AT1G49910

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012239
Physical Location & Seq
Forward (+)
18478951 .. 18481539
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49910.1

Sequence Viewer

Length: 1020 bp
ATGACTTTGGTGCCGGCCATTGGTCGCGAGCTCTCGAATCCACCGTCCGATGGGATTTCTAATCTTCGATTTTCTAATAACAGCGATCATTTACTAGTCTCTTCATGGGATAAGAGTGTAAGATTGTATGATGCGAACGGCGATTTGATGAGAGGGGAGTTTAAACATGGTGGAGCGGTACTCGATTGCTGCTTCCATGATGATTCTTCTGGATTCAGTGTTTGCGCCGATACTAAAGTTAGAAGAATTGACTTCAATGCTGGCAAAGAAGACGTTTTGGGTACGCATGAGAAGCCAGTTCGATGTGTTGAGTATTCTTATGCTGCAGGGCAAGTGATCACTGGAAGTTGGGATAAAACGATTAAATGTTGGGATCCAAGAGGTGCAAGTGGGACTGAGCGCACACAGATTGGAACTTATATGCAACCTGAGCGTGTTAACTCTCTTTCTCTTGTTGGAAATCGTTTGGTAGTGGCAACAGCAGGAAGGCATGTCAACATTTATGATCTTAGAAATATGTCCCAGCCTGAGCAAAGAAGAGAGTCCTCACTTAAATACCAGACAAGATGTGTACGTTGTTATCCCAACGGAACAGGATATGCCCTTAGCTCTGTTGAAGGGAGGGTTTCAATGGAGTTTTTTGATCTATCAGAAGCTGCTCAGGCTAAAAAATATGCTTTCAAATGTCACCGGAAATCAGAGGATGGAAGGGACATTGTCTACCCTGTAAATGCAATTGCTTTCCATCCGATTTATGGCACTTTTGCTTCCGGAGGCTGTGATGGTTTTGTCAACATTTGGGACGGTAACAATAAGAAGAGGCTTTATCAGTACTCTAAGTATCCAACAAGTATTGCGGCGCTGTCATTCAGCCGAGATGGTGGATTACTGGCTGTTGCTTCTAGTTACACGTTTGAAGAGGGAGACAAACCGCATGAACCGGACGCCATCTTTGTTAGAAGTGTTAATGAAATTGAAGTGAAACCGAAACCCAAAGTATACCCAAATCCCCCGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

339

Amino Acids

37.67

Weight (kDa)

