Rh6BG053900

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012239
Physical Location & Seq
Reverse (-)
8776511 .. 8780205
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG053900.1

Sequence Viewer

Length: 762 bp
ATGACAGCTGTCCCTCCGCCGTCCTCCGGCCGAGAGCTCTCCAACCCCCCCGCCGACGGCGTCTCCAACCTCCGCTTCTCCAACCACAGCGATCACCTGCTCGTCTCCTCTTGGGATAAGACCGTCCGATTGTACGACGCCACCGCCAATGTGCTCCGCGGAGAGTTCACGCACGGCAGCCCCGTCCTCGATTGCTGCTTCCACGACGACTCCTCCGGCTTCAGCGCCAGCGCCGACAACACCGTCCGCCGATTCGTTTTCAACTCCAATAAGGAAGATATTATAGGAAAGCACGACGCACCGGTTCGTTGCGTTGAGTACTCGTATGCAGCAGGGCAACTGATCACCGGTAGTTGGGATAAGACACTCAAGTGTTGGGATCCTAGAGGGGCAAATGGACAAGAGCGTACTCTTGTTGGGACTTATCAACAGCCGGAGCGTGTTTATTCTCTTTCTCTGGTCGGAAATCGTTTAGTGGTTGCAACTGCTGGAAGACATGTGAATGTCTATGATCTGAGAAATATGTCACAGCCTGAGCAACGAAGGGAATCTTCATTGAAGTATCAAACTCGATGTGTGCGCTGTTATCCTAATGGAACAGGTTATGCTCTTAGTTCTGTTGAAGGACGGGTTGCAATGGAATTCTTTGATCTCTCAGAGGCTAGTCAAACCAAAAAATATGCATTCAAGTGTCATAGAAAATCAGAGGCTGGAAGGGACATTGTATATCCAGTAAATGCAATTGCATTTCATCCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

253

Amino Acids

28.21

Weight (kDa)

