Rorug06G0369800

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008275
Physical Location & Seq
Forward (+)
51650332 .. 51652228
1897 bp
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UTR
Exon/CDS
Intron
Rorug06G0369800.1

Sequence Viewer

Length: 1251 bp
ATGAAGAAAAGAAAAAGAATGGAGATGGAGTTGGATAGAATAAGCAACTTACCAAGTGATGTTACAGAAAAGATATTGTTGTGCTTGCCAGTTAGGGATGCAGTGAGGACGAGTGTTTTATCAACTAAGTGGAGGTACAAATCGGCTATGCTACCACATCTGGTGTTTGATCCCCAGTCTATCTCAACTCAACACCAAACATTTGCTAATGTTGTTGATCATTTACTCTTAAGTCACTTTGGCCCCGTATACAAGTTCAAGCTTGCTCGAGAACATGTATCCTTTAGAGATATTGATCGGTGGATACTTTACATGTCAAGAAACTCCATCAAAGAGTTCATACTTGAAATCTTGGGACGGCAGCGCTATAAGGTGCCTTCATCTATGTTTTCATGTCAAGATTTGACTTATTTAGAGTTGCATAGATGTTTGCTACAACCGCCGTCCACATTCACAGGCTTCAAAAGTTTGAAAAGCCTAAAAATTGTTCGTGTTACTCTGACGGATGATGTGCTTGAAAATCTGATTGTTCACTGTCCTCTACTTGAGCGGTTGACTGTGGAGTGGTGTTATGATTTCAGCCAGCTCAATATCAATGCACCAAATCTCCAGGTTCTTCGCTTTATGGGTGATTTTGAGGATGTTAATATATTAAATACCTCAAATCTCGTTGATGTTTTCATTTGGCTACGCAAGTTTGATCATAGACGGGGTCCTTGCAGATCTAGTAATCTGCTCAAGTTATTTGCTCAACTTCCCAATATTCACCGTCTTTACATGAACTCCAAATTTACAAAGTATTTGGCTGCTGGCTCCTTGCTGGAAAAGCTGCCTAAACCGTGTTTACATCTGCAATTTCTTATTGTAAGAGTAGGGATTGATGATGTTAAGGATATTATAACTGTTATTTGCCTTCTGAGAATCTCCCCTGCTCTGGAAGAATTAGAAGTTTTTGCTTTTTGCCGCCAAACATATGAGGCCGAAGTGGGAAAAGTGAACTCTTGGGTTGATGACAACCAGAGTTGCTCGTTCACTCAGCTGCGACATGTGAAAATACACTGCTCTCAGGCTGTTGAAGGTGCACTAGATTTGATCAGATTTCTGCTTTTAAGTTCACCTGCACTTGAGACAATGACAGTATATAAACATCCTTCCACTCCTCTTGATGGTTATGATCTAGCAAAAAAGTTACTCCGGTTTAGGCGTGCATCACTGAATTTAGAGATAATTCTAAGTGATCTAGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

416

Amino Acids

48.2

Weight (kDa)

