MD05G1015900.v1.1

UPF0235 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011998
Physical Location & Seq
Forward (+)
2863646 .. 2864038
393 bp
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UTR
Exon/CDS
Intron
MD05G1015900.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCACCGGGCAAGAAAGGCAAGGCCAAGGCGAAGTCGGCGAGCCCTGCGGAGCCAACTCAGTCCGACAACAACAACTTCCCGGCCTGTATTCGGTCGGTACCTCCTTCGTCGGTGGCCATCACCATCCACGCCAAGCCCGGCTCCAAAATCGCCGCCATCACGGATTTCAGCGACGATGCGTTGGGGGTGCAAATCGACGCGCCCCCGAAGGACGGGGAGGCGAATGCTGCACTGGTCGAGTACATGAGCTCGGTTTTAGGGGTAAAAAGACGGCAGGTTTCGATCGGGTCGGGCTCCAAATCAAGAGACAAGGTTGTTATTGTGGAGGACGTGACCCTTCAAAAGGTTTTCGACACTCTCGACAAAGCTGCTGCTTCCAAAAACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF167

Protein Analysis

131

Amino Acids

13.48

Weight (kDa)

9.27

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF167 PF02594 39 - 110 1.8e-23 Uncharacterised ACR, YggU family COG1872
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
Acc65I GGTACC 1 cut(s) 100
AccB1I GGYRCC 2 cut(s) 4, 100
AccII CGCG 1 cut(s) 203
AciI CCGC 2 cut(s) 50, 156
AcoI YGGCCR 1 cut(s) 117
AfaI GTAC 2 cut(s) 102, 245
AfiI CCNNNNNNNGG 3 cut(s) 93, 215, 346
AgsI TTSAA 1 cut(s) 344
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 2 cut(s) 252, 371
AluI AGCT 2 cut(s) 252, 371
Alw21I GWGCWC 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 303
AoxI GGCC 3 cut(s) 24, 84, 117
ApeKI GCWGC 3 cut(s) 230, 371, 374
Asp718I GGTACC 1 cut(s) 100
AspLEI GCGC 1 cut(s) 205
AsuC2I CCSGG 3 cut(s) 9, 83, 141
AsuHPI GGTGA 1 cut(s) 115
BalI TGGCCA 1 cut(s) 119
BanI GGYRCC 2 cut(s) 4, 100
BanII GRGCYC 3 cut(s) 47, 254, 299
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 3 cut(s) 217, 358, 361
BccI CCATC 3 cut(s) 128, 134, 167
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BcnI CCSGG 3 cut(s) 9, 83, 141
BcoDI GTCTC 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 156, 231, 372, 375
BlsI GCNGC 4 cut(s) 157, 232, 373, 376
Bme1390I CCNGG 3 cut(s) 9, 83, 141
BmgBI CACGTC 1 cut(s) 334
BmiI GGNNCC 5 cut(s) 6, 54, 102, 145, 298
BmrFI CCNGG 3 cut(s) 9, 83, 141
BmsI GCATC 1 cut(s) 169
BpuMI CCSGG 3 cut(s) 9, 83, 141
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 215, 346
Bse1I ACTGG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 3 cut(s) 93, 215, 346
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 217, 358, 361
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 2 cut(s) 98, 288
BshFI GGCC 3 cut(s) 26, 86, 119
BshNI GGYRCC 2 cut(s) 4, 100
BsiEI CGRYCG 2 cut(s) 98, 288
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 3 cut(s) 8, 83, 141
BslI CCNNNNNNNGG 3 cut(s) 93, 215, 346
BsmAI GTCTC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 3 cut(s) 26, 86, 119
Bsp1286I GDGCHC 3 cut(s) 47, 254, 299
Bsp143I GATC 1 cut(s) 285
BspACI CCGC 2 cut(s) 50, 156
BspANI GGCC 3 cut(s) 26, 86, 119
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 5 cut(s) 6, 54, 102, 145, 298
BspMI ACCTGC 1 cut(s) 268
BspT107I GGYRCC 2 cut(s) 4, 100
BsrI ACTGG 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 285
BssT1I CCWWGG 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 60
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 1 cut(s) 285
BstMCI CGRYCG 2 cut(s) 98, 288
BstMWI GCNNNNNNNGC 4 cut(s) 18, 38, 47, 230
BstSCI CCNGG 3 cut(s) 7, 81, 139
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 217, 358, 361
