Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G15310 AT1G48900 AT1G48900 AT5G49500 |
| fragaria_vesca | FvH4_2g33430 FvH4_5g33730 FvH4_6g09610 |
| malus_domestica | MD17G1097300.v1.1 MD17G1213700.v1.1 |
| prunus_persica | Prupe.3G089600_v2.0.a1 |
| pyrus_communis | pycom17g21860 |
| rosa_chinensis | RchiOBHm_Chr6g0309101 |
| rosa_laevigata | RLG00000010589 |
| rosa_multiflora | Rmu_co8447547.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00159650 |
| rosa_rugosa | Rorug06G0369800 |
| rosa_samantha | Rh6AG482500 Rh6BG491500 Rh6CG496300 Rh6DG483200 |
| rosa_wichuraiana | Rw1G037680 Rw6G042010 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 560 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 77 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 479, 1284 |
| AccII | CGCG | 3 cut(s) | 34, 111, 150 |
| AciI | CCGC | 6 cut(s) | 148, 198, 515, 1123, 1382, 1413 |
| AclI | AACGTT | 1 cut(s) | 533 |
| AclWI | GGATC | 2 cut(s) | 257, 270 |
| AcoI | YGGCCR | 1 cut(s) | 1039 |
| AcuI | CTGAAG | 2 cut(s) | 648, 1412 |
| AcyI | GRCGYC | 1 cut(s) | 580 |
| AdeI | CACNNNGTG | 1 cut(s) | 351 |
| AfaI | GTAC | 5 cut(s) | 355, 1001, 1127, 1133, 1265 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 293, 294, 479, 1099, 1284, 1319 |
| AflIII | ACRYGT | 2 cut(s) | 350, 1128 |
| AgsI | TTSAA | 7 cut(s) | 134, 139, 445, 521, 538, 608, 848 |
| AjnI | CCWGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 114, 146, 1145, 1177 |
| Alw21I | GWGCWC | 4 cut(s) | 40, 778, 834, 1336 |
| Alw26I | GTCTC | 2 cut(s) | 884, 888 |
| Alw44I | GTGCAC | 1 cut(s) | 1332 |
| AlwI | GGATC | 2 cut(s) | 257, 270 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 503, 1390 |
| AoxI | GGCC | 2 cut(s) | 1039, 1090 |
| ApaLI | GTGCAC | 1 cut(s) | 1332 |
| ApeKI | GCWGC | 3 cut(s) | 596, 683, 791 |
| AseI | ATTAAT | 1 cut(s) | 1079 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 930 |
| AspLEI | GCGC | 4 cut(s) | 13, 113, 216, 1272 |
| AspS9I | GGNCC | 4 cut(s) | 79, 940, 1031, 1090 |
| AsuHPI | GGTGA | 1 cut(s) | 1334 |
| AvaII | GGWCC | 3 cut(s) | 79, 940, 1031 |
| BaeGI | GKGCMC | 1 cut(s) | 1336 |
| BalI | TGGCCA | 1 cut(s) | 1041 |
| BamHI | GGATCC | 1 cut(s) | 262 |
| BanII | GRGCYC | 1 cut(s) | 778 |
| BauI | CACGAG | 1 cut(s) | 1215 |
| Bbv12I | GWGCWC | 4 cut(s) | 40, 778, 834, 1336 |
| BbvI | GCAGC | 3 cut(s) | 583, 670, 778 |
| BccI | CCATC | 4 cut(s) | 746, 1132, 1249, 1453 |
| BceAI | ACGGC | 2 cut(s) | 861, 1077 |
| BciT130I | CCWGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| BclI | TGATCA | 2 cut(s) | 436, 691 |