8.44

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 18 - 132 5.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 19 - 231 3.7e-18 WDR5 beta-propeller domain
WD40_Prp19 PF24814 19 - 233 2.8e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 19 - 128 9.3e-09 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 19 - 164 8.2e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 20 - 127 2.2e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 75 - 222 8e-12 THOC3 beta-propeller domain
WD40 PF00400 97 - 127 7.4e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 105
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 105
AccII CGCG 1 cut(s) 159
AciI CCGC 7 cut(s) 17, 51, 73, 144, 157, 159, 247
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 60, 138
AfaI GTAC 3 cut(s) 134, 320, 409
AfiI CCNNNNNNNGG 3 cut(s) 26, 56, 354
AflIII ACRYGT 1 cut(s) 496
AgeI ACCGGT 2 cut(s) 301, 347
AgsI TTSAA 4 cut(s) 262, 559, 623, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 2 cut(s) 8, 37
AluI AGCT 2 cut(s) 8, 37
Alw21I GWGCWC 2 cut(s) 39, 156
Alw26I GTCTC 2 cut(s) 67, 109
AlwI GGATC 2 cut(s) 374, 387
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 3 cut(s) 177, 195, 329
ApoI RAATTY 1 cut(s) 641
AsiGI ACCGGT 2 cut(s) 301, 347
AspLEI GCGC 3 cut(s) 227, 233, 582
AsuHPI GGTGA 2 cut(s) 86, 337
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 39, 156
BbvI GCAGC 3 cut(s) 182, 189, 341
BceAI ACGGC 3 cut(s) 4, 73, 190
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 2 cut(s) 67, 109
BfaI CTAG 2 cut(s) 384, 663
BfoI RGCGCY 2 cut(s) 228, 234
BfuAI ACCTGC 1 cut(s) 105
BisI GCNGC 3 cut(s) 178, 196, 330
BlsI GCNGC 3 cut(s) 179, 197, 331
BmcAI AGTACT 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 381
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 353
BsaHI GRCGYC 2 cut(s) 60, 138
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 2 cut(s) 301, 347
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 194, 197, 224, 227
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 56, 354
Bse118I RCCGGY 2 cut(s) 301, 347
Bse1I ACTGG 1 cut(s) 731
Bse3DI GCAATG 1 cut(s) 642
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 751
BseLI CCNNNNNNNGG 3 cut(s) 26, 56, 354
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 3 cut(s) 506, 525, 669
BseNI ACTGG 1 cut(s) 731
BseRI GAGGAG 2 cut(s) 97, 202
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 3 cut(s) 182, 189, 341
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 1 cut(s) 30
BshTI ACCGGT 2 cut(s) 301, 347
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 39, 156
BsiSI CCGG 5 cut(s) 27, 216, 302, 348, 434
BslFI GGGAC 2 cut(s) 433, 731
BslI CCNNNNNNNGG 3 cut(s) 26, 56, 354
BsmAI GTCTC 2 cut(s) 67, 109
BsmBI CGTCTC 2 cut(s) 67, 109
BsmFI GGGAC 2 cut(s) 433, 731
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 39, 156
Bsp143I GATC 5 cut(s) 91, 342, 379, 511, 649
BspACI CCGC 7 cut(s) 17, 51, 73, 144, 157, 159, 247
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 3 cut(s) 507, 526, 668
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 374, 387
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 2 cut(s) 301, 347
BsrI ACTGG 1 cut(s) 731
BssAI RCCGGY 2 cut(s) 301, 347
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 5 cut(s) 91, 342, 379, 511, 649
BssNI GRCGYC 2 cut(s) 60, 138
Bst4CI ACNGT 2 cut(s) 124, 244
BstACI GRCGYC 2 cut(s) 60, 138
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 4 cut(s) 515, 534, 611, 655
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 751
BstFNI CGCG 1 cut(s) 159
BstH2I RGCGCY 2 cut(s) 228, 234
BstHHI GCGC 3 cut(s) 227, 233, 582
BstKTI GATC 5 cut(s) 94, 345, 382, 514, 652
BstMAI GTCTC 2 cut(s) 67, 109
BstMBI GATC 5 cut(s) 91, 342, 379, 511, 649
BstMCI CGRYCG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 8
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 3 cut(s) 182, 189, 341
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 28
BsuRI GGCC 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 751
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 229
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 3 cut(s) 227, 233, 582
Cfr10I RCCGGY 2 cut(s) 301, 347
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 3 cut(s) 49, 146, 305
