MD05G1098500.v1.1

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012239
Physical Location & Seq
Reverse (-)
20458352 .. 20462001
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1098500.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGACAGCTGTCCCTCCGCCTGCGCCAGGCCGAGAGCTCTCCAACCCTCCCACCGACGGCATCTCCAACCTCCGATTCTCCAACCACAGCGACCACCTGCTCGTCTCCTCTTGGGACAAGACCGTCCGATTGTACGACGCCAGCGCCAATGTGCTGCGAGGAGAGTTCATGCACGGAGGTCCCGTTCTCGATTGCTGCTTCCACGATGATTCCTCTGGGTTCAGTGCCGGTGCCGACAACACTGTCAGGCGGTTTGTATTCAGCTCCAGTAAGGAAGACATTTTAGGAAGGCACGACGCGCCGGTGCGTTGCATTGAATACTCTTATGCAGCAGGGCAGTTGATTACTGGTAGTTGGGATAAAACTGTGAAGTGTTGGGACCCTAGAGGCGCAAGTGGACAGGAGAGAACTCTTGTTGGGTCATACCCACAACCCGAGCGTGTTTATTCTCTTTCACTTGTGGGGAACCGATTAGTGGTTGCAACCGCCGGAAGACATGTCAATATCTATGATTTGAGAAATATGTCCCAGCCTGAGCAACGGAGGGAGTCTTCCTTAAAGTATCAAACTCGATGTGTGCGCTGTTATCCCAATGGAACAGGTTATGCTCTCAGTTCTGTTGAAGGACGGGTTGCAATGGAGTTTTTTGATCTCTCCGAGGCTAGTCAAACTAAAAAATATGCATTTAAGTGTCATAGAAAATCAGAGGCTGGAAGGGACATTGTGTATCCAGTAAATGCAATTGCATTCCACCCCATCTATGGTACTTTTGCTACTGGAGGTTGTGATGGTTATGTGAACGTATGGGACGGGAACAACAAGAAGAGGCTGTATCAGTACTCGAAGTACCCAACAAGCATTGCGGCACTTTCTTTTAGCAGAGATGGCCGCCTATTGGCTGTGGCATCAAGTTACACATATGAAGAGGGAGATAAACCCCATGAGCCAGATGCCATCTTTGTTCGAAGTGTAAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTGTACCCTAATCCGCCAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

342

Amino Acids

37.89

Weight (kDa)