| BcoDI | GTCTC | 2 cut(s) | 884, 888 |
| BfaI | CTAG | 1 cut(s) | 401 |
| BfoI | RGCGCY | 1 cut(s) | 1273 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 461 |
| BglII | AGATCT | 1 cut(s) | 643 |
| BisI | GCNGC | 5 cut(s) | 198, 597, 684, 792, 1414 |
| BlsI | GCNGC | 5 cut(s) | 199, 598, 685, 793, 1415 |
| Bme1390I | CCNGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| Bme18I | GGWCC | 3 cut(s) | 79, 940, 1031 |
| BmgT120I | GGNCC | 4 cut(s) | 79, 940, 1031, 1090 |
| BmiI | GGNNCC | 2 cut(s) | 264, 1032 |
| BmrFI | CCNGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| BmsI | GCATC | 2 cut(s) | 36, 1377 |
| BpmI | CTGGAG | 1 cut(s) | 26 |
| BpuEI | CTTGAG | 2 cut(s) | 684, 1289 |
| BsaAI | YACGTR | 1 cut(s) | 1199 |
| BsaHI | GRCGYC | 1 cut(s) | 580 |
| BsaJI | CCNNGG | 1 cut(s) | 266 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 293, 294, 479, 1099, 1284, 1319 |
| Bse118I | RCCGGY | 1 cut(s) | 200 |
| Bse1I | ACTGG | 2 cut(s) | 573, 902 |
| Bse3DI | GCAATG | 1 cut(s) | 61 |
| BseBI | CCWGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| BseDI | CCNNGG | 1 cut(s) | 266 |
| BseGI | GGATG | 2 cut(s) | 757, 1470 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 293, 294, 479, 1099, 1284, 1319 |
| BseMI | GCAATG | 1 cut(s) | 61 |
| BseNI | ACTGG | 2 cut(s) | 573, 902 |
| BseSI | GKGCMC | 1 cut(s) | 1336 |
| BseXI | GCAGC | 3 cut(s) | 583, 670, 778 |
| BseYI | CCCAGC | 1 cut(s) | 425 |
| BsgI | GTGCAG | 1 cut(s) | 429 |
| Bsh1236I | CGCG | 3 cut(s) | 34, 111, 150 |
| Bsh1285I | CGRYCG | 1 cut(s) | 63 |
| BshFI | GGCC | 2 cut(s) | 1041, 1092 |
| BsiEI | CGRYCG | 1 cut(s) | 63 |
| BsiHKAI | GWGCWC | 4 cut(s) | 40, 778, 834, 1336 |
| BsiSI | CCGG | 1 cut(s) | 201 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 293, 294, 479, 1099, 1284, 1319 |
| BsmAI | GTCTC | 2 cut(s) | 884, 888 |
| BsmBI | CGTCTC | 1 cut(s) | 884 |
| BsmI | GAATGC | 2 cut(s) | 457, 1357 |
| BsnI | GGCC | 2 cut(s) | 1041, 1092 |
| Bsp1286I | GDGCHC | 4 cut(s) | 40, 778, 834, 1336 |
| Bsp1407I | TGTACA | 2 cut(s) | 353, 1131 |
| Bsp143I | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| BspACI | CCGC | 6 cut(s) | 148, 198, 515, 1123, 1382, 1413 |
| BspANI | GGCC | 2 cut(s) | 1041, 1092 |
| BspFNI | CGCG | 3 cut(s) | 34, 111, 150 |
| BspHI | TCATGA | 2 cut(s) | 922, 1185 |
| BspLI | GGNNCC | 2 cut(s) | 264, 1032 |
| BspPI | GGATC | 2 cut(s) | 257, 270 |
| BsrDI | GCAATG | 1 cut(s) | 61 |
| BsrFI | RCCGGY | 1 cut(s) | 200 |
