FvH4_2g33430

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009104
Physical Location & Seq
Reverse (-)
25080326 .. 25082600
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33430.t1

Sequence Viewer

Length: 1503 bp
ATGGTGTTGGCGGAGCTAGGAGGGAGCATCTCCCGTGCTCTCCAGCAGATGAGCAATGCGACCGTGATCGACGAGAAGGTCCTCAACGAGTGCCTCAATGAGATCACACGCGCTCTTCTCCAATCCGATGTGCAATTCAAGCTCGTCCGCGACATGCAGACCAATATCAAGAAGATCGTCAATTTGGAAGATCTCGCTGCTGGTCACAACAAGCGTCGCATCATCCAGAACGCCATTTTTAATGAGCTCTGCAAAATGCTTGATCCTGGGAAGCCATCCTTCACTCCCAAGAAGGGGAAGCCCAGCGTCGTTATGTTTGTTGGTTTGCAAGGGTCTGGAAAGACTACGACTTGTACGAAGTATGCTTATTATCATCAGAAGAAGGGCTGGAAGCCTGCCCTGGTGTGTGCGGATACTTTCCGAGCTGGTGCATTTGATCAGTTGAAGCAGAATGCTACCAAGGCGAAGATTCCGTTCTATGGAAGCTATATGGAGTCTGATCCTGTGAAGATTGCGGTGGAAGGAGTGGAGACTTTTAAGAAGGAGAATTGTGATCTTATAATTGTTGATACCAGTGGGCGTCACAAGCAGGAGGCTGCTCTTTTTGAGGAGATGCGGCAGGTGGCTGAAGCGACGAAACCGGATCTTGTTATATTTGTTATGGACAGCAGTATTGGTCAGGCTGCGTTTGATCAAGCTCAAGCGTTTAAGCAGAGTGCTGCGGTTGGTGCTGTTATTGTTACCAAAATGGATGGTCATGCAAAGGGAGGTGGTGCTCTTAGTGCTGTTGCGGCGACAAAGAGTCCTGTTATATTTATCGGAACAGGAGAGCATATGGATGAGTTTGAAGTTTTTGATGTTAAACCGTTTGTCAGCCGTCTCTTAGGCATGGGTGACTGGTCTGGGTTTATGGACAAGATTCAGGAAGTTGTTCCTAAACTGGACAACCAGCCAGAGCTTCTGCAAAAGCTTTCGGAAGGGACCTTCACGCTGAGGATTATGTATGAGCAGTTTCAGAATCTGCTTCAAATGGGTCCACTTAGCCAGCTTACTTCAATGCTTCCAGGTGCTGAGTTAATGCCTAAAGGTAATGATAAGGAAAGCCAGGCCAAAATCAAGCGGTACATGACCATGATGGACTCGATGACAAATGAAGAGTTGGACAGCACAAATACAAAAATCATTACCGAGTCTCGGATAGTGCGAATAGCACGAGGTTCTGGTCGTCTAGTGAGAGAAGTGATGGAAATGTTTGAAGAGTACAAGCGCCTTGCGAAAATATGGAGCAAAATGAAAGGACTTAAGATTCCCAAGAAGGGACCAATGAGTTCACTAAACCAAAACATGAATGCACAGCAAATGAGCAAAGTCCTACCTCCTCAGATGTTGAAGCAGATTGGTGGCATGGGTGGCTTACAGAACTTGATGAAGCAAATGGGTGGTATGGGTGGTTTGGGTTCTGCTAAAGATATGATGGGAATGCTCGGAGGCGGGGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

501

Amino Acids

55.15

Weight (kDa)

