, 1308
BstYI RGATCY 5 cut(s) 190, 262, 468, 643, 1308
Bsu15I ATCGAT 1 cut(s) 69
BsuI GTATCC 2 cut(s) 380, 406
BsuRI GGCC 1 cut(s) 756
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 2 cut(s) 844, 1206
BtsIMutI CAGTG 1 cut(s) 580
Cac8I GCNNGC 3 cut(s) 23, 276, 1275
CfoI GCGC 3 cut(s) 38, 216, 785
Cfr13I GGNCC 2 cut(s) 1031, 1372
ClaI ATCGAT 1 cut(s) 69
CseI GACGC 1 cut(s) 605
Csp6I GTAC 5 cut(s) 354, 932, 1000, 1126, 1264
CspCI CAANNNNNGTGG 2 cut(s) 751, 786
CviAII CATG 8 cut(s) 758, 1027, 1094, 1129, 1348, 1363, 1408, 1456
CviQI GTAC 5 cut(s) 354, 932, 1000, 1126, 1264
DdeI CTNAG 7 cut(s) 357, 465, 744, 779, 883, 972, 1383
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 1254
EarI CTCTTC 1 cut(s) 1254
EciI GGCGGA 2 cut(s) 26, 40
Eco130I CCWWGG 2 cut(s) 459, 762
Eco24I GRGCYC 1 cut(s) 778
Eco47I GGWCC 2 cut(s) 1031, 1372
Eco47III AGCGCT 1 cut(s) 784
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 1339
EcoO109I RGGNCCY 1 cut(s) 1372
EcoRII CCWGG 3 cut(s) 265, 1060, 1107
EcoT14I CCWWGG 2 cut(s) 459, 762
EcoT38I GRGCYC 1 cut(s) 778
ErhI CCWWGG 2 cut(s) 459, 762
Esp3I CGTCTC 1 cut(s) 884
FaeI CATG 8 cut(s) 761, 1030, 1097, 1132, 1351, 1366, 1411, 1459
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FatI CATG 8 cut(s) 757, 1026, 1093, 1128, 1347, 1362, 1407, 1455
FauI CCCGC 2 cut(s) 14, 41
FauNDI CATATG 1 cut(s) 834
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 5 cut(s) 198, 684, 792, 1058, 1405
FokI GGATG 2 cut(s) 851, 1213
FriOI GRGCYC 1 cut(s) 778
Fsp4HI GCNGC 5 cut(s) 198, 684, 792, 1058, 1405
FspBI CTAG 4 cut(s) 17, 194, 303, 1274
GlaI GCGC 3 cut(s) 37, 215, 784
GluI GCNGC 5 cut(s) 198, 684, 792, 1058, 1405
HaeII RGCGCY 2 cut(s) 217, 786
HaeIII GGCC 1 cut(s) 756
HapII CCGG 2 cut(s) 217, 1446
HgaI GACGC 1 cut(s) 605
HhaI GCGC 3 cut(s) 38, 216, 785
Hin1I GRCGYC 1 cut(s) 858
Hin1II CATG 8 cut(s) 761, 1030, 1097, 1132, 1351, 1366, 1411, 1459
Hin6I GCGC 3 cut(s) 36, 214, 783
HinP1I GCGC 3 cut(s) 36, 214, 783
HindIII AAGCTT 1 cut(s) 594
HinfI GANTC 6 cut(s) 494, 906, 1142, 1193, 1302, 1423
HpaII CCGG 2 cut(s) 217, 1446
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 3 cut(s) 245, 1034, 1334
Hpy188I TCNGA 5 cut(s) 127, 378, 499, 821, 1386
Hpy188III TCNNGA 7 cut(s) 185, 903, 959, 1012, 1306, 1339, 1341
Hpy8I GTNNAC 3 cut(s) 245, 1034, 1334
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 3 cut(s) 533, 858, 986
HpyCH4V TGCA 5 cut(s) 157, 686, 791, 1334, 1420
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 461, 728, 782, 791, 1410
HpyF3I CTNAG 7 cut(s) 357, 465, 744, 779, 883, 972, 1383
HpySE526I ACGT 3 cut(s) 533, 858, 986
Hsp92I GRCGYC 1 cut(s) 858
Hsp92II CATG 8 cut(s) 761, 1030, 1097, 1132, 1351, 1366, 1411, 1459
HspAI GCGC 3 cut(s) 36, 214, 783
Ksp22I TGATCA 1 cut(s) 691
LmnI GCTCC 4 cut(s) 13, 289, 728, 1287
Lsp1109I GCAGC 4 cut(s) 209, 670, 803, 1044
LweI GCATC 4 cut(s) 144, 1071, 1194, 1377
MaeI CTAG 4 cut(s) 17, 194, 303, 1274
MaeII ACGT 3 cut(s) 533, 858, 986
MaeIII GTNAC 4 cut(s) 58, 203, 977, 1194
MboII GAAGA 6 cut(s) 200, 391, 620, 1126, 1271, 1291
MflI RGATCY 5 cut(s) 190, 262, 468, 643, 1308
