Rw1G037680

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009104
Physical Location & Seq
Forward (+)
65305480 .. 65306974
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037680.1

Sequence Viewer

Length: 1404 bp
ATGGTGTTAGCGGAATTAGGAGGGAGCATCTCCCGTGCTCTCCAGCAGATGAGCAATGCCACCGTGATCGACGAGAAGGTCCTGAACGAGTGCCTCAACGAGATCACACGCGCTCTTCTCCAATCCGATGTGCAATTCAAGCTCGTCCGCGATATGCAGACCAATATCAAGAAGATCGTCAATTTGGAGGATCTCGCTGCCGGTCACAACAAGCGTAGGATCATCCAGAATGCTATTTTTAATGAGCTCTGCAAAATGCTTGATCCAGGGAAACCCTCTTTCACTCCGAAGAAAGGAAAACCGAGTGTGGTCATGTTTGTTGGTTTGCAAGGGTCTGGAAAGACTACAACTTGCACAAAATATGCATATTATCATCAGAAGAAAGGTTGGAAACCTGCCCTGGTGTGCGCTGATACTTTCCGAGCTGGTGCATTTGATCAGTTGAAGCAGAATGCTACCAAGGCAAAGATTCCGTTTTATGGAAGCTATATGGAGTCGGACCCTGTGAAAATTGCGGTGGAAGGAGTGGAGACTTTTAAGAAGGAGAATTGCGATCTTATAATTGTTGATACCAGTGGGCGTCACAAACAGGAAGCTGCTCTTTTTGAAGAGATGCGTCAGGTGGCCGAAGCGACGAAACCAGACCTTGTTGTATTTGTCATGGATAGCAGTATTGGTCAGGCCGCGTTTGATCAAGCTCAAGCATTTAAGCAAAGTGCTGCAGTTGGAGCTGTTATTGTTACCAAAATGGATGGTCATGCAAAGGGAGGTGGTGCTCTTAGTGCTGTTGCAGCAACAAAGAGTCCTGTTATATTTATTGGAACAGGAGAGCATATGGATGAGTTTGAAGGGTTTGATGTTAAGGCCTTTGTCAGCCGTCTATTAGGCATGGGTGACTGGTCTGGATTTATGGACAAGATTCAGGAAGTTGTTCCTAAACTTGACAACCAGCCAGAGCTTCTGCAAAAGCTTTCAGAAGGGACCTTCACGCTGAGGATTATGTATGAGCAGTTTCAGAACTTGCTTCAAATGGGCCCACTTAGCCAGCTGACTTCAATGCTTCCAGAGTTGGACAGCACAAATACAAAAATCATTACTGAGTCTCGTATAGTGCGAATAGCACGAGGTTCTGGTCGTCTGGTAAGAGAAGTGATGGAGATGTTTGAAGAGTACAAGCGCCTTGCTAAAATATGGAGCAAAATGAAAGGAATTAAGATTCCTAAGAAGGGTGGTATCAGTCCACTAAACCAAAACTTTAATGCACAGCAAATGAGCAAAGTCCTACCTCCCCAGATGTTGAAGCAGATTGGTGGCATGGGTGGCTTACAGAACTTGATGAAGCAAATGGGTTCAATGGGTTCAGCTAAAGAAATGATGGGAATGCTTGGAGGCGGGGACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

467

Amino Acids

51.43

Weight (kDa)