8.51

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 18 - 231 6.3e-17 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 19 - 128 3.4e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 63 - 225 2.8e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 75 - 304 4.8e-24 THOC3 beta-propeller domain
WD40 PF00400 95 - 127 4.3e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 146 - 293 3.2e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 146 - 295 7.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 157 - 304 3.4e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 242 - 304 4e-06 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 247 - 303 3.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 105
AasI GACNNNNNNGTC 2 cut(s) 122, 242
Acc36I ACCTGC 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 230
AccII CGCG 1 cut(s) 299
AciI CCGC 6 cut(s) 17, 250, 486, 863, 889, 1016
AcoI YGGCCR 1 cut(s) 886
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 5 cut(s) 134, 766, 839, 848, 1007
AfiI CCNNNNNNNGG 5 cut(s) 26, 56, 475, 761, 895
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 2 cut(s) 317, 623
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 3 cut(s) 8, 37, 264
AluI AGCT 3 cut(s) 8, 37, 264
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 28, 886
ApeKI GCWGC 3 cut(s) 154, 195, 329
AspLEI GCGC 5 cut(s) 25, 146, 301, 392, 582
AspS9I GGNCC 2 cut(s) 179, 379
AsuII TTCGAA 1 cut(s) 964
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 179, 379
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 3 cut(s) 282, 499, 543
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 141, 182, 341
BccI CCATC 4 cut(s) 764, 782, 878, 962
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 27
BciVI GTATCC 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 384, 663
BfoI RGCGCY 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 738
BisI GCNGC 5 cut(s) 155, 196, 330, 864, 889
BlsI GCNGC 5 cut(s) 156, 197, 331, 865, 890
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 179, 379
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 2 cut(s) 179, 379
BmiI GGNNCC 5 cut(s) 181, 232, 380, 381, 467
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 3 cut(s) 69, 914, 940
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 3 cut(s) 282, 499, 543
BpmI CTGGAG 2 cut(s) 250, 798
Bpu10I CCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 964
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 657
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 168, 198
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 56, 475, 761, 895
Bse118I RCCGGY 2 cut(s) 227, 301
Bse1I ACTGG 4 cut(s) 267, 352, 731, 781
Bse3DI GCAATG 2 cut(s) 642, 858
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 657
BseLI CCNNNNNNNGG 5 cut(s) 26, 56, 475, 761, 895
BseMI GCAATG 2 cut(s) 642, 858
BseMII CTCAG 2 cut(s) 525, 625
BseNI ACTGG 4 cut(s) 267, 352, 731, 781
BseRI GAGGAG 2 cut(s) 97, 174
BseXI GCAGC 3 cut(s) 141, 182, 341
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 2 cut(s) 30, 888
BshNI GGYRCC 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 3 cut(s) 228, 302, 489
BslFI GGGAC 6 cut(s) 128, 165, 392, 511, 731, 821
BslI CCNNNNNNNGG 5 cut(s) 26, 56, 475, 761, 895
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 6 cut(s) 128, 165, 392, 511, 731, 821
BsmI GAATGC 1 cut(s) 746
BsnI GGCC 2 cut(s) 30, 888
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 964
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 1 cut(s) 649
BspACI CCGC 6 cut(s) 17, 250, 486, 863, 889, 1016
BspANI GGCC 2 cut(s) 30, 888
BspCNI CTCAG 2 cut(s) 526, 624
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 5 cut(s) 181, 232, 380, 381, 467
BspMI ACCTGC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 964
BspT107I GGYRCC 1 cut(s) 230
BsrDI GCAATG 2 cut(s) 642, 858
BsrFI RCCGGY 2 cut(s) 227, 301
BsrI ACTGG 4 cut(s) 267, 352, 731, 781
BssAI RCCGGY 2 cut(s) 227, 301
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 1 cut(s) 649
BssNI GRCGYC 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 124, 244, 367
Bst6I CTCTTC 2 cut(s) 818, 918
BstACI GRCGYC 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 964
BstC8I GCNNGC 3 cut(s) 21, 142, 1020
BstDEI CTNAG 2 cut(s) 534, 611
BstENI CCTNNNNNAGG 1 cut(s) 24
BstFNI CGCG 1 cut(s) 299
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 5 cut(s) 25, 146, 301, 392, 582
BstKTI GATC 1 cut(s) 652
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 1 cut(s) 649
BstMWI GCNNNNNNNGC 2 cut(s) 298, 885
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 3 cut(s) 141, 182, 341
BstV2I GAAGAC 3 cut(s) 282, 499, 543
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 2 cut(s) 30, 888
BtsIMutI CAGTG 2 cut(s) 229, 240
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 21, 142, 1020
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 5 cut(s) 25, 146, 301, 392, 582
Cfr10I RCCGGY 2 cut(s) 227, 301
Cfr13I GGNCC 2 cut(s) 179, 379
CseI GACGC 2 cut(s) 146, 305
