Rw6G042010

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009104
Physical Location & Seq
Forward (+)
65633518 .. 65635267
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042010.1

Sequence Viewer

Length: 1425 bp
ATGGTGTTAGCGGAATTAGGAGGGAGCATCTCCCGTGCTCTCCAGCAGATGAGCAACGCCACCGTGATCGACGAGAAGGTCCTGAACGAGTGCCTCAACGAGATCACACGCGCTCTTCTCCAATCCGATGTGCAATTCAAGCTCGTCCGCGATATGCAGACCAATATCAAGAAGATCCGTAGGATCATCCAGAATGCTATTTTTAATGAGCTCTGCAAAATGCTTGATCCAGGCAAACCCTCTTTCACTCCGAAGAAAGGAAAACCGAGTGTGGTCATGTTTGTTGGTTTGCAAGGGTCTGGAAAGACTACAACTTGCACAAAATATGCATATTATCATCAGAAGAAAGGTTGGAAACCTGCCCTGGTGTGCGCTGATACTTTCCGAGCTGGTGCATTTGATCAGTTGAAGCAGAATGCTACCAAGGCAAAGATTCCGTTTTATGGAAGCTATATGGAGTCGGACCCTGTGAAAATTGCGGTGGAAGGAGTGGAGACTTTTAAGAAGGAGAATTGCGATCTTATAATTGTTGATACCAGTGGGCGTCACAAACAGGAAGCTGCTCTTTTTGAAGAGATGCGTCAGGTGGCTGAAGCGACGAAACCAGACCTTGCCGCGTTTGATCAAGCTCAAGCATTTAAGCAAAGTGCTGCAGTTGGAGCTGTTATTGTTACCAAAATGGATGGTCATGCAAAGGGAGGTGGTGCTCTTAGTGCTGTTGCAGCAACAAAGAGTCCTGTTATATTTATTGGAACAGGAGAGCATATGGATGAGTTTGAAGGGTTTGATGTTAAGGCCTTTGTCAGCCGTCTATTAGGCATGGGTGACTGGTCTGGATTTATGGACAAGATTCAGGAAGTTGTTCCTAAACTTGACAACCAGCCAGAGCTTCTGCAAAAGCTTTCAGAAGGGACCTTCACGCTGAGGATTATGTATGAGCAGTTTCAGAACTTGCTTCAAATGGGCCCACTTAGCCAGCTGACTTCAATGCTTCCAGGTGCTGAGCTAATGCCAAAAGGTAATGATAAGGAAAGCCAGGCCAAAATCAAGCGGTACATGACCATGATGGACTCGATGACAAATGAAGAGTTGGACAGCACAAATACAAAAATCATTACTGAGTCTCGTATGGTGCGAATAGCACGAGGTTCTGGTCGTCTGGTAAGAGAAGTGATGGAGATGTTTGAAGAGTACAAGCGCCTTGCTAAAATATGGAGCAAAATGAAAGGAATTAAGATTCCTAAGAAGGGTGGTATCAGTCCGCTAAACCAAAACTTTAATGCACAGCAAATGAGCAAAGTCCTACCTCCCCAGATGTTGAAGCAGATTGGTGGCATGGGTGGCTTACAGAACTTGATGAAGCAAATGGGTTCAATGGGTTCAGCTAAAGAAATGATGGGAATGCTTGGAGGCGGGGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

474

Amino Acids

52.44

Weight (kDa)

