Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0008275

Physical Location & Seq

Genomic Coordinates Forward (+)

65636172 .. 65640829

Gene Structure 4658 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G042020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGGCTCCTCGGAGGTTGGTGGTCCTCCTTCTAATTTACTCCGTCCTACTCAATTTTTGTGGCGCGTTTGCTTCAACCGCCGATGATCAACGCCGTCCGATGATCCTTCCTCTCCATCTCTCCTCCCCCGACGACGCTCGCCACCACCGGCGAGCCTTCGACGGTCGGCGTCTCCAGAGATCGGAGCCGAACGCGCACATGAGGCTCCACGATGATCTCCTTACCAACGGGTACTACACGACGCGGCTGTGGATTGGGACACCGCCTCAGATGTTCGCTCTGATTGTGGATACGGGGAGTACTGTCACTTATGTGCCTTGCTCTGACTGTGAAGCCTGCGGTAACCATCAGGACCCTAGGTTTCAACCCGATTTATCTAGCACGTACCAACCTGTAAAGTGTAATATCAATTGCAACTGTGATGATAAAGGAAACCAATGCACTTATGAGAGGCGCTATGCTGAGCAGAGCTCCAGCAGTGGTGTGCTTGGTGAGGATGTCATTTCTTTTGGCAATGAGAGTGCACTTGTGCCCCAGCGTGCTGTTTTTGGTTGCGAGAATATTGAAACCGGTGATCTTTACAGCCAACGTGCCGACGGCATAATGGGTTTGGGTCGTGGTCAGCTTAGCGTCATGGACCAGCTTGTTGATAAGGGTGTTATTAGTGATTCATTTTCATTGTGTTATGGAGGAATGGGTGTAGGTGGGGGTGCTATGGTTCTAGGTGGGATTAAATCCCCCCCGGATATGGTGTTTACCCATTCTGATCCCTTTCGCAGTCCATATTACAATATTGAACTGAAGGAGATACATGTGGCTGGAAAGGCTTTGAAGTTAAACCCGAAGGTGTTTGATGAAAAGCATGGAACTGTCTTGGATAGTGGAACGACATATGCATACTTTCCAAAGGATGCTTTTATTGCATTTAAGGATGCTATTATGAAGGAAACTCATTTCCTCAAACAAGTCCATGGTCCTGACCCAAATTATAAAGATATCTGTTTTTCAGGTGCTGGAAGGGATGTCACCCAACTATCAAAAGTTTTTCCACAGGTTGATATGATATTCAGCAAGGGACAGAAGTTTTCACTGTCTCCCGAGAACTATTTGTTTCCGCATACGAAGGTTAGCGGGGCTTATTGCCTGGGAATTTTTGAAAATGGGGATTCAACCACTCTTTTAGGAGGAATTCTTGTACGTAATACCCTTGTGACATACGATCGAGCGGACAATAAGATTGGCTTTTGGAAAACCAATTGTTCTGAACTATGGAAGAGTTTAAATCATGAGAATGCTCAGGCCCCATCTTCTAACGACGGGAATATGAGTGGAGGGATTGCTCCTGACATCGCCCCAGCTGGATTGCCTCAAACTGATCTTCCAGGTGAACTTCAAATAGGGCAGATAAGTTTCGATATGATGCTCAACATCAGCAACTCTATGAAACCCAACTTCACAGAACTTGCAGAGTCTATTGCCCATGAGCTGGAAGTCGATATTTCTCAGGTTCACTTGAGGAACTACACTATTTCAGGAAATGATTTACTCATTAAGTGGACTATCGTACCTGATGAATCTGCCAATAGCTTTACTACTACCAAAGCAATGAACATAATTCGGCGCTTAAGGGAACATCAGATGCAGCTTCCCCATAAATTTGGGAGTTATCAGCTGGTCAAATTGAAAGTTGAACCTCAAATAAAGCGGTCATGGTGGGAGCAACATTCTTGGATGGTGGCAGTTGGAGTTTCAGTTGCCTCGGTTCTTGGACTATTAGCATTAGGGACATGGTTGGTTTGGAGATACAGATCGCGACAGGGCATGACTTATGAGCCTGTTGATGGCGTTGTTGGTGCGGTTGTTGCAGAGCAAGAACTTCAGCCCCTTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005623 GO:0005886 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016020 GO:0016787 GO:0019538 GO:0030163 GO:0031224 GO:0031225 GO:0031226 GO:0043170 GO:0044238 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0070001 GO:0070011 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

