AT3G19590

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012239
Physical Location & Seq
Forward (+)
6805622 .. 6808593
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19590.1

Sequence Viewer

Length: 1023 bp
ATGACGACTGTGACTCCGTCCGCCGGTCGTGAGCTCTCGAATCCGCCGTCCGACGGCATTTCTAATCTCCGATTTTCTAATAACAGTGATCATCTCCTCGTTTCTTCATGGGATAAGCGTGTGAGATTGTATGATGTGAGCACCAATTCGTTGAAAGGAGAGTTCTTACATGGCGGAGCAGTACTCGATTGCTGTTTTCACGATGACTTCTCCGGCTTCAGTGTTGGCGCTGATTACAAAGTCCGACGGATTGTATTCAATGTCGGCAAAGAGGACATTTTGGGGACACATGACAAAGCAGTGCGATGTGTTGAGTATTCTTACGCTGCGGGACAAGTGATCACTGGATCTTGGGATAAAACAGTTAAATGTTGGGATCCAAGAGGCGCTAGTGGGCCTGAACGCACCCAAGTGGGAACATATTTGCAACCAGAACGTGTTTACTCTATGTCTCTTGTTGGACATCGTTTGGTAGTGGCTACAGCAGGAAGGCATGTAAACATCTATGATCTCAGAAATATGTCTCAGCCTGAGCAAAGAAGGGAGTCTTCACTGAAATACCAGACGAGATGTGTGCGTTGTTATCCTAATGGAACAGGCTATGCTCTTAGCTCTGTTGAAGGAAGGGTTGCAATGGAGTTTTTTGATCTGTCAGAGGCTGCTCAAGCTAAAAAATATGCTTTCAAATGTCATCGGAAATCAGAGGCTGGAAGGGACATTGTTTACCCTGTAAATTCCATTGCCTTCCATCCAATCTATGGCACCTTTGCAACTGGAGGCTGTGATGGTTTCGTCAACATTTGGGATGGTAACAACAAGAAGAGGCTATATCAGTACTCAAAGTATCCAACGAGTATCTCGGCACTGTCATTCAGTCGAGATGGTCAGCTGCTGGCTGTTGCTTCAAGTTACACATTTGAAGAGGGAGAGAAATCGCAAGAACCGGAGGCCATCTTTGTAAGAAGCGTGAATGAAATCGAAGTGAAACCAAAACCGAAAGTATACCCGAATCCTGCGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

340

Amino Acids

37.87

Weight (kDa)