9.27

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 6 - 58 9.5e-12 SRP54-type protein, helical bundle domain
SRP54 PF00448 89 - 273 1.1e-65 SRP54-type protein, GTPase domain
SRP_SPB PF02978 305 - 404 7.3e-27 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 367
AccII CGCG 3 cut(s) 111, 150, 617
AciI CCGC 7 cut(s) 11, 148, 479, 615, 1051, 1262, 1413
AclWI GGATC 3 cut(s) 169, 191, 221
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 544
AfaI GTAC 2 cut(s) 1055, 1193
AfiI CCNNNNNNNGG 3 cut(s) 257, 443, 1247
AgsI TTSAA 9 cut(s) 139, 409, 572, 779, 959, 987, 1187, 1321, 1374
AjnI CCWGG 4 cut(s) 229, 363, 994, 1035
Alw21I GWGCWC 3 cut(s) 40, 213, 709
Alw26I GTCTC 2 cut(s) 488, 1128
AlwI GGATC 3 cut(s) 169, 191, 221
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 3 cut(s) 795, 964, 1038
ApaI GGGCCC 1 cut(s) 968
ApeKI GCWGC 3 cut(s) 560, 650, 722
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 3 cut(s) 113, 374, 1200
AspS9I GGNCC 5 cut(s) 79, 463, 912, 964, 965
AsuHPI GGTGA 1 cut(s) 836
AvaII GGWCC 3 cut(s) 79, 463, 912
BaeGI GKGCMC 1 cut(s) 968
BanII GRGCYC 2 cut(s) 213, 968
BauI CACGAG 1 cut(s) 1143
Bbv12I GWGCWC 3 cut(s) 40, 213, 709
BbvCI CCTCAGC 1 cut(s) 923
BbvI GCAGC 3 cut(s) 547, 637, 734
BccI CCATC 4 cut(s) 677, 1060, 1168, 1390
BceAI ACGGC 1 cut(s) 792
BciT130I CCWGG 4 cut(s) 231, 365, 996, 1037
BclI TGATCA 2 cut(s) 400, 622
BcoDI GTCTC 2 cut(s) 488, 1128
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 1201
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 4 cut(s) 561, 615, 651, 723
BlpI GCTNAGC 1 cut(s) 1002
BlsI GCNGC 4 cut(s) 562, 616, 652, 724
Bme1390I CCNGG 4 cut(s) 231, 365, 996, 1037
Bme18I GGWCC 3 cut(s) 79, 463, 912
BmgT120I GGNCC 5 cut(s) 79, 463, 912, 964, 965
BmiI GGNNCC 3 cut(s) 465, 913, 966
BmrFI CCNGG 4 cut(s) 231, 365, 996, 1037
BmsI GCATC 2 cut(s) 36, 567
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 923
Bpu1102I GCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 363, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 443, 1247
Bse1I ACTGG 2 cut(s) 537, 833
BseBI CCWGG 4 cut(s) 231, 365, 996, 1037
BseDI CCNNGG 2 cut(s) 363, 423
BseGI GGATG 3 cut(s) 186, 688, 775
BseLI CCNNNNNNNGG 3 cut(s) 257, 443, 1247
BseMII CTCAG 3 cut(s) 914, 993, 1110
BseNI ACTGG 2 cut(s) 537, 833
BseSI GKGCMC 1 cut(s) 968
BseXI GCAGC 3 cut(s) 547, 637, 734
Bsh1236I CGCG 3 cut(s) 111, 150, 617
BshFI GGCC 3 cut(s) 797, 966, 1040
BsiHKAI GWGCWC 3 cut(s) 40, 213, 709
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 3 cut(s) 257, 443, 1247
BsmAI GTCTC 2 cut(s) 488, 1128
BsmFI GGGAC 1 cut(s) 925
BsmI GAATGC 3 cut(s) 199, 421, 1407
BsnI GGCC 3 cut(s) 797, 966, 1040
Bsp120I GGGCCC 1 cut(s) 964
Bsp1286I GDGCHC 4 cut(s) 40, 213, 709, 968
Bsp143I GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
Bsp1720I GCTNAGC 1 cut(s) 1002
BspACI CCGC 7 cut(s) 11, 148, 479, 615, 1051, 1262, 1413
BspANI GGCC 3 cut(s) 797, 966, 1040
BspCNI CTCAG 3 cut(s) 915, 994, 1111
BspFNI CGCG 3 cut(s) 111, 150, 617
BspLI GGNNCC 3 cut(s) 465, 913, 966
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 3 cut(s) 169, 191, 221
BspQI GCTCTTC 1 cut(s) 120
BsrI ACTGG 2 cut(s) 537, 833
BssECI CCNNGG 2 cut(s) 363, 423
BssMI GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
BssNI GRCGYC 1 cut(s) 544
BssSI CACGAG 1 cut(s) 1143
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 1143