8.0

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 16 - 125 2.7e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 17 - 226 1.6e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 18 - 125 7.6e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 61 - 223 2.8e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 73 - 302 5e-23 THOC3 beta-propeller domain
WD40 PF00400 92 - 125 2.1e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 144 - 284 4.6e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 147 - 298 2.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 155 - 302 4.6e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 240 - 302 5.1e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 1 cut(s) 176
AccI GTMKAC 2 cut(s) 720, 999
AccII CGCG 1 cut(s) 27
AccIII TCCGGA 1 cut(s) 770
AciI CCGC 3 cut(s) 176, 857, 932
AclWI GGATC 2 cut(s) 368, 381
AcoI YGGCCR 1 cut(s) 15
AcyI GRCGYC 1 cut(s) 945
AfaI GTAC 4 cut(s) 180, 283, 573, 833
AfiI CCNNNNNNNGG 3 cut(s) 20, 50, 755
AflIII ACRYGT 1 cut(s) 909
AgsI TTSAA 6 cut(s) 256, 617, 630, 682, 917, 977
AhlI ACTAGT 1 cut(s) 94
AluBI AGCT 3 cut(s) 31, 609, 656
AluI AGCT 3 cut(s) 31, 609, 656
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 103, 918
AlwI GGATC 2 cut(s) 368, 381
AlwNI CAGNNNCTG 1 cut(s) 656
Aor13HI TCCGGA 1 cut(s) 770
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 3 cut(s) 189, 323, 656
ArsI GACNNNNNNTTYG 4 cut(s) 29, 61, 935, 967
AspLEI GCGC 3 cut(s) 227, 402, 862
AsuC2I CCSGG 1 cut(s) 1013
AsuHPI GGTGA 1 cut(s) 680
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BamHI GGATCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 3 cut(s) 176, 310, 643
BccI CCATC 6 cut(s) 44, 698, 753, 776, 872, 956
BceAI ACGGC 1 cut(s) 154
BciVI GTATCC 1 cut(s) 852
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 1013
BcoDI GTCTC 2 cut(s) 103, 918
BcuI ACTAGT 1 cut(s) 94
BfaI CTAG 2 cut(s) 95, 903
BfmI CTRYAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 863
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 4 cut(s) 190, 324, 657, 858
BlsI GCNGC 4 cut(s) 191, 325, 658, 859
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 1 cut(s) 1013
BmiI GGNNCC 2 cut(s) 12, 375
BmrFI CCNGG 1 cut(s) 1013
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 165, 197
Bpu10I CCTNAGC 4 cut(s) 429, 528, 605, 660
BpuMI CCSGG 1 cut(s) 1013
BsaHI GRCGYC 1 cut(s) 945
BsaJI CCNNGG 1 cut(s) 1011
BsaWI WCCGGW 3 cut(s) 690, 770, 940
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 50, 755
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 296, 346, 894
BseAI TCCGGA 1 cut(s) 770
BseDI CCNNGG 1 cut(s) 1011
BseGI GGATG 2 cut(s) 709, 745
BseLI CCNNNNNNNGG 3 cut(s) 20, 50, 755
BseMII CTCAG 4 cut(s) 387, 420, 519, 674
BseNI ACTGG 3 cut(s) 296, 346, 894
BseXI GCAGC 3 cut(s) 176, 310, 643
BseYI CCCAGC 1 cut(s) 522
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 10
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 5 cut(s) 14, 691, 771, 941, 1013
BslFI GGGAC 4 cut(s) 406, 505, 725, 815
BslI CCNNNNNNNGG 3 cut(s) 20, 50, 755
BsmAI GTCTC 2 cut(s) 103, 918
BsmFI GGGAC 4 cut(s) 406, 505, 725, 815
BsnI GGCC 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 33
Bsp13I TCCGGA 1 cut(s) 770
Bsp143I GATC 5 cut(s) 85, 336, 373, 505, 643
Bsp68I TCGCGA 1 cut(s) 27
BspACI CCGC 3 cut(s) 176, 857, 932
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 4 cut(s) 388, 421, 520, 673
BspEI TCCGGA 1 cut(s) 770
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 12, 375
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 2 cut(s) 368, 381
BspT107I GGYRCC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 296, 346, 894
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 1011
BssMI GATC 5 cut(s) 85, 336, 373, 505, 643
BssNAI GTATAC 1 cut(s) 1000
BssNI GRCGYC 1 cut(s) 945
Bst1107I GTATAC 1 cut(s) 1000
Bst4CI ACNGT 2 cut(s) 45, 806
Bst6I CTCTTC 4 cut(s) 106, 532, 812, 912
BstACI GRCGYC 1 cut(s) 945
BstC8I GCNNGC 3 cut(s) 15, 29, 262
BstDEI CTNAG 7 cut(s) 396, 429, 509, 528, 605, 660, 837
BstF5I GGATG 2 cut(s) 709, 745
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 863
BstHHI GCGC 3 cut(s) 227, 402, 862
BstKTI GATC 5 cut(s) 88, 339, 376, 508, 646
BstMAI GTCTC 2 cut(s) 103, 918
BstMBI GATC 5 cut(s) 85, 336, 373, 505, 643
BstMWI GCNNNNNNNGC 3 cut(s) 292, 430, 662
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 1011
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 176, 310, 643
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BstZ17I GTATAC 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 2 cut(s) 709, 745
BtsIMutI CAGTG 2 cut(s) 223, 339
BtuMI TCGCGA 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 15, 29, 262
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 3 cut(s) 227, 402, 862
Cfr10I RCCGGY 1 cut(s) 13
CseI GACGC 1 cut(s) 953
Csp6I GTAC 4 cut(s) 179, 282, 572, 832
CviAII CATG 6 cut(s) 105, 167, 197, 287, 491, 935
CviQI GTAC 4 cut(s) 179, 282, 572, 832
DdeI CTNAG 7 cut(s) 396, 429, 509, 528, 605, 660, 837
