Rroxscaffold_7G00159650

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009104
Physical Location & Seq
Reverse (-)
2775808 .. 2783825
8018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159650.1

Sequence Viewer

Length: 1560 bp
ATGGAAAAAGGGTGCTTGCTTGGCGTGAAAGGAACTTGCGACGTATGTAGACAGCTAGATATGTACAACTATGAGCGGAATTTAGGAGGGAGCATCTCCCGTGCTCTCCAGCAGATGAGCAATGCCACCGTGATCGACGAGAAGGTCCTGAACGAGTGCCTCAACGAGATCACACGCGCTCTTCTCCAATCCGATGTGCAATTCAAGCTCGTCCGCGATATGCAGACCAATATCAAGAAGATCGTCAATTTGGAGGATCTCGCTGCCGGTCACAACAAGCGTAGGATCATCCAGAATGCTATTTTTAATGAGCTTTGCAAAATGCTTGATCCGGGGAAACCCTCTTTCACTCCGAAGAAAGGAAAACCGAGTGTGGTCATGTTTGTTGGTTTGCAAGGGTCTGGAAAGACTACAACTTGCACAAAATATGCATATTATCATCAGAAGAAAGGTTGGAAACCTGCCCTGGTGTGCGCTGATACTTTCCGAGCTGGTGCATTTGATCAGTTGAAGCAGAATGCTACCAAGGCAAAGATTCCGTTTTATGGAAGCTATATGGAGTCTGACCCTGCAAAAATTGCGGTGGAAGGAGTGGAGACTTTTAAGAAGGAGAATTGCGATCTTATAATTGTTGATACCAGTGGGCGTCACAAACAGGAAGCTGCTCTTTTTGAAGAGATGCGTCAGGTAGCTGAAGCAACGAAACCTGATCTTGTTATATTTGTTATGGATAGCAGTATTGGTCAGGCCGCGTTTGATCAAGCTCAAGCATTTAAGCAAAGTGCTGCAGTTGGAGCTGTTATTGTTACCAAAATGGATGGCCATGCAAAGGGAGGTGGTGCTCTTAGTGCTGTTGCAGCAACAAAGAGTCCTGTTATATTTATTGGAACAGGAGAGCATATGGATGAGTTTGAAGTGTTTGATGTTAAGTCGTTTGTCAGCCGTCTATTAGGCATGGGTGACTGGTCTGGATTTATGGACAAGATTCAGGAAGTTGTTCCTAAACTTGACAACCAGCCAGAGCTTCTGCAAAAGCTTTCAGAAGGGACCTTCACGTTGAGGATTATGTATGAGCAGTTTCAGAACTTGCTTCAAATGGGTCCACTTAGCCAGCTTACTTCAATGCTTCCAGGTGCTGAGTTAATGCCGAAAGGTAATGATAAGGAAAGCCAGGCCAAAATCAAGCGGTACATGACCATGATGGACTCAATGACAAATGAAGAGTTGGACAGCACAAATACAAAAATCATTACTGAGTCTCGCATGGTGCGAATAGCACGAGGTTCTGGTCATCTAGTAAGAGAAGTGATGGAGATGTTTGAAGAGTACAAGCGCCTTGCTAAAATATGGAGCAAAATGAAAGGAATTAAGATTCCTAAGAAGGGTGGTATCAGTCCACTAAACCAAAACTTGAATGCACAGCAAATGAGCAAAGTCCTACCTCCCCAGATGTTGAAGCAGATTGGTGGCATGGGTGGGTTACAGAACTTGATGAAGCAAATGGGTTCAATGGGTTCTGCTAAAGAAATGATGGGAATGCTCGGAGGCGGGGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

519

Amino Acids

57.44

Weight (kDa)