Bst2UI CCWGG 4 cut(s) 231, 365, 996, 1037
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 4 cut(s) 120, 567, 1080, 1182
BstACI GRCGYC 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 977
BstDEI CTNAG 6 cut(s) 710, 923, 971, 1002, 1119, 1242
BstF5I GGATG 3 cut(s) 186, 688, 775
BstFNI CGCG 3 cut(s) 111, 150, 617
BstH2I RGCGCY 1 cut(s) 1201
BstHHI GCGC 3 cut(s) 113, 374, 1200
BstKTI GATC 8 cut(s) 69, 105, 177, 186, 229, 403, 520, 625
BstMAI GTCTC 2 cut(s) 488, 1128
BstMBI GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
BstMWI GCNNNNNNNGC 7 cut(s) 139, 425, 659, 713, 722, 972, 1341
BstNI CCWGG 4 cut(s) 231, 365, 996, 1037
BstSCI CCNGG 4 cut(s) 229, 363, 994, 1035
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 968
BstUI CGCG 3 cut(s) 111, 150, 617
BstV1I GCAGC 3 cut(s) 547, 637, 734
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 3 cut(s) 797, 966, 1040
BtsCI GGATG 3 cut(s) 186, 688, 775
BtsIMutI CAGTG 1 cut(s) 544
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 1 cut(s) 977
CaiI CAGNNNCTG 1 cut(s) 1001
CfoI GCGC 3 cut(s) 113, 374, 1200
Cfr13I GGNCC 5 cut(s) 79, 463, 912, 964, 965
CseI GACGC 2 cut(s) 533, 569
Csp6I GTAC 2 cut(s) 1054, 1192
CviAII CATG 6 cut(s) 277, 689, 820, 1057, 1063, 1336
CviQI GTAC 2 cut(s) 1054, 1192
DdeI CTNAG 6 cut(s) 710, 923, 971, 1002, 1119, 1242
DpnI GATC 8 cut(s) 68, 104, 176, 185, 228, 402, 519, 624
DpnII GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 4 cut(s) 120, 567, 1080, 1182
EarI CTCTTC 4 cut(s) 120, 567, 1080, 1182
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 423
Eco147I AGGCCT 1 cut(s) 797
Eco24I GRGCYC 2 cut(s) 213, 968
Eco47I GGWCC 3 cut(s) 79, 463, 912
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 79, 912
EcoRII CCWGG 4 cut(s) 229, 363, 994, 1035
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 2 cut(s) 213, 968
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 6 cut(s) 280, 692, 823, 1060, 1066, 1339
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 925
FatI CATG 6 cut(s) 276, 688, 819, 1056, 1062, 1335
FauI CCCGC 1 cut(s) 1406
FauNDI CATATG 1 cut(s) 765
FbaI TGATCA 2 cut(s) 400, 622
Fnu4HI GCNGC 4 cut(s) 561, 615, 651, 723
FokI GGATG 3 cut(s) 173, 695, 782
FriOI GRGCYC 2 cut(s) 213, 968
Fsp4HI GCNGC 4 cut(s) 561, 615, 651, 723
GlaI GCGC 3 cut(s) 112, 373, 1199
GluI GCNGC 4 cut(s) 561, 615, 651, 723
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 1201
HaeIII GGCC 3 cut(s) 797, 966, 1040
HgaI GACGC 2 cut(s) 533, 569
HhaI GCGC 3 cut(s) 113, 374, 1200
Hin1I GRCGYC 1 cut(s) 544
Hin1II CATG 6 cut(s) 280, 692, 823, 1060, 1066, 1339
Hin6I GCGC 3 cut(s) 111, 372, 1198
HinP1I GCGC 3 cut(s) 111, 372, 1198
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 7 cut(s) 433, 458, 733, 850, 1070, 1121, 1237
HphI GGTGA 1 cut(s) 836
Hpy188I TCNGA 7 cut(s) 127, 252, 342, 386, 463, 907, 948
Hpy188III TCNNGA 6 cut(s) 82, 169, 190, 300, 834, 854
Hpy99I CGWCG 2 cut(s) 74, 601
HpyAV CCTTC 7 cut(s) 70, 479, 499, 773, 902, 925, 1240
HpyCH4III ACNGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 425, 659, 713, 722, 972, 1341
HpyF3I CTNAG 6 cut(s) 710, 923, 971, 1002, 1119, 1242
Hsp92I GRCGYC 1 cut(s) 544
Hsp92II CATG 6 cut(s) 280, 692, 823, 1060, 1066, 1339
HspAI GCGC 3 cut(s) 111, 372, 1198
Ksp22I TGATCA 2 cut(s) 400, 622
Kzo9I GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 24, 659, 1215
Lsp1109I GCAGC 3 cut(s) 547, 637, 734