9.02

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 47 7.3e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 90 - 208 4.1e-15 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 98 - 317 4.9e-21 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 196 - 380 1.2e-06 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AarI CACCTGC 1 cut(s) 1126
Acc36I ACCTGC 1 cut(s) 1126
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 550
AccI GTMKAC 1 cut(s) 249
AciI CCGC 3 cut(s) 440, 550, 964
AclWI GGATC 1 cut(s) 164
AcsI RAATTY 2 cut(s) 788, 1216
AfaI GTAC 1 cut(s) 137
AfeI AGCGCT 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 934, 1166
AflII CTTAAG 1 cut(s) 229
AflIII ACRYGT 3 cut(s) 274, 312, 1045
AgsI TTSAA 6 cut(s) 259, 347, 463, 472, 518, 1076
AjnI CCWGG 1 cut(s) 609
AluBI AGCT 4 cut(s) 262, 586, 829, 1039
AluI AGCT 4 cut(s) 262, 586, 829, 1039
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 1 cut(s) 1121
Alw44I GTGCAC 1 cut(s) 1080
AlwI GGATC 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 267
Aor51HI AGCGCT 1 cut(s) 365
AoxI GGCC 2 cut(s) 241, 978
ApaLI GTGCAC 1 cut(s) 1080
ApeKI GCWGC 4 cut(s) 361, 806, 829, 1039
ApoI RAATTY 2 cut(s) 788, 1216
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 242, 713
AsuHPI GGTGA 3 cut(s) 641, 758, 1107
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 1084
BanI GGYRCC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 4 cut(s) 373, 793, 816, 1026
BccI CCATC 3 cut(s) 19, 335, 1160
BceAI ACGGC 2 cut(s) 374, 427
BciT130I CCWGG 1 cut(s) 611
BciVI GTATCC 2 cut(s) 289, 297
BclI TGATCA 3 cut(s) 217, 700, 1092
BcoDI GTCTC 1 cut(s) 1121
BfaI CTAG 4 cut(s) 726, 1085, 1178, 1241
BfoI RGCGCY 1 cut(s) 367
BfrI CTTAAG 1 cut(s) 229
BfuAI ACCTGC 1 cut(s) 1126
BfuI GTATCC 2 cut(s) 289, 297
BglII AGATCT 1 cut(s) 722
BisI GCNGC 5 cut(s) 362, 807, 830, 964, 1040
BlsI GCNGC 5 cut(s) 363, 808, 831, 965, 1041
Bme1390I CCNGG 1 cut(s) 611
Bme18I GGWCC 1 cut(s) 713
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 242, 713
BmiI GGNNCC 4 cut(s) 244, 375, 714, 814
BmrFI CCNGG 1 cut(s) 611
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 2 cut(s) 88, 1217
BmuI ACTGGG 1 cut(s) 169
BpmI CTGGAG 1 cut(s) 593
BpuEI CTTGAG 3 cut(s) 566, 722, 1145
BsaBI GATNNNNATC 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 1194
BsaXI ACNNNNNCTCC 6 cut(s) 14, 44, 591, 621, 767, 797
Bsc4I CCNNNNNNNGG 2 cut(s) 934, 1166
Bse1I ACTGG 2 cut(s) 89, 175
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 1 cut(s) 611
BseGI GGATG 4 cut(s) 103, 511, 646, 1147
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 2 cut(s) 934, 1166
BseMII CTCAG 3 cut(s) 908, 1049, 1079
BseNI ACTGG 2 cut(s) 89, 175
BseRI GAGGAG 1 cut(s) 1149
BseSI GKGCMC 1 cut(s) 1084
BseXI GCAGC 4 cut(s) 373, 793, 816, 1026
BsgI GTGCAG 1 cut(s) 1104
BshFI GGCC 2 cut(s) 243, 980
BshNI GGYRCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 1 cut(s) 1195
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 934, 1166
BsmAI GTCTC 1 cut(s) 1121
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 2 cut(s) 243, 980
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 1084
Bsp143I GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
BspACI CCGC 3 cut(s) 440, 550, 964