9.2

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP54_N PF02881 6 - 83 3.1e-14 SRP54-type protein, helical bundle domain
SRP54 PF00448 101 - 296 3.4e-77 SRP54-type protein, GTPase domain
SRP_SPB PF02978 328 - 397 1.1e-09 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009104)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15310 AT1G48900 AT1G48900 AT5G49500
fragaria_vesca FvH4_2g33430 FvH4_5g33730 FvH4_6g09610
malus_domestica MD17G1097300.v1.1 MD17G1213700.v1.1
prunus_persica Prupe.3G089600_v2.0.a1
pyrus_communis pycom17g21860
rosa_chinensis RchiOBHm_Chr6g0309101
rosa_laevigata RLG00000010589
rosa_multiflora Rmu_co8447547.1_g000001
rosa_roxburghii Rroxscaffold_7G00159650
rosa_rugosa Rorug06G0369800
rosa_samantha Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200
rosa_wichuraiana Rw1G037680 Rw6G042010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AasI GACNNNNNNGTC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 403
AccII CGCG 3 cut(s) 111, 150, 686
AciI CCGC 5 cut(s) 11, 148, 515, 684, 1392
AclWI GGATC 3 cut(s) 198, 227, 257
AcoI YGGCCR 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 580
AfaI GTAC 1 cut(s) 1172
AfiI CCNNNNNNNGG 3 cut(s) 293, 479, 1226
AgsI TTSAA 9 cut(s) 139, 445, 608, 848, 1028, 1056, 1166, 1300, 1353
AjnI CCWGG 2 cut(s) 265, 399
Alw21I GWGCWC 3 cut(s) 40, 249, 778
Alw26I GTCTC 2 cut(s) 524, 1107
AlwI GGATC 3 cut(s) 198, 227, 257
AoxI GGCC 4 cut(s) 624, 681, 864, 1033
ApaI GGGCCC 1 cut(s) 1037
ApeKI GCWGC 4 cut(s) 197, 596, 719, 791
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 3 cut(s) 113, 410, 1179
AspS9I GGNCC 5 cut(s) 79, 499, 981, 1033, 1034
AsuHPI GGTGA 1 cut(s) 905
AvaII GGWCC 3 cut(s) 79, 499, 981
BaeGI GKGCMC 1 cut(s) 1037
BanII GRGCYC 2 cut(s) 249, 1037
BauI CACGAG 1 cut(s) 1122
Bbv12I GWGCWC 3 cut(s) 40, 249, 778
BbvCI CCTCAGC 1 cut(s) 992
BbvI GCAGC 4 cut(s) 184, 583, 706, 803
BccI CCATC 3 cut(s) 746, 1147, 1369
BceAI ACGGC 1 cut(s) 861
BciT130I CCWGG 2 cut(s) 267, 401
BclI TGATCA 2 cut(s) 436, 691
BcoDI GTCTC 2 cut(s) 524, 1107
BfmI CTRYAG 1 cut(s) 720
BfoI RGCGCY 1 cut(s) 1180
BfuAI ACCTGC 1 cut(s) 403
BisI GCNGC 5 cut(s) 198, 597, 684, 720, 792
BlsI GCNGC 5 cut(s) 199, 598, 685, 721, 793
Bme1390I CCNGG 2 cut(s) 267, 401
Bme18I GGWCC 3 cut(s) 79, 499, 981
BmgT120I GGNCC 5 cut(s) 79, 499, 981, 1033, 1034
BmiI GGNNCC 3 cut(s) 501, 982, 1035
BmrFI CCNGG 2 cut(s) 267, 401
BmsI GCATC 2 cut(s) 36, 603
BpmI CTGGAG 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 266, 399, 459
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 479, 1226
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 573, 902
Bse3DI GCAATG 1 cut(s) 61
BseBI CCWGG 2 cut(s) 267, 401
BseDI CCNNGG 3 cut(s) 266, 399, 459
BseGI GGATG 3 cut(s) 222, 757, 844
BseLI CCNNNNNNNGG 3 cut(s) 293, 479, 1226
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 983, 1089
BseNI ACTGG 2 cut(s) 573, 902
BseSI GKGCMC 1 cut(s) 1037
BseXI GCAGC 4 cut(s) 184, 583, 706, 803
Bsh1236I CGCG 3 cut(s) 111, 150, 686
BshFI GGCC 4 cut(s) 626, 683, 866, 1035
BsiHKAI GWGCWC 3 cut(s) 40, 249, 778
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 3 cut(s) 293, 479, 1226
BsmAI GTCTC 2 cut(s) 524, 1107
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 3 cut(s) 235, 457, 1386
BsnI GGCC 4 cut(s) 626, 683, 866, 1035
Bsp120I GGGCCC 1 cut(s) 1033
Bsp1286I GDGCHC 4 cut(s) 40, 249, 778, 1037
Bsp143I GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
BspACI CCGC 5 cut(s) 11, 148, 515, 684, 1392
BspANI GGCC 4 cut(s) 626, 683, 866, 1035
BspCNI CTCAG 2 cut(s) 984, 1090
BspFNI CGCG 3 cut(s) 111, 150, 686