9.03

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 28 - 105 3.6e-14 SRP54-type protein, helical bundle domain
SRP54 PF00448 123 - 318 6.6e-78 SRP54-type protein, GTPase domain
cobW PF02492 125 - 275 7.9e-06 CobW/HypB/UreG, nucleotide-binding domain
SRP_SPB PF02978 350 - 449 8.6e-27 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
AasI GACNNNNNNGTC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 469
AccBSI CCGCTC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 49
AccII CGCG 3 cut(s) 177, 216, 752
AciI CCGC 6 cut(s) 76, 214, 581, 750, 1186, 1548
AclWI GGATC 3 cut(s) 264, 293, 323
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 714
AcyI GRCGYC 1 cut(s) 646
AfaI GTAC 3 cut(s) 65, 1190, 1328
AfiI CCNNNNNNNGG 4 cut(s) 359, 545, 829, 1382
AjnI CCWGG 3 cut(s) 465, 1129, 1170
Alw21I GWGCWC 2 cut(s) 106, 844
Alw26I GTCTC 2 cut(s) 590, 1263
AlwI GGATC 3 cut(s) 264, 293, 323
AlwNI CAGNNNCTG 1 cut(s) 1136
AoxI GGCC 3 cut(s) 747, 820, 1173
ApeKI GCWGC 4 cut(s) 263, 662, 785, 857
ApoI RAATTY 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 3 cut(s) 179, 476, 1335
AspS9I GGNCC 3 cut(s) 145, 1047, 1100
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 971
AvaII GGWCC 3 cut(s) 145, 1047, 1100
BalI TGGCCA 1 cut(s) 822
BauI CACGAG 1 cut(s) 1278
Bbv12I GWGCWC 2 cut(s) 106, 844
BbvI GCAGC 4 cut(s) 250, 649, 772, 869
BccI CCATC 4 cut(s) 812, 1195, 1303, 1525
BceAI ACGGC 1 cut(s) 927
BciT130I CCWGG 3 cut(s) 467, 1131, 1172
BclI TGATCA 2 cut(s) 502, 757
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 590, 1263
BfaI CTAG 2 cut(s) 56, 1295
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 1336
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 5 cut(s) 264, 663, 750, 786, 858
BlsI GCNGC 5 cut(s) 265, 664, 751, 787, 859
Bme1390I CCNGG 4 cut(s) 333, 467, 1131, 1172
Bme18I GGWCC 3 cut(s) 145, 1047, 1100
BmgT120I GGNCC 3 cut(s) 145, 1047, 1100
BmiI GGNNCC 2 cut(s) 1048, 1101
BmrFI CCNGG 4 cut(s) 333, 467, 1131, 1172
BmsI GCATC 2 cut(s) 102, 669
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 750
BpuMI CCSGG 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 646
BsaJI CCNNGG 3 cut(s) 332, 465, 525
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 545, 829, 1382
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 639, 968
Bse3DI GCAATG 1 cut(s) 127
BseBI CCWGG 3 cut(s) 467, 1131, 1172
BseDI CCNNGG 3 cut(s) 332, 465, 525
BseGI GGATG 3 cut(s) 288, 823, 910
BseLI CCNNNNNNNGG 4 cut(s) 359, 545, 829, 1382
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 1128, 1245
BseNI ACTGG 2 cut(s) 639, 968
BseXI GCAGC 4 cut(s) 250, 649, 772, 869
Bsh1236I CGCG 3 cut(s) 177, 216, 752
BshFI GGCC 3 cut(s) 749, 822, 1175
BsiHKAI GWGCWC 2 cut(s) 106, 844
BsiSI CCGG 2 cut(s) 267, 332
BslFI GGGAC 1 cut(s) 1060
BslI CCNNNNNNNGG 4 cut(s) 359, 545, 829, 1382
BsmAI GTCTC 2 cut(s) 590, 1263
BsmFI GGGAC 1 cut(s) 1060
BsmI GAATGC 4 cut(s) 301, 523, 1420, 1542
BsnI GGCC 3 cut(s) 749, 822, 1175
Bsp1286I GDGCHC 2 cut(s) 106, 844
Bsp1407I TGTACA 1 cut(s) 63
BspACI CCGC 6 cut(s) 76, 214, 581, 750, 1186, 1548
BspANI GGCC 3 cut(s) 749, 822, 1175
BspCNI CTCAG 2 cut(s) 1129, 1246
BspFNI CGCG 3 cut(s) 177, 216, 752
BspLI GGNNCC 2 cut(s) 1048, 1101
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 469
BspPI GGATC 3 cut(s) 264, 293, 323
BspQI GCTCTTC 1 cut(s) 186
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 127
BsrFI RCCGGY 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 2 cut(s) 639, 968
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 332, 465, 525
