MD17G1097300.v1.1

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009104
Physical Location & Seq
Forward (+)
8240182 .. 8241129
948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1097300.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGTGTTGGCGGAATTAGGTGGGAGCATAACCCGTGCCCTCCAGCAGATGATCAAGGCGACGGTGGTCGACGAGAAGGTTCTGAACGACTGCCTCAACGAAATCAGCCGCGCTCTGCTCCAATCCGACGTCCAATTCAACCTTGTTCGCGACATGCAGATCAACATTAAAAAGATTGTCAATCTGTCCGATCTCGCTGCCGGTCACAACAAGCGCAAGATCATTCAGCAAGCCGTGTTCGATCAACTCTGCCAAATGCTGCAAGATCCAGCAGGGAAACTCGATCAGCTTGTACCCAAAAAAGGGAAGAAACCGAGTCAAGTTGTCATGTTTGTTGGTTTACAAGGGTCTGGAAAAACCACAACGTCTACAAAATACGCGTATTATCATAAGAACAAAGGTTGGAAACCCGCCTTAGTGTGTGCGGACACATTCAGAGCTGGCGCTTTCGATCAGCTGAAGCAAAACGCTGCCAAGGCTAAGGTTCCGTTTTATGGAAGTTACACAGAGATGGACCCTGTAAAGATTGCTGTTGAAGGTGTGGAGAGGTTAAAAAACGATCAGAACAATAATTTTGATCTCGTAGTTGTGGATACTAGTGGGAGGCACAAGCAAGAAGCTGCTCTTTTTGAAGAGATGCGTCAACTTTCCGAAGCAATTAGTCCGGACCTTGTTGTGTTTGTTATGGATGCGAGTATTGGTCAGTCTGCTTTTCATCAAGCGCAAGCGTTTAAGCAAAGCGTTACTGTTGGAGCTGTCATTGTTACAAAAATGGATGGTCACGCAAAGGGTGGCGGCGCTCTTAGCGCTGTTGCAGCAACAAAGAGTCCTGTCATATTTATTGGAACGGGAGAGCATATGGATCAGTTGGAAGCCTTCGATGTGAAACCCTTTTGTCCGGCGTCTGTTAGGCATGGGGGACTTGTCTGGACTTGTGAACAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

316

Amino Acids

34.38

Weight (kDa)