9.16

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 6 - 83 3.4e-14 SRP54-type protein, helical bundle domain
SRP54 PF00448 101 - 296 8.9e-78 SRP54-type protein, GTPase domain
SRP_SPB PF02978 328 - 427 4.7e-27 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AarI CACCTGC 1 cut(s) 610
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 610
AccII CGCG 2 cut(s) 111, 150
AciI CCGC 9 cut(s) 11, 148, 410, 515, 616, 722, 791, 1120, 1491
AclWI GGATC 3 cut(s) 257, 494, 651
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 580
AfaI GTAC 3 cut(s) 355, 1124, 1262
AfiI CCNNNNNNNGG 5 cut(s) 293, 294, 479, 1194, 1316
AflII CTTAAG 1 cut(s) 1301
AgsI TTSAA 7 cut(s) 139, 445, 848, 1028, 1056, 1256, 1390
AhdI GACNNNNNGTC 1 cut(s) 801
AjnI CCWGG 4 cut(s) 265, 399, 1063, 1104
AluBI AGCT 9 cut(s) 16, 142, 247, 425, 486, 698, 958, 970, 1048
AluI AGCT 9 cut(s) 16, 142, 247, 425, 486, 698, 958, 970, 1048
Alw21I GWGCWC 3 cut(s) 40, 249, 778
Alw26I GTCTC 3 cut(s) 524, 884, 1197
AlwI GGATC 3 cut(s) 257, 494, 651
AlwNI CAGNNNCTG 2 cut(s) 1021, 1070
AoxI GGCC 1 cut(s) 1107
ApeKI GCWGC 4 cut(s) 197, 596, 683, 719
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 2 cut(s) 113, 1269
AspS9I GGNCC 4 cut(s) 79, 981, 1034, 1319
AsuHPI GGTGA 1 cut(s) 905
AvaII GGWCC 4 cut(s) 79, 981, 1034, 1319
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 1212
Bbv12I GWGCWC 3 cut(s) 40, 249, 778
BbvCI CCTCAGC 1 cut(s) 992
BbvI GCAGC 4 cut(s) 184, 583, 670, 706
BccI CCATC 5 cut(s) 283, 746, 1129, 1237, 1468
BceAI ACGGC 1 cut(s) 861
BciT130I CCWGG 4 cut(s) 267, 401, 1065, 1106
BciVI GTATCC 1 cut(s) 406
BclI TGATCA 2 cut(s) 436, 691
BcoDI GTCTC 3 cut(s) 524, 884, 1197
BfaI CTAG 2 cut(s) 17, 1229
BfoI RGCGCY 1 cut(s) 1270
BfrI CTTAAG 1 cut(s) 1301
BfuAI ACCTGC 1 cut(s) 610
BfuI GTATCC 1 cut(s) 406
BglII AGATCT 1 cut(s) 190
BisI GCNGC 6 cut(s) 198, 597, 617, 684, 720, 792
BlsI GCNGC 6 cut(s) 199, 598, 618, 685, 721, 793
Bme1390I CCNGG 4 cut(s) 267, 401, 1065, 1106
Bme18I GGWCC 4 cut(s) 79, 981, 1034, 1319
BmeRI GACNNNNNGTC 1 cut(s) 801
BmgT120I GGNCC 4 cut(s) 79, 981, 1034, 1319
BmiI GGNNCC 3 cut(s) 982, 1035, 1320
BmrFI CCNGG 4 cut(s) 267, 401, 1065, 1106
BmsI GCATC 3 cut(s) 36, 228, 603
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 266, 399, 459
BsaWI WCCGGW 1 cut(s) 640
Bsc4I CCNNNNNNNGG 5 cut(s) 293, 294, 479, 1194, 1316
Bse1I ACTGG 3 cut(s) 573, 902, 945
Bse3DI GCAATG 1 cut(s) 61
BseBI CCWGG 4 cut(s) 267, 401, 1065, 1106
BseDI CCNNGG 3 cut(s) 266, 399, 459
BseGI GGATG 4 cut(s) 222, 275, 757, 844
BseLI CCNNNNNNNGG 5 cut(s) 293, 294, 479, 1194, 1316
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 3 cut(s) 983, 1062, 1394