BssNI GRCGYC 1 cut(s) 646
BssSI CACGAG 1 cut(s) 1278
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 1278
Bst2UI CCWGG 3 cut(s) 467, 1131, 1172
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 4 cut(s) 186, 669, 1215, 1317
BstACI GRCGYC 1 cut(s) 646
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 17, 1112
BstDEI CTNAG 5 cut(s) 845, 1106, 1137, 1254, 1377
BstF5I GGATG 3 cut(s) 288, 823, 910
BstFNI CGCG 3 cut(s) 177, 216, 752
BstH2I RGCGCY 1 cut(s) 1336
BstHHI GCGC 3 cut(s) 179, 476, 1335
BstMAI GTCTC 2 cut(s) 590, 1263
BstMWI GCNNNNNNNGC 7 cut(s) 21, 205, 527, 578, 794, 848, 857
BstNI CCWGG 3 cut(s) 467, 1131, 1172
BstSCI CCNGG 4 cut(s) 331, 465, 1129, 1170
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 3 cut(s) 177, 216, 752
BstV1I GCAGC 4 cut(s) 250, 649, 772, 869
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 3 cut(s) 749, 822, 1175
BtsCI GGATG 3 cut(s) 288, 823, 910
BtsIMutI CAGTG 1 cut(s) 646
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 2 cut(s) 17, 1112
CaiI CAGNNNCTG 1 cut(s) 1136
CfoI GCGC 3 cut(s) 179, 476, 1335
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 3 cut(s) 145, 1047, 1100
CseI GACGC 2 cut(s) 635, 671
Csp6I GTAC 3 cut(s) 64, 1189, 1327
CviAII CATG 7 cut(s) 379, 824, 955, 1192, 1198, 1264, 1471
CviQI GTAC 3 cut(s) 64, 1189, 1327
DdeI CTNAG 5 cut(s) 845, 1106, 1137, 1254, 1377
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 4 cut(s) 186, 669, 1215, 1317
EarI CTCTTC 4 cut(s) 186, 669, 1215, 1317
Eco130I CCWWGG 1 cut(s) 525
Eco47I GGWCC 3 cut(s) 145, 1047, 1100
Eco57I CTGAAG 1 cut(s) 714
EcoO109I RGGNCCY 2 cut(s) 145, 1047
EcoRII CCWGG 3 cut(s) 465, 1129, 1170
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 7 cut(s) 382, 827, 958, 1195, 1201, 1267, 1474
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 1 cut(s) 1060
FatI CATG 7 cut(s) 378, 823, 954, 1191, 1197, 1263, 1470
FauI CCCGC 1 cut(s) 1541
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 2 cut(s) 502, 757
FblI GTMKAC 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 264, 663, 750, 786, 858
FokI GGATG 3 cut(s) 275, 830, 917
Fsp4HI GCNGC 5 cut(s) 264, 663, 750, 786, 858
FspBI CTAG 2 cut(s) 56, 1295
GlaI GCGC 3 cut(s) 178, 475, 1334
GluI GCNGC 5 cut(s) 264, 663, 750, 786, 858
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 1336
HaeIII GGCC 3 cut(s) 749, 822, 1175
HapII CCGG 2 cut(s) 267, 332
HgaI GACGC 2 cut(s) 635, 671
HhaI GCGC 3 cut(s) 179, 476, 1335
Hin1I GRCGYC 1 cut(s) 646
Hin1II CATG 7 cut(s) 382, 827, 958, 1195, 1201, 1267, 1474
Hin6I GCGC 3 cut(s) 177, 474, 1333
HinP1I GCGC 3 cut(s) 177, 474, 1333
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 7 cut(s) 535, 560, 868, 985, 1205, 1256, 1372
HpaII CCGG 2 cut(s) 267, 332
HphI GGTGA 1 cut(s) 971
Hpy166II GTNNAC 3 cut(s) 50, 1103, 1397
Hpy188I TCNGA 8 cut(s) 193, 354, 444, 488, 565, 1042, 1083, 1544
Hpy188III TCNNGA 6 cut(s) 148, 235, 292, 402, 969, 989
Hpy8I GTNNAC 3 cut(s) 50, 1103, 1397
Hpy99I CGWCG 2 cut(s) 44, 140
HpyAV CCTTC 6 cut(s) 136, 581, 601, 1037, 1060, 1375
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 42, 1055
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 205, 527, 578, 794, 848, 857
HpyF3I CTNAG 5 cut(s) 845, 1106, 1137, 1254, 1377
HpySE526I ACGT 2 cut(s) 42, 1055
Hsp92I GRCGYC 1 cut(s) 646
Hsp92II CATG 7 cut(s) 382, 827, 958, 1195, 1201, 1267, 1474
HspAI GCGC 3 cut(s) 177, 474, 1333
Ksp22I TGATCA 2 cut(s) 502, 757
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 3 cut(s) 90, 794, 1350