8.47

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 6 - 83 2.8e-12 SRP54-type protein, helical bundle domain
SRP54 PF00448 107 - 298 1.8e-71 SRP54-type protein, GTPase domain
cobW PF02492 108 - 261 8.3e-07 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccI GTMKAC 2 cut(s) 69, 368
AccII CGCG 3 cut(s) 111, 150, 380
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 5 cut(s) 11, 109, 411, 425, 795
AclWI GGATC 2 cut(s) 260, 870
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 2 cut(s) 129, 902
AfaI GTAC 1 cut(s) 294
AfeI AGCGCT 1 cut(s) 808
AfiI CCNNNNNNNGG 3 cut(s) 302, 303, 494
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 3 cut(s) 139, 536, 632
AhlI ACTAGT 1 cut(s) 596
AluBI AGCT 5 cut(s) 289, 440, 457, 620, 755
AluI AGCT 5 cut(s) 289, 440, 457, 620, 755
AlwI GGATC 2 cut(s) 260, 870
Aor13HI TCCGGA 1 cut(s) 664
Aor51HI AGCGCT 1 cut(s) 808
ApeKI GCWGC 5 cut(s) 197, 259, 470, 620, 815
AspLEI GCGC 6 cut(s) 113, 216, 446, 724, 800, 809
AspS9I GGNCC 2 cut(s) 514, 667
AvaII GGWCC 2 cut(s) 514, 667
BaeGI GKGCMC 1 cut(s) 40
BbvI GCAGC 5 cut(s) 184, 246, 457, 607, 827
BccI CCATC 2 cut(s) 505, 770
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciVI GTATCC 1 cut(s) 586
BclI TGATCA 1 cut(s) 51
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 1 cut(s) 597
BfoI RGCGCY 3 cut(s) 447, 801, 810
BfuI GTATCC 1 cut(s) 586
BisI GCNGC 7 cut(s) 109, 198, 260, 471, 621, 796, 816
BlsI GCNGC 7 cut(s) 110, 199, 261, 472, 622, 797, 817
Bme18I GGWCC 2 cut(s) 514, 667
BmgT120I GGNCC 2 cut(s) 514, 667
BmiI GGNNCC 2 cut(s) 486, 516
BmsI GCATC 2 cut(s) 627, 679
BoxI GACNNNNGTC 1 cut(s) 65
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 480
BsaHI GRCGYC 2 cut(s) 129, 902
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 303, 494
Bse118I RCCGGY 1 cut(s) 200
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 2 cut(s) 694, 781
BseLI CCNNNNNNNGG 3 cut(s) 302, 303, 494
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 5 cut(s) 184, 246, 457, 607, 827
Bsh1236I CGCG 3 cut(s) 111, 150, 380
BsiSI CCGG 3 cut(s) 201, 665, 899
BslFI GGGAC 1 cut(s) 933
BslI CCNNNNNNNGG 3 cut(s) 302, 303, 494
BsmFI GGGAC 1 cut(s) 933
Bsp1286I GDGCHC 1 cut(s) 40
Bsp13I TCCGGA 1 cut(s) 664
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 5 cut(s) 11, 109, 411, 425, 795
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 3 cut(s) 111, 150, 380
BspLI GGNNCC 2 cut(s) 486, 516
BspPI GGATC 2 cut(s) 260, 870
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 474
BssNI GRCGYC 2 cut(s) 129, 902
BssT1I CCWWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 64, 748
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 2 cut(s) 129, 902
BstC8I GCNNGC 3 cut(s) 231, 442, 726
BstDEI CTNAG 3 cut(s) 415, 480, 803
BstF5I GGATG 2 cut(s) 694, 781
BstFNI CGCG 3 cut(s) 111, 150, 380
BstH2I RGCGCY 3 cut(s) 447, 801, 810
BstHHI GCGC 6 cut(s) 113, 216, 446, 724, 800, 809
BstMWI GCNNNNNNNGC 4 cut(s) 476, 804, 806, 815
BstNSI RCATGY 1 cut(s) 157
BstPAI GACNNNNGTC 1 cut(s) 65
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 3 cut(s) 111, 150, 380
BstV1I GCAGC 5 cut(s) 184, 246, 457, 607, 827
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuI GTATCC 1 cut(s) 586
BtsCI GGATG 2 cut(s) 694, 781
BtuMI TCGCGA 1 cut(s) 150
Cac8I GCNNGC 3 cut(s) 231, 442, 726
CfoI GCGC 6 cut(s) 113, 216, 446, 724, 800, 809
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 514, 667
CseI GACGC 2 cut(s) 629, 891
Csp6I GTAC 1 cut(s) 293
CviAII CATG 3 cut(s) 154, 328, 914
CviJI RGCY 9 cut(s) 108, 233, 289, 440, 457, 479, 620, 755, 875
CviKI_1 RGCY 9 cut(s) 108, 233, 289, 440, 457, 479, 620, 755, 875
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 3 cut(s) 415, 480, 803
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 2 cut(s) 514, 667
Eco47III AGCGCT 1 cut(s) 808
Eco57I CTGAAG 1 cut(s) 479
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 3 cut(s) 157, 331, 917