630

Amino Acids

69.94

Weight (kDa)

6.07

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAXi_N	PF14543	79 - 243	1.4e-36	Xylanase inhibitor N-terminal
Asp	PF00026	79 - 415	5.5e-34	Eukaryotic aspartyl protease
TAXi_C	PF14541	263 - 415	7.1e-27	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43100 AT5G43100
fragaria_vesca	FvH4_2g33420
malus_domestica	MD08G1089200.v1.1 MD08G1089400.v1.1 MD15G1074000.v1.1
prunus_persica	Prupe.1G426800_v2.0.a1 Prupe.1G426800_v2.0.a1 Prupe.1G426800_v2.0.a1 Prupe.1G426800_v2.0.a1
pyrus_communis	pycom08g07250 pycom08g07270 pycom15g06910
rosa_chinensis	RchiOBHm_Chr6g0309111
rosa_laevigata	RLG00000010588
rosa_roxburghii	Rroxscaffold_7G00159640
rosa_rugosa	Rorug06G0369800
rosa_samantha	Rh6AG482600 Rh6BG491600 Rh6BG491700 Rh6CG496400 Rh6DG483300
rosa_wichuraiana	Rw6G042020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	990
AccB7I	CCANNNNNTGG	1 cut(s)	1486
AccBSI	CCGCTC	1 cut(s)	1226
AccII	CGCG	4 cut(s)	65, 194, 244, 1813
AciI	CCGC	9 cut(s)	78, 244, 263, 339, 1115, 1131, 1226, 1705, 1856
AclWI	GGATC	2 cut(s)	97, 761
AcsI	RAATTY	3 cut(s)	1149, 1188, 1655
AcuI	CTGAAG	2 cut(s)	821, 1862
AcyI	GRCGYC	1 cut(s)	169
AfaI	GTAC	5 cut(s)	233, 301, 386, 1197, 1566
AfiI	CCNNNNNNNGG	4 cut(s)	181, 748, 1486, 1841
AflII	CTTAAG	1 cut(s)	1624
AflIII	ACRYGT	1 cut(s)	811
AgeI	ACCGGT	1 cut(s)	569
AjnI	CCWGG	2 cut(s)	1143, 1381
AleI	CACNNNNGTG	1 cut(s)	311
AluBI	AGCT	8 cut(s)	471, 625, 643, 1358, 1486, 1587, 1645, 1672
AluI	AGCT	8 cut(s)	471, 625, 643, 1358, 1486, 1587, 1645, 1672
Alw21I	GWGCWC	2 cut(s)	473, 526
Alw26I	GTCTC	2 cut(s)	176, 1098
Alw44I	GTGCAC	1 cut(s)	522
AlwI	GGATC	2 cut(s)	97, 761
AlwNI	CAGNNNCTG	1 cut(s)	1013
Ama87I	CYCGRG	1 cut(s)	1097
AoxI	GGCC	1 cut(s)	1299
ApaLI	GTGCAC	1 cut(s)	522
ApeKI	GCWGC	1 cut(s)	1642
ApoI	RAATTY	3 cut(s)	1149, 1188, 1655
AsiGI	ACCGGT	1 cut(s)	569
AspA2I	CCTAGG	1 cut(s)	356
AspLEI	GCGC	4 cut(s)	65, 196, 456, 1623
AspS9I	GGNCC	5 cut(s)	22, 352, 637, 974, 1300
AsuC2I	CCSGG	1 cut(s)	743
AsuHPI	GGTGA	4 cut(s)	503, 584, 1018, 1397
AvaI	CYCGRG	1 cut(s)	1097
