AT1G49170

DUF167

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011998
Physical Location & Seq
Reverse (-)
18183184 .. 18184583
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49170.1

Sequence Viewer

Length: 381 bp
ATGGCTCCGACGAAGAAAGGAAAGAAGACCAAATCGGCGGCGGAATCAACGACGGTCACAGAATCTTCAAGCTTTCCGACGTGTTTGCGTCTCCTTACACCTTCCTCCGTCGCTATCACCATCCATGCGAAGCCAGGTTCCAAAGCCGCCTCCATCACCGATGTGAGCGACGAAGCGGTAGGCGTTCAAATCGACGCGCCGGCTAGAGATGGTGAAGCTAACGCAGCTCTTCTCGAGTACATGAGCTCTGTATTGGGGGTGAAAAGAAGACAAGTCTCTCTCGGTTCGGGATCGAAGTCTCGAGATAAAGTTGTGATTGTTGAGGATATGACCCAACAGAGTGTTTTTCAAGCTTTGTCCCAAGCATCAAAACCTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF167

Protein Analysis

126

Amino Acids

13.12

Weight (kDa)

9.37

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF167 PF02594 37 - 108 1.1e-22 Uncharacterised ACR, YggU family COG1872
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 197
AciI CCGC 4 cut(s) 38, 41, 147, 176
AclWI GGATC 1 cut(s) 298
AfaI GTAC 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 3 cut(s) 69, 188, 350
AjiI CACGTC 1 cut(s) 81
AjnI CCWGG 1 cut(s) 133
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 5 cut(s) 72, 218, 227, 246, 353
AluI AGCT 5 cut(s) 72, 218, 227, 246, 353
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 3 cut(s) 95, 280, 303
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 2 cut(s) 233, 300
ApeKI GCWGC 1 cut(s) 224
AspLEI GCGC 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 109, 148, 224, 271
AvaI CYCGRG 2 cut(s) 233, 300
BanII GRGCYC 1 cut(s) 248
BbsI GAAGAC 2 cut(s) 32, 274
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 236
BccI CCATC 3 cut(s) 128, 161, 203
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 95, 280, 303
BfaI CTAG 1 cut(s) 204
BisI GCNGC 3 cut(s) 39, 147, 225
BlsI GCNGC 3 cut(s) 40, 148, 226
Bme1390I CCNGG 1 cut(s) 135
BmeT110I CYCGRG 2 cut(s) 233, 300
BmgBI CACGTC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 6, 139
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 32, 274
Bse118I RCCGGY 1 cut(s) 199
BseBI CCWGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 2 cut(s) 233, 300
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 343
BsmAI GTCTC 3 cut(s) 95, 280, 303
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 343
BsoBI CYCGRG 2 cut(s) 233, 300
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 1 cut(s) 290
BspACI CCGC 4 cut(s) 38, 41, 147, 176
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 6, 139
BspPI GGATC 1 cut(s) 298
BspQI GCTCTTC 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssMI GATC 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 201
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 1 cut(s) 293
BstMAI GTCTC 3 cut(s) 95, 280, 303
BstMBI GATC 1 cut(s) 290
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 2 cut(s) 32, 274
BtrI CACGTC 1 cut(s) 81
BtsCI GGATG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 201
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 199
CseI GACGC 2 cut(s) 77, 203
Csp6I GTAC 1 cut(s) 238
CviAII CATG 2 cut(s) 125, 241
CviJI RGCY 9 cut(s) 5, 72, 133, 146, 203, 218, 227, 246, 353
CviKI_1 RGCY 9 cut(s) 5, 72, 133, 146, 203, 218, 227, 246, 353
CviQI GTAC 1 cut(s) 238
DpnI GATC 1 cut(s) 292
DpnII GATC 1 cut(s) 290
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 246
Eco24I GRGCYC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 246
Eco88I CYCGRG 2 cut(s) 233, 300
EcoICRI GAGCTC 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 2 cut(s) 128, 244
FaiI YATR 3 cut(s) 126, 242, 329
FaqI GGGAC 1 cut(s) 343
FatI CATG 2 cut(s) 124, 240
Fnu4HI GCNGC 3 cut(s) 39, 147, 225
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 3 cut(s) 39, 147, 225
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 198
GluI GCNGC 3 cut(s) 39, 147, 225
HapII CCGG 1 cut(s) 200
HgaI GACGC 2 cut(s) 77, 203
HhaI GCGC 1 cut(s) 199
Hin1II CATG 2 cut(s) 128, 244
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HindIII AAGCTT 2 cut(s) 70, 351
HinfI GANTC 2 cut(s) 44, 62
HpaII CCGG 1 cut(s) 200
HphI GGTGA 4 cut(s) 109, 148, 224, 271
Hpy188I TCNGA 2 cut(s) 9, 78
Hpy188III TCNNGA 4 cut(s) 233, 288, 300, 302
Hpy99I CGWCG 6 cut(s) 13, 55, 82, 113, 173, 197
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 80
Hsp92II CATG 2 cut(s) 128, 244
HspAI GCGC 1 cut(s) 197
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 1 cut(s) 290
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 3 cut(s) 120, 147, 213
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 374
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 292
MboI GATC 1 cut(s) 290
MboII GAAGA 5 cut(s) 25, 37, 57, 221, 279
MhlI GDGCHC 1 cut(s) 248
MmeI TCCRAC 2 cut(s) 32, 101
MnlI CCTC 3 cut(s) 115, 160, 316
MroNI GCCGGC 1 cut(s) 199
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 224
NaeI GCCGGC 1 cut(s) 201
NdeII GATC 1 cut(s) 290
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 2 cut(s) 128, 244
NlaIV GGNNCC 2 cut(s) 6, 139
NmuCI GTSAC 1 cut(s) 55
OliI CACNNNNGTG 1 cut(s) 161
PaeR7I CTCGAG 2 cut(s) 233, 300
PciSI GCTCTTC 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 2 cut(s) 44, 62
PkrI GCNGC 3 cut(s) 40, 148, 226
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 6, 139
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 161
SacI GAGCTC 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 234
SatI GCNGC 3 cut(s) 39, 147, 225
Sau3AI GATC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 1 cut(s) 374
Sfr274I CTCGAG 2 cut(s) 233, 300
SlaI CTCGAG 2 cut(s) 233, 300
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 2 cut(s) 233, 300
SmoI CTYRAG 2 cut(s) 233, 300
SsiI CCGC 4 cut(s) 38, 41, 147, 176
SspMI CTAG 1 cut(s) 204
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 83
TaqI TCGA 4 cut(s) 192, 234, 293, 301
TatI WGTACW 1 cut(s) 237
TauI GCSGC 2 cut(s) 41, 149
TfiI GAWTC 2 cut(s) 44, 62
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 55
TspGWI ACGGA 1 cut(s) 97
XhoI CTCGAG 2 cut(s) 233, 300
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.