BspANI GGCC 2 cut(s) 243, 980
BspCNI CTCAG 3 cut(s) 909, 1048, 1078
BspLI GGNNCC 4 cut(s) 244, 375, 714, 814
BspMI ACCTGC 1 cut(s) 1126
BspPI GGATC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 373
BspTI CTTAAG 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 550
BsrI ACTGG 2 cut(s) 89, 175
BssMI GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
BssNAI GTATAC 1 cut(s) 250
Bst1107I GTATAC 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 6 cut(s) 536, 559, 770, 840, 904, 1138
BstAFI CTTAAG 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 86, 264, 584, 811, 1206
BstDEI CTNAG 5 cut(s) 126, 917, 1035, 1065, 1232
BstF5I GGATG 4 cut(s) 103, 511, 646, 1147
BstH2I RGCGCY 1 cut(s) 367
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 8 cut(s) 172, 220, 298, 703, 725, 1095, 1177, 1240
BstMAI GTCTC 1 cut(s) 1121
BstMBI GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
BstMWI GCNNNNNNNGC 2 cut(s) 439, 826
BstNI CCWGG 1 cut(s) 611
BstNSI RCATGY 3 cut(s) 278, 316, 1049
BstSCI CCNGG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 1084
BstV1I GCAGC 4 cut(s) 373, 793, 816, 1026
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BstZ17I GTATAC 1 cut(s) 250
BsuI GTATCC 2 cut(s) 289, 297
BsuRI GGCC 2 cut(s) 243, 980
BtsCI GGATG 4 cut(s) 103, 511, 646, 1147
BtsI GCAGTG 2 cut(s) 108, 1057
BtsIMutI CAGTG 4 cut(s) 108, 532, 1057, 1211
BveI ACCTGC 1 cut(s) 1126
Cac8I GCNNGC 5 cut(s) 86, 264, 584, 811, 1206
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 242, 713
Csp6I GTAC 1 cut(s) 136
CspCI CAANNNNNGTGG 2 cut(s) 1144, 1179
CviAII CATG 5 cut(s) 275, 313, 393, 778, 1046
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 5 cut(s) 126, 917, 1035, 1065, 1232
DpnI GATC 8 cut(s) 171, 219, 297, 702, 724, 1094, 1176, 1239
DpnII GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
Eco47I GGWCC 1 cut(s) 713
Eco47III AGCGCT 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 609
FaeI CATG 5 cut(s) 278, 316, 396, 781, 1049
FaqI GGGAC 1 cut(s) 369
FatI CATG 5 cut(s) 274, 312, 392, 777, 1045
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 3 cut(s) 217, 700, 1092
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 362, 807, 830, 964, 1040
FokI GGATG 4 cut(s) 110, 518, 653, 1134
Fsp4HI GCNGC 5 cut(s) 362, 807, 830, 964, 1040
FspBI CTAG 4 cut(s) 726, 1085, 1178, 1241
GlaI GCGC 1 cut(s) 365
GluI GCNGC 5 cut(s) 362, 807, 830, 964, 1040
GsuI CTGGAG 1 cut(s) 593
HaeII RGCGCY 1 cut(s) 367
HaeIII GGCC 2 cut(s) 243, 980
HapII CCGG 1 cut(s) 1195
HhaI GCGC 1 cut(s) 366
Hin1II CATG 5 cut(s) 278, 316, 396, 781, 1049
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 1 cut(s) 921
HpaII CCGG 1 cut(s) 1195
HphI GGTGA 3 cut(s) 641, 758, 1107
Hpy166II GTNNAC 9 cut(s) 250, 447, 532, 555, 845, 997, 1032, 1082, 1115
Hpy188I TCNGA 4 cut(s) 501, 525, 918, 1097
Hpy188III TCNNGA 5 cut(s) 269, 318, 398, 935, 1163
Hpy8I GTNNAC 9 cut(s) 250, 447, 532, 555, 845, 997, 1032, 1082, 1115
HpyAV CCTTC 4 cut(s) 387, 923, 1070, 1161
HpyCH4III ACNGT 6 cut(s) 536, 559, 770, 840, 904, 1138
HpyCH4V TGCA 8 cut(s) 101, 421, 599, 720, 853, 1082, 1121, 1208
HpyF10VI GCNNNNNNNGC 2 cut(s) 439, 826
HpyF3I CTNAG 5 cut(s) 126, 917, 1035, 1065, 1232
Hsp92II CATG 5 cut(s) 278, 316, 396, 781, 1049
HspAI GCGC 1 cut(s) 364