| BsrGI | TGTACA | 2 cut(s) | 353, 1131 |
| BsrI | ACTGG | 2 cut(s) | 573, 902 |
| BssAI | RCCGGY | 1 cut(s) | 200 |
| BssECI | CCNNGG | 1 cut(s) | 266 |
| BssMI | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| BssNI | GRCGYC | 1 cut(s) | 580 |
| BssSI | CACGAG | 1 cut(s) | 1215 |
| Bst2BI | CACGAG | 1 cut(s) | 1215 |
| Bst2UI | CCWGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| Bst4CI | ACNGT | 1 cut(s) | 64 |
| Bst6I | CTCTTC | 2 cut(s) | 1152, 1254 |
| BstACI | GRCGYC | 1 cut(s) | 580 |
| BstAUI | TGTACA | 2 cut(s) | 353, 1131 |
| BstBAI | YACGTR | 1 cut(s) | 1199 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 231, 396 |
| BstDEI | CTNAG | 4 cut(s) | 459, 465, 779, 883 |
| BstF5I | GGATG | 2 cut(s) | 757, 1470 |
| BstFNI | CGCG | 3 cut(s) | 34, 111, 150 |
| BstH2I | RGCGCY | 1 cut(s) | 1273 |
| BstHHI | GCGC | 4 cut(s) | 13, 113, 216, 1272 |
| BstKTI | GATC | 9 cut(s) | 105, 177, 193, 265, 439, 556, 646, 694, 1119 |
| BstMAI | GTCTC | 2 cut(s) | 884, 888 |
| BstMBI | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| BstMCI | CGRYCG | 1 cut(s) | 63 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 461, 782, 791, 1227, 1413, 1422 |
| BstNI | CCWGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| BstNSI | RCATGY | 1 cut(s) | 1132 |
| BstSCI | CCNGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| BstSLI | GKGCMC | 1 cut(s) | 1336 |
| BstUI | CGCG | 3 cut(s) | 34, 111, 150 |
| BstV1I | GCAGC | 3 cut(s) | 583, 670, 778 |
| BstX2I | RGATCY | 2 cut(s) | 262, 643 |
| BstYI | RGATCY | 2 cut(s) | 262, 643 |
| BsuRI | GGCC | 2 cut(s) | 1041, 1092 |
| BtsCI | GGATG | 2 cut(s) | 757, 1470 |
| BtsIMutI | CAGTG | 1 cut(s) | 580 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 231, 396 |
| CaiI | CAGNNNCTG | 2 cut(s) | 503, 1390 |
| CciI | TCATGA | 2 cut(s) | 922, 1185 |
| CfoI | GCGC | 4 cut(s) | 13, 113, 216, 1272 |
| Cfr10I | RCCGGY | 1 cut(s) | 200 |
| Cfr13I | GGNCC | 4 cut(s) | 79, 940, 1031, 1090 |
| CseI | GACGC | 2 cut(s) | 569, 605 |
| Csp6I | GTAC | 5 cut(s) | 354, 1000, 1126, 1132, 1264 |
| CviQI | GTAC | 5 cut(s) | 354, 1000, 1126, 1132, 1264 |
| DdeI | CTNAG | 4 cut(s) | 459, 465, 779, 883 |
| DpnI | GATC | 9 cut(s) | 104, 176, 192, 264, 438, 555, 645, 693, 1118 |
| DpnII | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| DraIII | CACNNNGTG | 1 cut(s) | 351 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 77 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 77 |
| EaeI | YGGCCR | 1 cut(s) | 1039 |
| Eam1104I | CTCTTC | 2 cut(s) | 1152, 1254 |