Lsp1109I GCAGC 4 cut(s) 250, 649, 772, 869
LweI GCATC 2 cut(s) 102, 669
MaeI CTAG 2 cut(s) 56, 1295
MaeII ACGT 2 cut(s) 42, 1055
MaeIII GTNAC 5 cut(s) 269, 647, 805, 959, 1479
MbiI CCGCTC 1 cut(s) 76
MboII GAAGA 7 cut(s) 173, 250, 367, 457, 686, 1232, 1334
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 106, 844
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 7 cut(s) 79, 200, 247, 576, 613, 627, 1365
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 4 cut(s) 569, 877, 1199, 1265
MmeI TCCRAC 3 cut(s) 434, 772, 1206
MnlI CCTC 9 cut(s) 80, 170, 247, 352, 827, 1053, 1274, 1452, 1538
Mox20I TGGCCA 1 cut(s) 822
Mph1103I ATGCAT 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 996
MscI TGGCCA 1 cut(s) 822
MseI TTAA 6 cut(s) 306, 603, 774, 927, 1142, 1368
MslI CAYNNNNRTG 2 cut(s) 903, 1196
Msp20I TGGCCA 1 cut(s) 822
MspI CCGG 2 cut(s) 267, 332
MspR9I CCNGG 4 cut(s) 333, 467, 1131, 1172
Mva1269I GAATGC 4 cut(s) 301, 523, 1420, 1542
MvaI CCWGG 3 cut(s) 467, 1131, 1172
MvnI CGCG 3 cut(s) 177, 216, 752
MwoI GCNNNNNNNGC 7 cut(s) 21, 205, 527, 578, 794, 848, 857
NciI CCSGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 900
NlaIII CATG 7 cut(s) 382, 827, 958, 1195, 1201, 1267, 1474
NlaIV GGNNCC 2 cut(s) 1048, 1101
NmuCI GTSAC 3 cut(s) 269, 647, 959
NsiI ATGCAT 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 186
PcsI WCGNNNNNNNCGW 1 cut(s) 1267
PctI GAATGC 4 cut(s) 301, 523, 1420, 1542
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 3 cut(s) 535, 985, 1372
PkrI GCNGC 5 cut(s) 265, 664, 751, 787, 859
PleI GAGTC 4 cut(s) 568, 876, 1199, 1264
PpsI GAGTC 4 cut(s) 568, 876, 1199, 1264
PpuMI RGGWCCY 2 cut(s) 145, 1047
PsiI TTATAA 1 cut(s) 626
Psp5II RGGWCCY 2 cut(s) 145, 1047
Psp6I CCWGG 3 cut(s) 465, 1129, 1170
PspGI CCWGG 3 cut(s) 465, 1129, 1170
PspN4I GGNNCC 2 cut(s) 1048, 1101
PspPI GGNCC 3 cut(s) 145, 1047, 1100
PspPPI RGGWCCY 2 cut(s) 145, 1047
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 1136
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 3 cut(s) 65, 1190, 1328
RsaNI GTAC 3 cut(s) 64, 1189, 1327
RseI CAYNNNNRTG 2 cut(s) 903, 1196
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 6 cut(s) 306, 603, 774, 927, 1142, 1368
SatI GCNGC 5 cut(s) 264, 663, 750, 786, 858
Sau96I GGNCC 3 cut(s) 145, 1047, 1100
SchI GAGTC 4 cut(s) 569, 877, 1199, 1265
ScrFI CCNGG 4 cut(s) 333, 467, 1131, 1172
SduI GDGCHC 2 cut(s) 106, 844
SfaNI GCATC 2 cut(s) 102, 669
SfcI CTRYAG 1 cut(s) 786
SinI GGWCC 3 cut(s) 145, 1047, 1100
SmiMI CAYNNNNRTG 2 cut(s) 903, 1196
SmlI CTYRAG 1 cut(s) 765
SmoI CTYRAG 1 cut(s) 765
Sse9I AATT 7 cut(s) 79, 200, 247, 576, 613, 627, 1365
SsiI CCGC 6 cut(s) 76, 214, 581, 750, 1186, 1548
SspMI CTAG 2 cut(s) 56, 1295
StyD4I CCNGG 4 cut(s) 331, 465, 1129, 1170
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 45, 1058
TaqI TCGA 1 cut(s) 135
TasI AATT 7 cut(s) 79, 200, 247, 576, 613, 627, 1365
TatI WGTACW 2 cut(s) 63, 1326
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 3 cut(s) 535, 985, 1372
Tru1I TTAA 6 cut(s) 306, 603, 774, 927, 1142, 1368
Tru9I TTAA 6 cut(s) 306, 603, 774, 927, 1142, 1368
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 3 cut(s) 269, 647, 959
TseI GCWGC 4 cut(s) 263, 662, 785, 857
Tsp45I GTSAC 3 cut(s) 269, 647, 959
TspDTI ATGAA 3 cut(s) 1233, 1373, 1508
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 1 cut(s) 646
VpaK11BI GGWCC 3 cut(s) 145, 1047, 1100
XapI RAATTY 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 2 cut(s) 56, 1295
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.