AvaII	GGWCC	4 cut(s)	22, 352, 637, 974
AvrII	CCTAGG	1 cut(s)	356
BaeGI	GKGCMC	2 cut(s)	526, 534
BaeI	ACNNNNGTAYC	2 cut(s)	1548, 1581
BanII	GRGCYC	1 cut(s)	473
Bbv12I	GWGCWC	2 cut(s)	473, 526
BbvI	GCAGC	1 cut(s)	1654
BccI	CCATC	5 cut(s)	123, 354, 1312, 1726, 1835
BceAI	ACGGC	2 cut(s)	78, 613
BciT130I	CCWGG	2 cut(s)	1145, 1383
BciVI	GTATCC	1 cut(s)	283
BclI	TGATCA	1 cut(s)	85
BcnI	CCSGG	1 cut(s)	743
BcoDI	GTCTC	2 cut(s)	176, 1098
BfaI	CTAG	3 cut(s)	357, 378, 722
BfoI	RGCGCY	2 cut(s)	457, 1624
BfrI	CTTAAG	1 cut(s)	1624
BfuI	GTATCC	1 cut(s)	283
BisI	GCNGC	2 cut(s)	245, 1643
BlnI	CCTAGG	1 cut(s)	356
BlpI	GCTNAGC	2 cut(s)	462, 626
BlsI	GCNGC	2 cut(s)	246, 1644
BmcAI	AGTACT	1 cut(s)	301
Bme1390I	CCNGG	3 cut(s)	743, 1145, 1383
Bme18I	GGWCC	4 cut(s)	22, 352, 637, 974
BmeT110I	CYCGRG	1 cut(s)	1097
BmgT120I	GGNCC	5 cut(s)	22, 352, 637, 974, 1300
BmiI	GGNNCC	5 cut(s)	6, 186, 206, 354, 1302
BmrFI	CCNGG	3 cut(s)	743, 1145, 1383
BmsI	GCATC	4 cut(s)	901, 922, 1410, 1629
BplI	GAGNNNNNCTC	2 cut(s)	455, 487
BpmI	CTGGAG	2 cut(s)	158, 457
Bpu10I	CCTNAGC	1 cut(s)	1296
Bpu1102I	GCTNAGC	2 cut(s)	462, 626
BpuEI	CTTGAG	1 cut(s)	1534
BpuMI	CCSGG	1 cut(s)	743
BsaAI	YACGTR	2 cut(s)	384, 1199
BsaBI	GATNNNNATC	1 cut(s)	1807
BsaHI	GRCGYC	1 cut(s)	169
BsaJI	CCNNGG	6 cut(s)	8, 356, 741, 970, 1144, 1758
BsaWI	WCCGGW	1 cut(s)	569
BsaXI	ACNNNNNCTCC	2 cut(s)	1737, 1767
Bsc4I	CCNNNNNNNGG	4 cut(s)	181, 748, 1486, 1841
Bse118I	RCCGGY	2 cut(s)	147, 569
Bse3DI	GCAATG	2 cut(s)	520, 1611
Bse8I	GATNNNNATC	1 cut(s)	1807
BseBI	CCWGG	2 cut(s)	1145, 1383
BseDI	CCNNGG	6 cut(s)	8, 356, 741, 970, 1144, 1758
BseGI	GGATG	5 cut(s)	502, 916, 937, 1027, 1737
BseJI	GATNNNNATC	1 cut(s)	1807
BseLI	CCNNNNNNNGG	4 cut(s)	181, 748, 1486, 1841
BseMI	GCAATG	2 cut(s)	520, 1611
BseMII	CTCAG	4 cut(s)	281, 453, 1310, 1517
BseRI	GAGGAG	1 cut(s)	112
BseSI	GKGCMC	2 cut(s)	526, 534
BseXI	GCAGC	1 cut(s)	1654
BseYI	CCCAGC	2 cut(s)	534, 1354
Bsh1236I	CGCG	4 cut(s)	65, 194, 244, 1813
Bsh1285I	CGRYCG	2 cut(s)	166, 1222
BshFI	GGCC	1 cut(s)	1301
BshTI	ACCGGT	1 cut(s)	569
BsiEI	CGRYCG	2 cut(s)	166, 1222
BsiHKAI	GWGCWC	2 cut(s)	473, 526
BsiHKCI	CYCGRG	1 cut(s)	1097
BsiSI	CCGG	3 cut(s)	148, 570, 743
BslFI	GGGAC	3 cut(s)	271, 1089, 1798
BslI	CCNNNNNNNGG	4 cut(s)	181, 748, 1486, 1841
BsmAI	GTCTC	2 cut(s)	176, 1098