Csp6I GTAC 5 cut(s) 133, 765, 838, 847, 1006
CviAII CATG 3 cut(s) 169, 497, 941
CviQI GTAC 5 cut(s) 133, 765, 838, 847, 1006
DdeI CTNAG 2 cut(s) 534, 611
DpnI GATC 1 cut(s) 651
DpnII GATC 1 cut(s) 649
DrdI GACNNNNNNGTC 2 cut(s) 122, 242
DseDI GACNNNNNNGTC 2 cut(s) 122, 242
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 2 cut(s) 818, 918
EarI CTCTTC 2 cut(s) 818, 918
EciI GGCGGA 2 cut(s) 6, 1005
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 179, 379
Eco53kI GAGCTC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 434
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 24
EcoO109I RGGNCCY 2 cut(s) 179, 379
EcoRII CCWGG 1 cut(s) 25
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 3 cut(s) 172, 500, 944
FaqI GGGAC 6 cut(s) 128, 165, 392, 511, 731, 821
FatI CATG 3 cut(s) 168, 496, 940
FauNDI CATATG 1 cut(s) 919
Fnu4HI GCNGC 5 cut(s) 155, 196, 330, 864, 889
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 5 cut(s) 155, 196, 330, 864, 889
FspBI CTAG 2 cut(s) 384, 663
GlaI GCGC 5 cut(s) 24, 145, 300, 391, 581
GluI GCNGC 5 cut(s) 155, 196, 330, 864, 889
GsaI CCCAGC 1 cut(s) 532
GsuI CTGGAG 2 cut(s) 250, 798
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 2 cut(s) 30, 888
HapII CCGG 3 cut(s) 228, 302, 489
HgaI GACGC 2 cut(s) 146, 305
HhaI GCGC 5 cut(s) 25, 146, 301, 392, 582
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 3 cut(s) 172, 500, 944
Hin6I GCGC 5 cut(s) 23, 144, 299, 390, 580
HinP1I GCGC 5 cut(s) 23, 144, 299, 390, 580
HinfI GANTC 3 cut(s) 75, 209, 548
HpaII CCGG 3 cut(s) 228, 302, 489
Hpy166II GTNNAC 3 cut(s) 398, 799, 1006
Hpy188I TCNGA 4 cut(s) 74, 128, 658, 706
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 3 cut(s) 398, 799, 1006
Hpy99I CGWCG 3 cut(s) 59, 140, 299
HpyAV CCTTC 3 cut(s) 282, 617, 708
HpyCH4III ACNGT 3 cut(s) 124, 244, 367
HpyCH4IV ACGT 1 cut(s) 801
HpyCH4V TGCA 8 cut(s) 172, 312, 329, 482, 635, 683, 740, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 885
HpyF3I CTNAG 2 cut(s) 534, 611
HpySE526I ACGT 1 cut(s) 801
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 3 cut(s) 172, 500, 944
HspAI GCGC 5 cut(s) 23, 144, 299, 390, 580
KflI GGGWCCC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 649
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 3 cut(s) 141, 182, 341
LweI GCATC 3 cut(s) 69, 914, 940
MaeI CTAG 2 cut(s) 384, 663
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 1 cut(s) 911
MalI GATC 1 cut(s) 651
MboI GATC 1 cut(s) 649
MboII GAAGA 5 cut(s) 287, 504, 543, 835, 935
MfeI CAATTG 1 cut(s) 741
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 741
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 3 cut(s) 66, 90, 105
Mph1103I ATGCAT 1 cut(s) 685
MseI TTAA 2 cut(s) 557, 687
MslI CAYNNNNRTG 1 cut(s) 688
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 3 cut(s) 228, 302, 489
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 746
MvaI CCWGG 1 cut(s) 27
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 2 cut(s) 298, 885
NdeI CATATG 1 cut(s) 919
NdeII GATC 1 cut(s) 649
NlaIII CATG 3 cut(s) 172, 500, 944
NlaIV GGNNCC 5 cut(s) 181, 232, 380, 381, 467
NmeAIII GCCGAG 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 1 cut(s) 964
PaqCI CACCTGC 1 cut(s) 105
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 746
PfeI GAWTC 2 cut(s) 75, 209
PkrI GCNGC 5 cut(s) 156, 197, 331, 865, 890
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PpuMI RGGWCCY 2 cut(s) 179, 379
PscI ACATGT 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 179, 379
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 5 cut(s) 181, 232, 380, 381, 467
PspPI GGNCC 2 cut(s) 179, 379
PspPPI RGGWCCY 2 cut(s) 179, 379
PstNI CAGNNNCTG 1 cut(s) 710
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 134, 766, 839, 848, 1007
RsaNI GTAC 5 cut(s) 133, 765, 838, 847, 1006
RseI CAYNNNNRTG 1 cut(s) 688
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 557, 687
SatI GCNGC 5 cut(s) 155, 196, 330, 864, 889
Sau3AI GATC 1 cut(s) 649
Sau96I GGNCC 2 cut(s) 179, 379
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 3 cut(s) 69, 914, 940
SfuI TTCGAA 1 cut(s) 964
SgrAI CRCCGGYG 1 cut(s) 301
SinI GGWCC 2 cut(s) 179, 379
SmiMI CAYNNNNRTG 1 cut(s) 688
Sse9I AATT 1 cut(s) 741
SsiI CCGC 6 cut(s) 17, 250, 486, 863, 889, 1016
SspMI CTAG 2 cut(s) 384, 663
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 3 cut(s) 124, 244, 367
TaiI ACGT 1 cut(s) 804
TaqI TCGA 4 cut(s) 189, 571, 842, 964
TasI AATT 1 cut(s) 741
TatI WGTACW 1 cut(s) 837
TauI GCSGC 2 cut(s) 866, 891
TfiI GAWTC 2 cut(s) 75, 209
Tru1I TTAA 2 cut(s) 557, 687
Tru9I TTAA 2 cut(s) 557, 687
TscAI CASTG 2 cut(s) 229, 247
TseI GCWGC 3 cut(s) 154, 195, 329
TspDTI ATGAA 2 cut(s) 157, 936
TspGWI ACGGA 2 cut(s) 189, 556
TspRI CASTG 2 cut(s) 229, 247
VpaK11BI GGWCC 2 cut(s) 179, 379
XagI CCTNNNNNAGG 1 cut(s) 24
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XspI CTAG 2 cut(s) 384, 663
ZrmI AGTACT 1 cut(s) 839
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.