BseNI ACTGG 3 cut(s) 573, 902, 945
BseRI GAGGAG 2 cut(s) 623, 1368
BseXI GCAGC 4 cut(s) 184, 583, 670, 706
BseYI CCCAGC 1 cut(s) 302
Bsh1236I CGCG 2 cut(s) 111, 150
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 1109
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 3 cut(s) 40, 249, 778
BsiSI CCGG 1 cut(s) 641
BslFI GGGAC 2 cut(s) 994, 1332
BslI CCNNNNNNNGG 5 cut(s) 293, 294, 479, 1194, 1316
BsmAI GTCTC 3 cut(s) 524, 884, 1197
BsmBI CGTCTC 1 cut(s) 884
BsmFI GGGAC 2 cut(s) 994, 1332
BsmI GAATGC 3 cut(s) 457, 1354, 1485
BsnI GGCC 1 cut(s) 1109
Bsp1286I GDGCHC 3 cut(s) 40, 249, 778
BspACI CCGC 9 cut(s) 11, 148, 410, 515, 616, 722, 791, 1120, 1491
BspANI GGCC 1 cut(s) 1109
BspCNI CTCAG 3 cut(s) 984, 1063, 1393
BspFNI CGCG 2 cut(s) 111, 150
BspLI GGNNCC 3 cut(s) 982, 1035, 1320
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 3 cut(s) 257, 494, 651
BspQI GCTCTTC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 1301
BsrDI GCAATG 1 cut(s) 61
BsrI ACTGG 3 cut(s) 573, 902, 945
BssECI CCNNGG 3 cut(s) 266, 399, 459
BssNI GRCGYC 1 cut(s) 580
BssSI CACGAG 1 cut(s) 1212
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 1212
Bst2UI CCWGG 4 cut(s) 267, 401, 1065, 1106
Bst4CI ACNGT 2 cut(s) 64, 867
Bst6I CTCTTC 3 cut(s) 120, 1149, 1251
BstACI GRCGYC 1 cut(s) 580
BstAFI CTTAAG 1 cut(s) 1301
BstC8I GCNNGC 2 cut(s) 396, 1046
BstDEI CTNAG 6 cut(s) 779, 883, 992, 1040, 1071, 1380
BstF5I GGATG 4 cut(s) 222, 275, 757, 844
BstFNI CGCG 2 cut(s) 111, 150
BstH2I RGCGCY 1 cut(s) 1270
BstHHI GCGC 2 cut(s) 113, 1269
BstMAI GTCTC 3 cut(s) 524, 884, 1197
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 7 cut(s) 139, 461, 586, 728, 782, 791, 1410
BstNI CCWGG 4 cut(s) 267, 401, 1065, 1106
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 265, 399, 1063, 1104
BstUI CGCG 2 cut(s) 111, 150
BstV1I GCAGC 4 cut(s) 184, 583, 670, 706
BstX2I RGATCY 2 cut(s) 190, 643
BstYI RGATCY 2 cut(s) 190, 643
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 1 cut(s) 1109
BtsCI GGATG 4 cut(s) 222, 275, 757, 844
BtsIMutI CAGTG 1 cut(s) 580
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 2 cut(s) 396, 1046
CaiI CAGNNNCTG 2 cut(s) 1021, 1070
CfoI GCGC 2 cut(s) 113, 1269
Cfr13I GGNCC 4 cut(s) 79, 981, 1034, 1319
CseI GACGC 3 cut(s) 203, 295, 569
Csp6I GTAC 3 cut(s) 354, 1123, 1261
CviAII CATG 7 cut(s) 154, 758, 889, 1126, 1132, 1345, 1405
CviQI GTAC 3 cut(s) 354, 1123, 1261
DdeI CTNAG 6 cut(s) 779, 883, 992, 1040, 1071, 1380
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 801
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 3 cut(s) 120, 1149, 1251
Eam1105I GACNNNNNGTC 1 cut(s) 801