BsmBI	CGTCTC	1 cut(s)	176
BsmFI	GGGAC	3 cut(s)	271, 1089, 1798
BsmI	GAATGC	1 cut(s)	1297
BsnI	GGCC	1 cut(s)	1301
BsoBI	CYCGRG	1 cut(s)	1097
Bsp1286I	GDGCHC	3 cut(s)	473, 526, 534
Bsp143I	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
Bsp1720I	GCTNAGC	2 cut(s)	462, 626
Bsp19I	CCATGG	1 cut(s)	970
Bsp68I	TCGCGA	1 cut(s)	1813
BspACI	CCGC	9 cut(s)	78, 244, 263, 339, 1115, 1131, 1226, 1705, 1856
BspANI	GGCC	1 cut(s)	1301
BspCNI	CTCAG	4 cut(s)	280, 454, 1309, 1516
BspFNI	CGCG	4 cut(s)	65, 194, 244, 1813
BspHI	TCATGA	1 cut(s)	1285
BspLI	GGNNCC	5 cut(s)	6, 186, 206, 354, 1302
BspPI	GGATC	2 cut(s)	97, 761
BspTI	CTTAAG	1 cut(s)	1624
BsrBI	CCGCTC	1 cut(s)	1226
BsrDI	GCAATG	2 cut(s)	520, 1611
BsrFI	RCCGGY	2 cut(s)	147, 569
BssAI	RCCGGY	2 cut(s)	147, 569
BssECI	CCNNGG	6 cut(s)	8, 356, 741, 970, 1144, 1758
BssMI	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
BssNI	GRCGYC	1 cut(s)	169
BssT1I	CCWWGG	2 cut(s)	356, 970
Bst2UI	CCWGG	2 cut(s)	1145, 1383
Bst4CI	ACNGT	6 cut(s)	164, 304, 329, 419, 871, 1092
Bst6I	CTCTTC	1 cut(s)	1268
BstACI	GRCGYC	1 cut(s)	169
BstAFI	CTTAAG	1 cut(s)	1624
BstBAI	YACGTR	2 cut(s)	384, 1199
BstC8I	GCNNGC	4 cut(s)	139, 153, 337, 540
BstDEI	CTNAG	5 cut(s)	267, 462, 626, 1296, 1503
BstDSI	CCRYGG	1 cut(s)	970
BstEII	GGTNACC	1 cut(s)	341
BstF5I	GGATG	5 cut(s)	502, 916, 937, 1027, 1737
BstFNI	CGCG	4 cut(s)	65, 194, 244, 1813
BstH2I	RGCGCY	2 cut(s)	457, 1624
BstHHI	GCGC	4 cut(s)	65, 196, 456, 1623
BstKTI	GATC	9 cut(s)	88, 105, 182, 217, 577, 769, 1222, 1378, 1811
BstMAI	GTCTC	2 cut(s)	176, 1098
BstMBI	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
BstMCI	CGRYCG	2 cut(s)	166, 1222
BstMWI	GCNNNNNNNGC	6 cut(s)	77, 193, 202, 824, 920, 1862
BstNI	CCWGG	2 cut(s)	1145, 1383
BstNSI	RCATGY	1 cut(s)	815
BstPI	GGTNACC	1 cut(s)	341
BstSCI	CCNGG	3 cut(s)	741, 1143, 1381
BstSLI	GKGCMC	2 cut(s)	526, 534
BstSNI	TACGTA	1 cut(s)	1199
BstUI	CGCG	4 cut(s)	65, 194, 244, 1813
BstV1I	GCAGC	1 cut(s)	1654
BstXI	CCANNNNNNTGG	1 cut(s)	1658
BsuI	GTATCC	1 cut(s)	283
BsuRI	GGCC	1 cut(s)	1301
BtgI	CCRYGG	1 cut(s)	970
BtgZI	GCGATG	1 cut(s)	1333
BtsCI	GGATG	5 cut(s)	502, 916, 937, 1027, 1737
BtsI	GCAGTG	1 cut(s)	484
BtsIMutI	CAGTG	2 cut(s)	484, 1088
BtuMI	TCGCGA	1 cut(s)	1813
Cac8I	GCNNGC	4 cut(s)	139, 153, 337, 540
CaiI	CAGNNNCTG	1 cut(s)	1013