| EarI | CTCTTC | 2 cut(s) | 1152, 1254 |
| Ecl136II | GAGCTC | 1 cut(s) | 776 |
| Eco24I | GRGCYC | 1 cut(s) | 778 |
| Eco47I | GGWCC | 3 cut(s) | 79, 940, 1031 |
| Eco53kI | GAGCTC | 1 cut(s) | 776 |
| Eco57I | CTGAAG | 2 cut(s) | 648, 1412 |
| EcoICRI | GAGCTC | 1 cut(s) | 776 |
| EcoO109I | RGGNCCY | 1 cut(s) | 79 |
| EcoRII | CCWGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| EcoT22I | ATGCAT | 1 cut(s) | 367 |
| EcoT38I | GRGCYC | 1 cut(s) | 778 |
| Esp3I | CGTCTC | 1 cut(s) | 884 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 585, 617 |
| FauI | CCCGC | 1 cut(s) | 1389 |
| FbaI | TGATCA | 2 cut(s) | 436, 691 |
| Fnu4HI | GCNGC | 5 cut(s) | 198, 597, 684, 792, 1414 |
| FokI | GGATG | 2 cut(s) | 764, 1477 |
| FriOI | GRGCYC | 1 cut(s) | 778 |
| Fsp4HI | GCNGC | 5 cut(s) | 198, 597, 684, 792, 1414 |
| FspBI | CTAG | 1 cut(s) | 401 |
| GlaI | GCGC | 4 cut(s) | 12, 112, 215, 1271 |
| GluI | GCNGC | 5 cut(s) | 198, 597, 684, 792, 1414 |
| GsaI | CCCAGC | 1 cut(s) | 429 |
| GsuI | CTGGAG | 1 cut(s) | 26 |
| HaeII | RGCGCY | 1 cut(s) | 1273 |
| HaeIII | GGCC | 2 cut(s) | 1041, 1092 |
| HapII | CCGG | 1 cut(s) | 201 |
| HgaI | GACGC | 2 cut(s) | 569, 605 |
| HhaI | GCGC | 4 cut(s) | 13, 113, 216, 1272 |
| Hin1I | GRCGYC | 1 cut(s) | 580 |
| Hin6I | GCGC | 4 cut(s) | 11, 111, 214, 1270 |
| HinP1I | GCGC | 4 cut(s) | 11, 111, 214, 1270 |
| HindIII | AAGCTT | 2 cut(s) | 965, 1423 |
| HinfI | GANTC | 8 cut(s) | 469, 494, 802, 919, 1142, 1193, 1302, 1309 |
| HpaII | CCGG | 1 cut(s) | 201 |
| HphI | GGTGA | 1 cut(s) | 1334 |
| Hpy166II | GTNNAC | 3 cut(s) | 326, 354, 1334 |
| Hpy188I | TCNGA | 9 cut(s) | 127, 378, 499, 628, 973, 1014, 1238, 1314, 1472 |
| Hpy188III | TCNNGA | 9 cut(s) | 82, 169, 185, 336, 903, 923, 1186, 1306, 1341 |
| Hpy8I | GTNNAC | 3 cut(s) | 326, 354, 1334 |
| Hpy99I | CGWCG | 1 cut(s) | 74 |
| HpyAV | CCTTC | 5 cut(s) | 70, 285, 515, 968, 1312 |
| HpyCH4III | ACNGT | 1 cut(s) | 64 |
| HpyCH4IV | ACGT | 3 cut(s) | 350, 533, 1198 |
| HpyCH4V | TGCA | 9 cut(s) | 157, 252, 365, 410, 686, 761, 794, 1334, 1355 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 461, 782, 791, 1227, 1413, 1422 |
| HpyF3I | CTNAG | 4 cut(s) | 459, 465, 779, 883 |
| HpySE526I | ACGT | 3 cut(s) | 350, 533, 1198 |
| Hsp92I | GRCGYC | 1 cut(s) | 580 |
| HspAI | GCGC | 4 cut(s) | 11, 111, 214, 1270 |
| Ksp22I | TGATCA | 2 cut(s) | 436, 691 |
| Kzo9I | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| LmnI | GCTCC | 4 cut(s) | 24, 123, 773, 1287 |
| Lsp1109I | GCAGC | 3 cut(s) | 583, 670, 778 |