8.65

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 18 - 228 7.6e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 19 - 126 2.7e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 72 - 224 5.3e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 90 - 303 2.1e-23 THOC3 beta-propeller domain
WD40 PF00400 94 - 126 3.7e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 97 - 291 1.6e-10 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 154 - 299 5.4e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 241 - 303 2.3e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 244 - 302 3.9e-06 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 246 - 302 3e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 758
AccI GTMKAC 1 cut(s) 1002
AciI CCGC 5 cut(s) 21, 44, 174, 329, 1016
AclWI GGATC 3 cut(s) 355, 371, 384
AcsI RAATTY 1 cut(s) 733
AcuI CTGAAG 1 cut(s) 202
AfaI GTAC 2 cut(s) 183, 836
AfiI CCNNNNNNNGG 3 cut(s) 23, 53, 758
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 6 cut(s) 154, 259, 620, 685, 906, 920
AleI CACNNNNGTG 1 cut(s) 410
AluBI AGCT 4 cut(s) 34, 612, 668, 889
AluI AGCT 4 cut(s) 34, 612, 668, 889
Alw21I GWGCWC 2 cut(s) 36, 143
Alw26I GTCTC 2 cut(s) 456, 528
AlwI GGATC 3 cut(s) 355, 371, 384
AlwNI CAGNNNCTG 3 cut(s) 659, 707, 892
AoxI GGCC 2 cut(s) 395, 948
ApeKI GCWGC 3 cut(s) 326, 659, 889
ApoI RAATTY 1 cut(s) 733
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 2 cut(s) 230, 389
AspS9I GGNCC 1 cut(s) 395
BamHI GGATCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 761
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 2 cut(s) 36, 143
BbvI GCAGC 3 cut(s) 313, 646, 876
BccI CCATC 5 cut(s) 756, 779, 800, 875, 959
BceAI ACGGC 2 cut(s) 31, 70
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciVI GTATCC 1 cut(s) 855
BclI TGATCA 2 cut(s) 88, 339
BcoDI GTCTC 2 cut(s) 456, 528
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 2 cut(s) 231, 390
BfuI GTATCC 1 cut(s) 855
BisI GCNGC 4 cut(s) 327, 660, 890, 1017
BlsI GCNGC 4 cut(s) 328, 661, 891, 1018
BmcAI AGTACT 2 cut(s) 183, 836
BmgT120I GGNCC 1 cut(s) 395
BmiI GGNNCC 2 cut(s) 378, 763
BpiI GAAGAC 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 1 cut(s) 795
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 943
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 53, 758
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 349, 778
Bse3DI GCAATG 2 cut(s) 639, 738
BseGI GGATG 2 cut(s) 748, 811
BseLI CCNNNNNNNGG 3 cut(s) 23, 53, 758
BseMI GCAATG 2 cut(s) 639, 738
BseMII CTCAG 3 cut(s) 522, 526, 539
BseNI ACTGG 2 cut(s) 349, 778
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 3 cut(s) 313, 646, 876
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 397, 950
BshNI GGYRCC 1 cut(s) 761
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 2 cut(s) 36, 143
BsiSI CCGG 3 cut(s) 24, 213, 944
BslFI GGGAC 3 cut(s) 298, 345, 728
BslI CCNNNNNNNGG 3 cut(s) 23, 53, 758
BsmAI GTCTC 2 cut(s) 456, 528
BsmFI GGGAC 3 cut(s) 298, 345, 728
BsnI GGCC 2 cut(s) 397, 950
Bsp1286I GDGCHC 2 cut(s) 36, 143
Bsp143I GATC 6 cut(s) 88, 339, 347, 376, 508, 646
BspACI CCGC 5 cut(s) 21, 44, 174, 329, 1016
BspANI GGCC 2 cut(s) 397, 950
BspCNI CTCAG 3 cut(s) 523, 525, 538
BspLI GGNNCC 2 cut(s) 378, 763
BspPI GGATC 3 cut(s) 355, 371, 384
BspT107I GGYRCC 1 cut(s) 761
BsrDI GCAATG 2 cut(s) 639, 738
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 2 cut(s) 349, 778
BssAI RCCGGY 1 cut(s) 23
BssMI GATC 6 cut(s) 88, 339, 347, 376, 508, 646
BssNAI GTATAC 1 cut(s) 1003
Bst1107I GTATAC 1 cut(s) 1003
Bst4CI ACNGT 4 cut(s) 10, 86, 364, 867
Bst6I CTCTTC 2 cut(s) 815, 915
BstC8I GCNNGC 1 cut(s) 894
BstDEI CTNAG 4 cut(s) 512, 525, 531, 608
BstF5I GGATG 2 cut(s) 748, 811
BstH2I RGCGCY 2 cut(s) 231, 390
BstHHI GCGC 2 cut(s) 230, 389
BstKTI GATC 6 cut(s) 91, 342, 350, 379, 511, 649
BstMAI GTCTC 2 cut(s) 456, 528
BstMBI GATC 6 cut(s) 88, 339, 347, 376, 508, 646
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNSI RCATGY 1 cut(s) 497
BstSFI CTRYAG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 313, 646, 876
BstV2I GAAGAC 1 cut(s) 540
BstX2I RGATCY 2 cut(s) 347, 376
BstYI RGATCY 2 cut(s) 347, 376
BstZ17I GTATAC 1 cut(s) 1003
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 2 cut(s) 397, 950
BtgZI GCGATG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 748, 811
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 6 cut(s) 91, 226, 306, 342, 551, 863
Cac8I GCNNGC 1 cut(s) 894
CaiI CAGNNNCTG 3 cut(s) 659, 707, 892
CfoI GCGC 2 cut(s) 230, 389
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 182, 835
CviAII CATG 4 cut(s) 108, 170, 290, 494