Ksp22I TGATCA 3 cut(s) 217, 700, 1092
Kzo9I GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
LmnI GCTCC 1 cut(s) 818
Lsp1109I GCAGC 4 cut(s) 373, 793, 816, 1026
LweI GCATC 2 cut(s) 88, 1217
MaeI CTAG 4 cut(s) 726, 1085, 1178, 1241
MaeIII GTNAC 4 cut(s) 61, 233, 493, 1188
MalI GATC 8 cut(s) 171, 219, 297, 702, 724, 1094, 1176, 1239
MbiI CCGCTC 1 cut(s) 550
MboI GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
MboII GAAGA 3 cut(s) 16, 608, 950
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 1 cut(s) 1084
MluCI AATT 6 cut(s) 483, 788, 854, 941, 1216, 1227
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 7 cut(s) 99, 126, 549, 631, 670, 970, 1170
MseI TTAA 5 cut(s) 230, 645, 653, 888, 1109
MspA1I CMGCKG 1 cut(s) 1039
MspCI CTTAAG 1 cut(s) 229
MspI CCGG 1 cut(s) 1195
MspR9I CCNGG 1 cut(s) 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 2 cut(s) 439, 826
NdeI CATATG 1 cut(s) 973
NdeII GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
NlaIII CATG 5 cut(s) 278, 316, 396, 781, 1049
NlaIV GGNNCC 4 cut(s) 244, 375, 714, 814
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 3 cut(s) 278, 316, 1049
PaeR7I CTCGAG 1 cut(s) 267
PaqCI CACCTGC 1 cut(s) 1126
PciI ACATGT 3 cut(s) 274, 312, 1045
PfeI GAWTC 1 cut(s) 921
PflFI GACNNNGTC 1 cut(s) 711
PkrI GCNGC 5 cut(s) 363, 808, 831, 965, 1041
PpuMI RGGWCCY 1 cut(s) 713
PscI ACATGT 3 cut(s) 274, 312, 1045
PsiI TTATAA 1 cut(s) 899
Psp5II RGGWCCY 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 4 cut(s) 244, 375, 714, 814
PspPI GGNCC 2 cut(s) 242, 713
PspPPI RGGWCCY 1 cut(s) 713
PsuI RGATCY 1 cut(s) 722
PsyI GACNNNGTC 1 cut(s) 711
PvuII CAGCTG 1 cut(s) 1039
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SaqAI TTAA 5 cut(s) 230, 645, 653, 888, 1109
SatI GCNGC 5 cut(s) 362, 807, 830, 964, 1040
Sau3AI GATC 8 cut(s) 169, 217, 295, 700, 722, 1092, 1174, 1237
Sau96I GGNCC 2 cut(s) 242, 713
ScrFI CCNGG 1 cut(s) 611
SduI GDGCHC 1 cut(s) 1084
SfaNI GCATC 2 cut(s) 88, 1217
Sfr274I CTCGAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 713
SlaI CTCGAG 1 cut(s) 267
SmlI CTYRAG 5 cut(s) 229, 267, 545, 737, 1124
SmoI CTYRAG 5 cut(s) 229, 267, 545, 737, 1124
Sse9I AATT 6 cut(s) 483, 788, 854, 941, 1216, 1227
SsiI CCGC 3 cut(s) 440, 550, 964
SspI AATATT 1 cut(s) 763
SspMI CTAG 4 cut(s) 726, 1085, 1178, 1241
StyD4I CCNGG 1 cut(s) 609
TaaI ACNGT 6 cut(s) 536, 559, 770, 840, 904, 1138
TaqI TCGA 1 cut(s) 268
TasI AATT 6 cut(s) 483, 788, 854, 941, 1216, 1227
TauI GCSGC 1 cut(s) 966
TfiI GAWTC 1 cut(s) 921
Tru1I TTAA 5 cut(s) 230, 645, 653, 888, 1109
Tru9I TTAA 5 cut(s) 230, 645, 653, 888, 1109
TscAI CASTG 4 cut(s) 108, 539, 1064, 1218
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 4 cut(s) 361, 806, 829, 1039
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 6 cut(s) 17, 328, 369, 381, 670, 794
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 4 cut(s) 108, 539, 1064, 1218
Tth111I GACNNNGTC 1 cut(s) 711
Vha464I CTTAAG 1 cut(s) 229
VneI GTGCAC 1 cut(s) 1080
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 2 cut(s) 788, 1216
XceI RCATGY 3 cut(s) 278, 316, 1049
XhoI CTCGAG 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 726, 1085, 1178, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.