CciI	TCATGA	1 cut(s)	1285
CfoI	GCGC	4 cut(s)	65, 196, 456, 1623
Cfr10I	RCCGGY	2 cut(s)	147, 569
Cfr13I	GGNCC	5 cut(s)	22, 352, 637, 974, 1300
CseI	GACGC	4 cut(s)	143, 158, 250, 619
Csp6I	GTAC	5 cut(s)	232, 300, 385, 1196, 1565
CspAI	ACCGGT	1 cut(s)	569
CspCI	CAANNNNNGTGG	2 cut(s)	40, 75
CviQI	GTAC	5 cut(s)	232, 300, 385, 1196, 1565
DdeI	CTNAG	5 cut(s)	267, 462, 626, 1296, 1503
DpnI	GATC	9 cut(s)	87, 104, 181, 216, 576, 768, 1221, 1377, 1810
DpnII	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
DraI	TTTAAA	1 cut(s)	1281
Eam1104I	CTCTTC	1 cut(s)	1268
EarI	CTCTTC	1 cut(s)	1268
Ecl136II	GAGCTC	1 cut(s)	471
Eco105I	TACGTA	1 cut(s)	1199
Eco130I	CCWWGG	2 cut(s)	356, 970
Eco24I	GRGCYC	1 cut(s)	473
Eco32I	GATATC	1 cut(s)	997
Eco47I	GGWCC	4 cut(s)	22, 352, 637, 974
Eco53kI	GAGCTC	1 cut(s)	471
Eco57I	CTGAAG	2 cut(s)	821, 1862
Eco88I	CYCGRG	1 cut(s)	1097
Eco91I	GGTNACC	1 cut(s)	341
EcoICRI	GAGCTC	1 cut(s)	471
EcoO109I	RGGNCCY	2 cut(s)	352, 1300
EcoO65I	GGTNACC	1 cut(s)	341
EcoRI	GAATTC	1 cut(s)	1188
EcoRII	CCWGG	2 cut(s)	1143, 1381
EcoRV	GATATC	1 cut(s)	997
EcoT14I	CCWWGG	2 cut(s)	356, 970
EcoT22I	ATGCAT	1 cut(s)	898
EcoT38I	GRGCYC	1 cut(s)	473
ErhI	CCWWGG	2 cut(s)	356, 970
Esp3I	CGTCTC	1 cut(s)	176
FalI	AAGNNNNNCTT	2 cut(s)	1226, 1258
FaqI	GGGAC	3 cut(s)	271, 1089, 1798
FauI	CCCGC	1 cut(s)	1124
FauNDI	CATATG	1 cut(s)	892
FbaI	TGATCA	1 cut(s)	85
Fnu4HI	GCNGC	2 cut(s)	245, 1643
FokI	GGATG	5 cut(s)	509, 923, 944, 1034, 1744
FriOI	GRGCYC	1 cut(s)	473
Fsp4HI	GCNGC	2 cut(s)	245, 1643
FspBI	CTAG	3 cut(s)	357, 378, 722
GlaI	GCGC	4 cut(s)	64, 195, 455, 1622
GluI	GCNGC	2 cut(s)	245, 1643
GsaI	CCCAGC	2 cut(s)	538, 1358
GsuI	CTGGAG	2 cut(s)	158, 457
HaeII	RGCGCY	2 cut(s)	457, 1624
HaeIII	GGCC	1 cut(s)	1301
HapII	CCGG	3 cut(s)	148, 570, 743
HgaI	GACGC	4 cut(s)	143, 158, 250, 619
HhaI	GCGC	4 cut(s)	65, 196, 456, 1623
Hin1I	GRCGYC	1 cut(s)	169
Hin6I	GCGC	4 cut(s)	63, 194, 454, 1621
HinP1I	GCGC	4 cut(s)	63, 194, 454, 1621
HinfI	GANTC	4 cut(s)	668, 1166, 1469, 1574
HpaII	CCGG	3 cut(s)	148, 570, 743
HphI	GGTGA	4 cut(s)	503, 584, 1018, 1397
Hpy166II	GTNNAC	5 cut(s)	524, 756, 1388, 1510, 1557
Hpy188I	TCNGA	9 cut(s)	12, 99, 184, 270, 282, 325, 766, 1264, 1638
Hpy188III	TCNNGA	8 cut(s)	175, 350, 977, 1097, 1286, 1343, 1533, 1812
Hpy8I	GTNNAC	5 cut(s)	524, 756, 1388, 1510, 1557