Csp6I GTAC 3 cut(s) 133, 319, 408
CspAI ACCGGT 2 cut(s) 301, 347
CviAII CATG 2 cut(s) 497, 757
CviJI RGCY 9 cut(s) 8, 30, 37, 180, 219, 433, 532, 662, 710
CviKI_1 RGCY 9 cut(s) 8, 30, 37, 180, 219, 433, 532, 662, 710
CviQI GTAC 3 cut(s) 133, 319, 408
DdeI CTNAG 4 cut(s) 515, 534, 611, 655
DpnI GATC 5 cut(s) 93, 344, 381, 513, 651
DpnII GATC 5 cut(s) 91, 342, 379, 511, 649
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 28
EagI CGGCCG 1 cut(s) 28
EciI GGCGGA 2 cut(s) 6, 236
Ecl136II GAGCTC 1 cut(s) 37
EclXI CGGCCG 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 39
Eco52I CGGCCG 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 641
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 39
Esp3I CGTCTC 2 cut(s) 67, 109
FaeI CATG 2 cut(s) 500, 760
FalI AAGNNNNNCTT 2 cut(s) 535, 567
FaqI GGGAC 2 cut(s) 433, 731
FatI CATG 2 cut(s) 496, 756
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 3 cut(s) 178, 196, 330
FokI GGATG 1 cut(s) 738
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 178, 196, 330
FspBI CTAG 2 cut(s) 384, 663
GlaI GCGC 3 cut(s) 226, 232, 581
GluI GCNGC 3 cut(s) 178, 196, 330
HaeII RGCGCY 2 cut(s) 228, 234
HaeIII GGCC 1 cut(s) 30
HapII CCGG 5 cut(s) 27, 216, 302, 348, 434
HgaI GACGC 3 cut(s) 49, 146, 305
HhaI GCGC 3 cut(s) 227, 233, 582
Hin1I GRCGYC 2 cut(s) 60, 138
Hin1II CATG 2 cut(s) 500, 760
Hin6I GCGC 3 cut(s) 225, 231, 580
HinP1I GCGC 3 cut(s) 225, 231, 580
HinfI GANTC 3 cut(s) 209, 252, 548
HpaII CCGG 5 cut(s) 27, 216, 302, 348, 434
HphI GGTGA 2 cut(s) 86, 337
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 5 cut(s) 128, 464, 516, 658, 706
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 4 cut(s) 59, 140, 209, 299
HpyAV CCTTC 3 cut(s) 537, 617, 708
HpyCH4III ACNGT 2 cut(s) 124, 244
HpyCH4V TGCA 6 cut(s) 329, 482, 635, 683, 740, 746
HpyF3I CTNAG 4 cut(s) 515, 534, 611, 655
Hsp92I GRCGYC 2 cut(s) 60, 138
Hsp92II CATG 2 cut(s) 500, 760
HspAI GCGC 3 cut(s) 225, 231, 580
Ksp22I TGATCA 1 cut(s) 342
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 5 cut(s) 91, 342, 379, 511, 649
LmnI GCTCC 2 cut(s) 159, 436
Lsp1109I GCAGC 3 cut(s) 182, 189, 341
MaeI CTAG 2 cut(s) 384, 663
MaeIII GTNAC 1 cut(s) 525
MalI GATC 5 cut(s) 93, 344, 381, 513, 651
MboI GATC 5 cut(s) 91, 342, 379, 511, 649
MboII GAAGA 3 cut(s) 287, 504, 543
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 39, 156
MluCI AATT 2 cut(s) 641, 741
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 4 cut(s) 66, 90, 105, 442
MnlI CCTC 9 cut(s) 24, 34, 80, 118, 197, 223, 380, 652, 700
Mph1103I ATGCAT 1 cut(s) 685
MslI CAYNNNNRTG 3 cut(s) 370, 501, 688
MspA1I CMGCKG 2 cut(s) 8, 159
MspI CCGG 5 cut(s) 27, 216, 302, 348, 434
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 683
MvnI CGCG 1 cut(s) 159
NdeII GATC 5 cut(s) 91, 342, 379, 511, 649
NlaIII CATG 2 cut(s) 500, 760
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 105
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 2 cut(s) 252, 548
PflFI GACNNNGTC 1 cut(s) 59
PinAI ACCGGT 2 cut(s) 301, 347
PkrI GCNGC 3 cut(s) 179, 197, 331
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PscI ACATGT 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 134, 320, 409
RsaNI GTAC 3 cut(s) 133, 319, 408
RseI CAYNNNNRTG 3 cut(s) 370, 501, 688
SacI GAGCTC 1 cut(s) 39
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 3 cut(s) 178, 196, 330
Sau3AI GATC 5 cut(s) 91, 342, 379, 511, 649
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 2 cut(s) 39, 156
SetI ASST 5 cut(s) 10, 39, 72, 99, 604
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SmiMI CAYNNNNRTG 3 cut(s) 370, 501, 688
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 641, 741
SsiI CCGC 7 cut(s) 17, 51, 73, 144, 157, 159, 247
SspMI CTAG 2 cut(s) 384, 663
SstI GAGCTC 1 cut(s) 39
TaaI ACNGT 2 cut(s) 124, 244
TaqI TCGA 2 cut(s) 189, 571
TasI AATT 2 cut(s) 641, 741
TatI WGTACW 1 cut(s) 318
TfiI GAWTC 2 cut(s) 252, 548
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 3 cut(s) 177, 195, 329
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 2 cut(s) 543, 740
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 500
XspI CTAG 2 cut(s) 384, 663
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.