BsuRI GGCC 3 cut(s) 26, 86, 119
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 233
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 205
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 101, 244
CviAII CATG 1 cut(s) 247
CviQI GTAC 2 cut(s) 101, 244
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 3 cut(s) 47, 254, 299
Eco53kI GAGCTC 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 252
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 3 cut(s) 47, 254, 299
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 1 cut(s) 250
FaiI YATR 1 cut(s) 248
FatI CATG 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 156, 231, 372, 375
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 3 cut(s) 47, 254, 299
Fsp4HI GCNGC 4 cut(s) 156, 231, 372, 375
GlaI GCGC 1 cut(s) 204
GluI GCNGC 4 cut(s) 156, 231, 372, 375
HaeIII GGCC 3 cut(s) 26, 86, 119
HapII CCGG 3 cut(s) 8, 83, 141
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 205
Hin1II CATG 1 cut(s) 250
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HpaII CCGG 3 cut(s) 8, 83, 141
HphI GGTGA 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 2 cut(s) 306, 362
Hpy99I CGWCG 3 cut(s) 115, 179, 203
HpyAV CCTTC 3 cut(s) 117, 205, 350
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 2 cut(s) 193, 233
HpyF10VI GCNNNNNNNGC 4 cut(s) 18, 38, 47, 230
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 1 cut(s) 250
HspAI GCGC 1 cut(s) 203
KpnI GGTACC 1 cut(s) 104
Kzo9I GATC 1 cut(s) 285
LmnI GCTCC 3 cut(s) 52, 149, 302
LpnPI CCDG 7 cut(s) 21, 60, 96, 100, 154, 221, 263
Lsp1109I GCAGC 3 cut(s) 217, 358, 361
LweI GCATC 1 cut(s) 169
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 334
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MhlI GDGCHC 3 cut(s) 47, 254, 299
MlsI TGGCCA 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 3 cut(s) 114, 214, 322
Mox20I TGGCCA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 119
MspI CCGG 3 cut(s) 8, 83, 141
MspR9I CCNGG 3 cut(s) 9, 83, 141
Mva1269I GAATGC 1 cut(s) 232
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 18, 38, 47, 230
NciI CCSGG 3 cut(s) 9, 83, 141
NdeII GATC 1 cut(s) 285
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 5 cut(s) 6, 54, 102, 145, 298
NmuCI GTSAC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 232
PkrI GCNGC 4 cut(s) 157, 232, 373, 376
Ple19I CGATCG 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 254
PspN4I GGNNCC 5 cut(s) 6, 54, 102, 145, 298
PvuI CGATCG 1 cut(s) 288
RsaI GTAC 2 cut(s) 102, 245
RsaNI GTAC 2 cut(s) 101, 244
SacI GAGCTC 1 cut(s) 254
SatI GCNGC 4 cut(s) 156, 231, 372, 375
Sau3AI GATC 1 cut(s) 285
ScrFI CCNGG 3 cut(s) 9, 83, 141
SduI GDGCHC 3 cut(s) 47, 254, 299
SetI ASST 8 cut(s) 106, 254, 282, 318, 336, 351, 373, 392
SfaNI GCATC 1 cut(s) 169
SsiI CCGC 2 cut(s) 50, 156
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 3 cut(s) 7, 81, 139
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 336
TaqI TCGA 5 cut(s) 198, 240, 284, 354, 363
TaqII GACCGA 1 cut(s) 84
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 158
TscAI CASTG 1 cut(s) 240
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 230, 371, 374
Tsp45I GTSAC 1 cut(s) 334
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 1 cut(s) 240
XagI CCTNNNNNAGG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.