DpnI GATC 5 cut(s) 87, 338, 375, 507, 645
DpnII GATC 5 cut(s) 85, 336, 373, 505, 643
DraI TTTAAA 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 4 cut(s) 106, 532, 812, 912
EarI CTCTTC 4 cut(s) 106, 532, 812, 912
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 33
FaeI CATG 6 cut(s) 108, 170, 200, 290, 494, 938
FaqI GGGAC 4 cut(s) 406, 505, 725, 815
FatI CATG 6 cut(s) 104, 166, 196, 286, 490, 934
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 720, 999
Fnu4HI GCNGC 4 cut(s) 190, 324, 657, 858
FokI GGATG 2 cut(s) 716, 732
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 4 cut(s) 190, 324, 657, 858
FspBI CTAG 2 cut(s) 95, 903
GlaI GCGC 3 cut(s) 226, 401, 861
GluI GCNGC 4 cut(s) 190, 324, 657, 858
GsaI CCCAGC 1 cut(s) 526
HaeII RGCGCY 1 cut(s) 863
HaeIII GGCC 1 cut(s) 17
HapII CCGG 5 cut(s) 14, 691, 771, 941, 1013
HgaI GACGC 1 cut(s) 953
HhaI GCGC 3 cut(s) 227, 402, 862
Hin1I GRCGYC 1 cut(s) 945
Hin1II CATG 6 cut(s) 108, 170, 200, 290, 494, 938
Hin6I GCGC 3 cut(s) 225, 400, 860
HinP1I GCGC 3 cut(s) 225, 400, 860
HincII GTYRAC 3 cut(s) 439, 496, 793
HindII GTYRAC 3 cut(s) 439, 496, 793
HinfI GANTC 4 cut(s) 37, 203, 213, 542
HpaI GTTAAC 1 cut(s) 439
HpaII CCGG 5 cut(s) 14, 691, 771, 941, 1013
HphI GGTGA 1 cut(s) 680
Hpy166II GTNNAC 6 cut(s) 439, 496, 572, 721, 793, 1000
Hpy188I TCNGA 4 cut(s) 49, 652, 700, 750
Hpy188III TCNNGA 4 cut(s) 26, 34, 210, 771
Hpy8I GTNNAC 6 cut(s) 439, 496, 572, 721, 793, 1000
HpyAV CCTTC 3 cut(s) 480, 611, 702
HpyCH4III ACNGT 2 cut(s) 45, 806
HpyCH4IV ACGT 3 cut(s) 273, 574, 911
HpyCH4V TGCA 4 cut(s) 326, 386, 424, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 430, 662
HpyF3I CTNAG 7 cut(s) 396, 429, 509, 528, 605, 660, 837
HpySE526I ACGT 3 cut(s) 273, 574, 911
Hsp92I GRCGYC 1 cut(s) 945
Hsp92II CATG 6 cut(s) 108, 170, 200, 290, 494, 938
HspAI GCGC 3 cut(s) 225, 400, 860
Kpn2I TCCGGA 1 cut(s) 770
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 336
KspAI GTTAAC 1 cut(s) 439
Kzo9I GATC 5 cut(s) 85, 336, 373, 505, 643
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 3 cut(s) 176, 310, 643
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 95, 903
MaeII ACGT 3 cut(s) 273, 574, 911
MaeIII GTNAC 3 cut(s) 686, 806, 905
MalI GATC 5 cut(s) 87, 338, 375, 507, 645
MbiI CCGCTC 1 cut(s) 176
MboI GATC 5 cut(s) 85, 336, 373, 505, 643
MboII GAAGA 8 cut(s) 56, 93, 198, 255, 281, 549, 829, 929
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 246, 735, 972
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 436, 869
MnlI CCTC 8 cut(s) 146, 374, 556, 615, 694, 767, 813, 913
MroI TCCGGA 1 cut(s) 770
MroNI GCCGGC 1 cut(s) 13
MseI TTAA 5 cut(s) 162, 363, 438, 552, 966
MspI CCGG 5 cut(s) 14, 691, 771, 941, 1013
MspR9I CCNGG 1 cut(s) 1013
MssI GTTTAAAC 1 cut(s) 163
MunI CAATTG 1 cut(s) 735
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 292, 430, 662
NaeI GCCGGC 1 cut(s) 15
NciI CCSGG 1 cut(s) 1013
NdeII GATC 5 cut(s) 85, 336, 373, 505, 643
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 6 cut(s) 108, 170, 200, 290, 494, 938
NlaIV GGNNCC 2 cut(s) 12, 375
NmeAIII GCCGAG 1 cut(s) 899
NmuCI GTSAC 1 cut(s) 686
NruI TCGCGA 1 cut(s) 27
NspI RCATGY 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 15
PfeI GAWTC 3 cut(s) 37, 203, 213
PflFI GACNNNGTC 1 cut(s) 716
PkrI GCNGC 4 cut(s) 191, 325, 658, 859
PleI GAGTC 1 cut(s) 550
PmeI GTTTAAAC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 550
Psp124BI GAGCTC 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 522
PspN4I GGNNCC 2 cut(s) 12, 375
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 373
PsyI GACNNNGTC 1 cut(s) 716
RruI TCGCGA 1 cut(s) 27
RsaI GTAC 4 cut(s) 180, 283, 573, 833
RsaNI GTAC 4 cut(s) 179, 282, 572, 832
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 5 cut(s) 162, 363, 438, 552, 966
SatI GCNGC 4 cut(s) 190, 324, 657, 858
Sau3AI GATC 5 cut(s) 85, 336, 373, 505, 643
ScaI AGTACT 1 cut(s) 833
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 1013
SduI GDGCHC 1 cut(s) 33
SetI ASST 8 cut(s) 33, 276, 385, 430, 577, 611, 658, 914
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 324
SpeI ACTAGT 1 cut(s) 94
Sse9I AATT 3 cut(s) 246, 735, 972
SsiI CCGC 3 cut(s) 176, 857, 932
SspMI CTAG 2 cut(s) 95, 903
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 1011
TaaI ACNGT 2 cut(s) 45, 806
TaiI ACGT 3 cut(s) 276, 577, 914
TaqI TCGA 4 cut(s) 35, 67, 183, 301
TasI AATT 3 cut(s) 246, 735, 972
TatI WGTACW 1 cut(s) 831
TauI GCSGC 1 cut(s) 860
TfiI GAWTC 3 cut(s) 37, 203, 213
Tru1I TTAA 5 cut(s) 162, 363, 438, 552, 966
Tru9I TTAA 5 cut(s) 162, 363, 438, 552, 966
TscAI CASTG 2 cut(s) 223, 346
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 3 cut(s) 189, 323, 656
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 3 cut(s) 93, 951, 984
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 2 cut(s) 223, 346
Tth111I GACNNNGTC 1 cut(s) 716
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XmiI GTMKAC 2 cut(s) 720, 999
XspI CTAG 2 cut(s) 95, 903
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.