EarI CTCTTC 3 cut(s) 120, 1149, 1251
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 4 cut(s) 79, 981, 1034, 1319
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 648
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 2 cut(s) 79, 981
EcoRII CCWGG 4 cut(s) 265, 399, 1063, 1104
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 884
FaeI CATG 7 cut(s) 157, 761, 892, 1129, 1135, 1348, 1408
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 994, 1332
FatI CATG 7 cut(s) 153, 757, 888, 1125, 1131, 1344, 1404
FauI CCCGC 1 cut(s) 1484
FauNDI CATATG 1 cut(s) 834
FbaI TGATCA 2 cut(s) 436, 691
Fnu4HI GCNGC 6 cut(s) 198, 597, 617, 684, 720, 792
FokI GGATG 4 cut(s) 209, 262, 764, 851
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 6 cut(s) 198, 597, 617, 684, 720, 792
FspBI CTAG 2 cut(s) 17, 1229
GlaI GCGC 2 cut(s) 112, 1268
GluI GCNGC 6 cut(s) 198, 597, 617, 684, 720, 792
GsaI CCCAGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 1270
HaeIII GGCC 1 cut(s) 1109
HapII CCGG 1 cut(s) 641
HgaI GACGC 3 cut(s) 203, 295, 569
HhaI GCGC 2 cut(s) 113, 1269
Hin1I GRCGYC 1 cut(s) 580
Hin1II CATG 7 cut(s) 157, 761, 892, 1129, 1135, 1348, 1408
Hin6I GCGC 2 cut(s) 111, 1267
HinP1I GCGC 2 cut(s) 111, 1267
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 8 cut(s) 469, 494, 802, 919, 1018, 1139, 1190, 1306
HpaII CCGG 1 cut(s) 641
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 2 cut(s) 1037, 1331
Hpy188III TCNNGA 4 cut(s) 169, 226, 336, 923
Hpy8I GTNNAC 2 cut(s) 1037, 1331
Hpy99I CGWCG 4 cut(s) 74, 219, 311, 637
HpyAV CCTTC 9 cut(s) 70, 286, 289, 376, 515, 535, 971, 994, 1309
HpyCH4III ACNGT 2 cut(s) 64, 867
HpyCH4V TGCA 8 cut(s) 133, 157, 252, 328, 431, 761, 964, 1352
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 461, 586, 728, 782, 791, 1410
HpyF3I CTNAG 6 cut(s) 779, 883, 992, 1040, 1071, 1380
Hsp92I GRCGYC 1 cut(s) 580
Hsp92II CATG 7 cut(s) 157, 761, 892, 1129, 1135, 1348, 1408
HspAI GCGC 2 cut(s) 111, 1267
Ksp22I TGATCA 2 cut(s) 436, 691
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 13, 24, 1284
Lsp1109I GCAGC 4 cut(s) 184, 583, 670, 706
LweI GCATC 3 cut(s) 36, 228, 603
MaeI CTAG 2 cut(s) 17, 1229
MaeIII GTNAC 4 cut(s) 203, 581, 739, 893
MboII GAAGA 8 cut(s) 107, 184, 200, 391, 478, 520, 1166, 1268
MflI RGATCY 2 cut(s) 190, 643
MhlI GDGCHC 3 cut(s) 40, 249, 778
MluCI AATT 4 cut(s) 134, 181, 547, 561
MlyI GAGTC 4 cut(s) 503, 811, 1133, 1199
MmeI TCCRAC 1 cut(s) 1140
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 6 cut(s) 240, 537, 708, 861, 1076, 1302
MslI CAYNNNNRTG 2 cut(s) 837, 1130
MspCI CTTAAG 1 cut(s) 1301
MspI CCGG 1 cut(s) 641
MspR9I CCNGG 4 cut(s) 267, 401, 1065, 1106