CviQI GTAC 2 cut(s) 182, 835
DdeI CTNAG 4 cut(s) 512, 525, 531, 608
DpnI GATC 6 cut(s) 90, 341, 349, 378, 510, 648
DpnII GATC 6 cut(s) 88, 339, 347, 376, 508, 646
Eam1104I CTCTTC 2 cut(s) 815, 915
EarI CTCTTC 2 cut(s) 815, 915
EciI GGCGGA 3 cut(s) 10, 33, 189
Ecl136II GAGCTC 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 34
EcoT38I GRGCYC 1 cut(s) 36
FaeI CATG 4 cut(s) 111, 173, 293, 497
FalI AAGNNNNNCTT 2 cut(s) 532, 564
FaqI GGGAC 3 cut(s) 298, 345, 728
FatI CATG 4 cut(s) 107, 169, 289, 493
FauI CCCGC 1 cut(s) 322
FbaI TGATCA 2 cut(s) 88, 339
FblI GTMKAC 1 cut(s) 1002
Fnu4HI GCNGC 4 cut(s) 327, 660, 890, 1017
FokI GGATG 2 cut(s) 735, 818
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 4 cut(s) 327, 660, 890, 1017
FspBI CTAG 1 cut(s) 390
GlaI GCGC 2 cut(s) 229, 388
GluI GCNGC 4 cut(s) 327, 660, 890, 1017
GsuI CTGGAG 1 cut(s) 795
HaeII RGCGCY 2 cut(s) 231, 390
HaeIII GGCC 2 cut(s) 397, 950
HapII CCGG 3 cut(s) 24, 213, 944
HhaI GCGC 2 cut(s) 230, 389
Hin1II CATG 4 cut(s) 111, 173, 293, 497
Hin6I GCGC 2 cut(s) 228, 387
HinP1I GCGC 2 cut(s) 228, 387
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 4 cut(s) 13, 40, 545, 1009
HpaII CCGG 3 cut(s) 24, 213, 944
Hpy166II GTNNAC 5 cut(s) 442, 499, 724, 796, 1003
Hpy188I TCNGA 7 cut(s) 52, 71, 245, 515, 655, 696, 703
Hpy188III TCNNGA 4 cut(s) 29, 37, 200, 878
Hpy8I GTNNAC 5 cut(s) 442, 499, 724, 796, 1003
Hpy99I CGWCG 2 cut(s) 56, 249
HpyAV CCTTC 6 cut(s) 483, 534, 614, 618, 705, 754
HpyCH4III ACNGT 4 cut(s) 10, 86, 364, 867
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 3 cut(s) 427, 632, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 4 cut(s) 512, 525, 531, 608
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 4 cut(s) 111, 173, 293, 497
HspAI GCGC 2 cut(s) 228, 387
Ksp22I TGATCA 2 cut(s) 88, 339
Kzo9I GATC 6 cut(s) 88, 339, 347, 376, 508, 646
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 3 cut(s) 313, 646, 876
MaeI CTAG 1 cut(s) 390
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 3 cut(s) 10, 809, 908
MalI GATC 6 cut(s) 90, 341, 349, 378, 510, 648
MboI GATC 6 cut(s) 88, 339, 347, 376, 508, 646
MboII GAAGA 4 cut(s) 96, 540, 832, 932
MflI RGATCY 2 cut(s) 347, 376
MhlI GDGCHC 2 cut(s) 36, 143
MluCI AATT 2 cut(s) 145, 733
MlyI GAGTC 2 cut(s) 7, 554
MmeI TCCRAC 4 cut(s) 75, 268, 439, 872
MnlI CCTC 9 cut(s) 107, 265, 377, 649, 697, 770, 816, 916, 940
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 889
MspI CCGG 3 cut(s) 24, 213, 944
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeII GATC 6 cut(s) 88, 339, 347, 376, 508, 646
NlaIII CATG 4 cut(s) 111, 173, 293, 497
NlaIV GGNNCC 2 cut(s) 378, 763
NmeAIII GCCGAG 1 cut(s) 839
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 497
OliI CACNNNNGTG 1 cut(s) 410
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 2 cut(s) 40, 1009
PflFI GACNNNGTC 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 758
PkrI GCNGC 4 cut(s) 328, 661, 891, 1018
PleI GAGTC 2 cut(s) 7, 553
PpsI GAGTC 2 cut(s) 7, 553
Psp124BI GAGCTC 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 378, 763
PspPI GGNCC 1 cut(s) 395
PstNI CAGNNNCTG 3 cut(s) 659, 707, 892
PsuI RGATCY 2 cut(s) 347, 376
PsyI GACNNNGTC 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 2 cut(s) 183, 836
RsaNI GTAC 2 cut(s) 182, 835
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 4 cut(s) 327, 660, 890, 1017
Sau3AI GATC 6 cut(s) 88, 339, 347, 376, 508, 646
Sau96I GGNCC 1 cut(s) 395
ScaI AGTACT 2 cut(s) 183, 836
SchI GAGTC 2 cut(s) 7, 554
SduI GDGCHC 2 cut(s) 36, 143
SetI ASST 6 cut(s) 36, 439, 614, 670, 767, 891
SfcI CTRYAG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
Sse9I AATT 2 cut(s) 145, 733
SsiI CCGC 5 cut(s) 21, 44, 174, 329, 1016
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 1 cut(s) 36
TaaI ACNGT 4 cut(s) 10, 86, 364, 867
TaiI ACGT 1 cut(s) 439
TaqI TCGA 4 cut(s) 38, 186, 877, 978
TasI AATT 2 cut(s) 145, 733
TatI WGTACW 2 cut(s) 181, 834
TauI GCSGC 1 cut(s) 1019
TfiI GAWTC 2 cut(s) 40, 1009
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 6 cut(s) 91, 226, 306, 349, 558, 870
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 326, 659, 889
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 96, 987
TspGWI ACGGA 2 cut(s) 6, 262
TspRI CASTG 6 cut(s) 91, 226, 306, 349, 558, 870
Tth111I GACNNNGTC 1 cut(s) 16
Van91I CCANNNNNTGG 1 cut(s) 758
XapI RAATTY 1 cut(s) 733
XceI RCATGY 1 cut(s) 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 755
XmiI GTMKAC 1 cut(s) 1002
XspI CTAG 1 cut(s) 390
ZrmI AGTACT 2 cut(s) 183, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.