BspLI GGNNCC 3 cut(s) 501, 982, 1035
BspMAI CTGCAG 1 cut(s) 724
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 3 cut(s) 198, 227, 257
BspQI GCTCTTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 2 cut(s) 573, 902
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 266, 399, 459
BssMI GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
BssNI GRCGYC 1 cut(s) 580
BssSI CACGAG 1 cut(s) 1122
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 1122
Bst2UI CCWGG 2 cut(s) 267, 401
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 3 cut(s) 120, 603, 1161
BstACI GRCGYC 1 cut(s) 580
BstC8I GCNNGC 1 cut(s) 1046
BstDEI CTNAG 5 cut(s) 779, 992, 1040, 1098, 1221
BstF5I GGATG 3 cut(s) 222, 757, 844
BstFNI CGCG 3 cut(s) 111, 150, 686
BstH2I RGCGCY 1 cut(s) 1180
BstHHI GCGC 3 cut(s) 113, 410, 1179
BstKTI GATC 9 cut(s) 69, 105, 177, 193, 222, 265, 439, 556, 694
BstMAI GTCTC 2 cut(s) 524, 1107
BstMBI GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
BstMWI GCNNNNNNNGC 7 cut(s) 139, 461, 728, 782, 791, 1041, 1320
BstNI CCWGG 2 cut(s) 267, 401
BstSCI CCNGG 2 cut(s) 265, 399
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 1 cut(s) 1037
BstUI CGCG 3 cut(s) 111, 150, 686
BstV1I GCAGC 4 cut(s) 184, 583, 706, 803
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 4 cut(s) 626, 683, 866, 1035
BtsCI GGATG 3 cut(s) 222, 757, 844
BtsIMutI CAGTG 1 cut(s) 580
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 1046
CfoI GCGC 3 cut(s) 113, 410, 1179
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 79, 499, 981, 1033, 1034
CseI GACGC 2 cut(s) 569, 605
Csp6I GTAC 1 cut(s) 1171
CviAII CATG 5 cut(s) 313, 661, 758, 889, 1315
CviQI GTAC 1 cut(s) 1171
DdeI CTNAG 5 cut(s) 779, 992, 1040, 1098, 1221
DpnI GATC 9 cut(s) 68, 104, 176, 192, 221, 264, 438, 555, 693
DpnII GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 3 cut(s) 120, 603, 1161
EarI CTCTTC 3 cut(s) 120, 603, 1161
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 866
Eco24I GRGCYC 2 cut(s) 249, 1037
Eco47I GGWCC 3 cut(s) 79, 499, 981
Eco53kI GAGCTC 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 2 cut(s) 79, 981
EcoRII CCWGG 2 cut(s) 265, 399
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 2 cut(s) 249, 1037
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 5 cut(s) 316, 664, 761, 892, 1318
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 994
FatI CATG 5 cut(s) 312, 660, 757, 888, 1314
FauI CCCGC 1 cut(s) 1385
FauNDI CATATG 1 cut(s) 834
FbaI TGATCA 2 cut(s) 436, 691
Fnu4HI GCNGC 5 cut(s) 198, 597, 684, 720, 792
FokI GGATG 3 cut(s) 209, 764, 851
FriOI GRGCYC 2 cut(s) 249, 1037
Fsp4HI GCNGC 5 cut(s) 198, 597, 684, 720, 792
GlaI GCGC 3 cut(s) 112, 409, 1178
GluI GCNGC 5 cut(s) 198, 597, 684, 720, 792
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 1180
HaeIII GGCC 4 cut(s) 626, 683, 866, 1035
HapII CCGG 1 cut(s) 201
HgaI GACGC 2 cut(s) 569, 605
HhaI GCGC 3 cut(s) 113, 410, 1179
Hin1I GRCGYC 1 cut(s) 580
Hin1II CATG 5 cut(s) 316, 664, 761, 892, 1318
Hin6I GCGC 3 cut(s) 111, 408, 1177
HinP1I GCGC 3 cut(s) 111, 408, 1177
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 6 cut(s) 469, 494, 802, 919, 1100, 1216
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 1 cut(s) 1241
Hpy188I TCNGA 7 cut(s) 127, 288, 378, 422, 499, 976, 1017
Hpy188III TCNNGA 7 cut(s) 82, 169, 226, 336, 903, 923, 1064
Hpy8I GTNNAC 1 cut(s) 1241
Hpy99I CGWCG 2 cut(s) 74, 637
HpyAV CCTTC 7 cut(s) 70, 515, 535, 842, 971, 994, 1219
HpyCH4III ACNGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 461, 728, 782, 791, 1041, 1320
HpyF3I CTNAG 5 cut(s) 779, 992, 1040, 1098, 1221