Hpy99I	CGWCG	6 cut(s)	134, 137, 164, 244, 599, 1319
HpyAV	CCTTC	8 cut(s)	38, 116, 166, 796, 838, 937, 1011, 1117
HpyCH4III	ACNGT	6 cut(s)	164, 304, 329, 419, 871, 1092
HpyCH4IV	ACGT	3 cut(s)	383, 589, 1198
HpyCH4V	TGCA	8 cut(s)	414, 441, 524, 896, 923, 1466, 1642, 1865
HpyF10VI	GCNNNNNNNGC	6 cut(s)	77, 193, 202, 824, 920, 1862
HpyF3I	CTNAG	5 cut(s)	267, 462, 626, 1296, 1503
HpySE526I	ACGT	3 cut(s)	383, 589, 1198
Hsp92I	GRCGYC	1 cut(s)	169
HspAI	GCGC	4 cut(s)	63, 194, 454, 1621
Ksp22I	TGATCA	1 cut(s)	85
Kzo9I	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
LmnI	GCTCC	6 cut(s)	10, 184, 210, 476, 1345, 1717
Lsp1109I	GCAGC	1 cut(s)	1654
LweI	GCATC	4 cut(s)	901, 922, 1410, 1629
MaeI	CTAG	3 cut(s)	357, 378, 722
MaeII	ACGT	3 cut(s)	383, 589, 1198
MaeIII	GTNAC	4 cut(s)	304, 341, 1024, 1210
MalI	GATC	9 cut(s)	87, 104, 181, 216, 576, 768, 1221, 1377, 1810
MbiI	CCGCTC	1 cut(s)	1226
MboI	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
MboII	GAAGA	3 cut(s)	1285, 1299, 1370
MfeI	CAATTG	2 cut(s)	409, 1255
MhlI	GDGCHC	3 cut(s)	473, 526, 534
MlyI	GAGTC	1 cut(s)	1478
MmeI	TCCRAC	1 cut(s)	1723
Mph1103I	ATGCAT	1 cut(s)	898
MseI	TTAA	7 cut(s)	732, 836, 927, 1280, 1551, 1625, 1887
MslI	CAYNNNNRTG	2 cut(s)	311, 1290
MspA1I	CMGCKG	2 cut(s)	1358, 1672
MspCI	CTTAAG	1 cut(s)	1624
MspI	CCGG	3 cut(s)	148, 570, 743
MspR9I	CCNGG	3 cut(s)	743, 1145, 1383
MunI	CAATTG	2 cut(s)	409, 1255
Mva1269I	GAATGC	1 cut(s)	1297
MvaI	CCWGG	2 cut(s)	1145, 1383
MvnI	CGCG	4 cut(s)	65, 194, 244, 1813
MwoI	GCNNNNNNNGC	6 cut(s)	77, 193, 202, 824, 920, 1862
NciI	CCSGG	1 cut(s)	743
NcoI	CCATGG	1 cut(s)	970
NdeI	CATATG	1 cut(s)	892
NdeII	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
NlaIV	GGNNCC	5 cut(s)	6, 186, 206, 354, 1302
NmuCI	GTSAC	3 cut(s)	304, 1024, 1210
NruI	TCGCGA	1 cut(s)	1813
NsiI	ATGCAT	1 cut(s)	898
NspI	RCATGY	1 cut(s)	815
OliI	CACNNNNGTG	1 cut(s)	311
PagI	TCATGA	1 cut(s)	1285
PciI	ACATGT	1 cut(s)	811
PctI	GAATGC	1 cut(s)	1297
PfeI	GAWTC	3 cut(s)	668, 1166, 1574
PflMI	CCANNNNNTGG	1 cut(s)	1486
PinAI	ACCGGT	1 cut(s)	569
PkrI	GCNGC	2 cut(s)	246, 1644
Ple19I	CGATCG	1 cut(s)	1222
PleI	GAGTC	1 cut(s)	1477
PpsI	GAGTC	1 cut(s)	1477
Ppu21I	YACGTR	2 cut(s)	384, 1199
PpuMI	RGGWCCY	1 cut(s)	352
PscI	ACATGT	1 cut(s)	811
PsiI	TTATAA	1 cut(s)	990