Mva1269I GAATGC 3 cut(s) 457, 1354, 1485
MvaI CCWGG 4 cut(s) 267, 401, 1065, 1106
MvnI CGCG 2 cut(s) 111, 150
MwoI GCNNNNNNNGC 7 cut(s) 139, 461, 586, 728, 782, 791, 1410
NdeI CATATG 1 cut(s) 834
NlaIII CATG 7 cut(s) 157, 761, 892, 1129, 1135, 1348, 1408
NlaIV GGNNCC 3 cut(s) 982, 1035, 1320
NmuCI GTSAC 3 cut(s) 203, 581, 893
NspI RCATGY 1 cut(s) 157
PaqCI CACCTGC 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 2 cut(s) 353, 1201
PctI GAATGC 3 cut(s) 457, 1354, 1485
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 4 cut(s) 469, 919, 1018, 1306
PkrI GCNGC 6 cut(s) 199, 598, 618, 685, 721, 793
PleI GAGTC 4 cut(s) 502, 810, 1133, 1198
PpsI GAGTC 4 cut(s) 502, 810, 1133, 1198
PpuMI RGGWCCY 2 cut(s) 79, 981
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 2 cut(s) 79, 981
Psp6I CCWGG 4 cut(s) 265, 399, 1063, 1104
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 4 cut(s) 265, 399, 1063, 1104
PspN4I GGNNCC 3 cut(s) 982, 1035, 1320
PspPI GGNCC 4 cut(s) 79, 981, 1034, 1319
PspPPI RGGWCCY 2 cut(s) 79, 981
PstNI CAGNNNCTG 2 cut(s) 1021, 1070
PsuI RGATCY 2 cut(s) 190, 643
RsaI GTAC 3 cut(s) 355, 1124, 1262
RsaNI GTAC 3 cut(s) 354, 1123, 1261
RseI CAYNNNNRTG 2 cut(s) 837, 1130
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 6 cut(s) 240, 537, 708, 861, 1076, 1302
SatI GCNGC 6 cut(s) 198, 597, 617, 684, 720, 792
Sau96I GGNCC 4 cut(s) 79, 981, 1034, 1319
SchI GAGTC 4 cut(s) 503, 811, 1133, 1199
ScrFI CCNGG 4 cut(s) 267, 401, 1065, 1106
SduI GDGCHC 3 cut(s) 40, 249, 778
SfaNI GCATC 3 cut(s) 36, 228, 603
SinI GGWCC 4 cut(s) 79, 981, 1034, 1319
SmiMI CAYNNNNRTG 2 cut(s) 837, 1130
SmlI CTYRAG 2 cut(s) 699, 1301
SmoI CTYRAG 2 cut(s) 699, 1301
Sse9I AATT 4 cut(s) 134, 181, 547, 561
SsiI CCGC 9 cut(s) 11, 148, 410, 515, 616, 722, 791, 1120, 1491
SspMI CTAG 2 cut(s) 17, 1229
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 4 cut(s) 265, 399, 1063, 1104
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 64, 867
TaqI TCGA 2 cut(s) 69, 1142
TasI AATT 4 cut(s) 134, 181, 547, 561
TatI WGTACW 1 cut(s) 1260
TauI GCSGC 2 cut(s) 619, 794
TfiI GAWTC 4 cut(s) 469, 919, 1018, 1306
Tru1I TTAA 6 cut(s) 240, 537, 708, 861, 1076, 1302
Tru9I TTAA 6 cut(s) 240, 537, 708, 861, 1076, 1302
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 3 cut(s) 203, 581, 893
TseI GCWGC 4 cut(s) 197, 596, 683, 719
Tsp45I GTSAC 3 cut(s) 203, 581, 893
TspDTI ATGAA 4 cut(s) 1167, 1307, 1361, 1442
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 1301
VpaK11BI GGWCC 4 cut(s) 79, 981, 1034, 1319
XceI RCATGY 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 2 cut(s) 17, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.