Hsp92I GRCGYC 1 cut(s) 580
Hsp92II CATG 5 cut(s) 316, 664, 761, 892, 1318
HspAI GCGC 3 cut(s) 111, 408, 1177
Ksp22I TGATCA 2 cut(s) 436, 691
Kzo9I GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 24, 728, 1194
Lsp1109I GCAGC 4 cut(s) 184, 583, 706, 803
LweI GCATC 2 cut(s) 36, 603
MaeIII GTNAC 4 cut(s) 203, 581, 739, 893
MalI GATC 9 cut(s) 68, 104, 176, 192, 221, 264, 438, 555, 693
MboI GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
MboII GAAGA 6 cut(s) 107, 184, 301, 391, 620, 1178
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 4 cut(s) 40, 249, 778, 1037
MluCI AATT 7 cut(s) 14, 134, 181, 510, 547, 561, 1209
MlyI GAGTC 3 cut(s) 503, 811, 1109
MmeI TCCRAC 4 cut(s) 368, 477, 706, 1050
MnlI CCTC 9 cut(s) 14, 104, 181, 286, 761, 987, 1118, 1296, 1382
Mph1103I ATGCAT 1 cut(s) 367
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 6 cut(s) 240, 537, 708, 861, 1212, 1257
MslI CAYNNNNRTG 1 cut(s) 837
MspA1I CMGCKG 1 cut(s) 1048
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 267, 401
Mva1269I GAATGC 3 cut(s) 235, 457, 1386
MvaI CCWGG 2 cut(s) 267, 401
MvnI CGCG 3 cut(s) 111, 150, 686
MwoI GCNNNNNNNGC 7 cut(s) 139, 461, 728, 782, 791, 1041, 1320
NdeI CATATG 1 cut(s) 834
NdeII GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
NlaIII CATG 5 cut(s) 316, 664, 761, 892, 1318
NlaIV GGNNCC 3 cut(s) 501, 982, 1035
NmuCI GTSAC 3 cut(s) 203, 581, 893
NsiI ATGCAT 1 cut(s) 367
PceI AGGCCT 1 cut(s) 866
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 1111
PctI GAATGC 3 cut(s) 235, 457, 1386
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 3 cut(s) 469, 919, 1216
PkrI GCNGC 5 cut(s) 199, 598, 685, 721, 793
PleI GAGTC 3 cut(s) 502, 810, 1108
PpsI GAGTC 3 cut(s) 502, 810, 1108
PpuMI RGGWCCY 2 cut(s) 79, 981
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 2 cut(s) 79, 981
Psp6I CCWGG 2 cut(s) 265, 399
PspGI CCWGG 2 cut(s) 265, 399
PspN4I GGNNCC 3 cut(s) 501, 982, 1035
PspOMI GGGCCC 1 cut(s) 1033
PspPI GGNCC 5 cut(s) 79, 499, 981, 1033, 1034
PspPPI RGGWCCY 2 cut(s) 79, 981
PstI CTGCAG 1 cut(s) 724
PsuI RGATCY 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 1048
RsaI GTAC 1 cut(s) 1172
RsaNI GTAC 1 cut(s) 1171
RseI CAYNNNNRTG 1 cut(s) 837
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 6 cut(s) 240, 537, 708, 861, 1212, 1257
SatI GCNGC 5 cut(s) 198, 597, 684, 720, 792
Sau3AI GATC 9 cut(s) 66, 102, 174, 190, 219, 262, 436, 553, 691
Sau96I GGNCC 5 cut(s) 79, 499, 981, 1033, 1034
SchI GAGTC 3 cut(s) 503, 811, 1109
ScrFI CCNGG 2 cut(s) 267, 401
SduI GDGCHC 4 cut(s) 40, 249, 778, 1037
SfaNI GCATC 2 cut(s) 36, 603
SfcI CTRYAG 1 cut(s) 720
SinI GGWCC 3 cut(s) 79, 499, 981
SmiMI CAYNNNNRTG 1 cut(s) 837
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
Sse9I AATT 7 cut(s) 14, 134, 181, 510, 547, 561, 1209
SseBI AGGCCT 1 cut(s) 866
SsiI CCGC 5 cut(s) 11, 148, 515, 684, 1392
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 1 cut(s) 866
StyD4I CCNGG 2 cut(s) 265, 399
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 69
TasI AATT 7 cut(s) 14, 134, 181, 510, 547, 561, 1209
TatI WGTACW 1 cut(s) 1170
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 3 cut(s) 469, 919, 1216
Tru1I TTAA 6 cut(s) 240, 537, 708, 861, 1212, 1257
Tru9I TTAA 6 cut(s) 240, 537, 708, 861, 1212, 1257
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 3 cut(s) 203, 581, 893
TseI GCWGC 4 cut(s) 197, 596, 719, 791
Tsp45I GTSAC 3 cut(s) 203, 581, 893
TspDTI ATGAA 2 cut(s) 1217, 1352
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 79, 499, 981
XmnI GAANNNNTTC 1 cut(s) 930
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.