| LweI | GCATC | 2 cut(s) | 36, 1377 |
| MaeI | CTAG | 1 cut(s) | 401 |
| MaeII | ACGT | 3 cut(s) | 350, 533, 1198 |
| MaeIII | GTNAC | 4 cut(s) | 203, 581, 739, 1322 |
| MalI | GATC | 9 cut(s) | 104, 176, 192, 264, 438, 555, 645, 693, 1118 |
| MboI | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| MboII | GAAGA | 5 cut(s) | 184, 620, 1169, 1271, 1327 |
| MfeI | CAATTG | 2 cut(s) | 14, 1035 |
| MflI | RGATCY | 2 cut(s) | 262, 643 |
| MhlI | GDGCHC | 4 cut(s) | 40, 778, 834, 1336 |
| MlsI | TGGCCA | 1 cut(s) | 1041 |
| MluCI | AATT | 7 cut(s) | 14, 134, 510, 547, 561, 1035, 1076 |
| MluNI | TGGCCA | 1 cut(s) | 1041 |
| MlyI | GAGTC | 4 cut(s) | 503, 811, 1136, 1296 |
| MmeI | TCCRAC | 2 cut(s) | 368, 1143 |
| MnlI | CCTC | 6 cut(s) | 14, 104, 761, 984, 1211, 1466 |
| Mox20I | TGGCCA | 1 cut(s) | 1041 |
| Mph1103I | ATGCAT | 1 cut(s) | 367 |
| MroXI | GAANNNNTTC | 1 cut(s) | 930 |
| MscI | TGGCCA | 1 cut(s) | 1041 |
| MseI | TTAA | 4 cut(s) | 240, 861, 1023, 1079 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1133 |
| Msp20I | TGGCCA | 1 cut(s) | 1041 |
| MspI | CCGG | 1 cut(s) | 201 |
| MspR9I | CCNGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| MunI | CAATTG | 2 cut(s) | 14, 1035 |
| Mva1269I | GAATGC | 2 cut(s) | 457, 1357 |
| MvaI | CCWGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| MvnI | CGCG | 3 cut(s) | 34, 111, 150 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 461, 782, 791, 1227, 1413, 1422 |
| NdeII | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| NlaIV | GGNNCC | 2 cut(s) | 264, 1032 |
| NmuCI | GTSAC | 3 cut(s) | 203, 581, 1322 |
| NsiI | ATGCAT | 1 cut(s) | 367 |
| NspI | RCATGY | 1 cut(s) | 1132 |
| PagI | TCATGA | 2 cut(s) | 922, 1185 |
| PciI | ACATGT | 1 cut(s) | 1128 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 183, 1204, 1476 |
| PctI | GAATGC | 2 cut(s) | 457, 1357 |
| PdmI | GAANNNNTTC | 1 cut(s) | 930 |
| PfeI | GAWTC | 4 cut(s) | 469, 919, 1193, 1309 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 479, 1284 |
| PfoI | TCCNGGA | 1 cut(s) | 1060 |
| PkrI | GCNGC | 5 cut(s) | 199, 598, 685, 793, 1415 |
| PleI | GAGTC | 4 cut(s) | 502, 810, 1136, 1296 |
| PpsI | GAGTC | 4 cut(s) | 502, 810, 1136, 1296 |
| Ppu21I | YACGTR | 1 cut(s) | 1199 |
| PpuMI | RGGWCCY | 1 cut(s) | 79 |
| PscI | ACATGT | 1 cut(s) | 1128 |
| PshBI | ATTAAT | 1 cut(s) | 1079 |
| PsiI | TTATAA | 1 cut(s) | 560 |
| Psp124BI | GAGCTC | 1 cut(s) | 778 |
| Psp1406I | AACGTT | 1 cut(s) | 533 |
| Psp5II | RGGWCCY | 1 cut(s) | 79 |