Psp124BI	GAGCTC	1 cut(s)	473
Psp5II	RGGWCCY	1 cut(s)	352
Psp6I	CCWGG	2 cut(s)	1143, 1381
PspEI	GGTNACC	1 cut(s)	341
PspFI	CCCAGC	2 cut(s)	534, 1354
PspGI	CCWGG	2 cut(s)	1143, 1381
PspN4I	GGNNCC	5 cut(s)	6, 186, 206, 354, 1302
PspPI	GGNCC	5 cut(s)	22, 352, 637, 974, 1300
PspPPI	RGGWCCY	1 cut(s)	352
PstNI	CAGNNNCTG	1 cut(s)	1013
PvuI	CGATCG	1 cut(s)	1222
PvuII	CAGCTG	2 cut(s)	1358, 1672
RruI	TCGCGA	1 cut(s)	1813
RsaI	GTAC	5 cut(s)	233, 301, 386, 1197, 1566
RsaNI	GTAC	5 cut(s)	232, 300, 385, 1196, 1565
RseI	CAYNNNNRTG	2 cut(s)	311, 1290
SacI	GAGCTC	1 cut(s)	473
SaqAI	TTAA	7 cut(s)	732, 836, 927, 1280, 1551, 1625, 1887
SatI	GCNGC	2 cut(s)	245, 1643
Sau3AI	GATC	9 cut(s)	85, 102, 179, 214, 574, 766, 1219, 1375, 1808
Sau96I	GGNCC	5 cut(s)	22, 352, 637, 974, 1300
ScaI	AGTACT	1 cut(s)	301
SchI	GAGTC	1 cut(s)	1478
ScrFI	CCNGG	3 cut(s)	743, 1145, 1383
SduI	GDGCHC	3 cut(s)	473, 526, 534
SfaNI	GCATC	4 cut(s)	901, 922, 1410, 1629
SgrAI	CRCCGGYG	1 cut(s)	147
SinI	GGWCC	4 cut(s)	22, 352, 637, 974
SmiMI	CAYNNNNRTG	2 cut(s)	311, 1290
SmlI	CTYRAG	2 cut(s)	1513, 1624
SmoI	CTYRAG	2 cut(s)	1513, 1624
SnaBI	TACGTA	1 cut(s)	1199
SsiI	CCGC	9 cut(s)	78, 244, 263, 339, 1115, 1131, 1226, 1705, 1856
SspI	AATATT	2 cut(s)	562, 793
SspMI	CTAG	3 cut(s)	357, 378, 722
SstI	GAGCTC	1 cut(s)	473
StyD4I	CCNGG	3 cut(s)	741, 1143, 1381
StyI	CCWWGG	2 cut(s)	356, 970
TaaI	ACNGT	6 cut(s)	164, 304, 329, 419, 871, 1092
TaiI	ACGT	3 cut(s)	386, 592, 1201
TaqI	TCGA	4 cut(s)	159, 1222, 1413, 1494
TatI	WGTACW	1 cut(s)	299
TauI	GCSGC	1 cut(s)	247
TfiI	GAWTC	3 cut(s)	668, 1166, 1574
Tru1I	TTAA	7 cut(s)	732, 836, 927, 1280, 1551, 1625, 1887
Tru9I	TTAA	7 cut(s)	732, 836, 927, 1280, 1551, 1625, 1887
TscAI	CASTG	2 cut(s)	484, 1095
TseFI	GTSAC	3 cut(s)	304, 1024, 1210
TseI	GCWGC	1 cut(s)	1642
Tsp45I	GTSAC	3 cut(s)	304, 1024, 1210
TspDTI	ATGAA	7 cut(s)	660, 666, 870, 956, 1457, 1587, 1622
TspGWI	ACGGA	1 cut(s)	31
TspRI	CASTG	2 cut(s)	484, 1095
Van91I	CCANNNNNTGG	1 cut(s)	1486
Vha464I	CTTAAG	1 cut(s)	1624
VneI	GTGCAC	1 cut(s)	522
VpaK11BI	GGWCC	4 cut(s)	22, 352, 637, 974
XapI	RAATTY	3 cut(s)	1149, 1188, 1655
XceI	RCATGY	1 cut(s)	815
XmaJI	CCTAGG	1 cut(s)	356
XspI	CTAG	3 cut(s)	357, 378, 722
ZrmI	AGTACT	1 cut(s)	301
Zsp2I	ATGCAT	1 cut(s)	898

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.