FvH4_1g23360

UPF0235 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011998
Physical Location & Seq
Forward (+)
15210941 .. 15214019
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23360.t1

Sequence Viewer

Length: 441 bp
ATGACCGACCCGGTTATAAACAAGACTAAAGAAGGCAGAGAAGAGGGACAGGGAGTCTCAATGGCTCCAGCCAAGAAGCGAAAGGCCAAAGCCAAACCCAACGCTCCCGCCGAGTCGACTCAGTCAAATTTCCCGGCGTGTATTCGCTCCGTACCTCCCTCCTCAGTCGCCATCACCATCCACGCCAAACCCGGCTCCAAACTCGCCTCCATCACCGATTTCAGCGACGAGGCATTGGGTGTGCAGATCGACGCGCCCGCCAAGGACGGCGAAGCTAACGCCGCCCTTCTCGACTTCATGAGTTCCGTGTTGGGTGTAAAAAGAAGGCAGGTTTCTATTGGATGTGGTTCTAAATCAAGAGACAAGGTTGTTATTGTAGAGGAGATGACTTTACAAAGTGTTTTTGACATTCTGGATAAAGCTTCAAAGTGCAGCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF167

Protein Analysis

147

Amino Acids

15.36

Weight (kDa)

9.03

Isoelectric Point (pI)

59.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF167 PF02594 56 - 127 5e-21 Uncharacterised ACR, YggU family COG1872
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 116
AccII CGCG 1 cut(s) 254
AciI CCGC 3 cut(s) 108, 258, 282
AcsI RAATTY 1 cut(s) 127
AfaI GTAC 1 cut(s) 153
AgsI TTSAA 1 cut(s) 426
AhdI GACNNNNNGTC 1 cut(s) 53
AluBI AGCT 2 cut(s) 275, 422
AluI AGCT 2 cut(s) 275, 422
Alw26I GTCTC 2 cut(s) 61, 354
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 432
ApoI RAATTY 1 cut(s) 127
AspLEI GCGC 2 cut(s) 256, 437
AsuC2I CCSGG 3 cut(s) 11, 134, 192
AsuHPI GGTGA 2 cut(s) 166, 205
BccI CCATC 3 cut(s) 179, 185, 218
BceAI ACGGC 1 cut(s) 283
BcnI CCSGG 3 cut(s) 11, 134, 192
BcoDI GTCTC 2 cut(s) 61, 354
BfuAI ACCTGC 1 cut(s) 319
BisI GCNGC 2 cut(s) 282, 433
BlsI GCNGC 2 cut(s) 283, 434
Bme1390I CCNGG 3 cut(s) 11, 134, 192
BmeRI GACNNNNNGTC 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 66, 196
BmrFI CCNGG 3 cut(s) 11, 134, 192
BpmI CTGGAG 1 cut(s) 51
BpuMI CCSGG 3 cut(s) 11, 134, 192
BsaJI CCNNGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 2 cut(s) 177, 347
BseMII CTCAG 2 cut(s) 134, 177
BseRI GAGGAG 2 cut(s) 151, 395
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 86
BsiSI CCGG 3 cut(s) 11, 134, 192
BslFI GGGAC 1 cut(s) 60
BsmAI GTCTC 2 cut(s) 61, 354
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 3 cut(s) 108, 258, 282
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 133, 176
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 66, 196
BspMI ACCTGC 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 261
Bst6I CTCTTC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 120, 163
BstF5I GGATG 2 cut(s) 177, 347
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 256, 437
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 2 cut(s) 61, 354
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 9, 132, 190
BstUI CGCG 1 cut(s) 254
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 2 cut(s) 177, 347
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 258
CciI TCATGA 1 cut(s) 297
CfoI GCGC 2 cut(s) 256, 437
CseI GACGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 152
CviAII CATG 1 cut(s) 298
CviJI RGCY 7 cut(s) 65, 71, 86, 92, 195, 275, 422
CviKI_1 RGCY 7 cut(s) 65, 71, 86, 92, 195, 275, 422
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 2 cut(s) 120, 163
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DriI GACNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 1 cut(s) 301
FaiI YATR 3 cut(s) 17, 299, 439
FaqI GGGAC 1 cut(s) 60
FatI CATG 1 cut(s) 297
FauI CCCGC 2 cut(s) 115, 265
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 2 cut(s) 282, 433
FokI GGATG 2 cut(s) 164, 354
Fsp4HI GCNGC 2 cut(s) 282, 433
GlaI GCGC 2 cut(s) 255, 436
GluI GCNGC 2 cut(s) 282, 433
GsuI CTGGAG 1 cut(s) 51
HaeIII GGCC 1 cut(s) 86
HapII CCGG 3 cut(s) 11, 134, 192
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 256, 437
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 2 cut(s) 254, 435
HinP1I GCGC 2 cut(s) 254, 435
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 3 cut(s) 54, 113, 118
HpaII CCGG 3 cut(s) 11, 134, 192
HphI GGTGA 2 cut(s) 166, 205
Hpy166II GTNNAC 1 cut(s) 117
Hpy188III TCNNGA 4 cut(s) 290, 298, 357, 413
Hpy8I GTNNAC 1 cut(s) 117
Hpy99I CGWCG 2 cut(s) 230, 254
HpyAV CCTTC 3 cut(s) 26, 296, 318
HpyCH4V TGCA 2 cut(s) 244, 432
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 2 cut(s) 120, 163
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 2 cut(s) 254, 435
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 4 cut(s) 70, 109, 152, 200
LpnPI CCDG 7 cut(s) 24, 35, 81, 147, 205, 314, 398
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 53
MluCI AATT 1 cut(s) 127
MlyI GAGTC 3 cut(s) 63, 112, 122
MnlI CCTC 7 cut(s) 37, 165, 169, 172, 217, 223, 373
MspI CCGG 3 cut(s) 11, 134, 192
MspR9I CCNGG 3 cut(s) 11, 134, 192
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 281
NciI CCSGG 3 cut(s) 11, 134, 192
NdeII GATC 1 cut(s) 246
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 66, 196
NmeAIII GCCGAG 1 cut(s) 136
PagI TCATGA 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 121
PkrI GCNGC 2 cut(s) 283, 434
PleI GAGTC 3 cut(s) 62, 112, 121
PpsI GAGTC 3 cut(s) 62, 112, 121
PsiI TTATAA 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 66, 196
PsyI GACNNNGTC 1 cut(s) 121
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
SalI GTCGAC 1 cut(s) 115
SatI GCNGC 2 cut(s) 282, 433
Sau3AI GATC 1 cut(s) 246
SchI GAGTC 3 cut(s) 63, 112, 122
ScrFI CCNGG 3 cut(s) 11, 134, 192
SetI ASST 5 cut(s) 157, 277, 333, 369, 424
Sse9I AATT 1 cut(s) 127
SsiI CCGC 3 cut(s) 108, 258, 282
StyD4I CCNGG 3 cut(s) 9, 132, 190
StyI CCWWGG 1 cut(s) 261
TaqI TCGA 3 cut(s) 116, 249, 291
TaqII GACCGA 1 cut(s) 20
TasI AATT 1 cut(s) 127
TauI GCSGC 1 cut(s) 284
TseI GCWGC 1 cut(s) 432
TspDTI ATGAA 1 cut(s) 286
TspGWI ACGGA 2 cut(s) 139, 295
Tth111I GACNNNGTC 1 cut(s) 121
XapI RAATTY 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.