LweI GCATC 2 cut(s) 36, 567
MaeIII GTNAC 3 cut(s) 545, 670, 824
MalI GATC 8 cut(s) 68, 104, 176, 185, 228, 402, 519, 624
MboI GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
MboII GAAGA 7 cut(s) 107, 184, 265, 355, 584, 1097, 1199
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 4 cut(s) 40, 213, 709, 968
MluCI AATT 6 cut(s) 14, 134, 474, 511, 525, 1230
MlyI GAGTC 4 cut(s) 467, 742, 1064, 1130
MmeI TCCRAC 4 cut(s) 332, 441, 637, 1071
MnlI CCTC 8 cut(s) 14, 104, 250, 692, 918, 1139, 1317, 1403
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 861
MseI TTAA 6 cut(s) 204, 501, 639, 792, 1233, 1278
MslI CAYNNNNRTG 2 cut(s) 768, 1061
MspA1I CMGCKG 1 cut(s) 979
MspR9I CCNGG 4 cut(s) 231, 365, 996, 1037
Mva1269I GAATGC 3 cut(s) 199, 421, 1407
MvaI CCWGG 4 cut(s) 231, 365, 996, 1037
MvnI CGCG 3 cut(s) 111, 150, 617
MwoI GCNNNNNNNGC 7 cut(s) 139, 425, 659, 713, 722, 972, 1341
NdeI CATATG 1 cut(s) 765
NdeII GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
NlaIII CATG 6 cut(s) 280, 692, 823, 1060, 1066, 1339
NlaIV GGNNCC 3 cut(s) 465, 913, 966
NmuCI GTSAC 2 cut(s) 545, 824
NsiI ATGCAT 1 cut(s) 331
PceI AGGCCT 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 1132
PctI GAATGC 3 cut(s) 199, 421, 1407
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 3 cut(s) 433, 850, 1237
PkrI GCNGC 4 cut(s) 562, 616, 652, 724
PleI GAGTC 4 cut(s) 466, 741, 1064, 1129
PpsI GAGTC 4 cut(s) 466, 741, 1064, 1129
PpuMI RGGWCCY 2 cut(s) 79, 912
PsiI TTATAA 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 2 cut(s) 79, 912
Psp6I CCWGG 4 cut(s) 229, 363, 994, 1035
PspGI CCWGG 4 cut(s) 229, 363, 994, 1035
PspN4I GGNNCC 3 cut(s) 465, 913, 966
PspOMI GGGCCC 1 cut(s) 964
PspPI GGNCC 5 cut(s) 79, 463, 912, 964, 965
PspPPI RGGWCCY 2 cut(s) 79, 912
PstI CTGCAG 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 174
PvuII CAGCTG 1 cut(s) 979
RsaI GTAC 2 cut(s) 1055, 1193
RsaNI GTAC 2 cut(s) 1054, 1192
RseI CAYNNNNRTG 2 cut(s) 768, 1061
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 6 cut(s) 204, 501, 639, 792, 1233, 1278
SatI GCNGC 4 cut(s) 561, 615, 651, 723
Sau3AI GATC 8 cut(s) 66, 102, 174, 183, 226, 400, 517, 622
Sau96I GGNCC 5 cut(s) 79, 463, 912, 964, 965
SchI GAGTC 4 cut(s) 467, 742, 1064, 1130
ScrFI CCNGG 4 cut(s) 231, 365, 996, 1037
SduI GDGCHC 4 cut(s) 40, 213, 709, 968
SfaNI GCATC 2 cut(s) 36, 567
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 3 cut(s) 79, 463, 912
SmiMI CAYNNNNRTG 2 cut(s) 768, 1061
SmlI CTYRAG 1 cut(s) 630
SmoI CTYRAG 1 cut(s) 630
Sse9I AATT 6 cut(s) 14, 134, 474, 511, 525, 1230
SseBI AGGCCT 1 cut(s) 797
SsiI CCGC 7 cut(s) 11, 148, 479, 615, 1051, 1262, 1413
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 797
StyD4I CCNGG 4 cut(s) 229, 363, 994, 1035
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 2 cut(s) 69, 1073
TasI AATT 6 cut(s) 14, 134, 474, 511, 525, 1230
TatI WGTACW 1 cut(s) 1191
TauI GCSGC 1 cut(s) 617
TfiI GAWTC 3 cut(s) 433, 850, 1237
Tru1I TTAA 6 cut(s) 204, 501, 639, 792, 1233, 1278
Tru9I TTAA 6 cut(s) 204, 501, 639, 792, 1233, 1278
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 2 cut(s) 545, 824
TseI GCWGC 3 cut(s) 560, 650, 722
Tsp45I GTSAC 2 cut(s) 545, 824
TspDTI ATGAA 3 cut(s) 1098, 1238, 1373
TspGWI ACGGA 2 cut(s) 167, 426
TspRI CASTG 1 cut(s) 544
VpaK11BI GGWCC 3 cut(s) 79, 463, 912
XmnI GAANNNNTTC 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.