| Psp6I | CCWGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| PspFI | CCCAGC | 1 cut(s) | 425 |
| PspGI | CCWGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| PspN4I | GGNNCC | 2 cut(s) | 264, 1032 |
| PspPI | GGNCC | 4 cut(s) | 79, 940, 1031, 1090 |
| PspPPI | RGGWCCY | 1 cut(s) | 79 |
| PstNI | CAGNNNCTG | 2 cut(s) | 503, 1390 |
| PsuI | RGATCY | 2 cut(s) | 262, 643 |
| RsaI | GTAC | 5 cut(s) | 355, 1001, 1127, 1133, 1265 |
| RsaNI | GTAC | 5 cut(s) | 354, 1000, 1126, 1132, 1264 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SacI | GAGCTC | 1 cut(s) | 778 |
| SaqAI | TTAA | 4 cut(s) | 240, 861, 1023, 1079 |
| SatI | GCNGC | 5 cut(s) | 198, 597, 684, 792, 1414 |
| Sau3AI | GATC | 9 cut(s) | 102, 174, 190, 262, 436, 553, 643, 691, 1116 |
| Sau96I | GGNCC | 4 cut(s) | 79, 940, 1031, 1090 |
| SchI | GAGTC | 4 cut(s) | 503, 811, 1136, 1296 |
| ScrFI | CCNGG | 5 cut(s) | 267, 1043, 1062, 1109, 1232 |
| SduI | GDGCHC | 4 cut(s) | 40, 778, 834, 1336 |
| SfaNI | GCATC | 2 cut(s) | 36, 1377 |
| SinI | GGWCC | 3 cut(s) | 79, 940, 1031 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SmlI | CTYRAG | 2 cut(s) | 699, 1304 |
| SmoI | CTYRAG | 2 cut(s) | 699, 1304 |
| Sse9I | AATT | 7 cut(s) | 14, 134, 510, 547, 561, 1035, 1076 |
| SsiI | CCGC | 6 cut(s) | 148, 198, 515, 1123, 1382, 1413 |
| SspI | AATATT | 1 cut(s) | 813 |
| SspMI | CTAG | 1 cut(s) | 401 |
| SstI | GAGCTC | 1 cut(s) | 778 |
| StyD4I | CCNGG | 5 cut(s) | 265, 1041, 1060, 1107, 1230 |
| TaaI | ACNGT | 1 cut(s) | 64 |
| TaiI | ACGT | 3 cut(s) | 353, 536, 1201 |
| TaqI | TCGA | 3 cut(s) | 69, 186, 421 |
| TasI | AATT | 7 cut(s) | 14, 134, 510, 547, 561, 1035, 1076 |
| TatI | WGTACW | 3 cut(s) | 353, 1131, 1263 |
| TauI | GCSGC | 2 cut(s) | 200, 1416 |
| TfiI | GAWTC | 4 cut(s) | 469, 919, 1193, 1309 |
| Tru1I | TTAA | 4 cut(s) | 240, 861, 1023, 1079 |
| Tru9I | TTAA | 4 cut(s) | 240, 861, 1023, 1079 |
| TscAI | CASTG | 1 cut(s) | 580 |
| TseFI | GTSAC | 3 cut(s) | 203, 581, 1322 |
| TseI | GCWGC | 3 cut(s) | 596, 683, 791 |
| Tsp45I | GTSAC | 3 cut(s) | 203, 581, 1322 |
| TspDTI | ATGAA | 9 cut(s) | 258, 911, 939, 1170, 1202, 1206, 1310, 1364, 1445 |
| TspRI | CASTG | 1 cut(s) | 580 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 479, 1284 |
| VneI | GTGCAC | 1 cut(s) | 1332 |
| VpaK11BI | GGWCC | 3 cut(s) | 79, 940, 1031 |
| VspI | ATTAAT | 1 cut(s) | 1079 |
| XceI | RCATGY | 1 cut(s) | 1132 |
| XmnI | GAANNNNTTC | 1 cut(s) | 930 |
| XspI | CTAG | 1 cut(s) | 401 |
| Zsp2I | ATGCAT | 1 cut(s) | 367 |