MhlI GDGCHC 2 cut(s) 778, 1336
MluCI AATT 5 cut(s) 63, 510, 735, 993, 1065
MlyI GAGTC 3 cut(s) 1202, 1296, 1432
MmeI TCCRAC 4 cut(s) 240, 301, 706, 1236
MnlI CCTC 8 cut(s) 514, 919, 1036, 1211, 1221, 1263, 1385, 1392
MseI TTAA 5 cut(s) 168, 537, 708, 1038, 1188
MslI CAYNNNNRTG 2 cut(s) 837, 1133
MspA1I CMGCKG 2 cut(s) 200, 442
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 2 cut(s) 217, 1446
MspR9I CCNGG 3 cut(s) 267, 1062, 1109
Mva1269I GAATGC 2 cut(s) 95, 457
MvaI CCWGG 3 cut(s) 267, 1062, 1109
MvnI CGCG 2 cut(s) 36, 38
MwoI GCNNNNNNNGC 6 cut(s) 22, 461, 728, 782, 791, 1410
NdeI CATATG 1 cut(s) 834
NheI GCTAGC 1 cut(s) 1273
NlaIII CATG 8 cut(s) 761, 1030, 1097, 1132, 1351, 1366, 1411, 1459
NlaIV GGNNCC 3 cut(s) 264, 1032, 1444
NmuCI GTSAC 3 cut(s) 58, 203, 1194
PaeR7I CTCGAG 1 cut(s) 1339
PctI GAATGC 2 cut(s) 95, 457
PfeI GAWTC 3 cut(s) 494, 906, 1142
PflMI CCANNNNNTGG 1 cut(s) 1319
PkrI GCNGC 5 cut(s) 199, 685, 793, 1059, 1406
PleI GAGTC 3 cut(s) 1201, 1296, 1431
PpsI GAGTC 3 cut(s) 1201, 1296, 1431
PpuMI RGGWCCY 1 cut(s) 1372
Psp5II RGGWCCY 1 cut(s) 1372
Psp6I CCWGG 3 cut(s) 265, 1060, 1107
PspGI CCWGG 3 cut(s) 265, 1060, 1107
PspN4I GGNNCC 3 cut(s) 264, 1032, 1444
PspPI GGNCC 2 cut(s) 1031, 1372
PspPPI RGGWCCY 1 cut(s) 1372
PsrI GAACNNNNNNTAC 2 cut(s) 992, 1024
PstI CTGCAG 1 cut(s) 1422
PsuI RGATCY 5 cut(s) 190, 262, 468, 643, 1308
PvuII CAGCTG 2 cut(s) 200, 442
RsaI GTAC 5 cut(s) 355, 933, 1001, 1127, 1265
RsaNI GTAC 5 cut(s) 354, 932, 1000, 1126, 1264
RseI CAYNNNNRTG 2 cut(s) 837, 1133
SaqAI TTAA 5 cut(s) 168, 537, 708, 1038, 1188
SatI GCNGC 5 cut(s) 198, 684, 792, 1058, 1405
Sau96I GGNCC 2 cut(s) 1031, 1372
SchI GAGTC 3 cut(s) 1202, 1296, 1432
ScrFI CCNGG 3 cut(s) 267, 1062, 1109
SduI GDGCHC 2 cut(s) 778, 1336
SfaNI GCATC 4 cut(s) 144, 1071, 1194, 1377
SfcI CTRYAG 1 cut(s) 1418
Sfr274I CTCGAG 1 cut(s) 1339
SinI GGWCC 2 cut(s) 1031, 1372
SlaI CTCGAG 1 cut(s) 1339
SmiMI CAYNNNNRTG 2 cut(s) 837, 1133
SmlI CTYRAG 5 cut(s) 699, 1037, 1304, 1339, 1391
SmoI CTYRAG 5 cut(s) 699, 1037, 1304, 1339, 1391
Sse9I AATT 5 cut(s) 63, 510, 735, 993, 1065
SspI AATATT 1 cut(s) 166
SspMI CTAG 4 cut(s) 17, 194, 303, 1274
StyD4I CCNGG 3 cut(s) 265, 1060, 1107
StyI CCWWGG 2 cut(s) 459, 762
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 3 cut(s) 536, 861, 989
TaqI TCGA 3 cut(s) 69, 435, 1340
TasI AATT 5 cut(s) 63, 510, 735, 993, 1065
TatI WGTACW 2 cut(s) 353, 1263
TauI GCSGC 1 cut(s) 1407
TfiI GAWTC 3 cut(s) 494, 906, 1142
Tru1I TTAA 5 cut(s) 168, 537, 708, 1038, 1188
Tru9I TTAA 5 cut(s) 168, 537, 708, 1038, 1188
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 3 cut(s) 58, 203, 1194
TseI GCWGC 4 cut(s) 197, 683, 791, 1057
Tsp45I GTSAC 3 cut(s) 58, 203, 1194
TspDTI ATGAA 6 cut(s) 102, 123, 1173, 1310, 1364, 1445
TspGWI ACGGA 2 cut(s) 506, 1213
TspRI CASTG 1 cut(s) 580
Van91I CCANNNNNTGG 1 cut(s) 1319
Vha464I CTTAAG 1 cut(s) 1037
VneI GTGCAC 1 cut(s) 1332
VpaK11BI GGWCC 2 cut(s) 1031, 1372
XcmI CCANNNNNNNNNTGG 1 cut(s) 764
XhoI CTCGAG 1 cut(s) 1339
XspI CTAG 4 cut(s) 17, 194, 303, 1274
ZraI GACGTC 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.