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FaqI GGGAC 1 cut(s) 933
FatI CATG 3 cut(s) 153, 327, 913
FauI CCCGC 1 cut(s) 418
FauNDI CATATG 1 cut(s) 858
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 2 cut(s) 69, 368
Fnu4HI GCNGC 7 cut(s) 109, 198, 260, 471, 621, 796, 816
FokI GGATG 2 cut(s) 701, 788
Fsp4HI GCNGC 7 cut(s) 109, 198, 260, 471, 621, 796, 816
FspBI CTAG 1 cut(s) 597
GlaI GCGC 6 cut(s) 112, 215, 445, 723, 799, 808
GluI GCNGC 7 cut(s) 109, 198, 260, 471, 621, 796, 816
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 3 cut(s) 447, 801, 810
HapII CCGG 3 cut(s) 201, 665, 899
HgaI GACGC 2 cut(s) 629, 891
HhaI GCGC 6 cut(s) 113, 216, 446, 724, 800, 809
Hin1I GRCGYC 2 cut(s) 129, 902
Hin1II CATG 3 cut(s) 157, 331, 917
Hin6I GCGC 6 cut(s) 111, 214, 444, 722, 798, 807
HinP1I GCGC 6 cut(s) 111, 214, 444, 722, 798, 807
HincII GTYRAC 2 cut(s) 70, 644
HindII GTYRAC 2 cut(s) 70, 644
HinfI GANTC 2 cut(s) 316, 826
HpaII CCGG 3 cut(s) 201, 665, 899
Hpy166II GTNNAC 5 cut(s) 70, 341, 369, 644, 938
Hpy188I TCNGA 6 cut(s) 84, 127, 190, 437, 564, 652
Hpy188III TCNNGA 4 cut(s) 149, 351, 665, 928
Hpy8I GTNNAC 5 cut(s) 70, 341, 369, 644, 938
Hpy99I CGWCG 3 cut(s) 64, 74, 131
HpyAV CCTTC 3 cut(s) 70, 530, 886
HpyCH4III ACNGT 2 cut(s) 64, 748
HpyCH4IV ACGT 2 cut(s) 129, 365
HpyCH4V TGCA 3 cut(s) 157, 262, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 476, 804, 806, 815
HpyF3I CTNAG 3 cut(s) 415, 480, 803
HpySE526I ACGT 2 cut(s) 129, 365
Hsp92I GRCGYC 2 cut(s) 129, 902
Hsp92II CATG 3 cut(s) 157, 331, 917
HspAI GCGC 6 cut(s) 111, 214, 444, 722, 798, 807
Kpn2I TCCGGA 1 cut(s) 664
Ksp22I TGATCA 1 cut(s) 51
LmnI GCTCC 3 cut(s) 24, 123, 752
Lsp1109I GCAGC 5 cut(s) 184, 246, 457, 607, 827
LweI GCATC 2 cut(s) 627, 679
MaeI CTAG 1 cut(s) 597
MaeII ACGT 2 cut(s) 129, 365
MaeIII GTNAC 5 cut(s) 203, 500, 742, 763, 779
MboII GAAGA 2 cut(s) 319, 644
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 4 cut(s) 14, 134, 571, 657
MluI ACGCGT 1 cut(s) 378
MlyI GAGTC 2 cut(s) 325, 835
MmeI TCCRAC 4 cut(s) 150, 383, 730, 849
MnlI CCTC 4 cut(s) 50, 104, 540, 597
MroI TCCGGA 1 cut(s) 664
MseI TTAA 3 cut(s) 168, 551, 732
MslI CAYNNNNRTG 1 cut(s) 509
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 3 cut(s) 201, 665, 899
MvnI CGCG 3 cut(s) 111, 150, 380
MwoI GCNNNNNNNGC 4 cut(s) 476, 804, 806, 815
NdeI CATATG 1 cut(s) 858
NlaIII CATG 3 cut(s) 157, 331, 917
NlaIV GGNNCC 2 cut(s) 486, 516
NmuCI GTSAC 2 cut(s) 203, 779
NruI TCGCGA 1 cut(s) 150
NspI RCATGY 1 cut(s) 157
PkrI GCNGC 7 cut(s) 110, 199, 261, 472, 622, 797, 817
PleI GAGTC 2 cut(s) 324, 834
PpsI GAGTC 2 cut(s) 324, 834
PshAI GACNNNNGTC 1 cut(s) 65
PspN4I GGNNCC 2 cut(s) 486, 516
PspPI GGNCC 2 cut(s) 514, 667
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 457
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 509
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 3 cut(s) 168, 551, 732
SatI GCNGC 7 cut(s) 109, 198, 260, 471, 621, 796, 816
Sau96I GGNCC 2 cut(s) 514, 667
SchI GAGTC 2 cut(s) 325, 835
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 2 cut(s) 627, 679
SinI GGWCC 2 cut(s) 514, 667
SmiMI CAYNNNNRTG 1 cut(s) 509
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 4 cut(s) 14, 134, 571, 657
SsiI CCGC 5 cut(s) 11, 109, 411, 425, 795
SspMI CTAG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 64, 748
TaiI ACGT 2 cut(s) 132, 368
TaqI TCGA 5 cut(s) 69, 240, 282, 450, 879
TasI AATT 4 cut(s) 14, 134, 571, 657
TauI GCSGC 2 cut(s) 111, 798
Tru1I TTAA 3 cut(s) 168, 551, 732
Tru9I TTAA 3 cut(s) 168, 551, 732
TseFI GTSAC 2 cut(s) 203, 779
TseI GCWGC 5 cut(s) 197, 259, 470, 620, 815
Tsp45I GTSAC 2 cut(s) 203, 779
TspDTI ATGAA 1 cut(s) 704
TspGWI ACGGA 1 cut(s) 477
VpaK11BI GGWCC 2 cut(s) 514, 667
XceI RCATGY 1 cut(s) 157
XmiI GTMKAC 2 cut(s) 69, 368
XspI CTAG 1 cut(s) 597
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.